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Temperature and thermodynamic deformation analysis of the rotors on a twin screw multiphase pump with high gas volume fractions

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第9期12卷 720-730页

作者： Tie-yu GAO dong-fang yang Feng CAO Jin-cheng JIAO School of Energy and Power Engineering Xi'an Jiaotong University Xi'an 710049 China

The pressure increasing process within a twin screw multiphase pump, under the condition of high gas volume fractions (GVFs), induces large temperature and pressure changes that cause the rotors to deform. Rotor deformations heavily influence the backflow of the multiphase fluid through clearances within the twin screw multiphase pump and these deformations may even lead to pump failures. An accurate temperature and pressure distribution on the screw rotors need be obtained before the deformation analysis can be carried out. By means of small temperature and pressure sensors embedded into the groove at the root of the rotors, the temperatures of 12 points on the rotors and the pressure distributions of a twin screw multiphase pump under high GVFs conditions were recorded. Temperature test results were adopted to perform a heat transfer analysis for determining the temperature distribution on the screw rotors. Then deformation analyses, including thermal deformation, force deformation, and total deformation, were conducted according to the pressure and temperature distributions. Deformation analysis for different materials was also conducted under the same boundary conditions. A material was suggested for the manufacturing of rotors in a twin screw multiphase pump under the condition of high gas volume fractions.

关键词： Multiphase pump Twin screw rotor Thermal deformation Finite element analysis

GPC3 fused to an alpha epitope of HBsAg acts as an immune target against hepatocellular carcinoma associated with hepatitis B virus

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 164-170页

作者： Jun-Wen yang,dong-Ye yang,fang-Gen Lu,Cai-Hong Li,Hui Chen,Ning Xie and Xin Zhao Department of Digestive Diseases,Second Xiangya Hospital,Central South University,Changsha 410011,China Department of Digestive Diseases Second Xiangya Hospital Central South University Changsha 410011 China

BACKGROUND:The incidence of hepatocellular carcinoma (HCC)in China is closely related to the population infected with hepatitis B virus(HBV).HCC cells with HBV secrete soluble HBsAg into blood but do not express it on the cell membrane This study aimed to construct and investigate a new glycosyl phosphatidylinositol(GPI)-anchored protein(GPC3+α+EGFP) as a DNA vaccine against HCC associated with HBV. METHODS:A recombinant plasmid(pcDNA3.1(+)/GPC3+ α+EGFP)was constructed and verified by restriction endo nuclease digestion and ***3.1(+)/GPC3+α+ EGFP was transfected into HepG2 cells(experimental group) using lipofectamine ***-N1-transfected HepG2 cells were used as a negative control,and non-transfected HepG2 cells sreved as a blank ***2 cells that steadily expressed the fusion protein GPC3+α+EGFP were screened by G418,propagated,and co-cultured with lymphocytes from healthy *** proliferation was measured by the classic sulforhodamine B *** was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL),and Fas gene transcription was determined by quantitative fluorescent PCR. RESULTS:The pcDNA3.1(+)/GPC3+α+EGFP plasmid was successfully *** the experimental group,green fluorescence was observed at the cell periphery and in the cytoplasm,whereas in the negative control group,fluorescence was evenly distributed throughout the *** of the experimental group significantly decreased after 72 hours compared to the negative and blank control ***,the number of apoptotic cells was statistically different among the three groups as determined by a contingency table Chisquare test;the experimental group had the highest incidence of *** gene transcription in the experimental group was higher than in the two control groups,and an increasing trend with time in the experimental group was observed. CONCLUSION:A chimeric,membrane-anchored protein, GPC3+α+EGFP,localized

关键词： HBsAg-αepitope glypican 3 hepatocellular carcinoma hepatitis B virus protein engineering

Analyses and Solutions of Specific Cases in Spatial Angles Calculation

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Computer Aided Drafting,Design and Manufacturing 2006年第1期16卷 57-62页

作者： ZHANG Ai-mei QIU Yi SUN Bo-lu fang dong-yang School of Mechanical Engineering Zhengzhou University Zhengzhou 450002 China

Aiming at the specific cases existing in spatial angles calculation the paper investigates five types of them： lines in specific orientations, planes in specific orientations, two lines defining a plane and angles between them, orientations and relevant parameters of a line lying on a plane, and orientations and relevant parameters of a plane passing through a line. The results are presented. The strategy presented provides a firm theoretic basis for the application of the new method of spatial angles calculations.

关键词： engineering graphics spatial angles automatic calculation specific cases

Optical Pulse Compression Based on High-doped Erbium Fiber Amplifier and Standard Single-mode Fiber

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Semiconductor Photonics and Technology 2008年第2期14卷 90-94页

作者： REN Jing JIA dong-fang LIU yang LI Ya-bin Optoelectronic Information Science and Technology Lab College of PrecisionInstrument and Optoelectronics Engineering Tianjin University Tianjin 300072 CHN

Proposed is a novel optical pulse compression technique based on high-doped erbium fiber amplifier and standard single-mode fiber(SMF). We used the amplifier with the erbium ion concentration of 6.3×10-3 to amplify a hyperbolic secant pulse from a regeneratively mode-locked fiber laser. The central wavelength, pulsewidth and peak power of the pulse are 1 550 nm, 12.5 ps and 3 mW, respectively. Then the amplified pulse with peak power level corresponding to a higher-order soliton is compressed when it propagates through a 3-km-long single-mode fiber. Studied are the compressed pulses under different pump powers and fiber lengths. The results show that it can get a narrower pulse, and solve the difficulty that pulses at low power can not be compressed directly in the fiber. And the construct is compact.

关键词： high-doped erbium fiber amplifier soliton single mode fiber(SMF) pulse compression

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脚手架钢管矫直除锈机的设计

煤矿机械 2012年第10期33卷 33-34页

作者：方东阳高琳郑州大学机械工程学院郑州450001

设计的钢管矫直除锈机以电机为动力,利用蜗轮蜗杆、联轴器、锥齿轮和链传动等作为传动机构;由弹簧和单叶双曲面辊系结构通过调节压力实现矫直功能,以滚筒带动钢刷实现钢管表面的除锈效果;该设备结构简单,易于操作,较好地解决了生产中的... 详细信息

设计的钢管矫直除锈机以电机为动力,利用蜗轮蜗杆、联轴器、锥齿轮和链传动等作为传动机构;由弹簧和单叶双曲面辊系结构通过调节压力实现矫直功能,以滚筒带动钢刷实现钢管表面的除锈效果;该设备结构简单,易于操作,较好地解决了生产中的问题。

关键词：脚手架钢管矫直除锈

旱作转甜菜碱醛脱氢酶基因水稻开花后根系衰老生理特性研究

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干旱地区农业研究 2007年第4期25卷 221-224,234页

作者：孙耀中东方阳河北科技师范学院生命科学系河北昌黎066600

采用田间试验与盆栽试验相结合的方法,研究了旱作条件下转甜菜碱醛脱氢酶(BADH)基因水稻开花后根系生理特性的变化。结果表明:在旱作条件下,转BADH基因水稻开花后根系能保持较高的SOD、POD活性;膜脂过氧化产物MDA含量较低;可溶性糖含量... 详细信息

采用田间试验与盆栽试验相结合的方法,研究了旱作条件下转甜菜碱醛脱氢酶(BADH)基因水稻开花后根系生理特性的变化。结果表明:在旱作条件下,转BADH基因水稻开花后根系能保持较高的SOD、POD活性;膜脂过氧化产物MDA含量较低;可溶性糖含量较高;在干旱胁迫下根系活力高于受体亲本(中花8号),表明转BADH基因水稻生育后期能够有效地调节根系细胞的渗透势,减少有害离子对根系的伤害,从而延缓根系衰老,使根系在产量形成期维持较强的生理活性。

关键词：水稻转BADH基因旱作根系生理特性

Modeling tactile perceived intensity for vibration from a coin eccentric rotating mass motor

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Science China Technological Sciences 2025年

作者： Yun fang Jingchen HUANG Yujun WU Jiaqi dong Weichao GUO Xinjun SHENG State Key Laboratory of Mechanical System and Vibration School of Mechanical Engineering Shanghai Jiao Tong University Meta Robotics Institute Shanghai Jiao Tong University

Establishing the relationship between vibrotactile parameters and perceived intensity（PI） is of great significance in the regulation of desired sensations, which contribute to haptic interfaces in practical applications. Coin eccentric rotating mass（ERM） motors are routinely utilized due to their lightweight design and efficient ability to evoke strong tactile sensations. By adjusting input voltages, ERMs can yield different stimuli, but the effects of their physical properties on PI remain ***, we developed the physical model of the ERM-skin system and optimized the parameters affecting PI. Moreover, the relationship between stimulus and perception was derived based on Fechner?s law. Three experiments were conducted on fifteen subjects（ten males and five females, aged 24.40 ± 2.87 years） to identify the vibration parameters and corresponding PI to verify the proposed PI model. ERMs in this study were attached to phantom skin/forearm with slim adhesive tape to minimize the interference. Experiment 1 performed vibration calibration with ERM attached to the skin phantom to simulate the actual configuration. Then, the relative and absolute PI of subjects on specific stimuli were acquired in Experiments 2 and *** fitting reliability of the proposed PI model was evaluated on fifteen subjects with R2of 0.73 ± 0.07 and root mean squared error（RMSE） of 1.35 ± 0.28. Overall, the proposed PI model established the quantitative relationship between stimulus parameters（vibration parameters and physical parameters） and subjective PI, which provided theoretical support for ERM optimization and vibrotactile modulation.

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大豆14-3-3蛋白与转录因子蛋白GmMYB173的互作

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作物学报 2016年第10期42卷 1419-1428页

作者：董萌高友菲韩天富东方阳蒋炳军河北科技师范学院生命科技学院河北秦皇岛066000 中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室北京100081

14-3-3蛋白家族在真核生物中普遍存在,可与其他蛋白相互作用,调控多种生理生化过程。MYB基因家族作为植物中最大的一类转录因子,广泛参与了植物的生长发育和代谢调控。本研究通过分析1个从自贡冬豆中克隆的MYB转录因子Gm MYB173的亚细... 详细信息

14-3-3蛋白家族在真核生物中普遍存在,可与其他蛋白相互作用,调控多种生理生化过程。MYB基因家族作为植物中最大的一类转录因子,广泛参与了植物的生长发育和代谢调控。本研究通过分析1个从自贡冬豆中克隆的MYB转录因子Gm MYB173的亚细胞定位情况,发现Gm MYB173在细胞核中特异表达;序列分析发现Gm MYB173与Gm MYB176相似,具有1个14-3-3蛋白的潜在结合结构域,即p ST结合结构域。通过重叠延伸PCR(SOE-PCR)删除了Gm MYB173序列中p ST结合结构域编码序列,发现Gm MYB173细胞核表达特异性消失。酵母双杂交互作分析表明,大豆基因组中所有具有表达的16个14-3-3蛋白Gm SGF14a^Gm SGF14p均能与Gm MYB173互作。β-半乳糖苷酶活性分析发现,与Gm MYB173互作最强的是Gm SGF14n,其次是Gm SGF14k、Gm SGF14e和Gm SGF14o。这些结果说明14-3-3蛋白不仅与Gm MYB173互作,且可能调控其在细胞内的定位,有助于研究14-3-3蛋白与Gm MYB173的互作关系及其在大豆生长发育中的作用。

关键词： 14-3-3蛋白 Gm MYB173 亚细胞定位酵母双杂交

超声声速匹配技术对男性输精管外径测量值的探讨

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中国伤残医学 2014年第19期22卷 9-11页

作者：董杨芳福建医科大学附属福州市第一医院超声科福建福州350000

目的：探讨正常男性精索静脉曲张男性输精管声像图特征及外径。方法：使用超声声速匹配技术探查输精管声像图特征，探查48例精索静脉曲张伴反流患者阴囊彩超，与48例无精索静脉曲张正常男性对比，比较输精管睾丸段外径测量值。结果：超... 详细信息

目的：探讨正常男性精索静脉曲张男性输精管声像图特征及外径。方法：使用超声声速匹配技术探查输精管声像图特征，探查48例精索静脉曲张伴反流患者阴囊彩超，与48例无精索静脉曲张正常男性对比，比较输精管睾丸段外径测量值。结果：超声声速匹配技术可清晰辨认输精管睾丸段结构。精索静脉曲张伴反流患者左侧输精管睾丸部管腔外径约1．58±0．46mm，右侧1．54±0．42mm。结论：超声声速匹配技术能清晰、准确地显示输精管睾丸部、阴囊部。可作为男性不孕患者输精管疾病的有效检查方法。正常患者与精索静脉曲张患者的输精管外径差别无统计学意义。

关键词：声速匹配技术输精管精索静脉曲张