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Expression Analysis of HMW-GS 1Bx14 and 1By15 in Wheat Varieties and Transgenic Research of 1By15 Gene

Agricultural Sciences in China 2006年第10期5卷 725-735页

作者： XU Tao ZHANG Xue-yong dong yu-shen Key Laboratory of Crop Germplasm and Biotechnology Ministry of Agriculture/Institute of Crop Sciences Chinese Academy of AgriculturalSciences The National Facility for Crop Gene Resources and Genetic Improvement Beijing 100081 P.R.China

High-molecular-weight glutenin subunits （HMW-GSs）, one class of seed storage proteins in wheat, play an important role in determining bread-making quality of flour. More and more proves support that HMW-GS- 1Bx 14 and - 1Bx 15 subunits are strongly positively associated with good bread-making and excellent noodle-making quality. The two subunits are encoded by two genes, Glu-1Bx14 and Glu-1Bx15, which are tightly linked and located on the 1BL. Protein assay by SDS-PAGE indicated that the expression of Glu-1Bx14 gene was always much stronger than that of Glu-1By15 in the same variety. But, variation of expression level for Glu-1By15 gene existed among varieties, such as in Xiaoyan 54, Xiaoyan 6, Yanzhan 1 and Shanyou 225. We also investigated the transcription difference of Glu-1By14 and Glu-1By15 genes in Xiaoyan 54 and Shanyou 225 by semi-quantitative RT-PCR method. The Glu-1By14 always transcripts much more than the Glu-1By15. This was basically consistent with the translation difference between the two genes. Promoters of 1Bx14, 1By15, 1By8, 1Dx2 and 1Dy12 were cloned from Xiaoyan 54, Chinese Spring and Aegilops tauschii. Sequence analysis indicated that the HMW-GS genes had high homology at their promoter regions. However, significant difference existed between sequence of 1Bx14 promoter and those of other HMW-GS genes. The transient expression experiment showed that the promoter of 1By15 has lower activity than that of 1Bx14, which was consistent with their transcription level of the two genes in varieties. In addition, transient expression of the gus driven by the promoter （P2） of HMW-GS 1Dx2 gene was higher than by other HMW-GS promoters. Therefore, we constructed 1By15 gene expression vector driven by the 1Dx2 promoter, and transformed the 1By15 gene into wheat commercial variety, Jimai 20 by pollen tube method. Of 45 independent transgenic lines identified by PCR, 3 were confirmed to contain the HMW-GS 1By15 gene via Southern hybridization. The delivered 1B

关键词： HMW-GS 1By15 gene transient expression assay transgenic

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Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone

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Chinese Chemical Letters 2007年第12期18卷 1490-1492页

作者： yu dong shen yu Wang Shi Wei Zhang Zhi LiXiao yuan Ming Sun Xian Zhang Bu Lian Quan Gu Food Quality and Safety Key Laboratory of Department of Education of Guangdong Province/Institute of Food Quality and Safety South China Agricultural University Guangzhou 510642 China School of Pharmaceutical Sciences Sun Yat-Sen University Guangzhou 510080 China

A good strategy was brought forward for designing efficient haptens and complete antigens for 3-amino-2-oxazolidinone （AOZ）. Haptens designed newly were achieved facilely in good yield by using LiCI-N（Et）3 as new catalysis system, the structure of which was elucidated by spectroscopy analysis, such as NMR and MS. Novel antigens for AOZ were prepared successfully by convenient active ester method. The ratios of haptens 3 and 4 to carrier proteins were proven respectively as 41 ： 1 （5a）, 39：1 （6a）, 11：1 （5b） and 9：1 （6b） by trinitrobenzene sulfonic acid （TNBS） method. The results of indirect competitive ELISA （ic-ELISA） of antiserums indicated that the haptens with a short unsaturated side chain can evoke specific immune response effectively.

关键词： Furazolidone AOZ Antigen Hapten Immunoassay

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Reveal short range interactions between u/d quarks in the NN, D03, and D30 systems

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Science China(Physics,Mechanics & Astronomy) 2025年第3期Mechanics & Astronomy) .卷 106-115页

作者： Qi-Fang Lü yu-Bing dong Peng-Nian shen Zong-Ye Zhang Department of Physics Hunan Normal University Key Laboratory of Low-Dimensional Quantum Structures and Quantum Control of Ministry of Education Key Laboratory for Matter Microstructure and Function of Hunan Province Hunan Normal University Institute of High Energy Physics Chinese Academy of Sciences School of Physical Sciences University of Chinese Academy of Sciences

The dynamic mechanism of short range interactions between u/d quarks remains an open and challenging problem. In order to reveal this quark dynamics, we perform a systematic analysis of N N, D03, and D30systems in the（extended） chiral SU（3） constituent quark models. By comparing results calculated with different models and different parameter sets, the effects of one gluon exchange and vector meson exchange terms are carefully examined. The results indicate that the vector meson exchange interactions dominate the short range interactions between u/d quarks, whereas the small residual one gluon exchange coupling strength is also allowed.

关键词： short range interactions dibaryons constituent quark models

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Unexpected Synthesis of 2,2'-[1-(4-Methoxyphenoxy)propane-2,2-diyl]bis(5-methoxy-3-methylbenzofuran)

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Chinese Chemical Letters 2005年第12期16卷 1581-1583页

作者： yu dong shen Hai Qiang WU Lin Kun AN Zhi Shu HUANG Xian Zhang BU Lian Quan GU School of Chemistry and Chemical Engineering Sun Yat-sen University Guangzhou 510275 School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510080

A new bisbenzofuran analogue Ⅶ was achieved unexpectedly in one step procedure from 1-（4-methoxyphenoxy）acetone Ⅰ by using Amberlyst 15 resin as catalyst in excellent yield. The structure was elucidated by spectro... 详细信息

关键词： Benzofuran Amberlyst 15 resin cyclization

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Detection of Perfect Stealthy Attacks on Cyber-Physical Systems Subject to Measurement Quantizations: A Watermark-Based Strategy

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IEEE/CAA Journal of Automatica Sinica 2025年第1期12卷 114-125页

作者： yu-Ang Wang Zidong Wang Lei Zou Bo shen Hongli dong the College of Information Science and Technology Donghua University the Engineering Research Center of Digitalized Textile and Fashion Technology Ministry of Education IEEE the College of Electrical Engineering and Automation Shandong University of Science and Technology the Department of Computer Science Brunel University London Uxbridge the Sanya Offshore Oil and Gas Research Institute Northeast Petroleum University the Artificial Intelligence Energy Research Institute Northeast Petroleum University Heilongjiang Provincial Key Laboratory of Networking and Intelligent Control Northeast Petroleum University

In this paper, the attack detection problem is investigated for a class of closed-loop systems subjected to unknownbutbounded noises in the presence of stealthy attacks. The measurement outputs from the sensors are quantized before transmission.A specific type of perfect stealthy attack, which meets certain rather stringent conditions, is taken into account. Such attacks could be injected by adversaries into both the sensor-toestimator and controller-to-actuator channels, with the aim of disrupting the normal data flow. For the purpose of defending against these perfect stealthy attacks, a novel scheme based on watermarks is developed. This scheme includes the injection of watermarks(applied to data prior to quantization) and the recovery of data(implemented before the data reaches the estimator).The watermark-based scheme is designed to be both timevarying and hidden from adversaries through incorporating a time-varying and bounded watermark signal. Subsequently, a watermark-based attack detection strategy is proposed which thoroughly considers the characteristics of perfect stealthy attacks,thereby ensuring that an alarm is activated upon the occurrence of such attacks. An example is provided to demonstrate the efficacy of the proposed mechanism for detecting attacks.

关键词： Attack detection cyber-physical systems (CPSs) perfect stealthy attacks watermark-based strategy

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Dispersion of Soluble Matters in Newton-dipolar Stratified Fluid and Effects of Peripheral Layer

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Communications in Nonlinear Science and Numerical Simulation 1998年第4期3卷 236-241页

作者： Zhangji LU, Shousheng dong, yu shen and Guangping ZHENG (Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433,China)(Department of Physics, East China Normal University, Shanghai 200062, China) Dept. of Mechanics and Eng. Science Fudan University Shanghai 200433 China Department of Physics East China Normal University Shanghai 200062 China

In the paper, the dispersion law and the concentration distributions of soluble matters in Newton-dipolar fluids flowing through a circular tube have been investigated. Main results are: (1) for the dependence of M on A (or H), the completely opposite trends are obtained in the cases with and without the peripheral layer. (2) effects of δ on　M have the minimum values near 8=0.85-0.9. (3) various models such as couple stress,micropolar, dipolar, Newton-newtonian, Newton-couple stress and Newton-micropolar　model etc. are all special cases of Newton-dipolar fluid (where Mz=0). WhenMz≠ 0,however, there are evident differences between the Newton-dipolar fluid and the Newtoncouple stress fluid, the Newton-micropolar fluid.

关键词： dipolar fluid dispersion effects of a peripheral layer tube flow of complex fluid

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Evaluating complementaries among urban water,energy,infrastructure,and social Sustainable Development Goals in China

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Journal of Environmental Sciences 2025年第3期149卷 585-597页

作者： Ke yu Yifan Song Jin Lin shen Qu School of Management and Economics Beijing Institute of TechnologyBeijing 100081China Center for Energy&Environmental Policy Research Beijing Institute of TechnologyBeijing 100081China

Urban areas’performance in water,energy,infrastructure,and socio-economic sectors is intertwined and measurable through Sustainable Development Goals(SDGs)6–*** synergy among these is critical for *** study constructs an indicator framework that reflects progress towards these urban SDGs in *** indicate underperformance in SDGs 8–11,suggesting the need for transformative *** network analysis,the research reveals complementarities among these ***,the SDG space divides into socio-economic and ecological clusters,with SDG 6(Clean Water and Sanitation)central to ***,SDG 8(Decent Work and Economic Growth)and SDG 9(Industry,Innovation,and Infrastructure)act as bridges,while greater synergies exist between SDG 12(Responsible Consumption and Production)and SDG 13(Climate Action).An in-depth view at the indicator-level shows a core-periphery structure,emphasizing indicators like SDG 6.2(Wastewater Treatment Rate)and SDG 6.6(Recycled Water Production Capacity per capita)as *** study confirms the urban SDG space’s stability and predictiveness,underscoring its value in steering well-aligned policy decisions for sustainable growth.

关键词： Sustainable development goals Synergy Urban development Policy decisions

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Permutation orbifolds of vertex operator superalgebras and associative algebras

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Science China Mathematics 2025年第1期68卷 19-38页

作者： Chongying dong Feng Xu Nina yu Department of Mathematics University of California at Santa CruzSanta CruzCA 95064USA Department of Mathematics Huzhou UniversityHuzhou 313000 China Department of Mathematics University of California at RiversideRiversideCA 92521USA School of Mathematical Sciences Xiamen UniversityXiamen 361005China

Let V be a vertex operator superalgebra and g=(12···k)be a k-cycle which is viewed as an automorphism of the tensor product vertex operator superalgebra V^(■k).In this paper,we construct an explicit isomorphism from A_(g)(V^(■k))to A(V)if k is odd and to Aσ(V)if k is even whereσis the canonical automorphism of V of order 2 determined by the superspace structure of *** recover previous results by Barron(2016)and Barron and Vander Werf(2014)that there is a one-to-one correspondence between irreducible g-twisted V^(■k)-modules and irreducible V-modules(***σ-twisted V-modules)when k is odd(***).This explicit isomorphism is expected to be useful in our further study on the Zhu algebra of the fixed point subalgebra.

关键词： permutation orbifold vertex operator superalgebra Zhu algebra

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Hitchhiking Effect Mapping: A New Approach for Discovering Agronomic Important Genes

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Agricultural Sciences in China 2007年第3期6卷 255-264页

作者： ZHANG Xue-yong TONG Yi-ping YOU Guang-xia HAO Chen-yang GE Hong-mei WANG Lan-fen LI Bin dong yu-shen LI Zhen-sheng Institute of Crop Sciences Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Germplasm ＆ Biotechnology Ministry of Agriculture/National Facility for Crop Gene Resources and Genetic Improvement Beijing 100081 P.R.China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 P.R.China

Besides the natural selection, the crops cultivated today have experienced two episodes of strong artificial selection, domestic and modern breeding. Domestication led to giant genetic structure differentiation between cultivars and their wild species, while modern breeding made further genetic structure differentiation between the modern varieties and the landraces. In a population, diversity of the loci under strong selection is significantly lower than that of other loci. At the same time, diversity in the genomic regions flanking these selected loci also declines in the process of selection. This phenomenon is called hitchhiking effects or selection sweep in genetics. Genomic regions with selection sweep （haplotype block） could be detected after draft genome scanning （genome typing） with molecular markers in a number of released varieties or natural populations. Marker/trait association analysis in these regions would detect the loci （or QTLs） even the favored alleles （genes） in breeding or natural adaptation. Fine scanning of these genomic regions would help to determine the sizes of haplotype blocks and to discover the key genes, thereby providing very valuable information for isolation of the key genes and molecular design of new varieties. Establishment of high density genetic linkage maps in the major crops and availability of high throughput genotyping platform make it possible to discover agronomic important genes through marker/trait association analysis. On the basis of available publications, we give a brief introduction of the hitchhiking effect mapping approach in this paper using plant height, 1 000-grain weight, and phosphorus-deficiency tolerance as examples in wheat.

关键词： domestication & breeding hitchhiking effects marker/trait association analysis gene discovery

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Numerical simulation of microstructure and microporosity morphology in directional solidification of aluminum-copper alloys:Effect of copper content and withdrawal rate

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China Foundry 2025年第1期22卷 33-44页

作者： Wei yuan Hai-dong Zhao Xu shen Chun Zou yuan Liu Qing-yan Xu National Engineering Research Center of Near-Net-Shape Forming for Metallic Materials South China University of TechnologyGuangzhou 510640China Key Laboratory for Advanced Materials Processing Technology(Ministry of Education) Tsinghua UniversityBeijing 100084China

Microporosity formed in the solidification process of Al alloys is detrimental to the alloy properties.A two-dimensional cellular automaton(CA)model was developed to simulate the microstructure and microporosity formation in Al-Cu alloys,considering variations in Cu content and solidification *** results indicate that the Cu content primarily influences the growth of *** validate the model,directional solidification experiments were conducted on Al-Cu alloys with varing Cu contents and withdrawal *** experimental results of dendrites and microporosity characteristics agree well with the predictions from the developed model,thus confirming the validity of the *** alloy’s liquidus temperature,dendrite morphology,and hydrogen saturation solubility arising from different Cu contents have significant effects on microporosity *** withdrawal rate primarily affects the nucleation of hydrogen microporosity by altering cooling rates and dendritic growth rates,resulting in different microporosity characteristics.

关键词： microporosity dendrites cellular automaton Al-Cu alloys directional solidification

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