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Helicobacter pylori and 17β-estradiol induce human intrahepatic biliary epithelial cell abnormal proliferation and oxidative DNA damage

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Hepatobiliary & Pancreatic Diseases International 2017年第5期16卷 519-527页

作者： Fei Ma yong yang Jian-dong Wang Zhi-Wei Quan Di Zhou Department of Oncology Xinhua Hospital Shanghai Jiaotong University School of Medicine Department of General Surgery Shanghai Jiaotong University School of Medicine

BACKGROUND: Biliary cancers are more common in females, and previous studies have suggested that Helicobacter pylori (H. pylori) exists in the biliary system. However, the effects of H. pylori infection and estrogen on the biological behaviors of human biliary epithelium mucosa remain unknown. The present study aimed to clarify their effects on the proliferation, apoptosis, migration and oxidative DNA damage of a human intrahepatic biliary epithelial cell (HIBEC) line in vitro. METHODS: HIBECs were co -cultured with 17 beta-estradiol (at 10(-9) mol/L, 10(-7) mol/L, and 10(-5) mol/L) and H. pylori (at MOI=0.5:1, 1:1, and 2:1) and continuously passaged until the 15th generation (approximately 45 days). Then, the following assays were performed. HIBEC proliferation was measured using the CCK-8 assay, plate clone-formation assay and by determining Ki-67 expression with immunocytochemistry; cell apoptosis and migration were investigated using Annexin-V/PI and transwell assays, respectively; and reactive oxygen species (ROS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) production were detected by flow cytometry and immunofluorescence staining combined with confocal laser scanning microscopy, respectively. The results were the basis for evaluating the level of oxidative stress and the related DNA damage in HIBECs. RESULTS: HIBECs maintained a normal morphology and vitality when treated with 17 beta-estradiol (at 10(-9) mol/L) and H. pylori (at MOI=0.5:1 and 1:1). 17 beta-estradiol at 10(-7) mol/L and 10(-5) mol/L and H. pylori at MOI=2:1, by contrast, caused cell death. Compared with controls, HIBECs treated with 17 beta-estradiol (10(-9) mol/L) and H. pylori (MOI=1:1) had a higher up-regulation of proliferation, Ki-67 expression, clone formation, migration activity and the expression of ROS and 8-OHdG and exhibited a down-regulation of apoptosis. The above effects were further increased when 17 beta-estradiol and H. pylori were combined (P<0.05). CONCLUSIONS: H. pylori and 17

关键词： H. pylori 17 beta-estradiol binary tract proliferation

Postoperative infection of the skull base surgical site due to suppurative parotitis:A case report

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World Journal of Clinical Cases 2022年第15期10卷 4991-4997页

作者： yong Zhao yang Zhao Li-Qin Zhang Guo-dong Feng Department of Otorhinolaryngology-Head and Neck Surgery Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

BACKGROUND Paraganglioma occurring at the lateral skull base is a rare *** is the primary treatment of benign *** infection of the surgical site at the lateral skull base is very dangerous and hard to *** SUMMARY A 30-year-old man with a 1-year history of left-side progressive hearing loss,tinnitus,facial palsy,and choking failed conventional treatment and is the focus of this case *** revealed a mass around the left jugular foramen that was approximately 47 mm×38 mm×34 mm in size and had eroded the bone of the vertebral and horizontal segments of the internal carotid *** tumor breached the meninges and occupied the cerebella pontine region.A two-stage surgery was designed for the resection of the *** the first-stage,the epidural portion of the mass was *** abdominal fat and the temporal muscle flap were transposed within the surgical *** surgery was successful;however,25 d after surgery,he developed suppurative parotitis,and the infection spread to the surgical site at the skull ***-spectrum antibiotics were used,and debridement was *** that,the wound was cleaned *** months after the first-stage surgery,the wound was still unclosed,and there was intermittent purulent exudation within the surgical *** sealing drainage(VSD)was used,and the wound healed in a *** year after the first surgery,the second-stage of the operation was performed to remove the intracranial portion of the *** of the tumor was not detected after a 6-month *** After a lateral skull base surgery,suppurative parotitis can spread into the operative cavity leading to infection of the surgical *** can help to effectively heal the infected wound.A two-stage surgical approach offers a safer option for removing the lateral skull base paraganglioma that involves the meninges.

关键词： Postoperative infection Lateral skull base Suppurative parotitis Vacuum sealing drainage Two-stage surgery Case report

STUDY OF DYE-SENSITIZED SYSTEMS FOR PHOTOIMAGING

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Chinese Journal of Polymer Science 2001年第1期19卷 79-83页

作者： Fang Gao Chun-ying Zhao yong-yuan yang Li-dong Li Insatute of Photographic Chemistry Chinese Academy of Sciences Beijing 100101 China Insatute of Photographic Chemistry Chinese Academy of Sciences Beijing 100101 China Insatute of Photographic Chemistry Chinese Academy of Sciences Beijing 100101 China Insatute of Photographic Chemistry Chinese Academy of Sciences Beijing 100101 China

o-Chloro-hexaarylbiimidazole (o-CI-HABI) can be sensitized efficiently by cyanine dyes, the cyclopentanone and cyclohexanone dyes, when exposed to xenon lamp (use filter cut lambda less than or equal to 400 nm). The photoreaction between the photoinitiator and the dyes was completed through an electron transfer process. Excellent results have been obtained in photoimaging studies, e.g. the resolution of the image can reach 7 mum. The influence of the content of the dyes and the heat after the exposure on the resolution of the image was investigated.

关键词： photopolymer photoimaging hexaarylbiimidazole printing plate

Individualized nomogram improves diagnostic accuracy of stage Ⅰ-Ⅱ gallbladder cancer in chronic cholecystitis patients with gallbladder wall thickening

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Hepatobiliary & Pancreatic Diseases International 2016年第2期15卷 180-188页

作者： Di Zhou Jian-dong Wang yong yang Wen-Long Yu yong-Jie Zhang Zhi-Wei Quan Department of General Surgery Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092 China Department of Biliary Tract Surgery Ⅱ Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

BACKGROUND： Early diagnosis of gallbladder cancer（GBC） can remarkably improve the prognosis of patients. This study aimed to develop a nomogram for individualized diagnosis of stage Ⅰ-Ⅱ GBC in chronic cholecystitis patients with gallbladder wall ***： The nomogram was developed using logistic regression analyses based on a retrospective cohort consisting of 89 consecutive patients with stage Ⅰ-Ⅱ GBC and 1240 patients with gallbladder wall thickening treated at one biliary surgery center in Shanghai between January 2009 and December 2011. The accuracy of the nomogram was validated by discrimination, calibration and a prospective cohort treated at another center between January 2012 and December 2014（n=928）.RESULTS： Factors included in the nomogram were advanced age, hazardous alcohol consumption, long-standing diagnosed gallstones, atrophic gallbladder, gallbladder wall calcification, intraluminal polypoid lesion, higher wall thickness ratio and mucosal line disruption. The nomogram had concordance indices of 0.889 and 0.856 for the two cohorts, respectively. Internal and external calibration curves fitted well. The area under the receiver-operating characteristic curves of the nomogram was higher than that of multidetector row computed tomography in diagnosis of stage Ⅰ-Ⅱ GBC（P〈0.001）.CONCLUSION： The proposed nomogram improves individualized diagnosis of stage Ⅰ-Ⅱ GBC in chronic cholecystitis patients with gallbladder wall thickening, especially for those the imaging features alone do not allow to confirm the diagnosis.

关键词： chronic cholecystitis gallbladder wall thickening gallbladder cancer individualized diagnosis nomogram

iTRAQ-based quantitative proteome characterization of wheat grains during filling stages

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Journal of Integrative Agriculture 2017年第10期16卷 2156-2167页

作者： CUI yong yang Ming-ming dong Jian ZHAO Wan-chun GAO Xiang College of Agronomy Northwest A&F University Yangling 712100 P.R.China Wheat Engineering Research Center of Shaanxi Province Yangling 712100 P.R. China

Using isobaric tags for relative and absolute quantification （iTRAQ） and associated analytic technologies, we have cataloged and compared 7 069 unique wheat proteins expressed during four substages of the filling stage. Among them, 859 are differentially expressed, showing at least a 2-fold difference in concentration across substages. Differentially expressed proteins （DEPs） includind high-molecular weight giutenin subunit （W5AIU1）, low-molecular weight glutenin subunit （QSW3V4）, gliadin/avenin-like seed protein （D2KFG9）, and avenin-like protein （W5DVL2）, all of which have previously been identified as important for nutritional quality and bread-making properties, and all of which were found to increase at the latter stages of development. We have applied statistical techniques to group the proteins into hierarchical clusters, and have consulted databases to infer functional and other relationships among the identified proteins.

关键词： wheat proteome isobaric tags for relative and absolute quantification (iTRAQ) grain filling

Synthesis and mesomorphic properties of novel tolane-type liquid crystals

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Chinese Chemical Letters 2009年第11期20卷 1283-1286页

作者： dong Yu Zhao Qing yong Meng Xiao Peng Cui Huai yang Department of Materials Physics and Chemistry School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

Two series of novel tolane-type liquid crystals （LCs） comprising of hydrogen-bonded organic acids were synthesized. The formation of dimerized H-bond LCs was confirmed by IR spectroscopy, and mesomorphic properties of the LCs were investigated by differential scanning calorimetry （DSC） and polarizing optical microscopy （POM）. It was found that the end groups of the liquid crystals as well as the unsaturated rigid core had effect on the mesomorphic properties.

关键词： Tolane Hydrogen-bonded liquid crystal Mesomorphic properties Synthesis

Numerical study on wave loads and motions of two ships advancing in waves by using three-dimensional translating-pulsating source

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Acta Mechanica Sinica 2013年第4期29卷 494-502页

作者： yong Xu Wen-Cai dong Department of Naval Architecture Naval University of Engineering

A frequency domain analysis method based on the three-dimensional translating-pulsating （3DTP） source Green function is developed to investigate wave loads and free motions of two ships advancing on parallel course in waves. Two experiments are carried out respectively to mea- sure the wave loads and the free motions for a pair of side-by- side arranged ship models advancing with an identical speed in head regular waves. For comparison, each model is also tested alone. Predictions obtained by the present solution are found in favorable agreement with the model tests and are more accurate than the traditional method based on the three dimensional pulsating （3DP） source Green function. Numer- ical resonances and peak shift can be found in the 3DP pre- dictions, which result from the wave energy trapped in the gap between two ships and the extremely inhomogeneous wave load distribution on each hull. However, they can be eliminated by 3DTP, in which the speed affects the free sur- face and most of the wave energy can be escaped from the gap. Both the experiment and the present prediction show that hydrodynamic interaction effects on wave loads and free motions are significant. The present solver may serve as a validated tool to predict wave loads and motions of two ves- sels under replenishment at sea, and may help to evaluate the hydrodynamic interaction effects on the ships safety in replenishment operation.

关键词： Hydrodynamic interaction - Wave loads ~Ship motions ~ Model test ~ Three-dimensional translating-pulsating source ~ Underway replenishment

Third generation high strength low alloy steels with improved toughness

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Science China(Technological Sciences) 2012年第7期55卷 1797-1805页

作者： SUN XinJun LI Zhaodong yong QiLong yang ZhiGang dong Han WENG YuQing Institute for Structural Materials Central Iron and Steel Research InstituteBeijing 100081China Department of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Chinese Society for Metals Beijing 100711China

On-line thermo mechanical controlled processing(TMCP) was conducted to develop the third generation high strength low alloy(HSLA) steel with high toughness *** ultra-low carbon content ensured a high level of upper shelf energy while ultrafine lath martensitic structure transformed from super-thin pancaked austenite during controlled rolling and *** reduction of martensite block size decreased ductile-to-brittle transition temperature(DBTT) and compensated the strength loss due to carbon ***,the excellent balance of strength and toughness values was obtained as 950-1060 MPa for yield strength,180 J for Charpy V-notch impact absorbed energy at 30℃,which is much superior to that of traditional martensitic *** mechanisms for the refinement of lath martensite block were proposed:One is the austenite grain refinement in the direction of thickness,and the other is the reduction in the fraction of sub-block boundaries with small misorientation and the increase in the fraction of block boundaries with large misorientation,possibly due to austenite hardening.

关键词： pancaked austenite lath martensite precipitate TMCP HSLA steel

Electro-Hydraulic Servo Control System Based on a Novel Generic Model Control Method

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Journal of Iron and Steel Research International 2010年第10期17卷 23-27页

作者： DAI yong-bin yang Wei-dong WANG Shao-fu ZHANG Ming College of Software Liaoning University of Technology Jinzhou 121001 Liaoning China College of Information Engineering University of Science and Technology Beijing Beijing 100083 China

A novel electro-hydraulic servo control algorithm was introduced. The primary idea of this algorithm was to combine the notion of model prediction with the generic model control （GMC）. The performance of the GMC al- gorithm was improved by receding optimization and feedback correction. By the predictive model of output errors of an electro-hydraulic servo control system based on the improved weighted least square support vector machines （WLS-SVM） to forecast and compensate the future sequence errors, the control precision and robustness were im- proved. The improved GMC based on the improved WLS-SVM was applied to an electro-hydraulic servo control sys- tem for hot strip rolling control systems. The simulation results showed the feasibility and effectiveness of the pres- ent algorithm.

关键词： predictive control improved WLS-SVM electro-hydraulic servo control system