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Sensory Phenotypic and Molecular Identification of Aromatic Rice Accessions Cultivated in Benin

Advances in Bioscience and Biotechnology 2024年第3期15卷 195-206页

作者： Chimène Nadège Mahoussi Nanoukon Koffi David Montcho Hambada Deless Edmond Fulgence Thiémélé Bignon Meyrix Pamela Franzel Loumedjinon Babatoundé Franel Carel Wenceslas Affolabi Amed Sèmèvo Havivi Kéllya Laurinzo Déguénon Grâce Finagnon Vitoekpon Arielle Bankole Lambert gustave djedatin Laboratory of Molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Bio-technologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin Laboratory of Molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Biotechnologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin Laboratory of Plant Horticultural and Forestry Sciences National University of Agriculture Kétou Benin Genetics Teaching and Research Unit Department of Biochemistry-Genetics Peleforo GON COULIBALY University Korhogo C&#244te d’Ivoire

Rice is one of the most widely cultivated cereals in the world, and its aroma is increasingly in demand. With the advancement of research, a major rice flavor gene has been identified on rice chromosome 8. It encodes non-functional betaine aldehyde dehydrogenase leading to the accumulation of 2-acetyl-1-pyrroline which is the major olfactory compound that confers the fragrant character to rice. The aroma of rice is considered a special trait of enormous economic importance that determines the prime price in world trade. To satisfy the needs of the population and reduce rice imports into Benin, we conducted this study to identify aromatic rice accessions grown in Benin. Seventy-two rice accessions collected across Benin were PCR amplified with three SSR markers RM 7049, Aro 7, and RM 223, linked to the fgr (fragrance of rice) aroma gene. Molecular analysis revealed that 12 of the 72 accessions, namely Bagou 19, Bagou 22, Tchaka 34, Foun 15, Tchaka 41, Nana 32, Kan 61, Kung 69, Kung 67, Bagou 20, Agbab 101 and Koum 55 possess the fgr gene and can be considered as aromatic rice accessions. A sensory phenotypic test using KOH was carried out on rice accessions carrying fgr gene. Of the twelve positives, only one had the smell of aromatic rice, like the Azucena control. These results show that Benin also has aromatic rice varieties that can be sold on national and international markets.

关键词： Aromatic Rice 2-Acetyl-1-Pyrroline SSR Markers Benin

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Sensory Phenotypic and Molecular Identification of Aromatic Rice Accessions Cultivated in Benin

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Advances in Bioscience and Biotechnology（生命科学与技术进展） 2024年第3期 195页

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Insight into Genetic Diversity of Cultivated Lima Bean (Phaseolus lunatus L.) in Benin

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American Journal of Molecular Biology 2023年第1期13卷 32-45页

作者： Rose Fernande Fagbédji Lambert gustave djedatin Chimène Nanoukon Geofroy Kinhoegbe Amed Havivi Hounnankpon Yédomonhan Clément Agbangla Laboratoire de Biologie Moléculaire et de Bioinformatique Appliquée à la Génomique (BIOGENOM) ENSBBA de Dassa-Zoumé Université Nationale des Sciences Technologies Ingénierie et Mathématiques (UNSTIM) Abomey Benin Ecole Normale Supérieure de l’Enseignement Technique (ENSET) de Lokossa Université Nationale des Sciences Technologies Ingénierie et Mathématiques (UNSTIM) Abomey Benin Laboratoire de Botanique et Ecologie Végétale Faculté des Sciences et Techniques (FAST) Université d’Abomey-Calavi (UAC) Abomey-Calavi Benin Département de Génétique et des Biotechnologies Faculté des Sciences et Techniques (FAST) Université d’Abomey-Calavi Abomey-Calavi Benin

Lima bean is a tropical and subtropical legume from the genus Phaseolus which is cultivated for its importance in food and in medicine, but which remains a Neglected and Underutilized Crop in Benin. Understanding the genetic diversity of a species’ genetic resources is useful for the establishment of appropriate conservation strategies and breeding programs and for sustainable use. We use 6 out of ten SSR markers to analyze the diversity and population structure of 28 Lima bean landraces collected in Benin. A total of 28 alleles with an average of 4.16 alleles per SSR were amplified. The Polymorphic Information Content value ranged from 0.079 to 0.680 with an average of 0.408. The analysis of population structure revealed three subpopulations. PCoA revealed three well-separated clusters among the analyzed accessions in accordance with the population structure results and the clustering based on the Neighbor-Joining tree. AMOVA showed highly significant (p = 0.001) diversity among and within populations. Hence, 32% of the genetic variation was distributed among the population and 68% was distributed within populations. A high PhiP value (0.321) was found between the three sub-subpopulations indicating a high genetic differentiation between these sub-subpopulations. By exhibiting the highest average number of alleles, Shannon-Weaver information and Shannon-Weaver diversity indices, and the highest mean number of private alleles, subpopulation 1 is the main gene pool of the analyzed collection. The present study is an important starting point for the establishment of appropriate conservation strategies and breeding programs for Lima bean genetic resources.

关键词： Phaseolus lunatus L. Benin Genetic Diversity SSR Markets Conservation

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Modification of the Genetic Polymorphism of Glutathione-S-Transferase (GSTM1 and GSTT1) in Motorcycle Drivers Exposed to BTEX in Cotonou

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Journal of Biosciences and Medicines 2022年第11期10卷 104-112页

作者： Cachon Fresnel Boris Sagbo H. Firmin Nanoukon Chimène djedatin gustave Atindehou Ménonvè Senou Maximin Ayi-Fanou Lucie Laboratory of Biochemistry Molecular Biology and Environment Faculty of Sciences and Techniques University of Abomey-Calavi Cotonou Benin Laboratory of Experimental and Clinical Biology National School of Applied Biosciences and Biotechnologies National University of Sciences Technologies Engineering and Mathematics Abomey Benin Laboratory of Molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Biotechnologies National University of Sciences Technologies Engineering and Mathematics Abomey Benin

The glutathione-S-transferase genes mainly the GSTM1 and GSTT1 alleles are responsible for the synthesis of detoxication enzymes that can remove toxic substances. The objective of this study is to seek changes in the genetic polymorphism of glutathione-S-transferase GSTM1 and GSTT1 in motorcycle drivers exposed to BTEX. Our study group consists of 60 motorcycle drivers including 30 professional and 30 non-professional. Blood samples were preleveled from the study population in the EDTA tubes and DNA was extracted by the phenol/chloroform method. The PCR technique was used to determine the presence or absence of genes. Our results showed that the percentage of GSTM1 null genotype has a statistically significant difference (P = 0.02), while the percentage of GSTT1 null genotype was non-significant (P = 0.76) between the two groups. The percentage of deletion of both genes is higher in professional than non-professional motorcycle drivers. Air pollution in Cotonou by BTEX seems to influence the deletion of the GSTM1 and GSTT1 genes at a higher percentage among professional than non-professional motorcycle drivers.

关键词： BTEX Glutathione-S-Transferase Deletion PCR Motorcycle Drivers

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Insight into Genetic Diversity of Cultivated Lima Bean (Phaseolus lunatus L.) in Benin

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American Journal of Molecular Biology（美国分子生物学期刊） 2023年第1期

作者： Rose Fernande Fagbédji Lambert gustave djedatin Chimène Nanoukon Geofroy Kinhoegbe Amed Havivi Hounnankpon Yédomonhan Clément Agbangla

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