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Layout design of a mixed-flow production line based on processing energy consumption and buffer configuration

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Advances in Manufacturing 2021年第3期9卷 369-387页

作者： Cai-xia Zhang Shu-Lin Dong Hong-yan Chu Guo-Zhi ding Zhi-Feng liu Shi-Yao Guo Chong-bin yang Institute of Advanced Manufacturing and Intelligent Technology Beijing University of TechnologyBeijing100124People’s Republic of China Beijing Key Laboratory of Advanced Manufacturing Technology Beijing University of TechnologyBeijing100124People’s Republic of China Beijing Xinghang Electromechanical Equipment Co. Ltd.Beijing100074People’s Republic of China School of Mechanical Engineering and Automation Beihang UniversityBeijing100191People’s Republic of China

Green manufacturing is a growing trend,and an effective layout design method for production lines can reduce resource wastage in *** study focuses on existing problems such as low equipment utilization,long standby time,and low logistics efficiency in a mixed-flow parallel production *** reduce the energy consumption,a novel method considering an independent buffer configuration and idle energy consumption analysis is proposed for this production line’s layout design.A logistics intensity model and a machine tool availability model are established to investigate the influences of independent buffer area configuration on the logistics intensity and machine tool *** solve the coupling problem between machine tools in such production lines,a decoupling strategy for the relationship between machine tool processing rates is *** energy consumption model for the machine tools,based on an optimized configuration of independent buffers,is *** model can effectively reduce the idle energy consumption of the machine tools while designing the workshop ***,considering the problems encountered in workshop production,a comprehensive optimization model for the mixed-flow production line is *** verify the effectiveness of the mathematical model,it is applied to an aviation cabin production *** results indicate that it can effectively solve the layout problem of mixed-flow parallel production lines and reduce the idle energy consumption of machine tools during *** proposed buffer configuration and layout design method can serve as a theoretical and practical reference for the layout design of mixed-flow parallel production lines.

关键词： Layout design Energy consumption Mixed flow production line Independent buffer configuration

Untreated Prior Pulmonary Tuberculosis Adversely Affects Pregnancy Outcomes in Infertile Women Undergoing in vitro Fertilization and Embryo Transfer: A Large Retrospective Cohort Study

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Biomedical and Environmental Sciences 2021年第2期34卷 130-138页

作者： GAI xiao yan CHI Hong bin ZENG Lin CAO Wen Li CHEN Li Xue ZHANG Chen LU Ming NING Lan ding CHANG Chun ZHANG Wei xia liu Ping LI rong SUN Yong Chang QIAO Jie Department of Respiratory and Critical Care Medicine Peking University Third HospitalBeijing 100191China Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Research Center of Clinical Epidemiology Peking University Third HospitalBeijing 100191China Tuberculosis Department Beijing Geriatric HospitalBeijing 102699China Information Center Peking University Third HospitalBeijing 100191China

Objective Prior pulmonary tuberculosis(PTB) on chest X-ray(CXR) was commonly found in infertile patients receiving examinations before in vitro fertilization and embryo transfer(IVF-ET). It was unclear whether untreated PTB would affect pregnancy outcomes after *** We conducted a retrospective cohort study of 14,254 infertile patients who had received IVFET at Peking University Third Hospital in 2017. Prior PTB was defined as the presence of signs suggestive of old or inactive PTB on CXR, with or without a clinical TB history. Patients who had prior PTB on CXR but had not received a clinical diagnosis and anti-TB therapy were included for analysis. Live birth,clinical pregnancy, and miscarriage rates were compared between the untreated PTB and non-PTB *** The untreated PTB group had significantly lower clinical pregnancy(31.7% vs. 38.1%) and live birth(23.8% vs. 30.6%) rates than the non-PTB group(both P < 0.001). Multivariate analysis revealed that untreated PTB was a risk factor for decreased live birth rate [odds ratio(OR), 0.80;95% confidence interval(CI), 0.66–0.98;P = 0.028] in all patients and for increased miscarriage(OR, 4.19;95% CI,1.69–10.39;P = 0.002) and decreased live birth(OR, 0.45;95% CI, 0.24–0.83;P = 0.011) rates in patients with unexplained *** Untreated PTB was associated with adverse pregnancy outcomes after IVF-ET, especially in patients with unexplained infertility, highlighting the clinical significance of PTB in this specific patient population.

关键词： Tuberculosis Embryo transfer In vitro fertilization Infertility Pregnancy outcome

Overexpression of the Suaeda salsa SsNHX1 gene confers enhanced salt and drought tolerance to transgenic Zea mays

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Journal of Integrative Agriculture 2018年第12期17卷 2612-2623页

作者： HUANG Ying ZHANG xiao-xia LI Yi-hong ding Jian-zhou DU Han-mei ZHAO Zhuo ZHOU Li-na liu Chan GAO Shi-bin CAO Mo-ju LU yan-li ZHANG Su-zhi Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of Agriculture/Maize Research Institute Sichuan Agricultural University

Maize is one of the most important crops worldwide, but it suffers from salt stress when grown in saline-alkaline soil. There is therefore an urgent need to improve maize salt tolerance and crop yield. In this study, the SsNHX1 gene of Suaeda salsa, which encodes a vacuolar membrane Na~+/H~+ antiporter, was transformed into the maize inbred line 18-599 by Agrobacterium-mediated transformation. Transgenic maize plants overexpressing the SsNHX1 gene showed less growth retardation when treated with an increasing NaCl gradient of up to 1%, indicating enhanced salt tolerance. The improved salt tolerance of transgenic plants was also demonstrated by a significantly elevated seed germination rate(79%) and a reduction in seminal root length inhibition. Moreover, transgenic plants under salt stress exhibited less physiological damage. SsNHX1-overexpressing transgenic maize accumulated more Na~+ and K~+ than wild-type(WT) plants particularly in the leaves, resulting in a higher ratio of K~+/Na~+ in the leaves under salt stress. This result revealed that the improved salt tolerance of SsNHX1-overexpressing transgenic maize plants was likely attributed to SsNHX1-mediated localization of Na~+ to vacuoles and subsequent maintenance of the cytosolic ionic balance. In addition, SsNHX1 overexpression also improved the drought tolerance of the transgenic maize plants, as rehydrated transgenic plants were restored to normal growth while WT plants did not grow normally after dehydration treatment. Therefore, based on our engineering approach, SsNHX1 represents a promising candidate gene for improving the salt and drought tolerance of maize and other crops.

关键词： Na^+/H^+ antiporter salt stress K^+/Na^+ drought stress gene transformation

Human Cytomegalovirus Infection Inhibits the Differentiation of Human Hippocampus Neural Precursor Cells into Astrocytes

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Virologica Sinica 2009年第6期24卷 552-558页

作者： Ling LI bin WANG Peng LI Zhi-qiang BAI Hai-tao WANG Hai-yan liu Dong-meng QIAN Xiu-xia SONG Shou-yi ding Zhi-yong yan Guang-yu JIANG Department of Histology and Embryology Qingdao University Medical College Qingdao 266071 China Department of Microbiology Qingdao University Medical College and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao 266071 China

HCMV is a major cause of congenital brain disease in humans, and its neuropathogenesis is not yet fully understood. The objective of the present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural precursor cell (NPCs) differentiation in vitro. Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. The identification and purity of the NPCs were confirmed by using immunofluorescence to detect the expression of the NPCs marker-Nestin. To drive NPCs differentiation, bFGF and EGF were withdrawn from the medium and replaced with FBS (10%). HCMV AD169 (MOI=5) was added into the differentiation medium at the onset of the differentiation. After 7 days of differentiation, in order to confirm whether NPCs are permissive for HCMV infection, immunofluorescence was used to stain for the presence of immediate early (IE) and late (pp65) HCMV proteins in the infected cells. The effects of HCMV infection on NPCs’ differentiation was observed by detecting the ratio of nestin and GFAP positive cells with confocal microscopy and immunofluorescence. The data showed that 95%±8% of the cells (passage 4-8) cultured were Nestin positive which suggested that majority of the cells were NPCs. On day 7 postinfection, most of the infected cells were IE and PP65 positive. The percentage of Nestin-positive cells were 93%±10% and 50%±19% (t=6.03, pdings indicate that NPCs are HCMV permissive cells and HCMV (AD 169) infection suppresses the differentiation of Hippocampus-genetic human NPCs into astrocytes. These effects may provide part of the explanation for the abnormalities in brain development associated with congenital HCMV infection.

关键词： Human cytomegalovirus (HCMV) Neural stem cells Cell differentiation Hippocampus Nestin

不对称入口速度剖面对颈动脉分叉模型不定常流场和壁面切应力的影响

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医用生物力学 2007年第3期22卷 242-245页

作者：夏琰丁祖荣刘宾上海交通大学船舶海洋与建筑工程学院上海200240

目的研究不对称入口速度剖面对动脉粥样硬化易发部位壁面切应力的影响。方法建立颈动脉TF-AHCB数值模型,设置不对称速度剖面入口条件,用CFD作数值模拟。计算不同时相、不同入口条件下的颈动脉窦流场和壁面切应力分布,分析与动脉粥样硬... 详细信息

目的研究不对称入口速度剖面对动脉粥样硬化易发部位壁面切应力的影响。方法建立颈动脉TF-AHCB数值模型,设置不对称速度剖面入口条件,用CFD作数值模拟。计算不同时相、不同入口条件下的颈动脉窦流场和壁面切应力分布,分析与动脉粥样硬化有关的流场特征和切应力特征。结果入口速度剖面偏斜可以增加颈动脉窦壁面低切应力区面积,提高切应力梯度。结论不对称入口速度剖面可能是诱发动脉粥样硬化发生的一个危险因子。

关键词：血流动力学入口条件壁面切应力数值模拟颈动脉窦壁

Proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus,as demonstrated by the surface enhanced laser desorption/ionization(SELDI)protein chip system

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Journal of Microbiology and Immunology 2007年第1期5卷 46-51页

作者： ZHI JUN liu bin WANG YING TIAN ZHI QIANG BAI SHOU YI ding Xu xia SONG ZHI YONG yan DONG MENG QIAN Department of Microbiology and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao P. R. China Department of Otolaryngology Affiliated Hospital of Qingdao University Medical College Qingdao P. R. China

The proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus （HCMV） was investigated at the protein level by using the surface enhanced laser desorption/ionization （SELDI） protein chip system in order to develop a method of study for the pathogenesis of HCMV infection. In this study, the cultured U251 cells were infected with HCMV in good condition and the supernatants of lysates and the extracellular fluids of the cultivated infected cells were quantitatively defined for the expressed proteins. The proteomics of the differential protein expression in cells before and after infection was analyzed by WCX2 arrays on the protein chip reader. It was demonstrated that the eytopathic effects of infected cells appeared on the 5th day after infection, however, the differential protein expression was evident at 6 h after infection as revealed by RT-PCR and mass spectrometry. The protein peaks captured from different batches of samples, from the same sample detected with different arrays or for the different times were all equivalent. With the molecular weight range from 2000 Da to 3000 Da, chip captured 82 peaks from the intracellular fluids and 11 protein peak from the cellular fluid in which compared with the control group, the protein peaks with molecular weight of 13 536.3 Da, 10 046.1 Da and 17 106.2 Da were close to those of β-amyloid protein, caspase-1 precursor and LPS-induced TNF-α factor respectively, which showed brief up-regulation 4 h after infection, and continued to raise 48 h later. These results infer that these proteins may be related to the apoptosis induced by HCMV infection, thus suggesting that the apoptosis induced by HCMV infection may play a role in the pathogenesis of HCMV infection.

关键词： SELDI protein chip U251 cells HCMV Protein expression

大口径方形离轴非球面的非球面度计算与应用研究

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光学技术 2007年第4期33卷 595-598页

作者：鄢定尧刘民才刘义彬刘夏来李瑞洁杨李茗成都精密光学工程研究中心成都610041

采用不同的数值计算方法求解了大口径(340mm×340mm)方形离轴非球面的最接近球面曲率半径和非球面度,得出了各种不同的磨削量及其对应的分布结构。对不同的最接近球面及非球面度所适用的加工工艺和检测方法进行了分析和评价。这对离轴... 详细信息

采用不同的数值计算方法求解了大口径(340mm×340mm)方形离轴非球面的最接近球面曲率半径和非球面度,得出了各种不同的磨削量及其对应的分布结构。对不同的最接近球面及非球面度所适用的加工工艺和检测方法进行了分析和评价。这对离轴非球面的加工和检测具有一定的指导意义。

关键词：离轴非球面非球面度加工检测

Status of aquatic organisms resources and their environments in yangtze River system(2017-2021)

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Aquaculture and Fisheries 2024年第5期9卷 833-850页

作者： Haile yang Li Shen Yongfeng He Huiwu Tian Lei Gao Jinming Wu Zhigang Mei Nian Wei Lin Wang Tingbing Zhu Feifei Hu Jinling Gong Hongchun Du Xinbin Duan Huatang Deng Daoqun Wang Fengyue Zhu Yunfeng Li Fan Wu Huijun Ru yan Zhang Junyi Li Junlin yang Yuntao Zhou Dongdong Fang Yinping Wang Danqing Lin yanping yang Peijie Li Silei liu Jian yang Ping Zhuang Sikai Wang Tao Zhang Gang yang Wenbo yang Lilai Yuan Kun Cao shuo Xu Huiyuan liu Zhiqiang Liang Chongrui Wang Hong Li Xiping Yuan Xin yang Yilong Fu yanping Zhang Haixin Zhang Zhiying Tao Sheng Wang xiaoping Gao binsong Jin Kemao Li Guojie Wang Shenglong Jian Yingqin Li Chenjiang Xue Chunyun Lei Shaowei Xue Yi Sun bin Zhu Ke Shao Xingkun Hu Meihua Xiong Jun Du bin He Tao yan Yingying Huang Yuanchao Zou Biwen Xie Yongming Wang bin Li Fei liu Yaoyao Zhang Fei Fan Zhijian Wang Jing Huang Haoran Gu Hailong Ge yan Dan yan Li Shuqiao Wang Chuang Zhang Lu Zhou Xue Wang Sheng Zeng yan xiang Xugang He Jianhui Qin Chengxing xia Jie Hou Yifu Shi Lifang Gao Zhiqiang Zhu Hongbao Shen Yun Du Xuejun Duan Jiawu Xiong Deguo yang Shaoping liu Zhaohui Ni Hui Zhang Kai liu Feng Zhao Yingren Li Jianwei Wang Qiwei Wei yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhan430223China Institute of Hydrobiology Chinese Academy of SciencesWuhan430072China Fishery Resource and Environment Research Center Chinese Academy of Fishery SciencesBeijing100141China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai200090China Institute of Fisheries Engineering Chinese Academy of Fishery SciencesBeijing100141China Hunan Fisheries Science Institute Changsha410153China Jiangxi Fisheries Research Institute Nanchang330039China Jiujiang Academy of Fishery Sciences Jiujiang332000China School of Life Sciences Nanchang UniversityNanchang330031China Qinghai Provincial Fishery Technology Extension Center Xining810012China Yunnan Academy of Fishery Sciences Kunming650111China Institute of Hydroecology Ministry of Water Resources and Chinese Academy of SciencesWuhan430079China Fisheries Institute Sichuan Academy of Agricultural SciencesChengdu611731China School of Life Sciences Neijiang Normal UniversityNeijiang641100China School of Life Sciences Southwest UniversityChongqing400715China Chongqing Fishery Sciences Research Institute Chongqing401120China Guizhou Fisheries Research Institute Guiyang550025China College of Fisheries Huazhong Agricultural UniversityWuhan430072China Hubei Fisheries Science Research Institute Wuhan430070China Shaanxi Fisheries Research Institute Xi’an710086China Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan430077China Nanjing Institute of Geography and Limnology Chinese Academy of SciencesNanjing210008China Central South Inventory and Planning Institute of State Forestry and Grassland Administration Changsha410014China

The yangtze River is the mother river of *** promote the aquatic ecosystem protection of the great river,the Project of yangtze Fisheries Resources and Environment Investigation(2017-2021)supported by the Ministry of Agriculture and Rural Affairs,*** carried out by 24 institutes and universities that located in the yangtze River basin surveys the status of(1)fish species composition and spatial distribution,(2)current fish abundance,(3)endangered fishes,(4)yangtze finless porpoise,(5)aquatic eco-environments,(6)water-level fluctuation areas,(7)capture fisheries and recreational fisheries of the yangtze River mainstream and 10 of its main tributaries,including Yalong River,Heng River,Min River(including Dadu River),Chishui River,Tuo River,Jialing River,Wu River,Han River,Dongting Lake and Poyang *** results showed that there were 443 fish species(belonging to 163 genus,37 families,and 18 orders)before 2017,but only 323 fish species(including 15 new recorded exotic species)were recorded in the project of *** them,Cyprinus carpio,Carassius auratus,Hypophthalmichthys molitrix,Pelteobagrus fulvidraco,Coilia brachygnathus,Silurus asotus,Saurogobio dabryi,Ctenopharyngodon idellus,Pelteobagrus nitidus,Hemiculter leucisculus,Siniperca chuatsi,Coreius heterodon,Culter alburnus,Parabramis pekinensis,and Aristichthys nobilis were the dominant species across the whole yangtze River *** is estimated that there were 886 million individuals weighing 124.8 million kg,merely equivalent to 27.3%of the resources in 1950s,30.9%of the resources in 1960s,or 58.7%of the resources in *** the new list of protected fishes that recorded in the yangtze River system,only 15 of 29 were collected in this project of *** gladius has been affirmed to be Extinct by International Union for Conservation of Nature(IUCN).The wild individuals of Tenualosa reevesii and Luciobrama macrocephalus have disappeared for many years and maybe have been extinct

关键词： Fishes yangtze finless porpoise Resources Aquatic ecosystem Fisheries Background investigation yangtze river system

免疫渗滤与胶体金快速检测在筛查甲型流感中诊断效率分析

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南方医科大学学报 2010年第10期30卷 2267-2269页

作者：贾宁闫中强刘刚沈定霞索继江邢玉斌高岩刘运喜解放军总医院感染管理与疾病控制科北京100853 解放军总医院微生物科北京100853 解放军总医院感染疾病科北京100853

目的评价胶体金和免疫渗滤法在筛查临床甲型流感病毒感染中的诊断效率。方法收集2009.6～2009.10我院发热门诊流感样病例咽拭子标本297例,分别用免疫渗滤法和胶体金法进行快速检测,同时采用实时荧光定量PCR和测序进行验证,以评价两种方... 详细信息

目的评价胶体金和免疫渗滤法在筛查临床甲型流感病毒感染中的诊断效率。方法收集2009.6～2009.10我院发热门诊流感样病例咽拭子标本297例,分别用免疫渗滤法和胶体金法进行快速检测,同时采用实时荧光定量PCR和测序进行验证,以评价两种方法的诊断效率。结果在实时荧光定量PCR法及测序证实为甲型流感166份,其中免疫渗滤法灵敏度为54.82%(91/166),与PCR法符合率为66.67%,Kappa值为0.35;胶体金法灵敏度为4.22%(7/166),符合率为45.79%,Kappa值为0.02;在131份甲型流感病毒阴性的标本中,免疫渗滤法特异性为81.68%(107/131),胶体金法特异性为98.47%(129/131);在实时荧光定量PCR检测为甲型H1N1阳性的99份标本中,免疫渗滤法检出率为67.3%,胶体金检出率为5.1%;在两种方法结果不符合108份标本中,免疫渗滤法阳性而胶体金阴性的标本107份,实时荧光定量PCR法并序列测定显示其中84份为甲型流感病毒阳性,23份为阴性;免疫渗滤法阴性而胶体金阳性的1份标本,甲型流感病毒实时荧光PCR检测为阴性。免疫渗滤法在甲型流感病毒的检出率和与PCR方法的符合率方面明显高于胶体金法(P<0.05)。结论免疫渗滤法在甲型流感病毒筛查中优于胶体金法,显示出良好的临床应用前景。

关键词：甲型流感病毒胶体金免疫渗滤实时荧光PCR