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Establishment of a tetracycline-off and heat shock-on gene expression system in tobacco

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Journal of Integrative Agriculture 2017年第5期16卷 1112-1119页

作者： ZHOU You LI Jin-hua PAN yu ZHENG yu PAN Yang-lu ding yu-mei SU Cheng-gang ZHANG Xing-guo Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715P.R.China

The tetracycline （Tet）-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and repeatedly replenishing tetracycline to turn off the constitutively active system is a tedious process. To solve these problems, a Tet-off and heat shock （HS）-on gene expression regulation system was constructed in this study. This system is composed of a chimeric transactivator gene TetR-HSF that is derived from a Tet repressor （TetR） and a HS transcription factor （HSF） controlled by a HS promoter HSP70m, and a Tet operator containing hybrid promoter, Om35S, that drives expression of the β-glucuronidase （GUS） gene. The resultant system yields a GUS expression pattern similar to that of the HSP70m promoter under inducing temperatures and at 35 and 40℃ drives GUS expression to a similar level as the Cauliflower mosaic virus （CaMV） 35S promoter. Further examination revealed that the TetR-HSF and GUS genes were induced by HS, reaching peak expression after 1 and 6 h treatment, respectively, and the HS induction of the expression system could be inhibited by Tet. This system will provide a useful tool for transgenic studies of plants in the laboratory and in the field, including transgene function analysis, agronomic trait improvement, biopharmaceutical protein production and others.

关键词： gene expression system heat shock-on tetracycline-off TetR-HSF transactivator tobacco

中部地区居民上颌前牙矢状向牙根位置与周围牙槽骨的CBCT研究

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临床口腔医学杂志 2024年第8期40卷 464-468页

作者：李澄饶佳慧师改平于然郑雪晴丁玉梅华中科技大学同济医学院附属协和医院口腔科湖北武汉430022

目的:通过CBCT影像资料分析中部地区居民健康上颌前牙矢状向牙根位置,测量唇腭侧牙槽骨壁的厚度、牙槽骨尺寸、根尖区骨高度,为上颌前牙区即刻种植的术前计划和预后判断提供依据。方法:选取70名中部地区居民的健康上颌前牙的CBCT影像资... 详细信息

目的:通过CBCT影像资料分析中部地区居民健康上颌前牙矢状向牙根位置,测量唇腭侧牙槽骨壁的厚度、牙槽骨尺寸、根尖区骨高度,为上颌前牙区即刻种植的术前计划和预后判断提供依据。方法:选取70名中部地区居民的健康上颌前牙的CBCT影像资料,分析上颌前牙牙根在矢状向的位置分类;测量唇腭侧骨壁的厚度、牙槽骨尺寸、根尖区骨高度,并分析其与矢状向牙根位置的相关性。结果:92.4%为Ⅰ类,仅3.3%为Ⅱ类,4.3%为IV类;距牙槽嵴顶2 mm处唇侧骨壁厚度Ⅰ类最小(P=0.014),根中1/3处唇侧骨壁厚度Ⅱ类最大(P=0.001),根尖处唇侧骨壁厚度为Ⅳ类<Ⅰ类<Ⅱ类(P<0.001);聘侧骨壁厚度为Ⅳ类<Ⅱ类<Ⅰ类(P<0.001)。牙槽骨宽度为Ⅰ类最大(P<0.01);牙槽骨长度为Ⅰ类<Ⅲ类<Ⅳ类,无统计学差异;根尖区骨高度依次为Ⅰ类<Ⅳ类<Ⅱ类(P=0.005)。结论:评估矢状向牙根位置及周围牙槽骨的情况包括唇聘侧骨壁厚度、牙槽骨尺寸、根尖区骨高度等对上颌前牙区的即刻种植具有重要指导意义。

关键词：矢状向牙根位置上颌前牙牙槽骨厚度根尖区骨高度即刻种植锥形束CT

Equivalent linear model for seismic damage evaluation of single-degree-of-freedom systems representing reinforced concrete structures considering cyclic degradation behavior

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Earthquake Engineering and Engineering Vibration 2024年第3期23卷 637-648页

作者： Lulu Yan ding-Hao yu Research Center for Geotechnical and Structural Engineering Technology of Liaoning Province Dalian UniversityDalian116622China State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China

In this study,a novel equivalent damping ratio model that is suitable for reinforced concrete(RC)structures considering cyclic degradation behavior is developed,and a new equivalent linearization analysis method for implementing the proposed equivalent damping ratio model for use in seismic damage evaluation is *** this end,Ibarra’s peak-oriented model,which incorporates an energy-based degradation rule,is selected for representing hysteretic behavior of RC structure,and the optimized equivalent damping for predicting the maximum displacement response is presented by using the empirical method,in which the effect of cyclic degradation is ***,the relationship between the hysteretic energy dissipation of the inelastic system and the elastic strain energy of the equivalent linear system is established so that the proposed equivalent linear system can be directly integrated with the Park-Ang seismic model to implement seismic damage *** to the simplicity of the equivalent linearization method,the proposed method provides an efficient and reliable way of obtaining comprehensive insight into the seismic performance of RC *** verification demonstrates the validity of the proposed method.

关键词： degrading system equivalent linear system equivalent damping seismic damage RC structures

Curcumin modulates oxidative stress to inhibit pyroptosis and improve the inflammatory microenvironment to treat endometriosis

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Genes & Diseases 2024年第3期11卷 102-105页

作者： Jie ding Shanshan mei Kaili Wang Wen Cheng Shuai Sun Zhexin Ni Xiaoqiu Wang Chaoqin yu Department of Gynecology of Traditional Chinese Medicine Changhai Hospital Affiliated to Naval Medical UniversityShanghai 200433China Obstetrics and Gynecology Department The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhouZhejiang 310006China Department of Pharmaceutical Sciences Beijing Institute of Radiation MedicineBeijing 100850China Obstetrics and Gynecology Hospital of Fudan University Shanghai 200433China

Endometriosis(EM)is a common disease that affects approximately 10%-15%of women of childbearing *** pathogenesis of EM is unclear,but studies have shown a strong association between EM and inflammation,as well as oxidative stress.^(1) Pyroptosis is also called inflammatory cell *** pyroptosis occurs,it activates a strong inflammatory *** is associated with oxidative stress,and Ros act as intermediate triggers to activate pyroptosi.

关键词： inflammatory death oxidative

Type I interferon pathway in pediatric systemic lupus erythematosus

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World Journal of Pediatrics 2024年第7期20卷 653-668页

作者： yu Zhou Hong‑mei Song Department of Pediatrics Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College HospitalBeijingChina

Background The role of type I interferon(IFN-I)signaling in systemic lupus erythematosus(SLE)has been well ***,unanswered questions remain regarding the applicability of these findings to pediatric-onset *** aim of this review is to provide an overview of the novel discoveries on IFN-I signaling in pediatric-onset *** sources A literature search was conducted in the PubMed database using the following keywords:“pediatric systemic lupus erythematosus”and“type I interferon”.Results IFN-I signaling is increased in pediatric SLE,largely due to the presence of plasmacytoid dendritic cells and pathways such as cyclic GMP-AMP synthase–stimulator of interferon genes–TANK-binding kinase 1 and Toll-like receptor(TLR)4/*** extracellular traps and oxidative DNA damage further stimulate IFN-I *** variants in IFN-I-related genes,such as IFN-regulatory factor 5 and tyrosine kinase 2,are linked to SLE susceptibility in pediatric *** addition,type I interferonopathies,characterized by sustained IFN-I activation,can mimic SLE symptoms and are thus important to *** on interferonopathies also contribute to exploring the pathogenesis of *** IFN-I activation is crucial for SLE diagnosis and *** IFNstimulated gene expression and serum IFN-α2 levels are common *** cytometry markers such as CD169 and galectin-9 are promising ***-IFN therapies,such as sifalimumab and anifrolumab,show promise in adult patients with SLE,but their efficacy in pediatric patients requires further *** kinase inhibitors are another treatment option for severe pediatric SLE *** This review presents an overview of the IFN-I pathway in pediatric *** the intricate relationship between IFN-I and pediatric SLE may help to identify potential diagnostic markers and targeted therapies,paving the way for improved patient care and outcomes.

关键词： Interferonopathies Pediatric systemic lupus erythematosus Type I interferon

Carbon felt electrode coated with WS2 enables a high-performance polysulfide/ferricyanide flow battery

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Rare Metals 2024年第10期43卷 5039-5047页

作者： Liang-yu Li Su Yan Ying-Jia Huang Fang-Fang Zhong Jin-Chao Cao mei ding Chuan-Kun Jia College of Materials Science and Engineering Changsha University of Science and Technology Institute of Energy Storage Technology College of Energy and Power EngineeringChangsha University of Science and Technology Binzhou Institute of Technology Weiqiao-UCAS Science and Technology Park

Polysulfide/ferricyanide flow batteries （S/Fe RFBs）,with the advantages of abundant earth reservation low cost,high safety,and environmental friendliness,have attracted significant interest and demonstrated noteworthy potential for practical ***,the battery performance,including the energy efficiency （EE）,voltage efficiency （VE）,and power density of the S/Fe RFBs remains low owing to the slow redox kinetics of polysulfide *** address these concerns,WS2was selected as the booster and deposited on a commercial carbon felt electrode （WS2–CF） to stimulate the redox reactions of polysulfide *** better hydrophilicity and smaller charge-transfer resistance,WS2–CF exhibits enhanced electrochemical activity toward polysulfide redox ***,the battery performance of S/Fe RFB with WS2–CF as the anode has been improved,with EE of 84%,VE of 84%,and a peak power density of 175.7 mW·cm-2,which are all higher than the cell only with the bare carbon felt （CF） as electrodes (76%,77% and 155.8 mW·cm-2,respectively).Furthermore,the cycling life of the S/Fe RFB with WS2–CF has been prolonged to 2200 cycles with a capacity retention of 96% a 40 mA·cm-2because of the good stability of WS2–CF as the ***,under the same conditions,the S/Fe RFB without WS2–CF terminated after 1500 cycles with a fast capacity *** successful utilization of WS2as a booster on an electrode provides an efficient strategy for obtaining advanced S/Fe RFBs for practical applications.

关键词： Carbon felt Energy storage Polysulfide/ferricyanide flow battery Tungsten disulfide

Chord length sampling correction analysis for dispersion fuel in Monte Carlo simulation

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Nuclear Science and Techniques 2024年第4期35卷 57-64页

作者： Zhao-yu Liang ding She yu-Tong Wen Lei Shi Institute of Nuclear and New Energy Technology(INET) Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of EducationTsinghua UniversityBeijing 100084China

Dispersion fuels,knowned for their excellent safety performance,are widely used in advanced reactors,such as hightemperature gas-cooled *** with deterministic methods,the Monte Carlo method has more advantages in the geometric modeling of stochastic *** explicit modeling method has high computational accuracy and high computational *** chord length sampling(CLS)method can improve computational efficiency by sampling the chord length during neutron transport using the matrix chord length?s probability density *** study shows that the excluded-volume effect in realistic stochastic media can introduce certain deviations into the CLS.A chord length correction approach is proposed to obtain the chord length correction factor by developing the Particle code based on equivalent transmission *** numerical analysis against reference solutions from explicit modeling in the RMC code,it was demonstrated that CLS with the proposed correction method provides good accuracy for addressing the excludedvolume effect in realistic infinite stochastic media.

关键词： Stochastic media Monte Carlo Chord length sampling Excluded-volume effect Chord length correction

Microearthquake reveals the lithospheric structure at midocean ridges and oceanic transform faults

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Journal of Oceanology and Limnology 2024年第3期42卷 697-700页

作者： Zhiteng yu Jiabiao LI Weiwei ding Key Laboratory of Submarine Geosciences&Second Institute of Oceanography Ministry of Natural ResourcesHangzhou 310012China

Mid-ocean ridge and oceanic transforms are among the most prominent features on the seafloor surface and are crucial for understanding seafloor spreading and plate tectonic dynamics,but the deep structure of the oceanic lithosphere remains poorly *** large number of microearthquakes occurring along ridges and transforms provide valuable information for gaining an indepth view of the underlying detailed seismic structures,contributing to understanding geodynamic processes within the oceanic *** studies have indicated that the maximum depth of microseismicity is controlled by the 600-℃***,this perspective is being challenged due to increasing observations of deep earthquakes that far exceed this suggested isotherm along mid-ocean ridges and oceanic transform *** mechanisms have been proposed to explain these deep events,and we suggest that local geodynamic processes(e.g.,magma supply,mylonite shear zone,longlived faults,hydrothermal vents,etc.)likely play a more important role than previously thought.

关键词： microearthquake mid-ocean ridge oceanic transform fault oceanic lithosphere thermal structure earthquake location

A generalization of Banach's lemma and its applications to perturbations of bounded linear operators

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Applied Mathematics(A Journal of Chinese Universities) 2024年第2期39卷 363-369页

作者： WANG Zi ding Jiu WANG yu-wen School of Mathematics Sciences Harbin Normal UniversityHarbin 150025China School of Mathematics and Natural Sciences The University of Southern MississippiHattiesburg MS 39406-5043USA Academic Committee Harbin Institute of PetroleumHarbin 150027China

Let X be a Banach space and let P:X→X be a bounded linear *** an algebraic inequality on the spectrum of P,we give a new sufficient condition that guarantees the existence of(I-P)^(-1) as a bounded linear operator on X,and a bound on its spectral radius is also *** generalizes the classic Banach *** apply the result to the perturbation analysis of general bounded linear operators on X with commutative perturbations.

关键词： Banach lemma spectral radius generalized inverse perturbation analysis