检索结果-南通市图书馆

维普期刊数据库博看期刊

基于肾脏类器官研究镁离子减轻顺铂诱导的急性肾损伤的作用和机制

在线全文

学校读者我要写书评

暂无评论

复旦学报（医学版） 2024年第4期51卷 455-464,483页

作者：吴欢嵇姞鲁敏宁一纯孙肇星丁小强俞小芳复旦大学附属中山医院肾内科上海200032

目的利用肾脏类器官和HK-2细胞研究镁离子(Mg2+)对顺铂诱导的急性肾损伤(cisplatin-induced acute kidney injury,Cis-AKI)的作用,并探究可能的机制。方法首先利用人源性诱导多能干细胞(induced pluripotent stem cells,iPSCs)构建肾脏类器官,在此基础上构建Cis-AKI模型,利用HE染色观察肾脏类器官结构,通过免疫荧光染色观察标志物定位及cleaved caspase-3表达,通过qRT-PCR检测肾小管和肾小球标志物及炎症因子mRNA水平。随后将肾脏类器官及HK-2细胞随机分为对照组、顺铂组(Cis组)和Mg2+预处理组(Cis+Mg2+组)。通过CCK-8和ATP含量评估肾小管上皮细胞的活力;利用TUNEL染色检测肾小管上皮细胞凋亡情况;通过Western blot检测细胞凋亡通路的关键蛋白Bcl-2、Bax、cleaved caspase-3及有机阳离子转运体2(organic cation transporter 2,OCT2)表达;通过免疫荧光检测OCT2的定位与表达。结果肾脏类器官培养第10天的肾小管结构清晰,肾脏标志物大量表达;10μmol/L顺铂导致肾脏类器官结构破坏,cleaved caspase-3表达量和炎症因子mRNA水平显著升高,ATP含量显著下降。与Cis组相比,Cis+Mg2+组肾脏类器官ATP含量升高,TUNEL阳性细胞数减少,细胞凋亡相关蛋白表达显著下降,OCT2表达显著下降;而Cis+Mg2+组HK-2细胞活力、TUNEL阳性细胞数及凋亡相关蛋白均无明显改善,且几乎不表达OCT2。结论肾脏类器官是研究Cis-AKI发病与治疗的理想体外模型;Mg2+预处理可显著减轻顺铂所致肾脏类器官的损伤,其机制可能与OCT2的下调有关。

关键词：顺铂急性肾损伤(AKI) 镁离子(Mg2+) 有机阳离子转运体2(OCT2) 肾脏类器官

Atmospheric transmission algorithm for pulsed X-rays from high-altitude nuclear detonations based on scattering correction

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2024年第3期35卷 40-52页

作者： ding-Han Zhu Xiong Zhang xiao-qiang Li Peng Li Yan-Bin Wang Shuang Zhang State Key Laboratory of NBC Protection for Civilian Beijing 102205China

In high-altitude nuclear detonations,the proportion of pulsed X-ray energy can exceed 70%,making it a specific monitoring signal for such *** pulsed X-rays can be captured using a satellite-borne X-ray detector following atmospheric *** quantitatively analyze the effects of different satellite detection altitudes,burst heights,and transmission angles on the physical processes of X-ray transport and energy fluence,we developed an atmospheric transmission algorithm for pulsed X-rays from high-altitude nuclear detonations based on scattering *** proposed method is an improvement over the traditional analytical method that only computes direct-transmission *** traditional analytical method exhibits a maximum relative error of 67.79% compared with the Monte Carlo *** improved method reduces this error to within 10% under the same conditions,even reaching 1% in certain ***,its computation time is 48,000 times faster than that of the Monte Carlo *** results have important theoretical significance and engineering application value for designing satellite-borne nuclear detonation pulsed X-ray detectors,inverting nuclear detonation source terms,and assessing ionospheric effects.

关键词： High-altitude nuclear detonation Atmospheric transmission Pulsed X-rays Scattering correction Analytical method Monte Carlo method

Research on feed-pulse collaborative control method in micro-electrical discharge machining

在线全文

学校读者我要写书评

暂无评论

Advances in Manufacturing 2024年第2期12卷 270-287页

作者： qiang Gao Ya-Ou Zhang Xue-Cheng Xi Yuan-ding Wang xiao-Fei Chen Wan-Sheng Zhao School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai200240People’s Republic of China State Key Laboratory of Mechanical System and Vibration Shanghai200240People’s Republic of China Shanghai Institute of Space Propulsion Shanghai201112People’s Republic of China

Reducing the short-circuit rate and increasing the effective discharge rate are important targets for improving the servo control effect of micro-electrical discharge machining(micro-EDM),as these two indicators are closely related to the machining efficiency and *** this study,a feed-pulse collaborative control(FPCC)method is proposed for micro-EDM based on two dimensions(space and time).In the spatial dimension,a feed control strategy with a discharge holding process is ***,in the time dimension,a forward-looking pulse control strategy is adopted,in which the pulse interval is adjusted based on a sequence analysis of feed commands and discharge *** experiments are carried out to determine the key parameters used in this method,including the discharge holding threshold and pulse interval adjustment value(T_(off_(adj))).The feed smoothness and discharge sufficiency analyses of the experimental results show that compared to the traditional double threshold average voltage method,the FPCC method reduces the number of long-distance retreats by 64%and improves the effective discharge time by 40%.

关键词： Micro-electrical discharge machining(micro-EDM) Electrode feed control Discharge pulse control Machining surface quality

A brand new green coating technology for realizing the regulation of spherical propellant energy release process

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2024年第6期36卷 78-94页

作者： Wenhao Fan Yajun ding Zhongliang xiao School of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjing210094China Key Laboratory of Special Energy Materials Nanjing University of Science and TechnologyMinistry of EducationNanjing 210094China

To achieve the controllable release of energy of nitrocellulose-based propellants,this paper combines the cellulose-based nanocomposites aqueous coating(Surelease®-NC)with fluidized bed coating equipment to successfully prepare the coated spherical propellant for the first *** effects of fluidized bed coating temperature,air velocity,flow speed and atomization pressure on the adhesion rate,coating integrity and coating uniformity of the coated spherical propellant were investigated,and the preparation of coated spherical propellant with homogeneous size and structural integrity was achieved for the first *** microscopic morphology,chemical structure,water vapor adsorption behavior,combustion performance,and ageing resistance property of the coated spherical propellant were systematically investigated by,Fourier transforms infrared spectroscopy(FTIR),Micro confocal raman spectrometer,field scanning electron microscopy(SEM),dynamic vapor adsorption techniques,and closed bomb test,confirming the surface core-shell structure and the tightly bonded interfacial structure of coated spherical ***,the coated spherical propellant has good hygroscopic,excellent progressive burning and long storage stability.

关键词： Nanocomposites aqueous coating Fluidized bed coating equipment Coated spherical propellant Controllable release of energy Long storage stability

Inter-annual variations of dissolved oxygen and hypoxia off the northern Changjiang River(Yangtze River) Estuary in summer from 1997 to 2014

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2024年第6期43卷 119-130页

作者： Anqi Liu Feng Zhou xiao Ma qiang Zhao Guanghong Liao Yuntao Zhou Di Tian xiaobo Ni Ruibin ding College of Oceanography Hohai UniversityNanjing 210098China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China Observation and Research Station of Changjiang River Delta Marine Ecosystems Ministry of Natural ResourcesZhoushan 316022China Ningbo Marine Center Ministry of Natural ResourcesNingbo 315000China School of Oceanography Shanghai Jiao Tong UniversityShanghai 200240China Institute of Polar and Ocean Technology Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China

Hypoxia off the Changjiang River Estuary has been the subject of much attention,yet systematic observations have been lacking,resulting in a lack of knowledge regarding its long-term change and *** revisiting the repeated surveys of dissolved oxygen(DO) and other relevant hydrographic parameters along the section from the Changjiang River Estuary to the Jeju-do in the summer from 1997 to 2014,rather different trends were revealed for the dual low-DO *** nearshore low-DO core,located close to the river mouth and relatively stable,shows that hypoxia has become more severe with the lowest DO descen ding at a rate of -0.07 mg/(L·a) and the thickness of low-DO zone rising at a rate of 0.43 m/*** offshore core,centered around 40-m isobath but moving back and forth between 123.5°-125°E,shows large fluctuations in the minimum DO concentration,with the thickness of low-DO zone falling at a rate of -1.55 m/*** probable factors affecting the minimum DO concentration in the two regions also *** the nearshore region,the decreasing minimum DO is driven by the increase in both stratification and primary productivity,with the enhanced extension of the Changjiang River Diluted Water(CDW) strengthening *** the offshore region,the fluctuating trend of the minimum DO concentration indicates that both DO loss and DO supplement are *** DO loss is primarily attributed to bottom apparent oxygen utilization caused by the organic matter decay and is also relevant to the advection of low-DO water from the nearshore *** DO supplement is primarily due to weakened *** analysis also shows that the minimum DO concentration in the nearshore region was extremely low in 1998,2003,2007 and 2010,related to El Ni?o signal in these summers.

关键词： dissolved oxygen low-DO Changjiang River Estuary interannual variations dual-core

Optical system for extremely large spectroscopic survey telescope

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2024年第7期67卷 158-174页

作者： ding-qiang Su Hua Bai Xiangyan Yuan Xiangqun Cui Key Laboratory of the Ministry of Education School of Astronomy and Space ScienceNanjing UniversityNanjing 210023China Nanjing Institute of Astronomical Optics and Technology(NIAOT) Chinese Academy of SciencesNanjing 210042China University of Chinese Academy of Sciences Nanjing 211135China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 101408China

This article presents research work on a spectroscopic survey *** idea is as follows:for such a telescope,a pure reflecting optical system is designed,which should have an aperture and a field of view(FOV)both as large as possible and excellent image quality,and then a strip lensm(lens-prism)atmospheric dispersion corrector(S-ADC)is added,only for correcting the atmospheric *** the fund limitation and the simplicity of scaling up,some 12-m telescopes are designed as ***,Korsch,and Meinel put forward the four-mirror Nasmyth systems I and II,which are used in this *** of 1.5°,2°,and 2.5°are *** all systems,the image qualities are *** the S-ADC relaxes the optical glass size restriction,this 12-m telescope with a FOV of 2.5°can be magnified in proportion to a 16-m *** etendue(from Frenchétendue)and focal surface will now be the largest in the *** such a telescope,a pure reflecting optical system can also be obtained.A subsequent coudésystem is designed with excellent image quality.

关键词： telescopes spectroscopic survey

Current understanding of ELF4 deficiency:a novel inborn error of immunity

在线全文

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2024年第5期20卷 444-450页

作者： Hong-qiang Du xiao-Dong Zhao Department of Rheumatology&Immunology National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChongqing Key Laboratory of Child Rare Diseases in Infection and ImmunityChildren’s Hospital of Chongqing Medical UniversityZhongshan Er Road 136Yuzhong DistrictChongqingChina

Background ELF4 deficiency has been recently recognized as a novel disorder within the spectrum of inborn errors of immunity(IEIs),specifically categorized as a“disease of immune dysregulation.”Cases of this condition,reported by our team and others,are very limited *** such,our current knowledge of this new disease remains *** review aims to provide a brief overview of the clinical manifestations,pathogenesis,and treatment strategies for this novel *** sources A comprehensive review was conducted after an extensive literature search in the PubMed/Medline database and websites concerning transcriptional factor ELF4 and reports concerning patients with ELF4 *** search strategy was“ELF4 OR ETS-related transcription factor Elf-4 OR EL4-like factor 4 OR myeloid Elf-1-like factor”as of the time of manuscript *** The current signature manifestations of ELF4 deficiency disorder are recurrent and prolonged oral ulcer,abdominal pain,and diarrhea in pediatric *** some cases,immunodeficiency and autoimmunity can also be *** Sanger sequencing or whole exome sequencing can be used to detect variation in ELF4 *** blotting for ELF4 expression of the patient’s cells can confirm the pathogenic effect of the *** fully confirm the pathogenicity of the variant,further functional test is strongly *** and biologics are the mainstream management of ELF4 deficiency *** Pediatric males presenting with recurring ulcerations in digestive tract epithelium with or without recurrent fever should be suspected of *** atypical presentations are prominent,variations in ELF4 gene should be carefully evaluated functionally due to the complex nature of ELF4 *** of treating DEX includes use of glucocorticoid and biologics and more precise treatment needs more patients to identify and further mechanistic study.

关键词： ELF4 transcription factor Immune dysregulation Inborn errors of immunity Mechanism Recurrent infections

Numerical analysis of geosynthetic-reinforced embankment performance under moving loads

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rock Mechanics and Geotechnical Engineering 2024年第2期16卷 682-696页

作者： Xuanming ding Jinqiao Zhao qiang Ou Jianfei Liu College of Civil Engineering Chongqing UniversityChongqing400045China Key Laboratory of New Technology for Construction of Cities in Mountain Area Chongqing UniversityChongqing400045China China United Engineering Corporation Limited Hangzhou310052China

The performance of geosynthetic-reinforced embankments under traffic moving loads is always a hotspot in the geotechnical engineering field.A three-dimensional(3D)model of a geosynthetic-reinforced embankment without drainage consolidation was established using the finite element software *** this model,the traffic loads were simulated by two moving loads of rectangular pattern,and their amplitude,range,and moving speed were realized by a Fortran *** embankment fill was simulated by an equivalent linear viscoelastic model,which can reflect its *** geogrid was simulated by the truss element,and the geocell was simulated by the membrane *** elements were utilized to weaken the boundary effect caused by the model geometry at the *** of the established numerical model was conducted by comparing the predicted deformations in the cross-section of the geosynthetic-reinforced embankment with those from the existing *** this basis,the dynamic stress and strain distribution in the pavement structure layer of the geosynthetic-reinforced embankment under a moving load was also ***,a parametric study was conducted to examine the influences of the different types of reinforcement,overload,and the moving load velocity on the geosynthetic-reinforced embankment.

关键词： Geosynthetic-reinforced layer Numerical model Moving load Embankment Deformation Stress

Analytical model for predicting time-dependent lateral deformation of geosynthetics-reinforced soil walls with modular block facing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rock Mechanics and Geotechnical Engineering 2024年第2期16卷 711-725页

作者： Luqiang ding Chengzhi xiao Feilong Cui School of Civil and Transportation Engineering Hebei University of TechnologyTianjin300401China

To date,few models are available in the literature to consider the creep behavior of geosynthetics when predicting the lateral deformation(d)of geosynthetics-reinforced soil(GRS)retaining *** this study,a general hyperbolic creep model was first introduced to describe the long-term deformation of geosynthetics,which is a function of elapsed time and two empirical parameters a and *** conventional creep tests with three different tensile loads(Pr)were conducted on two uniaxial geogrids to determine their creep behavior,as well as the a-Pr and b-Pr *** test results show that increasing Pr accelerates the development of creep deformation for both ***,a and b respectively show exponential and negatively linear relationships with Pr,which were confirmed by abundant experimental data available in other *** on the above creep model and relationships,an accurate and reliable analytical model was then proposed for predicting the time-dependent d of GRS walls with modular block facing,which was further validated using a relevant numerical investigation from the previous *** evaluation and comparison of the proposed model with six available prediction models were *** a parametric study was carried out to evaluate the effects of wall height,vertical spacing of geogrids,unit weight and internal friction angle of backfills,and factor of safety against pullout on d at the end of construction and 5 years *** findings show that the creep effect not only promotes d but also raises the elevation of the maximum d along the wall ***,the limitations and application prospects of the proposed model were discussed and analyzed.

关键词： Geosynthetics Creep behavior Geosynthetics-reinforced soil(GRS)walls Lateral deformation Analytical model