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DNA microarray unravels rapid changes in transcriptome of MK-801 treated rat brain

World Journal of Biological Chemistry 2015年第4期6卷 389-408页

作者： Yuka Kobayashi Sofya P Kulikova Junko Shibato Randeep Rakwal Hiroyuki Satoh didier pinault Yoshinori Masuo Department of Biomolecular Science Faculty of ScienceToho University Health Technology Research Center National Institute of Advanced Industrial Science and Technology INSERM U1114 Neuro-psychologie Cognitive et Physiopathologie de la SchizophrénieFMTS (Fédération de Médecine Translationnelle de Strasbourg)Faculté de MédecineUniversité de Strasbourg Department of Anatomy School of MedicineShowa University Laboratory of Exercise Biochemistry and Neuroendrocrinology Institute of Health and Sport SciencesUniversity of Tsukuba Global Research Center for Innovative Life Science Peptide Drug InnovationSchool of Pharmacy and Pharmaceutical SciencesHoshi University Organization for Educational Initiatives University of Tsukuba Faculty of Health and Sport Sciences and Tsukuba International Academy for Sport Studies University of Tsukuba Laboratory of Neuroscience Department of BiologyFaculty of ScienceToho University

AIM:To investigate the impact of MK-801 on gene expression patterns genome wide in rat brain regions. METHODS:Rats were treated with an intraperitoneal injection of MK-801 [0.08(low-dose) and 0.16(highdose) mg/kg] or NaC l(vehicle control). In a first series of experiment,the frontoparietal electrocorticogram was recorded 15 min before and 60 min after injection. In a second series of experiments,the whole brain of each animal was rapidly removed at 40 min post-injection,and different regions were separated:amygdala,cerebral cortex,hippocampus,hypothalamus,midbrain and ventral striatum on ice followed by DNA microarray(4 × 44 K whole rat genome chip) ***:Spectral analysis revealed that a single systemic injection of MK-801 significantly and selectively augmented the power of baseline gamma frequency(30-80 Hz) oscillations in the frontoparietal electroencephalogram. DNA microarray analysis showed the largest number(up- and down- regulations) of gene expressions in the cerebral cortex(378),midbrain(376),hippocampus(375),ventral striatum(353),amygdala(301),and hypothalamus(201) under low-dose(0.08 mg/kg) of MK-801. Under high-dose(0.16 mg/kg),ventral striatum(811) showed the largest number of gene expression changes. Gene expression changes were functionally categorized to reveal expression of genes and function varies with each brain ***:Acute MK-801 treatment increases synchrony of baseline gamma oscillations,and causes very early changes in gene expressions in six individual rat brain regions,a first report.

关键词： Dizocilpine Dye-swap Gene expression Microarray MK

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