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Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process

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Acta Pharmaceutica Sinica B 2022年第4期12卷 2089-2102页

作者： Mélanie Robert Bastien Laperrousaz diana piedrahita Emilie-Fleur Gautier Travis Nemkov Florian Dupuy Elie Nader Virginie Salnot Patrick Mayeux Angelo D’Alessandro Catherine Lavazec Philippe Joly Alexander Scheer Philippe Connes Agnès Cibiel Erytech Pharma Lyon 69008France Laboratoire interuniversitaire de Biologie de la Motricité(LIBM)EA7424 Team«Vascular Biology and Red Blood Cell»Universite´Claude Bernard Lyon 1Universite´de LyonLyon 69008France Labex GR-Ex Paris 75015France 3P5 proteom’IC facility Universitéde ParisInstitut CochinINSERMCNRSParis 75014France University of Colorado Anschutz Medical CampusAuroraCO 80045USA Omix Technologies Inc.AuroraCO 80045USA Inserm U1016 CNRS 8104Universitéde ParisInstitut CochinParis 75014France Laboratoire de Biochimie et de Biologie Moléculaire UF de biochimie des pathologies e´rythrocytairesCentre de Biologie et de Pathologie EstHospices Civils de LyonBron 69500France

Red blood cells(RBCs)can act as carriers for therapeutic agents and can substantially improve the safety,pharmacokinetics,and pharmacodynamics of many *** RBCs integrity and lifespan is important for the efficacy of RBCs as drug *** investigated the impact of drug encapsulation by hypotonic dialysis on RBCs physiology and *** parameters were compared between processed RBCs loaded with l-asparaginase(“eryaspase”),processed RBCs without drug and non-processed *** RBCs were less hydrated and displayed a reduction of intracellular *** observed a change in the metabolomic but not in the proteomic profile of processed *** process caused moderate morphological changes and was accompanied by an increase of RBCs-derived Extracellular Vesicles *** a decrease in deformability,processed RBCs were not mechanically retained in a spleen-mimicking device and had increased surface-to-volume ratio and osmotic *** RBCs half-life was not significantly affected in a mouse model and our previous phase 1 clinical study showed that encapsulation of asparaginase in RBCs prolonged its in vivo half-life compared to free *** study demonstrated that encapsulation by hypotonic dialysis may affect certain characteristics of RBCs but does not significantly affect the in vivo longevity of RBCs or their drug carrier function.

关键词： Red blood cells Drug carrier Hypotonic dialysis L-Asparaginase Omics Rheology Morphology Senescence markers

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Kinetic Study of the Oxidative Degradation of Choiba Oil(Dipteryx oleifera Benth.)with Addition of Rosemary Extract(Rosmarinus officinalis L.)

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Food and Nutrition Sciences 2015年第5期6卷 466-479页

作者： Ana María piedrahita Jeanine Penaloza Alvaro Cogollo Benjamín Alberto Rojano Agrarian Sciences Faculty National University of ColombiaMedellínColombia Tecnas S.A. MedellínColombia Botanical Garden of Medellín“Joaquín Antonio Uribe” MedellinColombia Chemistry Ecole National University of ColombiaMedellínColombia

Choibá (Dipteryx oleifera Benth.) is a promising source of edible oil with high nutritional quality and a significant content of oleic acid (52% - 54%). To promote Choibá as source of edible oil is necessary to ensure its stability along the time of production, distribution and storage. Loss of nutritional and organoleptic quality in lipids is mainly due to lipid peroxidation reactions. The aim of this research was to evaluate the oxidative stability of Choibá oil at 100°C ± 1°C with aeration (1150 mL air/min) supplemented with rosemary extract (Rosmarinus officinalis L.), at 1000 mg/L (RE1000) and 1500 mg/L (RE1500), and with BHT (200 mg/L) and from this results to evaluate the degradation kinetics and shelf-life of Choibá oil at 35°C, 45°C and 55°C without addition of antioxidants (Control) and with addition of best concentration of rosemary extract obtained from previous study. Progress in oil oxidation was measured through the extent of oxidation products: peroxide value (PV) and thiobarbituric acid reactive substances (TBARS). Results revealed that the addition of rosemary extract at 1500 mg/L significantly reduced de formation of hydroperoxides (PV), more than BHT. Through correlations between concentrations of antioxidant (including control without antioxidant) with peroxide values, the kinetics of degradation and shelf-life of Choibá oil with predictive models are evaluated in real time and accelerated (35°C, 45°C and 55°C) using the Arrhenius equation. In addition, the oxidation reactions of this oil follow a first order kinetic model for PV and zero order kinetic model for TBARS. The rate of formation of PV was dependent on the storage temperature, according to the Arrhenius equation with the activation energy of 4611.5071 J/mol for Control and 7409.5771 J/mol for RE1500 treatment. The result of TBARS didn’t adjust to Arrhenius model, thus measurement of malondialdehyde (MDA) wasn’t a useful parameter for shelf-life determination of Choibá oil.

关键词： Dipteryx oleifera Oxidative Stability Shelf-Life Rosmarinus officinalis Lipid Peroxidation Arrhenius

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北美牙鲆对两种深度的水道式养殖池和圆形养殖池中溶解氧垂直分布的影响

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渔业现代化 2008年第1期35卷 62-62页

作者： L.Reig R.H.piedrahita D.E.Conklin

水产养殖池的设计和运转,应该将与养殖鱼紧邻的水流静滞区降低至最小。在养殖中上层鱼类的水池中,水的流动和鱼的游动相结合,在与养殖鱼紧邻的水流静滞区域,足以维持溶氧和新陈代谢浓度与主水体相一致。如果是底层栖息性鱼类,如北美牙鲆... 详细信息

水产养殖池的设计和运转,应该将与养殖鱼紧邻的水流静滞区降低至最小。在养殖中上层鱼类的水池中,水的流动和鱼的游动相结合,在与养殖鱼紧邻的水流静滞区域,足以维持溶氧和新陈代谢浓度与主水体相一致。如果是底层栖息性鱼类,如北美牙鲆,它们一般静止地呆在养殖池底,常常在它们伏蜇的区域会出现数个水质恶化的水层。本研究的目的是评估北美牙鲆可能对10 cm和20 cm两种水深的水道式养殖池(239 cm长,28 cm宽)和圆形养殖池(直径92 cm)中垂直分布的氧浓度的影响。在有鱼和没有鱼的水池区域,沿垂直方向的每一厘米单位对氧进行测量,以评估北美牙鲆对溶解氧垂直分布的影响。结果显示,在水道养殖池和圆形养殖池的近池底区域,氧浓度较低。水深20 cm的水道式池该现象最明显,但在其它三个池中也发现此现象。同时还对位于鱼嘴前方或直接取自鱼嘴中的水样本进行了测量,记录到了和鱼紧邻的低氧浓度区,其氧浓度为所测量的水池排放水中氧浓度的50%。氧浓度的下降量,水道式养殖池比圆形养殖池大,20 cm水深的养殖池比10 cm水深的养殖池大。鱼在低氧浓度区静呆时间过长,会经常表现出强力呼吸。鱼周围的氧浓度始终比排放出的水的氧浓度低。因此,测量排放...

关键词：水产养殖池垂直分布溶解氧水道牙鲆北美圆形中上层鱼类

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