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The triphibious warfare against viruses

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Science China(Life Sciences) 2017年第12期60卷 1295-1298页

作者： William J.liu di liu Key Laboratory of Medical Virology and Viral diseases Ministry of Health of People's Republic of China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention CAS Key Laboratory for Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Computational Virology Group CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of Virology Chinese Academy of Sciences

Despite the long journey undertaken by mankind to confront the diseases caused by viruses,people were unaware of the existence of viruses until the closing years of the 19th century,when the Russian biologist Dmitri Ivanovsky demonstrated that tobacco plants could be infected by a toxin smaller than bacteria,followed by which the Dutch microbiologist Martinus Beijerinck termed the toxin as a contagium vivum fluidum （soluble living germ） and re-introduced the word ＂virus＂（derived from the Latin word ＂virus＂ meaning poison; the modern meaning is an ＂agent that causes infectious disease＂ that was first recorded in 1728）.

关键词： The triphibious warfare viruses

黑果枸杞DBB转录因子家族鉴定与生物信息学分析

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兰州大学学报（自然科学版） 2024年第3期60卷 415-426页

作者：刘筠李效雄杜雨马瑞胡晓桐柳迪贾西贝马彦军甘肃农业大学林学院兰州730070 兰州资源环境职业技术大学兰州730030

对黑果枸杞Double B-box(DBB)基因家族测序并进行生物信息学分析,以深入研究LrDBB基因的功能.以NaCl溶液处理黑果枸杞转录组数据库为基础,对黑果枸杞DBB基因家族成员进行鉴定和分析.黑果枸杞转录组数据中共筛选出32个DBB家族成员,分为5... 详细信息

对黑果枸杞Double B-box(DBB)基因家族测序并进行生物信息学分析,以深入研究LrDBB基因的功能.以NaCl溶液处理黑果枸杞转录组数据库为基础,对黑果枸杞DBB基因家族成员进行鉴定和分析.黑果枸杞转录组数据中共筛选出32个DBB家族成员,分为5个亚家族,蛋白质亚细胞定位预测中其家族成员主要定位于细胞核.黑果枸杞DBB家族蛋白质分子量为5 156.83~53 308.44D;等电点4.58~8.96;总原子数692~7 324;氨基酸个数46~476 aa;脂溶指数51.06~92.88;大多数蛋白质成员为不稳定蛋白质和亲水蛋白质.LrDBB家族的蛋白质N端均具有B-box保守结构域,无规则卷曲和α-螺旋占比较大.表达模式中发现,NaCl胁迫株的根和叶中均有LrDBB基因家族成员出现高表达量.在NaCl胁迫下黑果枸杞DBB基因于不同器官中的表达量存在明显差异,推断此家族基因可能在盐胁迫中起调控作用.

关键词：黑果枸杞 NaCl胁迫 DBB转录因子生物信息学分析

数智赋能驱动下品质工程建设与管理研究——以深中通道为例

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现代工程科技 2024年第14期3卷 109-112页

作者：宋神友金文良刘迪深中通道管理中心广东中山528400

随着国家工程建设的深入发展,数智赋能逐步成为品质工程建设与管理的基础及核心。首先分析工程品质与品质工程的内涵,深入剖析数智化背景下深中通道品质工程建设与管理的意义;其次,从工程复杂性、系统复杂性、管理复杂性论述深中通道品... 详细信息

随着国家工程建设的深入发展,数智赋能逐步成为品质工程建设与管理的基础及核心。首先分析工程品质与品质工程的内涵,深入剖析数智化背景下深中通道品质工程建设与管理的意义;其次,从工程复杂性、系统复杂性、管理复杂性论述深中通道品质工程建设新理念的重大变革;通过阐述深中通道数智建造品质保障机制,探索数智赋能对深中通道品质工程建设与管理的基本作用与原理;最后,通过阐明数智赋能如何渗透深中通道品质工程建设与管理实践,形成数智赋能驱动下深中通道品质工程建设与管理新范式。

关键词：品质工程强国交通深中通道数智建造

烟草番茄斑萎病毒RT-LAMP检测体系的建立及优化

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中国农业科技导报 2024年第8期26卷 140-150页

作者：张俊蕾盖晓彤赵正婷刘弟王金凤姜宁刘雅婷云南农业大学植物保护学院昆明650201 云南省烟草农业科学研究院昆明650021 云南农业大学农学与生物技术学院昆明650201 云南农业大学烟草学院昆明650201

为快速检测烟草番茄斑萎病毒(tomato spotted wilt virus,TSWV),基于TSWV核外壳蛋白(nucleocapsid protein,NP)保守序列设计5组引物进行筛选,采用单一变量法对反应温度、时间及反应体系中dNTPs、Mg^(2+)、甜菜碱的含量和内外引物比等参... 详细信息

为快速检测烟草番茄斑萎病毒(tomato spotted wilt virus,TSWV),基于TSWV核外壳蛋白(nucleocapsid protein,NP)保守序列设计5组引物进行筛选,采用单一变量法对反应温度、时间及反应体系中dNTPs、Mg^(2+)、甜菜碱的含量和内外引物比等参数进行优化,建立烟草TSWV反转录环介导等温扩增(loop-mediated isothermal amplification,RT-LAMP)检测体系,并采用RT-PCR(revers transcription-polymerase chain reaction)检测方法进行平行比对试验,验证优化后RT-LAMP的特异性、灵敏性及实用性。结果表明,烟草TSWV RT-LAMP检测体系最佳引物组为TS-N-4,在25μL的反应体系中各组分最佳加入量为缓冲液2.5μL、100 mmol·L^(-1) MgSO40.5μL、10 mmol·L^(-1) dNTPs 0.5μL、10 mmol·L^(-1) FIP/BIP 1.5μL、10 mmol·L^(-1) F3/B30.5μL、10 mmol·L^(-1) LF/LB 1.5μL、5 mmol·L^(-1) Betaine 6μL、Bst 2.0 WarmStar DNA Polymerase(8000 U·mL-1)0.5μL、M-MLV酶(10000 U·mL^(-1))0.125μL、RNA(≥64.7 fg)1μL,DEPC H2O补至25μL;最佳反应温度和时间分别为58℃、60 min。优化后的RT-LAMP灵敏度是RT-PCR的1000倍,且田间样品检测结果与RT-PCR相符。建立的RT-LAMP方法特异性强、灵敏度高、操作简单,对于烟草TSWV的检测及监控有重要意义。

关键词：番茄斑萎病毒 NP基因反转录-环介导等温扩增快速检测

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Nao-Fu-Cong ameliorates diabetic cognitive dysfunction by inhibition of JNK/CHOP/Bcl2-mediated apoptosis in vivo and in vitro

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Chinese Journal of Natural Medicines 2020年第9期18卷 704-713页

作者： JING Guang-Chan liu di liu Yu-Qin ZHANG Meng Ren Deparment of Traditional Chinese Medicine Peking Union Medical Collge HospitalPeking Union Medical Collge and Chinese Academy of Medical SciencesBeijing 100730China Deparment 0f cell resource center Institute of Basic Medical SciencePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China

Chinese herbal compound Nao-Fu-Cong(NFC) has been mainly used to treat cognitive disorders in Traditional Chinese Medicine(TCM). The present study aimed to investigate whether its neuroprotective effects might be related to the inhibition of JNK/CHOP/Bcl2-mediated apoptosis pathway or not. We randomly assigned STZ(60 mg·k^(-1))-induced diabetic rats into control group, diabetic model group and NFC groups(low-dose, medium-dose and high-dose). The primary culture of hippocampal neurons were transferred into different culture media on the third day. The cells were then divided into control group, high-glucose group, NFC(low-dose, medium-dose and high-dose) groups, CHOP si-RNA intervention group, JNK pathway inhibitor SP600125 group and oxidative stress inhibitor N-acetylcysteine(NAC) group. NFC significantly improved the cognitive function of diabetic rats, and had neuroprotective effect on hippocampal neurons cultured in high glucose. Further research results showed that NFC could reduce the apoptosis of hippocampal neurons in rats with diabetic cognitive dysfunction. NFC had inhibitory effects on CHOP/JNK apoptosis pathway induced by high glucose, and also decreased the levels of ROS and increased the mitochondrial membrane potential. These suggested that the neuroprotective effect of NFC might be related to the inhibition of CHOP and JNK apoptotic signaling pathways, and the cross pathway between oxidative stress and mitochondrial damage pathway.

关键词： Nao-Fu-Cong Apoptosis High glucose Hippocampal neurons

Continuous synthesis of ultrasmall core-shell upconversion nanoparticles via a flow chemistry method

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Nano Research 2022年第2期15卷 1199-1204页

作者： di liu Junyu Yan Kai Wang Yundong Wang Guangsheng Luo The State Key Lab of Chemical Engineering Department of Chemical EngineeringTsinghua UniversityBeijing 100084China

A continuous synthesis method for the less than 10 nm core-shell upconversion nanoparticles was developed via coiled tube embedded flask reactors and a flow solvothermal co-participation reaction up to 300℃.Fast nucleation of hexagonal nanocrystals in less than 9 min residence time was achieved owing to the excellent heating ability of the reactors,and a two-step reaction strategy was created for the synthesis of β-NaYF4:Gd,Yb,Er/Ho/Tm@NaYF_(4) particles without intermediate purification.

关键词： sodium rare earth tetrafluoride upconversion nanoparticle core-shell structure flow chemistry synthesis

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基于物联网的医用耗材管理平台的构建与应用

中国数字医学 2024年第6期19卷 73-79页

作者：孙琳郭敬鹏刘荻杨超首都医科大学附属北京同仁医院信息中心北京100730

目的:优化医用耗材院内外流转使用流程,实现医用耗材精细化与智能化管理。方法:通过信息技术和耗材管理终端的应用,以医用高值耗材管理为切入点,采用以医院为主导,引入第三方运营服务的SPD管理模式,构建医院全流程的医用耗材管理平台,... 详细信息

目的:优化医用耗材院内外流转使用流程,实现医用耗材精细化与智能化管理。方法:通过信息技术和耗材管理终端的应用,以医用高值耗材管理为切入点,采用以医院为主导,引入第三方运营服务的SPD管理模式,构建医院全流程的医用耗材管理平台,与院内物流管理系统和医院信息管理系统(HIS)无缝集成,实现高值耗材RFID条码全流程实时追溯,并佐以数据验证,实现信息技术与医疗场景有效融合。结果:平台实现了耗材采供协同、智能存取、动态更新、预警提示、智慧分析与辅助决策等功能,优化了耗材管理流程与架构,以高值耗材管理为例,耗材日均管理用时缩短82.5%,信息维护用时缩短84.1%,临床工作人员申请用时缩短79.5%,精细化管理的耗材品规及数量提高了57.0%。结论:以医院为主导,引入第三方服务的SPD管理模式提高了医院医用耗材的流转和使用效率,达到控耗降耗、提高医用耗材质控管理的预期。在提升医院服务质量和医用耗材使用安全的同时,实现了医用耗材的精细化全生命周期管理。

关键词： SPD 精细化管理医院物资管理智能化管理 RFID

Thermo-structural analysis of regenerative cooling thrust chamber cylinder segment based on experimental data

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Chinese Journal of Aeronautics 2020年第1期33卷 102-115页

作者： di liu Bing SUN Taiping WANG Jiawen SONG Jianwei ZHANG School of Astronautics Beihang UniversityBeijing 100083China The 9th Designing China Aerospace Science and Industry CorporationWuhan 430040China

To evaluate the structural failure risk of the regenerative cooling thrust chamber cylinder segment,a Finite Element Method(FEM)based on experimental data was *** methodology was validated and utilized to reveal the thermal response and the nonlinear deformation behavior of the cylinder segment phase by *** conclusions of the research are as follows:The 2 D heat flux distribution caused by the injector determines the uneven temperature distribution on the gas-side wall and leads to the temperature disparity between various cooling channels;The reason for the accumulation of residual strain is that the tensile strain generated in the post-cooling phase is greater than the compressive strain produced in the hot run phase;Through the single-cycle simulation,two potential failure locations with conspicuous deformations were found,but it is difficult to determine which point is more ***,the multi-cycle thermo-structural analysis gives the evolution of the stress-strain curve and gradually discloses that the low-temperature corner of a particular channel is the most likely location to fail,rather than the maximum residual strain point of the gas-side *** damage analysis for dangerous point indicates that the quasistatic damage accounts for the majority of the total damage and is the main factor limiting the service life.

关键词： Finite element analysis Rocket engine Regenerative cooling Structural analysis Thrust chamber Thermal analysis

Effect of the lenticles on moisture migration in capillary zone of tailings dam

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International Journal of Minerals,Metallurgy and Materials 2020年第8期27卷 1036-1045页

作者： di liu Min-jie Lian Cai-wu Lu Wen Zhang College of Materials Science and Engineering Xi’an University of Architecture and TechnologyXi’an 710055China Institute of Mining System Engineering Xi’an University of Architecture and TechnologyXi’an 710055China Sinosteel Mining Co. Ltd.Beijing 100080China School of Resource Engineering Xi’an University of Architecture and TechnologyXi’an 710055China

Small-particle interlayers(lenticles)show some characteristic hydraulic properties and can affect the movement of unsaturated *** this study,we developed a novel online capillary-water-absorption monitoring device and conducted three groups of comparison tests to simulate lenticle positions and thicknesses with respect to the capillary *** results show that the characteristic wetting front exhibits a fast rise in the early stage,a slow rise in the middle stage,and stability in the later *** motion of the capillary water in the lenticle is mainly transversal,with the upward curve being"flat,"and the longer is"flat,"the longer is the time needed for the water to *** interlayer can form a capillary stagnation zone with moisture content close to *** high interlayer may form a discontinuous corrugated capillary ***,when the wetting front reaches the"coarse-grain(lower)-fine-grain(upper)"interface,the"anti-capillary barrier effect"results in more moisture in the upper ***,when the wetting front of the capillary water reaches the"fine-grain(upper)-coarse-grain(lower)"interface,the"capillary barrier effect"causes the moisture content of the upper tailings to decreases sharply because of the horizontal movement of the water in the fine *** is clear that the presence of lenticles can retard the rise of capillary water by storing water.

关键词： tailings lenticle moisture migration capillary retarding effect microcosmic mechanism