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Valorisation of fruit waste for enhanced exopolysaccharide production by Xanthomonas campestries using statistical optimisation of medium and process

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Food Bioscience 2022年第2期46卷 1014-1019页

作者： Avni Vaishnav Kinjal Upadhyay Manoj Koradiya devayani tipre Shailesh Dave Department of Microbiology School of ScienceGujarat UniversityAhmedabad380009GujaratIndia Department of Biochemistry and Biotechnology St.Xavier’s CollegeAhmedabad380009GujaratIndia Department of Microbiology L.J.Institutes of Applied SciencesS.G.RoadAhmedabad382210GujaratIndia Xavier Research Foundation Loyola Centre for R&DSt.Xavier’s College CampusAhmedabad380009GujaratIndia

The aim of this research work was to enhance the fermentative production of exopolysaccharide(EPS)with mixed fruit waste as a key *** campestries was isolated from spoiled orange and its EPS production efficiency was determined using EPS *** to optimisation,the production was 3.4 g/L,upon optimizing the medium the production improved to 9.2 g/*** medium formulation was studied by sequential addition of medium components and replacement of sugar with mixed fruit *** formulated medium,the production enhanced to 11.1 g/*** further optimisation,the response surface methodology was used,which resulted in the enhancement of production to 14.5 g/*** scale-up study was undertaken in a 10 L shake flask and *** 10 L flask EPS,production was 14.45 g/L in 100 h,whereas in 10 L fermenter it was 21.10 g/L in 60h *** optimisation studies gave nearly 500%(6.2fold)enhanced EPS production and dwindle in fermentation *** of sucrose with fruit waste extract made the process environment-friendly;omission of L-cystine and use of 50%reduced amount of sodium acetate in the medium lowered the production cost of *** developed process resulted in the utilization of fruit waste and was also useful in solving the fruit waste disposal problem.

关键词： Exopolysaccharide Mixed fruits waste Formulated medium Scale up Response surface methodology

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