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Relationship between antimicrobial resistance and aminoglycoside-modifying enzyme gene expressions in Acinetobacter baumannii

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中华医学杂志(英文版) 2005年第2期000卷 55-59页

作者： SHI Wei feng, JIANG Jian ping and MI Zu huang department of Clinical Laboratory, third affiliated hospital, suzhou university, changzhou 213003, china Wuxi Cloning Technique Research Institute, Wuxi 214026, china department of Clinical Laboratory Third Affiliated Hospital Suzhou University Changzhou 213003 China Wuxi Cloning Technique Research Institute Wuxi 214026 China

Background Acinetobacter baumannii is one of the main gramnegative bacilli in clinical practice Nosocomial infections caused by multidrug resistance Acinetobacter baumannii is very difficult to treat This study was designed to investigate the antimicrobial resistance characteristics and four resistant gene expressions of aminoglycosidemodifying enzymes including Nacetyltransferases and Ophosphotransferases in Acinetobacter baumannii Methods Bacterial identification and antimicrobial susceptibility test were performed by PhoenixTM system in 247 strains of Acinetobacter baumannii Minimal inhibitory concentrations (MICs) of seven aminoglycosides including gentamicin, amikacin, kanamycin, tobramycin, netilmicin, neomycin and streptomycin in 15 strains of multidrug resistant Acinetobacter baumannii were detected by agar dilution Four aminoglycosidemodifying enzyme genes were amplified by polymerase chain　reaction (PCR) and verified by DNA sequencerResults The resistance rates of 247 strains of Acinetobacter baumannii against cefotaxime, levofloxacin, piperacillin, aztreonam, tetracycline, ciprofloxacin and chloramphenicol were more than 50% Imipenem and meropenem showed high antibacterial activities with resistance rates of 32% and 41% MIC50 and MIC90 of gentamicin, amikacin, streptomycin and kanamycin in 15 strains of multidrug resistant Acinetobacter baumanii were all more than 1024 mg/L, and the resistance rates were 100%, 100%, 100% and 933%, respectively But their resistance rates to tobramycin, netilmicin and neomycin were 867%, 933% and 467%, respectively Three modifying enzyme genes, including aacC1, aacC2 and aacA4 genes, were found in 15 strains, but aphA6 had not been detected Their positive rates were 933%, 200% and 200%, respectively These three genes existed simultaneously in No19 strain Nucleotide sequences of aacC1, aacC2 and aacA4 genes shared 100%, 979% and 997% identities with GenBank genes (AY307

关键词： Acinetobacter baumannii · drug resistance · aminoglycoside-modifying enzyme · DNA sequence

Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients

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World Journal of Gastroenterology 2010年第48期16卷 6155-6162页

作者： Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, department of Tumor Biological Treatment, third affiliated hospital of Soochow university, changzhou 213003, Jiangsu Province, china Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow university, suzhou 215123, Jiangsu Province, china Yue-Ping Shen, Wen-Xiang Wei, department of Medicine, Soochow university, suzhou 215123, Jiangsu Province, china Bin-Feng Lu, department of Immunology, university of Pitts- burgh School of Medicine, Pittsburgh, PA 15261, United States Key Laboratory of Stem Cell of Jiangsu Province Institute of Biotechnology Key Laboratory of Clinical Immunology of Jiangsu Province Soochow University Suzhou 215123 Jiangsu Province China.

AIM: To analyze the correlation between cytokineinduced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients. METHODS: One hundred and fifty-six gastric cancer patients after operation at the third affiliated hospital of Soochow university were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model. RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ 2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P < 0.05). The 2- and 5-year survival rates of gastric cancer patients were significantly higher after CIK cells adoptive immunotherapy than after chemotherapy (73.5% vs 52.6%, 40.4% vs 23.9%, P < 0.05). A significant difference was observed in the survival curve for patients who received CIK cells adoptive immunotherapy (0, 1-10, 11-25, and over 25 frequencies) (χ 2 = 14.534, P = 0.002). The frequencies of CIK cells adoptive immunotherapy were significantly related with the decreasing risk of death in gastric cancer patients after adjustment for sex and age of the patients, tumor stage and relapse (HR = 0.54, 95% CI: 0.36-0.80) when the first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of deat

关键词： Immunotherapy Cytokine-induced killer cells Gastric cancer Survival analysis Probability

可拉明与面(鼻)罩BiPAP通气在呼吸衰竭时的合理应用

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苏州大学学报（医学版） 2002年第4期22卷 430-432页

作者：周军苏州大学附属三院呼吸科常州213003

目的　探讨可拉明以及经面 (鼻 )罩BiPAP呼吸机通气在慢性阻塞性肺病 (COPD)合并呼吸衰竭时的合理应用。方法　COPD合并慢性呼吸衰竭急性加重期的病人 ,在使用可拉明或 (和 )经面 (鼻 )罩BiPAP呼吸机通气治疗前后测血气分析。结果　A组... 详细信息

目的　探讨可拉明以及经面 (鼻 )罩BiPAP呼吸机通气在慢性阻塞性肺病 (COPD)合并呼吸衰竭时的合理应用。方法　COPD合并慢性呼吸衰竭急性加重期的病人 ,在使用可拉明或 (和 )经面 (鼻 )罩BiPAP呼吸机通气治疗前后测血气分析。结果　A组 10例呼吸频率较慢 ,经可拉明静滴后血气分析及症状明显好转 (P 0 .0 5 ) ,经面 (鼻 )罩BiPAP呼吸机通气治疗后缓解 (P 0 .0 5 ) ,呼吸频率减慢 (P <0 .0 1) ,加用可拉明后血气分析及症状明显好转(P <0 .0 1)。结论　呼吸频率较慢的COPD合并呼吸衰竭病人使用呼吸兴奋剂疗效较好 ,呼吸频率较快的病人使用呼吸兴奋剂效果差 ,使用BiPAP呼吸机通气治疗后症状好转 ,少数病例经BiPAP面罩通气治疗后症状好转不满意。

关键词：合理应用慢性阻塞性肺病呼吸衰竭可拉明 BiPAP

RMP is oncogenic for the growth of hepatoma cells

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RMP is oncogenic for the growth of hepatoma cells

A proteomic analysis of allograft rejection in rats after liver transplantation

2010’全国肿瘤分子标志及应用学术研讨会暨第五届中国中青年肿瘤专家论坛

作者： Huicui Yang,Jingting Jiang,Xiaoning Lian,Yanyan Bai,Jingcheng Miao,Bairong Shen,Wenxiang Wei department of Cellular and Molecular Biology,School of Medicine,suzhou university,suzhou, 215123 china department of Tumor Biotherapy,the third affiliated hospital of suzhou university,changzhou, 213003 china.

Objectives:To explore the biological role of RPB5-mediating protein(RMP) in the growth and the biological property of hepatoma cell SMMC-7721. Methods:Hepatoma cells SMMC-7721 were cultured in *** overexpression and R...

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Science china(Life Sciences) 2007年第3期50卷 312-319页

作者： ZHANG ChunChao1,2, ZHU Feng2,3, WEI JianFeng2, ZHENG ShuSen2 & LI LanJuan1 1 department of Infectious Diseases, The First affiliated hospital, Zhejiang university School of Medicine, Hangzhou 310003, china 2 Institute of Surgery, Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First affiliated hospital, Zhejiang university School of Medicine, Hangzhou 310003, china 3 department of Hepatobiliary Surgery, The third affiliated hospital, suzhou university, changzhou 213003, china department of Infectious Diseases The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China Institute of Surgery Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health The First Affiliated HospitalZhejiang University School of Medicine Hangzhou 310003China Institute of Surgery Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China department of Hepatobiliary Surgery The Third Affiliated Hospital Suzhou University Changzhou 213003 China Institute of Surgery Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China department of Infectious Diseases The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

In order to understand the allograft rejection in orthotopic liver transplantation (OLT), an allograft re- jection rat model was established and studied by proteomic approach. The protein expression profiles of liver tissues were acquired by fluorescence two-dimensional difference gel electrophoresis (2D DIGE) that incorporated a pooled internal standard and reverse fluorescent labeling method. The expression levels of 27 protein spots showed significant changes in acute rejection rats. Among these spots, 19 were identified with peptide mass fingerprinting using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) after tryptic in-gel digestion. The results of the present paper could be helpful for our better understanding of allograft rejection in organ transplantation.

关键词： A proteomic analysis of allograft rejection in rats after liver transplantation DIGE

血清TSGF、CYFRA21-1和NSE联合检测对肺癌的诊断价值

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苏州大学学报（医学版） 2005年第2期25卷 265-267页

作者：左志通凌春华刘皓郑世营苏州大学附属第一医院呼吸科江苏苏州215006 苏州大学附属第三医院呼吸科

目的　探讨联合检测血清中肿瘤标志物在肺癌诊断中的价值。方法　采用生化比色法、电化学发光法检测肺癌及肺部良性病变患者血清中肿瘤特异性生长因子(TSGF)、细胞角蛋白19片段(CYFRA2 1 1)、神经元特异性烯醇化酶(NSE)的含量。结果　... 详细信息

目的　探讨联合检测血清中肿瘤标志物在肺癌诊断中的价值。方法　采用生化比色法、电化学发光法检测肺癌及肺部良性病变患者血清中肿瘤特异性生长因子(TSGF)、细胞角蛋白19片段(CYFRA2 1 1)、神经元特异性烯醇化酶(NSE)的含量。结果　肺癌患者血清中3种肿瘤标志物明显高于肺部良性病变患者(P <0 .0 1或P<0 .0 5 ) ;TSGF的敏感性、特异性分别为84 .8%、86 .1% ;不同病理类型CYFRA2 1 1、NSE的敏感性亦不同。结论　血清中3种肿瘤标志物的联合检测,在肺癌的诊断、病理类型的推断方面有一定的临床意义;TSGF可能为良好的健康体检筛选指标。

关键词：肺癌肿瘤特异性生长因子细胞角蛋白19片段神经元特异性烯醇化酶

16层CT冠状动脉造影的技术及影响图像质量的因素

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实用放射学杂志 2007年第12期23卷 1692-1695页

作者：孙益芳邢伟陈文华吴小凤王士英许金梅何小兰王慧聂建新苏州大学第三附属医院影像科苏州大学第三附属医院影像科江苏常州213003

目的探讨16层CT冠状动脉造影的成像技术及影响图像质量的因素。方法52例受检者按心率≤70次/min和>70次/min分为2组,分别行16层螺旋CT心电门控条件下冠状动脉造影,预览R-R间期0%～100%的21幅图像(间隔5%),选择显示左、右冠状动脉最清晰... 详细信息

目的探讨16层CT冠状动脉造影的成像技术及影响图像质量的因素。方法52例受检者按心率≤70次/min和>70次/min分为2组,分别行16层螺旋CT心电门控条件下冠状动脉造影,预览R-R间期0%～100%的21幅图像(间隔5%),选择显示左、右冠状动脉最清晰的一幅,应用原始数据重建该时相的薄层图像,再进行MIP、MPR、CPR、VR重建。2组图像质量均分为能用于冠状动脉评价和不能用于冠状动脉评价2类。结果16层CT冠状动脉成像的最佳重建时相多位于55%～65%R-R间期。心率≤70次/min组,有87%(301/346支)的图像可用于诊断,心率>70次/min组,有78.8%(134/170支)的图像可用于诊断。结论影响冠状动脉评价的因素主要有冠状动脉运动、冠状动脉钙化、心率波动等。

关键词：心脏冠状动脉心血管造影术体层摄影术,X线计算机

^(131)碘治疗巨大甲状腺肿性Graves病250例疗效分析

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苏州大学学报（医学版） 2003年第1期23卷 63-66页

作者：王勤奋张承刚赵晓斌施龙宝苏州大学附属三院核医学科江苏常州213003 山西医科大学附属一院核医学科山西太原030001 山西医科大学附属三院甲状腺病治疗中心山西太原030008

目的　评价131碘治疗巨大甲状腺肿性Graves病的效果和个体化的剂量估算方法。方法　 2 5 0例巨大甲状腺肿性Graves病患者 ,甲状腺重量 113 .0± 3 9.2 (90～ 45 0 ) g。据患者个体因素 ,确定每克甲状腺组织131碘剂量的范围一般为 2 .775... 详细信息

目的　评价131碘治疗巨大甲状腺肿性Graves病的效果和个体化的剂量估算方法。方法　 2 5 0例巨大甲状腺肿性Graves病患者 ,甲状腺重量 113 .0± 3 9.2 (90～ 45 0 ) g。据患者个体因素 ,确定每克甲状腺组织131碘剂量的范围一般为 2 .775～ 5 .18MBq/g。随访时间 15 .9± 9.9(3～ 44 .7)个月。结果　一次131碘治疗后 ,15 4例(61.6% )甲状腺功能恢复正常 ,5 3例 (2 1.2 % )甲状腺功能亢进症 (甲亢 )仍持续 ,43例 (17.2 % )发生甲状腺功能减退症 (甲减 ) ;一次131碘治疗后 2 19例 (87.6% )肿大的甲状腺恢复正常。结论　131碘对巨大甲状腺肿性Graves病有效 ;

关键词：巨大甲状腺肿 Graves病碘放射性同位素

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大动物原位肝移植的术中静脉转流

苏州医学院学报 1999年第7期19卷 794-795页

作者：秦锡虎唐剑星邹岩孙冬林黄伯华苏州医学院附属第三医院肝胆外科

报道１５例犬及５例猪同种异体原位肝移植，其中７例犬及４例猪行背驮式肝移植，并于术中行门静脉—颈静脉体位转流，其余动物行其它不同转流方式或不转流。结果显示前者的血流动力学稳定性明显优于其它术式，有利于完成肝移植。

关键词：肝移植静脉转流原位移植实验研究