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脑电记录中不同参考电极位置的同步对比分析

现代电生理学杂志 2002年第4期9卷 157-158页

作者：王淑娟刘英高刘佩云张栩青大医学院附院脑电图室 266003

目的:探讨脑电记录中不同参考电极位置对图形分布特征的影响。方法:对500例病人进行了相同部位参考电极导联脑电图的同步对比分析。结果:耳垂参考电极有活化现象和伪迹干扰,但波幅高,中线两侧脑波失真较少。Cz 参考电极干扰少有利于颞... 详细信息

目的:探讨脑电记录中不同参考电极位置对图形分布特征的影响。方法:对500例病人进行了相同部位参考电极导联脑电图的同步对比分析。结果:耳垂参考电极有活化现象和伪迹干扰,但波幅高,中线两侧脑波失真较少。Cz 参考电极干扰少有利于颞部的异常电位的暴露,但不利于头顶部病变的发现。颧骨弓处盘状电极减少了活化和伪迹干扰。结论:颞部病变以 Cz 为参考点的导联阳性率最高,而中线两侧各部的病变则以耳垂为参考点的单极导联阳性率最高,因此脑电图描记时应变换各种参考电极位置进行比较。

关键词：脑电记录参考电极位置同步对比分析

Neural cell cycle dysregulation and central nervous system diseases

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Neural cell cycle dysregulation and central nervous system d...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Wei WANG,Bi-Tao BU,Min-Jie XIE,Min ZHANG,Zhi-Yuan YU,De-Ding TAO~2 1 department of neurology,Tongji hospital,Tongji medical college,Huazhong,university of Science and Technology, Wuhan,430030,china 2 Molecular Medicine Center,Tongji hospital,Tongji medical college,Huazhong university of Science and Technology, Wuhan,430030,china

＜正＞The cell cycle is a delicately manipulated process essential for the development,differentiation,proliferation and death of *** activation of cell cycle regulators is implicated in the pathophysiology of a wide range of central nervous system（CNS） diseases,including both acute damage and chronic neurodegenerative *** cycle activation induces the dividing astrocytes and microglia to activate and proliferate in association with glial scar formation and inflammatory factor production,which play crucial roles in the development of pathology in CNS *** the other hand,in terminally differentiated neurons,aberrant reentry into the cell cycle triggers neuronal death instead of proliferation,which may be a common pathway shared by some acquired and neurodegenerative disorders,even though multiple pathways of the cell cycle machinery are involved in distinct neuronal demise in specific pathological circumstances. In this paper,we first provide a concise description of the roles of cell cycle in neural *** then focus on how neural cell cycle dysregulation is related to CNS *** apoptosis is often detected in acute injury to the CNS such as stroke and trauma,which are usually related to the blockade of the cell cycle at the G1-S *** neurodegenerative diseases such as Alzheimer’s disease,Parkinson’s disease,amyotrophic lateral sclerosis and Niemann-Pick disease type C,however,some populations of neurons complete DNA synthesis but the cell cycle is arrested at the G2/M transition. This review summarizes advances in findings implicating cell cycle machinery in neuronal death in CNS diseases.

关键词： cell cycle glia neuron apoptosis ischemia neurodegeneration

Point Mutation in the Parkin Gene on Patients with Parkinson's Disease

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Journal of Huazhong university of Science and Technology(medical Sciences) 2003年第2期23卷 145-147页

作者：王涛梁直厚孙圣刚曹学兵彭海曹非刘红进童萼塘 department of neurology Xiehe HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430022

To investigate the distribution of possible novel mutations from parkin gene in variant subset of patients with Parkinson's disease (PD) in china and explore whether parkin gene plays an important role in the pathogenesis of PD, 70 patients were divided into early-onset group and late-onset group; 70 healthy subjects were included as controls. Genomic DNA from 70 normal controls and from those of PD patients were extracted from peripheral blood leukocytes by using standard procedures. Mutations of parkin gene (exon 1-12) in all the subjects were screened by PCR-single strand conformation polymorphism (SSCP). and further sequencing was performed in the samples with abnormal SSCP results, in order to confirm the mutation and its location. A new missense mutation Gly284Arg in a patient and 3 abnormal bands in SSCP electrophoresis from samples of another 3 patients were found. All the DNA variants were sourced from the samples of the patients with early-onset PD. It was concluded that Parkin point mutation also partially contributes to the development of early-onset Parkinson's disease in Chinese.

关键词： Parkinson's disease parkin gene point mutation

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直接植块法构建人胚雪旺细胞人工神经

复旦学报（医学版） 2004年第5期31卷 512-514,F003页

作者：吴志伟陈峥嵘汪洋程飚复旦大学附属中山医院骨科上海医学院解剖与组织胚胎学系上海200032

目的　探索人组织工程神经的构造方法。方法　应用直接植块法将人胚雪旺细胞种植于鼠尾胶包埋的聚羟基乙酸和聚乳酸共聚物支架上 ,观察细胞在支架上的吸附迁移和生长情况。结果　直接植块法能在较短时间内使人胚雪旺细胞长满支架表面 ,... 详细信息

目的　探索人组织工程神经的构造方法。方法　应用直接植块法将人胚雪旺细胞种植于鼠尾胶包埋的聚羟基乙酸和聚乳酸共聚物支架上 ,观察细胞在支架上的吸附迁移和生长情况。结果　直接植块法能在较短时间内使人胚雪旺细胞长满支架表面 ,且保持良好的功能状态。

关键词：雪旺细胞人胚人工神经支架组织工程功能状态法能构建包埋聚羟基乙酸

Depression in Patients with Parkinson’s Disease and the Associated Features

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Journal of Huazhong university of Science and Technology(medical Sciences) 2009年第6期29卷 725-728页

作者：郑瑾孙圣刚乔娴刘郁东 department of neurology Union Hospital Tongji Medical College Huazhong University of Science and Technology

The study was aimed to examine the prevalence of depression in patients with Parkinson’s disease （PD） and identify its features. A total of 131 out-patients, diagnosed as having idiopathic PD in accordance with the United Kingdom Parkinson’s Disease Society Brain Bank criteria, were interviewed with questionnaire and evaluated by Mini-Mental State Examination （MMSE）, Unified Parkinson’s Disease Rating Scale （UPDRS）, Hohen ＆Yahr staging （H＆Y staging） and Hamilton Rating Scale for Depression （HRSD）. Patients were divided into three groups in terms of HRSD score： depression group, sub-threshold depression group and non-depression group. The clinical variables and symptom profiles were obtained and compared among the three groups. The results showed that 27 patients （20.6%） fell into the depression group, 71 （54.2%） into the sub-threshold depression group, and 33 （25.2%） into the non-depression group. There were no differences in age, gender or tremor score among the groups （P〉0.05）. Significant differences were found in duration of PD, UPDRS score, rigidity score and H＆Y stage between the sub-threshold depression group （or the depression group） and the non-depression group （P〈0.05）. Moreover, the clinical variables in the subthreshold depression group had the trend of increasing with the severity of PD and their values were similar to those in the depression group. Anhedonia, feeling of incapability, sleep disturbance, gastrointestinal symptoms and depressive moods were most common in the depression group. And these symptoms also were more common in the other two groups. It is concluded that depression and sub-threshold depression are common in PD and share similar clinical features. Furthermore, subthreshold depression might be the prodrome of depression and may develop into depression as the condition progresses.

关键词： Parkinson’s disease depression subthreshold depression

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颅内脂肪瘤的CT和MRI诊断

医学影像学杂志 2001年第4期11卷 218-220页

作者：徐文坚张云亭刘松龄吴恩惠青岛大学医学院附属医院放射科山东青岛266003 天津医科大学总医院放射科天津300052

目的 :研究颅内脂肪瘤的分布特征、CT和MRI表现及诊断价值。方法 :16例颅内脂肪瘤 ,男 9例 ,女 7例 ,平均年龄 37.6岁。结果 :CT表现为脂肪样低密度区 (CT值 :- 44～ - 71HU) ,MRI表现为短T1中等T2 和高质子密度信号 ,在各序列上与脂肪... 详细信息

目的 :研究颅内脂肪瘤的分布特征、CT和MRI表现及诊断价值。方法 :16例颅内脂肪瘤 ,男 9例 ,女 7例 ,平均年龄 37.6岁。结果 :CT表现为脂肪样低密度区 (CT值 :- 44～ - 71HU) ,MRI表现为短T1中等T2 和高质子密度信号 ,在各序列上与脂肪组织信号同步。 11例单发 ,5例多发。主要分布在中线及旁中线区 ,其中 9例 (5 6 .2 5 % )为胼胝体区。 11例 (6 8.75 % )合并有颅内、外的单或多发异常 ,其中 9例 (5 6 .2 5 % )为胼低体发育异常。结论 :颅内脂肪瘤好发于颅中线区及其邻近部位 ,CT及MRI有特征性表现 ,常合并颅内、外多发异常 ,结合CT及MRI检查可诊断。

关键词：颅内脂肪瘤 X线计算机体层摄影术磁共振成像诊断 CT MRI

High glucose exacerbates β-amyloid production and the beneficial role of benfotiamine in vitro

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High glucose exacerbates β-amyloid production and the benef...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xiao-Jing SUN,Lei ZHAO,Na ZHAO,Chun-Jiu ZHONG~* department of neurology,Zhongshan hospital & Shanghai medical college State Key Laboratory of medical Neurobiology Institute of Brain Science Fudan university.

＜正＞Our previous study has demonstrated that benfotiamine,a protective agent for diabetes complications, significantly decreases p-amyloid（Aβ） production through enhancing the phosphorylation levels and reduced enzymatic activities of both GSK-3αand GSK-3βin the APP/PS1 transgenic *** evidence from a population- based study also shows a link between Alzheimer’s disease（AD） and diabetes mellitus（DM）.The prevelance rate of AD is high as much as 2 to 5 times in population suffering from DM compared with non-DM control subjects. Experimental investigations suggest that the abnormality of cerebral glucose metabolism is the key etiological factor for *** we explored the effect of benfotiamine on high glucose exacerbating Aβproduction and the possible mechanism using HEK293 APPsw overexpression *** results showed that Aβproduction was first rising and then dropping along with the increased concentrations of glucose in the cell culture medium（1 g/L,3 g/L,4.5 g/ L,5.5 g/L,6.5 g/L,7.5 g/L,8.5 g/L,10.5 g/L） and the prolongation of incubated time（6,12,and 24 h）.After being incubated for 6 h or 12 h,the Aβproduction of cells incubated in 5.5 g/L glucose was higher significantly compared with those of cells in 1 g/L glucose（6 h,P＜0.05;12 h,P＜0.01）.It was associated with high glucose up-regulating the activities of GSK-3:The ratios of p-GSK-3a/GSK-3a and p-GSK-3β/GSK-3βin 5.5 g/L group were significantly decreased（p-GSK-3α/GSK-3α,P＜0.01;p-GSK-3β/GSK-3β,P＜0.05）,and the activities of GSK-3a and GSK- 3βwere significantly increased compared with those in 1.0 g/L group（P＜0.05）.Benfotiamine significantly reduced Aβproduction enhanced by high glucose compared with control group（20 ug/mL group P＜0.05,40μg/mL group P＜0.01）.The mechanisms may be through modulating GSK-3 activities:Benfotiamine（40μg/mL） significantly decreased the activities of GSK-3a and GSK-3βcompared with control group（P＜0.05）.Increased Aβproduction induced by high glucose is associated with the up-regula

关键词： Alzheimer’s disease diabetes mellitus benfotiamene glycogen synthase kinase-3

Induction of bone marrow stromal cells into neuron-like cells by vascular endothelial growth factor

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Induction of bone marrow stromal cells into neuron-like cell...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Qiao-Yun LIU Li YI Jin-Ling HAN Ye jING Fang-Ting ZHANG Guang-Hui CUI 1 medical college of Shantou university Shantou 515041 china. 2 department of neurology Peking university Shenzhen hospital Shenzhen 518036 china 3 Central Laboratory Peking university Shenzhen hospital Shenzhen 518036 china 4 Laboratory of Male Reproductive Medicine Peking university Shenzhen hospital Shenzhen 518036 china

＜正＞Objective To investigate the possibility of induction of bone marrow stromal cells（BMSCs） into neuron-like cells by vascular endothelial growth factor（VEGF） in vitro,so as to provide experimental basis for the treatment of brain injury by cell transplantation and *** Bone marrow stromal cells were harvested from young Sprague-Dawley rats and cultured in DMEM/F12 suppplemented with 10%fetal bovine *** BMSCs were passaged 3 times and characterized by flow *** BMSCs were pre-induced by basic fibroblast growth factor（b-FGF, 10ng/ml） for 24h,then they were treated with different concentrations of vascular endothelial growth factor（VEGF）,which was used to induce BMSCs into neuron-like *** experiment was separated into four groups:Group A（contains 5ng/ mlVEGF）,Group B（contains 10ng/miVEGF）,Group C（contains 20ng/mlVEGF） and Group D served as a control group never adding *** contrast microscope was used to observe the differentiation of BMSCs daily,and the total number of neuron-like cells in 20 random fields（×200） was count at 3 and 10 days after *** technique was used to identify the differentiated BMSCs with neuron specific enolase（NSE）.Result The cultured BMSCs were CD90 positive and CD45 negative on flow *** 3 days after induction,some BMSCs in groups A～C were observed to change the shapes:cell bodies appeared refractile,cone-like,or spherical with primary and secondary *** 10 days after induction,the number of the cells which assumed neuronal morphology increased in groups A～*** in morphology of a few of BMSCs in group D was *** were the most neuron-like cells in group B,P＜0.05 for group B versus other *** were the fewest neuron-like cells in group D,P＜0.05 for group D versus other *** the neuron-like cells at 10 days after induction was more than those at 3 days after induction in all the groups,P＜0.05 for the numbers of the neuron-like ce

关键词： vascular endothelial growth factor bone marrow stromal cells neuron-like cells cell differentiation

Novel mutations of the FRMD7 gene in X-Linked congenital motor nystagmus

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Novel mutations of the FRMD7 gene in X-Linked congenital mot...

2007年浙江省神经病学学术年会

作者： BR Zhang~1 Z Liu~1 G Zhao~1 X Xie~2 X Yin~1 Z Hu~3 S Xu~1 Q Li~3 F Song~1 J Tian~1 W Luo~1 M Ding~1 K Xia~3 J Xia~3 ~1 department of neurology, Second affiliated hospital, college of Medicine, Zhcjiang university, Hangzhou, china ~2 department of Ophthalmology, Second affiliated hospital, college of Medicine, Zhejiang university, Hangzhou, china ~3 National Laboratory of medical Genetics of china,Central South university,Changsha,Hunan,china

Congenital motor nystagmus(CMN)is a relatively common oculomotor disorder characterized by bilateral uncontrollable ocular ***,the FRMD7 gene mutation has been identified as the genetic cause of *** clinical data and the genomic DNA of three Chinese CMN families were collected afcer the consent *** by two-point linkage analysis combined with haplotype analysis was performed and mutation screening was conducted in the FRMD7 gene by direct sequencing.A maximum two-point Lod score of 4.27 at θ=0.00 was obtained with marker DXS8041 in close proximity to the FRMD7 gene on chromosome *** screening in FRMD7 identified two novel missense mutations(781C>G,886G>C)and one reported nonsense mutation(1003C>T).These nucleotide alterations were neither seen in unaffected members of the families nor in 100 unrelated control *** study widens the mutation spectrum of the FRMD7 gene and it is the first report of the FRMD7 gene mutations in Chinese population.

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