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Genetic polymorphism and mRNA levels of cytochrome P450ⅡE1 and glutathione S-transferase P1 in patients with alcoholic liver disease in different nationalities

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Hepatobiliary & Pancreatic Diseases International 2009年第2期8卷 162-167页

作者： Ying Liu,Xiang-Wei Meng,Ling-Yun Zhou,Pei-Yi Zhang,Xun Sun and Ping Zhang department of Gastroenterology,First Affiliated Hospital,Jilin university,Changchun 130021,china department of Gastroenterology,Heilongjiang Province Hospital,harbin 150036,china department of medical molecular biology,harbin medical university,harbin 150086,china department of Gastroenterology First Affiliated Hospital Jilin University Changchun 130021 China.

BACKGROUND:Alcohol abuse and dependence are major factors in the pathogenesis of alcoholic liver disease(ALD).Alcohol abuse is becoming an increasingly severe problem among the Han,Mongol,and Korean nationalities in northeast *** study aimed to investigate the relationship between ALD and the genetic polymorphism and expression levels of two enzymes,cytochrome P450ⅡE1(CYPⅡE1)and glutathione S-transferase P1(GSTP1)in patients of three ***:Peripheral blood was collected from 353 Chinese patients with ALD,300 alcohol dependent patients without liver disease(alcoholic),and 360 healthy *** group included patients from the Han,Mongol and Korean ***-time polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP)were ***:Regardless of nationality,patients who carried the rare CYPⅡE1 C2 and GSTP1 Val alleles were at higher risk of *** frequency of C2 and Val in patients with ALD was respectively 50.00%and 26.98%in the Han,31.36%and 22.87%in the Mongol,and 45.87%and 22.02% in the Korean *** significant differences were seen in the frequency of either C2 or Val alleles in ALD patients among the three *** each nationality,the frequency of both C2 and Val alleles was significantly higher in ALD compared to alcoholic and healthy *** for nationality,the average mRNA levels of CYPⅡ E1 in ALD patients and healthy controls were 10.05%and 2.21%,*** average mRNA levels of GSTP1 in ALD patients and healthy controls were 0.53%and 2.12%,*** mRNA level of CYPⅡE1 was higher,and that of GSTP1 was lower in patients with ALD compared to the ***:Except for nationality,patients with ALD in this series tended to have a higher mRNA expression of CYPⅡE1 and to carry the C2 allele,and tended to have a lower mRNA expression of GSTP1 and to carry the Val *** is a causal relationship between the polymorphic alleles,which lea

关键词： alcoholic liver disease alcoholic genetic polymorphism mRNA expression

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Detecting robust gene signature through integrated analysis of multiple types of high-throughput data in liver cancer

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Acta Pharmacologica Sinica 2007年第12期28卷 2005-2010页

作者： Xin-yu ZHANG~(2,3,4,6),Tian-tian LI~(5,6),Xiang-jun LIU~(2,3,4,7)~2 department of Biological Science and Biotechnology,~3 School of Biomedicine and ~4 Ministry of Education Key Laboratory of Bioinformatics,Tsinghua university Beijing 100084,china ~5 Laboratory of medical Genetics,department of biology,harbin medical university,harbin150086.china department of Biological Science and Biotechnology Tsinghua University Beijing China School of Biomedicine Tsinghua University Beijing China Ministry of Education Key Laboratory of Bioinformatics Tsinghua University Beijing China department of biology Laboratory of Medical Genetics Harbin Medical University Harbin China

Aim:To investigate the robust gene signature in liver cancer,we applied anintegrated approach to perform a joint analysis of a highly diverse collection ofliver cancer genome-wide datasets,including genomic alterations and transcrip-tion ***:1-class Significance Analysis of Microarrays coupled withranking score method were used to identify the robust gene signature in livertumor ***:In total,1 625 051 gene expression measurements from 16public microarrays,2 pairs of serial analyses of gene expression experiments,and252 loss of heterozygosity reports obtained from 568 publications were used inthis integrated *** resulting robust gene signatures included 90 genes,which may be of great importance to liver cancer research.A system assessmentanalysis revealed that our integrative method had an accuracy of 92% and acorrelation coefficient value of ***:The system assessment resultsindicated that our method had the ability of integrating the datasets from varioustypes of sources,and eliciting more accurate results,as can be very useful in thestudy of liver cancer.

关键词： liver cancer integrated analysis robust gene signature gene expression map microarray SAGE

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Experimental Study of Rat Beta Islet Cells Cultured under Simulated Microgravity Conditions

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 47-50页

作者： Chun SONG, Xiu-Qing DUAN, Xi LI, Li-Ou HAN, Ping XU, Chun-Fang SONG, and Lian-Hong JIN (Key Laboratory of Cell Transplantation of Ministry of Health, First Affiliated Hospital of harbin medical university, harbin 150001, china department of General Surgery, First Affiliated Hospital of harbin medical university, harbin 150001, china National Key Laboratory of harbin Veterinary Research Institute of Chinese Agriculture Academy, harbin 150001, china Tissue Engineering Research Center, Histology and Embryology Staff Room, harbin medical university, harbin 150086, china ) Key Laboratory of Cell Transplantation of Ministry of Health First Affiliated Hospital of Harbin Medical University Harbin 150001 China department of General Surgery First Affiliated Hospital of Harbin Medical University Harbin 150001 China National Key Laboratory of harbin Veterinary Research Institute of Chinese Agriculture Academy harbin 150001 China Tissue Engineering Research Center Histology and Embryology Staff Room Harbin Medical University Harbin 150086 China

To observe the effects of simulated microgravity on beta islet cell culture, we have compared the survival rates and the insulin levels of the isolated rat islet cells cultured at micro- and normal gravity conditions. The survival rates of the cells cultured were determined by acridine orange-propidium iodide double-staining on day 3, 7 and 14. The morphology of the cells was observed by electron microscopy. Insulin levels were measured by radio immuno assays. Our results show that the cell number cultured under the microgravity condition is significantly higher than that under the routine condition (P<0.01). Some tubular structure shown by transmission electron microscopy, possibly for the transport of nutrients, were formed intercellularly in the microgravity cultured group on day 7. There were also abundant secretion particles and mitochondria in the cytoplasm of the cells. Scanning electron microscopy showed that there were holes formed between each islet, possibly connecting with the nutrient trans

关键词： simulated microgravity islet cell electron microscopy radioimmunoassay

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Changes in soluble interleukin-2 receptor level in serum and Na^+ -K^+ -exchanging ATPase activity in semen of infertile men caused by antisperm antibody

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Asian Journal of Andrology 2000年第2期2卷 151-153页

作者： Jiang NI Qing-Lei LI Wei ZHANG Jian-Song XIE Shu-Ling BIAN department of Physiology, harbin medical university, harbin 150086, china department of Physiology Harbin Medical University Harbin 150086 China

Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPase activi-ty in semen by phosphorus (Pi) assay. Results: The slL-2R level in serum was significantly higher and the Na^+ -K^+ -exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with thecontrols. Conclusion: The AsAb titer varies with the slL-2R level in serum. A decrease in Na^+ -K^+ -exchangingATPase activity in semen may play a role in male infertility caused by AsAb.

关键词： male infertility antisperm antibodies soluble interleukin-2 receptor Na^+-K^+-exchanging ATPase

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Decreases of voltage-dependent K~+ currents densities in ventricular myocytes of guinea pigs by chronic oxidant stress

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Acta Pharmacologica Sinica 2004年第6期25卷 49-53页

作者： De-li DONG, Yan LIU, Yu-hong ZHOU,Wei-hua SONG, He WANG, Bao-feng YANG2 department ofpharmacology, harbin medical university, harbin 150086, china department of pharmacology Harbin Medical University Harbin 150086

AIM: To determine the changes of delayed rectifier K+ currents (Ik) and inward rectifier K+ currents (Ik1) in the ventricular myocytes of guinea pigs during the gradual apoptotic process by the chronic oxidant stress treatment. METHODS: H2O250 μmol/L (24 h) was used for inducing apoptosis in the cardiomyocytes culture of neonatal rats and to treat the isolated ventricular myocytes of adult guinea pigs in vitro for 24 h. Apoptosis was evaluated by TUNEL methods and voltage-dependent K+ currents were recorded by patch-clamp techniques. RESULTS: H2O2 50 μmol/L (24 h)induced cell apoptosis in the cardiomyocytes culture of neonatal rats. This concentration was used to treat the isolated ventricular myocytes of adult guinea pigs in vitro for 24 h and the voltage-dependent K+ currents densities (Ik, Ik1) were down-regulated. The densities of the delayed rectifier K+ currents (Ik) in 50 μmol/L H2O2 group were 2.52±0.57 pA/pF vs 5.73±1.84 pA/pF in the control group at +50 mV (n=8, P<0.01). The densities of the inward rectifier K+ currents (Ik1) in 50 μmol/L H2O2 group were -13.9± 2.70 pA/pF, 2.52±0.57 pA/pF vs -59.7±11.9 pA/pF, 5.73±1.84 pA/pF in the control group at -120 mV (n=8, P< 0.01) and -40 mV (n=8, P<0.05), respectively. The extent of inward rectifier property of Ik1 was weakened by 50 μmol/L H2O2treatment. CONCLUSION: The densities of Ik, Ik1 in the cardiomyocytes of guinea pigs were down- regulated and the inward rectifier property of Ik1 was weakened during the gradual apoptotic process after 50 μmol/L H2O2 treatment for 24 h.

关键词： hydrogen peroxide potassium apoptosis cardiac myocytes

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Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats

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World Journal of Gastroenterology 2010年第48期16卷 6111-6118页

作者： Ji-Wu Han,department of Gastroenterology,The 4th Hospital of harbin medical university,harbin 150001,Heilongjiang Province,china Xiao-Rong Zhan,Xin-Yu Li,Bing Xia,Yue-Ying Wang,Jing Zhang,department of Endocrinology,First Hospital of harbin medical university,harbin 150001,Heilongjiang Province,china Bao-Xin Li,department of Pharmacology,State Key Laboratory of Biomedicine and Pharmacology,harbin medical university,harbin 150001,Heilongjiang Province,china department of Endocrinology First Hospital of Harbin Medical University Harbin 150001 Heilongjiang Province China.

AIM:To determine whether mitochondrial dysfunction resulting from high-fat diet is related to impairment of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt,also known as PKB) pathway. METHODS:Rat models of nonalcoholic fatty liver were established by high-fat diet feeding. The expression of total and phosphorylated P13K and Akt proteins in hepatocytes was determined by Western blotting. Degree of fat accumulation in liver was measured by hepatic triglyceride. Mitochondrial number and size were determined using quantitative morphometric analysis under transmission electron microscope. The permeability of the outer mitochondrial membrane was assessed by determining the potential gradient across this ***:After Wistar rats were fed with high-fat diet for 16 wk,their hepatocytes displayed an accumulation of fat (103.1 ± 12.6 vs 421.5 ± 19.7,P < 0.01),deformed mitochondria (9.0% ± 4.3% vs 83.0% ± 10.9%,P < 0.05),and a reduction in the mitochondrial membrane potential (389.385% ± 18.612% vs 249.121% ± 13.526%,P < 0.05). In addition,the expression of the phosphorylated P13K and Akt proteins in hepatocytes was reduced,as was the expression of the anti-apoptotic protein Bcl-2,while expression of the pro-apoptotic protein caspase-3 was increased. When animals were treated with pharmacological inhibitors of P13K or Akt,instead of high-fat diet,a similar pattern of hepatocellular fat accumulation,mitochondrial impairment,and change in the levels of PI3K,Akt,Bcl-2 was observed. CONCLUSION:High-fat diet appears to inhibit the PI3K/Akt signaling pathway,which may lead to hepa-tocellular injury through activation of the mitochondrial membrane pathway of apoptosis.

关键词： Nonalcoholic fatty liver Phosphatidylinositol 3-kinase/protein kinase B signaling pathway Mitochondria B-cell lymphoma gene 2 Caspase-3

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Efficacy and factors influencing treatment with peginterferon alpha-2a and ribavirin in elderly patients with chronic hepatitis C

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Hepatobiliary & Pancreatic Diseases International 2012年第2期11卷 185-192页

作者： Jian-Wu Yu,Li-Jie Sun,Peng Kang,Bing-Zhu Yan and Yong-Hua Zhao department of Infectious Diseases, Second Affiliated Hospital, harbin medical university, harbin 150086, china department of Infectious Diseases Second Affiliated Hospital Harbin Medical University Harbin 150086 China

BACKGROUND:In china,hepatitis C virus(HCV) infection is characterized by an increasing prevalence during *** study was undertaken to evaluate the efficacy of treatment with peginterferon alpha-2a and ribavirin in elderly chronic hepatitis C(CHC) patients and study the factors related to the sustained virologic response(SVR).METHODS:The medical records of 417 patients treated with peginterferon and ribavirin were retrospectively *** patients were divided into two groups according to age:patients aged ≥65 years(n=140) and patients aged <65 years(n=277).The rate of ribavirin reduction or discontinuation and virologic response rates of the two groups were *** factors influencing SVR were studied by multivariate ***:Ribavirin reduction or discontinuation was more frequent in patients aged ≥65 years than patients aged <65 years(37.1%,52/140 vs 20.2%,56/277;χ 2 =13.883,P<0.001).For genotype 1,patients aged ≥65 years had a higher relapse rate(50.0%,42/84 vs 29.2%,52/178;χ 2 =10.718,P=0.001) and a lower SVR rate(40.0%,42/105 vs 60.0%,126/210;χ 2 =11.250,P=0.001) than patients aged <65 *** were no significant differences in virologic response rates between the two groups for patients with genotype *** genotype 1,in patients aged ≥65 years,the SVR rate of females was lower than that of males(28.6%,12/42 vs 47.6%,30/63;χ 2 =8.150,P=0.004);in the high viral load group,patients aged ≥65 years had a lower SVR rate than patients aged <65 years(30.0%,18/60 vs 54.8%,69/126;χ 2 =10.010,P=0.002).In multivariate logistic regression analysis,the independent factors associated with SVR in patients aged ≥65 years were sex(P=0.020),genotype(P=0.005),ribavirin reduction or discontinuation(P=0.009) and presence of rapid virologic response(RVR)(P=0.001).CONCLUSIONS:The rate of ribavirin reduction or discontinuation and relapse rate of patients aged ≥65 years with genotype 1 are high,and the SVR rate is *** has no impact on virologic respons

关键词： chronic hepatitis C elderly patients sustained virologic response

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Cell cycle-dependent gene networks relevant to cancer

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Progress in Natural Science:Materials International 2008年第8期18卷 945-952页

作者： Yun Xiao a,1, Xia Lia,b,*,1, Yueying Yang a, Yadong Wangc, Shaoqi Rao a,b a College of Bioinformatics Science and Technology, and Bio-pharmaceutical Key Laboratory of Heilongjiang Province and State, harbin medical university, harbin 150086, china b Biomedical Engineering Institute of Capital medical university, Beijing 10004, china c department of Computer Science, harbin Institute of Technology, harbin 150080, china College of Bioinformatics Science and Technology and Bio-pharmaceutical Key Laboratory of Heilongjiang Province and State Harbin Medical University Harbin 150086 China Biomedical Engineering Institute of Capital medical university Beijing 10004 China department of Computer Science Harbin Institute of Technology Harbin 150080 China

The analysis of sophisticated interplays between cell cycle-dependent genes in a disease condition is one of the largely unexplored areas in modern tumor biology research. Many cell cycle-dependent genes are either oncogenes or suppressor genes, or are closely associated with the transition of a cell cycle. However, it is unclear how the complicated relationships between these cell cycle-dependent genes are, especially in cancers. Here, we sought to identify significant expression relationships between cell cycle-dependent genes by analyzing a HeLa microarray dataset using a local alignment algorithm and constructed a gene transcriptional network specific to the cancer by assembling these newly identified gene-gene relationships. We further characterized this global network by partitioning the whole network into several cell cycle phase-specific sub-networks. All generated networks exhibited the power-law node-degree distribution, and the average clustering coefficients of these networks were remarkably higher than those of pure scale-free networks, indicating a property of hierarchical modularity. Based on the known protein-protein interactions and Gene Ontology annotation data, the proteins encoded by cell cycle-dependent interacting genes tended to share the same biological functions or to be involved in the same biological processes, rather than interacting by physical means. Finally, we identified the hub genes related to cancer based on the topological importance that maintain the basic structure of cell cycle-dependent gene networks.

关键词： Cell cycle Microarray HeLa cell Gene network

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Advances in gene therapy of liver cirrhosis: a review

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World Journal of Gastroenterology 2001年第1期7卷 1-8页

作者： Wen Jie Dai Hong Chi Jiang Second department of General Surgery, the First Clinical School, harbin medical university, harbin 150001, Heilongjiang Province, china Second department of General Surgery the First Clinical School Harbin Medical University Harbin 150001 Heilongjiang Province China. wenjiedai@***

INTRODUCTIONLiver fibrosis or cirrhosis is a common progressively pathological lesion of chronic liver diseases in response to various liver-damaging factors. The main mechanisms of fibrotic or cirrhotic initiation an... 详细信息

关键词： Gene Therapy Humans Liver Cirrhosis Telomere

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microRNA-181b targets MLK2 in HL-60 cells

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Science china(Life Sciences) 2010年第1期53卷 101-106页

作者： CHEN Hong1,CHEN Qun2,FANG Ming3 & MI Yan1 1 The First Affiliated Hospital of harbin medical university,harbin 150101,china 2 harbin Acheng District People’s Hospital,harbin 150300,china 3 Graduate department of harbin medical university,harbin 150001,china The First Affiliated Hospital of harbin medical university Harbin China harbin Acheng District People’s Hospital Harbin China Graduate department of harbin medical university Harbin China

microRNAs(miRNAs) play critical roles in many different cellular processes,including metabolism,apoptosis,differentiation and *** showed miR-181b to be highly expressed in acute myeloid leukemia(AML).Furthermore,miR-181b contributed to proliferation of AML cells by targeting *** results demonstrated that miR-181b plays an important role in the biology of AML and may be useful in developing therapies targeting miRNAs.

关键词： microRNA-181b MLK2 AML HL-60 proliferation apoptosis

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