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超声波对小白鼠生精上皮的影响

四川解剖学杂志 1995年第1期3卷 7-9页

作者：薛庆善袁仕取中山医科大学组织学与胚胎学教研室陕西师范大学生物系

用功率为５Ｗ／ｃｍ２、频率为１．１０ＭＨｚ的超声波照射小白鼠睾丸５分钟（实验组１）、１０分钟（实验组Ⅱ），分别于处理后２４小时，４８小时及７天时间切取睾丸组织，制作石蜡切片，在光镜下观察生精上皮的组织学变化并与对照组... 详细信息

用功率为５Ｗ／ｃｍ２、频率为１．１０ＭＨｚ的超声波照射小白鼠睾丸５分钟（实验组１）、１０分钟（实验组Ⅱ），分别于处理后２４小时，４８小时及７天时间切取睾丸组织，制作石蜡切片，在光镜下观察生精上皮的组织学变化并与对照组进行比较。结果显示：（１）小白鼠睾丸经超声波照射后，曲细精管萎缩，管径变小；生精上皮变薄，精子发生时相消失；生精细胞减少，没有精子形成。（２）超声波照射１０分钟对生精上皮的损伤比照射５分钟更为严重。（３）超声波照射后２４小时，生精上皮即受到破坏，精子细胞减少；照射后４８小时，上皮受损伤的程度增大；照射后７天时间，曲细精管的组织学结构开始恢复，但仍无精子形成。（４）超声波对生精上皮的影响主要限于精母细胞、精子细胞和精子，而精原细胞与支持细胞没有明显变化。上述结果表明，超声波能够抑制小白鼠的精子发生，该抑制作用可能可逆。

关键词：精子发生形态学超声波避孕

Histologic research of microcirculation patterns in human and xengraft model glioblastoma

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Histologic research of microcirculation patterns in human an...

中国抗癌协会神经肿瘤专业委员会第八届学术会议

作者： department of Neurosurgery, The First Affiliated Hospital, china medical university, Shenyang, Liaoning Province 110001, People’s Republic of china (Yin-Sheng Chen*, Zhi-Tao Jing, and Yun-Jie Wang) State Key Laboratory for Cancer Research in southern china and department of Neurosurgery/Neuro-oncology, Cancer Center, Sun Yat-sen university, guangzhou, Guangdong Province 510060, People’s Republic of china (Yin-Sheng Chen*, Cong Li and Zhong-Ping Chen)

Glioblastoma is one of the most angiogenic human tumors and characterized by microvascular proliferations. A better understanding of glioblastoma vasculature is needed to optimize anti-angiogenic therapy that has shown a promising but incomplet efficacy. The present study examined 48 glioblastomas by CD34 endothelial marker periodic acid-Schiff （PAS） dual staining and found there were four types of microcirculation pattern in human glioblastomas: endothelium dependent vessels, tumor cell dependent vessels, extracellular matrix （ECM） dependent vessels, and mosaic vessels. We confirmed subsequently that these four types of blood supply sources for tumors also exist in xengraft model glioblastomas. Here, we described and sorted the microcirculation pattern in human glioblastomas systematically according to histologic evidences and open a new perspective for the anti-vascular treatment strategy.

关键词： glioblastoma angiogenesis histology dual staining

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同心圆测试格在形态计量分析中的应用

四川解剖学杂志 1995年第2期3卷 65-69页

作者：薛庆善肖渝平冯武鸣中山医科大学组织学与胚胎学教研室陕西师范大学生物系

本文提出了一种利用同心圆测试格定量描述生物组织成分呈辐射状分布时结构特征的形态计量学方法。

关键词：辐射分布同心圆测试格形态计量学

低能量氦氖激光照射促进体外培养神经元的突起生长和 RNA 合成

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解剖学报 1989年第3期20卷 302-305页

作者：周汉城郭畹华曾园山陈其辉中山医科大学组织学胚胎学教研室广州510089

机械分离新生大白鼠颈上节细胞,进行分离细胞培养,培养物分为激光照射组与对照组。两组均在含有神经生长因子(NGF)的培养液中生长。激光照射组每隔2小时用4mW 氦氖激光照射1次,每次5分钟,直至终止培养。对照组未经激光照射。两组培养物... 详细信息

机械分离新生大白鼠颈上节细胞,进行分离细胞培养,培养物分为激光照射组与对照组。两组均在含有神经生长因子(NGF)的培养液中生长。激光照射组每隔2小时用4mW 氦氖激光照射1次,每次5分钟,直至终止培养。对照组未经激光照射。两组培养物分别培养20、22、24和28小时,测量培养物的神经元突起生长;在培养22小时,用~sH-尿嘧啶核苷和~3H-胸腺嘧啶核苷标记培养物的细胞,放射自显影法观察神经元的 DNA 和 RNA 合成。实验结果表明,低能量氦氖激光照射能促进体外培养的神经元神经突起的生长,促进胞质 RNA 的合成,但未见合成 DNA增进。

关键词：激光细胞培养 NGF RNA 大鼠

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安氟醚对离体鼠心的心肌保护作用

广州医药 2000年第5期31卷 13-15页

作者：程贵蓉施娣陶圣祥易胜中武汉科技大学医学院组胚教研室 430081 湖北医科大学武汉430071

目的 :探讨安氟醚 (enflurane ,EF)对离体缺血再灌注 (Ischemic/reperfusion,I/R)鼠心模型有氧代谢的影响及与缺血预处理 (Ischemicpreconditioning,IPC)比较。方法 :分 4组 ,对照组、缺血再灌注组、安氟醚组、缺血预处理组 ,离体I/R鼠... 详细信息

目的 :探讨安氟醚 (enflurane ,EF)对离体缺血再灌注 (Ischemic/reperfusion,I/R)鼠心模型有氧代谢的影响及与缺血预处理 (Ischemicpreconditioning,IPC)比较。方法 :分 4组 ,对照组、缺血再灌注组、安氟醚组、缺血预处理组 ,离体I/R鼠心采用主动脉根部顺行恒温灌注 ,观察心肌超微结构、CAT和CCO活性。结果 :缺血再灌注组的超微结构损伤严重 ,而安氟醚组、缺血预处理组超微结构基本完整 :从CAT和CCO活性方面 ,同安氟醚组、缺血预处理组相比 ,缺血再灌注组损伤严重 (P 0 0 5 )。结论 :安氟醚能改善离体I/R鼠心肌有氧代谢及抗氧自由基的作用 ,因此对心肌有保护作用。

关键词：缺血预处理心肌保护安氟醚心肌再灌注损伤

Expert Consensus on Pathological Diagnosis of Intrahepatic Cholangiocarcinoma(2022 version)

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Journal of Clinical and Translational Hepatology 2023年第7期11卷 1553-1564页

作者： Han Wang Jun Chen Xin Zhang Xia Sheng Xiao-Yan Chang Jie Chen Min-Shan Chen Hui Dong Guang-Jie Duan He-Ping Hu Zhi-Yong Huang Wei-Dong Jia Xiao-Qing Jiang Dong Kuang Shan-Shan Li Zeng-Shan Li Chang-Li Lu Shu-Kui Qin Xue-Shan Qiu Li-Juan Qu Chun-Kui Shao Feng Shen Guo-Ming Shi Su-Sheng Shi Yu-Jun Shi Hui-Chuan Sun Xiao-Dong Teng Bin Wang Zhan-Bo Wang Tian-Fu Wen Jia-Mei Yang Qiao-Qiao Yang Sheng-Long Ye Hong-Fang Yin Zhen-Gang Yuan Jing-Ping Yun Feng-Lin Zang Hong-Qi Zhang Li-Hong Zhang Jing-Min Zhao Jian Zhou Wei-Xun Zhou Jia Fan Xiao-Ping Chen Wan Yee Lau Yuan Ji Wen-Ming Cong Chinese Society of Liver Cancer of Chinese Anti-Cancer Association Digestive Disease Group of Chinese Society of Pathology,Chinese medical Association Chinese Society of Pathology of Chinese Anti-Cancer Association Hepatic Surgery Group of Chinese Society of Surgery,Chinese medical Association Biliary Tract Tumor Committee of china Anti-Cancer Association Chinese Society of Clinical Oncology Idepartment of Pathology Eastern Hepatobilary Surgery HospitalNaval Medical UniversityShanghaiChina department of Pathology the Affiliated Drum Tower HospitalNanjing University Medical SchoolNanjingJiangsuChina department of Pathology Zhongshan HospitalFudan UniversityShanghaiChina department of Pathology Minhang HospitalFudan Uni-versityShanghaiChina department of Pathology Peking Union Medical College HospitalChinese Academy of Medical Sci-ences and Peking Union Medical CollegeBeijingChina department of Liver Surgery Sun Yat-Sen University Cancer CenterGuangzhouGuangdongChina department of Pathology The First Affiliated HospitalArmy Medical UniversityChongqingChina department of Hepatobiliary Medicine Eastern Hepatobiliary Surgery HospitalNaval Medical UniversityShanghaiChina Hepatic Surgery Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWu-hanHubeiChina department of General Surgery The First Affliated Hospital of USTCDivision of Life Science and Medi-cineUniversity of Science and Technology of ChinaHefeiAnhuiChina department of Biliary Surgery I Eastern Hepatobil-iary Surgery HospitalNaval Medical UniversityShanghaiChina department of Pathology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina department of Pathology The First Afiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenanChina department of Pathology Xijing Hospi-talAir Force Medical UniversityXi'anShaanxiChina department of Pathology West China HospitalSichuan UniversityChengduSichuanChina Cancer Center of Jinling Hospital Nanjing University of Chinese MedicineNanjingJiangsuChina department of Pathology The First Affiliated HospitalChina Medical UniversityShenyangLiaoningChina department of Pathology The 900 Hospital of the Chinese People's Liberation Army Joint Logistics TeamFuzhouFujianChina department of Pathology The Third Affiliated HospitalSun Yat-Sen UniversityGuangz

Intrahepatic cholangiocarcinoma(iCCA)can originate from the large bile duct group(segment bile ducts and area bile ducts),small bile duct group(septal bile ducts and interlobular bile ducts),and terminal bile duct group(bile ductules and canals of Hering)of the intrahepatic biliary tree,which can be histopathological corresponding to large duct type iCCA,small duct type iCCA and iCCA with ductal plate malformation pattern,and cholangiolocarcinoma,*** challenge in pathological diagnosis of above subtypes of iCCA falls in the distinction of cellular morphologies,tissue structures,growth patterns,invasive behaviors,immunophenotypes,molecular mutations,and surgical *** these reasons,this expert consensus provides nine recommendations as a reference for standardizing and refining the diagnosis of pathological subtypes of iCCA,mainly based on the 5th edition of the World Health Organization Classification of Tumours of the Digestive System.

关键词： Intrahepatic cholangiocarcinoma Pathology Histological type Immunohistochemistry Molecular variation