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Donor liver natural killer cells alleviate liver allograft acute rejection in rats

Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 386-392页

作者： Jian-Dong Yu,Tian-Zhu Long,Guo-Lin Li,Li-Hong Lv,Hao-Ming Lin,Yong-Heng Huang,Ya-Jin Chen and Yun-Le Wan department of Hepatobiliary surgery,sun Yat-sen Memorial Hospital,sun Yat-sen University,guangzhou 510120,China department of breast surgery,guangzhou women and children’s medical center,guangzhou 510120,China department of Hepatobiliary surgery Sun Yat-Sen Memorial Hospital Sun Yat-Sen University Guangzhou 510120 China department of breast surgery Guangzhou Women and Children's Medical Center Guangzhou 510120 China

BACKGROUND:Liver enriched natural killer (NK) cells are of high immune ***,the function of donor liver NK cells in allogeneic liver transplantation (LTx) remains ***:Ten Gy of whole body gamma-irradiation (WBI) from a 60 Co source at 0.6 Gy/min was used for depleting donorderived leukocytes,and transfusion of purified liver NK cells isolated from the same type rat as donor (donor type liver NK cells,dtl NKs) through portal vein was performed immediately after grafting the irradiated ***-transplant survival observation on recipients and histopathological detection of liver grafts were adoptive to evaluate the biological impact of donor liver NK cells on recipients’ survival in rat ***:Transfusion of dtl NKs did not shorten the survival time among the recipients of spontaneous tolerance model (BN to LEW rat) after rat LTx,but prolonged the liver graft survival among the recipients depleted of donor-derived leukocytes in the acute rejection model (LEW to BN rat).Compared to the recipients in the groups which received the graft depleted of donor-derived leukocytes,better survival and less damage in the allografts were also found among the recipients in the two different strain combinations of liver allograft due to transfusion of dtl ***:Donor liver NK cells alone do not exacerbate liver allograft acute ***,they can alleviate it,and improve the recipients’ survival.

关键词： liver transplantation natural killer cell donor transfusion graft rejection

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Functional distinction of rat liver natural killer cells from spleen natural killer cells under normal and acidic conditions in vitro

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 285-293页

作者： Li-Hong Lv, Jian-Dong Yu, Guo-Lin Li, Tian-Zhu Long, Wei Zhang, Ya-Jin Chen, Jun Min and Yun-Le Wan department of Hepatobiliary surgery, sun Yat-sen Memorial Hospital, sun Yat-sen University department of breast surgery, guangzhou women and children’s medical center guangzhou 510120, China department of Hepatobiliary and Pancreatic surgery, First Affiliated Hospital, Zhejiang University school of Medicine, Hangzhou 310003, China department of Hepatobiliary surgery Sun Yat-Sen Memorial Hospital Sun Yat-Sen University (Lv LH Yu JD Li GL Chen YJ Min J and Wan YL) guangzhou 510120 China department of breast surgery Guangzhou Women and Children's Medical Center (Long TZ) department of Hepatobiliary and Pancreatic surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND: The microenvironment within solid tumors has often been shown to exhibit an acidic extracellular pH. Although the morphologic and functional differences in natural killer (NK) cells of the liver and spleen have been reported previously under physiological conditions, the difference under acidic conditions is still unclear. This study was to investigate the differences in the morphological and functional characteristics between rat liver and spleen NK cells under normal and acidic conditions in vitro. METHODs: Liver and spleen NK cells were isolated and purified from sprague-Dawley rats by density gradient centrifugation and the Dynabeads FlowComp TM Flexi system, and stimulated for 4 days with or without IL-2 or treated with low pH or control for different times. Morphology was examined by scanning electron microscopy (sEM) and transmission electron microscopy (TEM), cell death and proliferation assays were performed by flow cytometry, IFN-γ production was tested by ELIsA, and cytotoxic activity was evaluated by lactate dehydrogenase (LDH) release assay. REsULTs: Liver NK cells had significantly higher levels of cytotoxic activity than spleen NK cells under normal and acidic conditions, and the maximum difference was observed at pH 5.6. Further analysis revealed that the cytotoxic activity of NK cells was correlated with morphology, cell death, proliferative activity and IFN-γ production. By TEM, liver NK cells contained a greater number of electron-dense granules per cell at pH ***, a modest elevation of cell death and reduction of proliferation of liver NK cells occurred within a range of 5.6-7.2. Interestingly, an acidic extracellular pH only marginally, and not significantly, suppressed IFN-γ production by liver NK cells. CONCLUsION: The sharp morphological and functional differences shown by the two types of NK cells in vitro indicate that liver NK cells are unexpectedly resistant to pH shock.

关键词： natural killer cells liver spleen tumor microenvironment extracellular pH

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Is There A Relationship between Polycystic Ovary syndrome and Endometriosis?

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Journal of Reproduction and Contraception 2011年第3期22卷 177-182页

作者： Xue-ya QIAN Hao WU Xiao-wei XI department of Obstetrics and Gynecology Guangzhou Women and Children＇s Medical Center Guangzhou 510623 China department of Obstetrics and Gynecology Shanghai First people＇s Hospital Affiliated to Shanghai JiaoTong University Shanghai 200011 China

The endocrinologic and metabolic abnormalities of women with polycystic ovary syndrome （PCOs） can result in a series of endometrial diseases. Abnormalities of hyperandrogenism and hyperinsulinemia that may be found in PCOs can elevate the levels of E2 indirectly, reduce progesterone secretion and induce some growth factors such as vascular endothelial growth factor （VEGF） and insulin-like growth factors （IGFs） over expression, which may have a major impact on endometriosis occurrence and development. We suppose that there is a possible connection between PCOs and endometriosis.

关键词： polycystic ovary syndrome (PCOs) endometriosis (EMs) estrogen metabolism growth factors

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中国晚期三阴性乳腺癌临床诊疗指南(2024版)

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中华肿瘤杂志 2024年第6期46卷 471-480页

作者：中国抗癌协会乳腺癌专业委员会中国抗癌协会国际医疗交流分会中国医师协会肿瘤医师分会乳腺癌学组袁芃徐兵河岳健不详国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院特需医疗部北京100021 国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院药物临床试验研究中心北京100021

乳腺癌是全球女性最常见的恶性肿瘤,2020年GLOBOCAN流行病学数据显示,乳腺癌首次超过肺癌成为全球第一高发的恶性肿瘤,严重威胁女性健康。相较于欧美人群,中国女性患者的乳腺癌发病率相对较低,但是随着近年来生活方式、饮食习惯的改变等... 详细信息

乳腺癌是全球女性最常见的恶性肿瘤,2020年GLOBOCAN流行病学数据显示,乳腺癌首次超过肺癌成为全球第一高发的恶性肿瘤,严重威胁女性健康。相较于欧美人群,中国女性患者的乳腺癌发病率相对较低,但是随着近年来生活方式、饮食习惯的改变等,中国人群乳腺癌发病逐年增加。中国2020年年龄标化的乳腺癌发病率达到39.1/10万,新发乳腺癌达41.6万例,占全球每年新发乳腺癌总人数的18.4%。乳腺癌防治工作也日益严峻,其中,三阴性乳腺癌作为一种恶性程度高、预后较差的乳腺癌亚型,由于以往缺乏明确的治疗靶点,近年来治疗进展缓慢。尤其是晚期三阴性乳腺癌的治疗,由于治疗手段相对有限,有待进一步优化治疗建议。因此,在中国抗癌协会乳腺癌专业委员会、中国抗癌协会国际医疗交流分会和中国医师协会肿瘤医师分会乳腺癌学组的倡导下,中国乳腺癌各学科诊疗专家组结合国内外最新研究证据、治疗理念和经验,制定了中国晚期三阴性乳腺癌临床诊疗指南(2024版),旨在优化中国晚期三阴性乳腺癌精准治疗决策。

关键词：乳腺肿瘤晚期三阴性乳腺癌诊断治疗指南

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中国县域乳腺癌防治管理路径与质量控制指南(2023版)

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中华肿瘤杂志 2024年第1期46卷 1-18页

作者：国家肿瘤质控中心乳腺癌专家委员会健康中国研究中心癌症防治专家委员会马飞徐兵河兰波路平刘梦友不详国家癌症中心、国家肿瘤临床医学研究中心、中国医学科学院北京协和医学院肿瘤医院内科北京100021 国家癌症中心、国家肿瘤临床医学研究中心、中国医学科学院北京协和医学院肿瘤医院药物临床试验研究中心北京100021 新乡医学院第一附属医院肿瘤内科利辛县人民医院甲乳外科

乳腺癌是威胁中国女性健康的第一大恶性肿瘤。2016年中国女性新发乳腺癌30.60万例, 其中中国县域地区(包含农村地区)新发乳腺癌约10.04万例, 约占同年中国总新发乳腺癌的33%。县域(县级或县级市)医院是多数县域乳腺癌患者的首诊单位, ... 详细信息

乳腺癌是威胁中国女性健康的第一大恶性肿瘤。2016年中国女性新发乳腺癌30.60万例, 其中中国县域地区(包含农村地区)新发乳腺癌约10.04万例, 约占同年中国总新发乳腺癌的33%。县域(县级或县级市)医院是多数县域乳腺癌患者的首诊单位, 在乳腺癌的预防、筛查、维持治疗、康复随访和转诊等方面发挥重要作用。县域医院由于经济和地理等因素的限制, 在医疗设备、人才、药物可及性等方面都相对不足, 亟需适合县域实际情况的乳腺癌防治管理指南。因此, 在分级诊疗的政策背景下, 国家肿瘤质控中心乳腺癌专家委员会和健康中国研究中心癌症防治专家委员会起草并制定了《中国县域乳腺癌防治管理路径与质量控制指南(2023版)》, 旨在进一步促进优质医疗资源扩容下沉、区域均衡布局, 提高整体乳腺癌的防控效果, 改善县域乳腺癌患者的预后和生活质量。指南采用路径形式, 更加简明清晰且实操性强, 以供广大县域临床医师参考。

关键词：乳腺肿瘤防治管理质量控制县域指南

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Expression of centromere protein-C(CENP-C) in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation

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Journal of Reproduction and Contraception 2016年第1期27卷 18-24页

作者： Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU Reproductive Medicine center Children's Hospital of Shanxi & Women Health Center of Shanxi Laboratory of Genome Project Beijing Institute of Basic Medical Science department of Obstetrics and Gynecology General Hospital of Taiyuan Iron & Steel Co. Ltd Genetics Laboratory Children's Hospital of Shanxi & Women Health Center of Shanxi

Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （sA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FIsH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with sA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FIsH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to sA induced by chromosomal aneuploid.

关键词： spontaneous abortion (sA) aneuploidy centromere protein-C (CENP-C) gene expression

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Little Citizen Moralitv Construction Prevails

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women of China 2004年第6期 67-68页

作者： China National children’s center 中国儿童中心

The Little Citizen Morality Constryction Project, initiated by China National children's center (CNCC) in 2002, has been greatly supported by the Propaganda department of the Party, the All-China women's Federation (ACWF) and the Ministry of Education. The project kicked off officially at the Great Hall pf the People in 2002, and in the sybsequent two years, numerous activities were held, enabling Chinese children to become fully aware of the significance of morality construction, and more importantly, to d...

关键词：公民道德实践活动中国儿童中心

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Clinical practice guideline for breast fibroadenoma: Chinese society of breast surgery (CsBrs) practice guideline 2021

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Chinese medical Journal 2021年第9期134卷 1014-1016页

作者： Yuan Peng Fei Xie Yi Zhao shu Wang Chinese society of breast surgery department of breast center Peking University People’s HospitalBeijing 100044China department of Oncology Shengjing Hospital of China Medical UniversityShenyangLiaoning 110022China 不详

breast fibroadenoma is the most common benign tumor of the breast in women,and can occur at all ***,these tumors are more commonly seen in women aged 15 to 35 years.[1]Most fibroadenomas often undergo self-limited growth and usually stabilize after several *** clinical diagnosis is mainly based on clinical palpation and ultrasonographic examination,while the golden standard diagnosis is pathological *** standardize the clinical diagnosis and treatment of breast fibroadenoma,the Chinese society of breast surgery(CsBrs)conducted a literature review of experts’opinions,and determined the key clinical questions for the clinical practice guideline of breast *** group evaluated the relevant evidences using the grading of recommendations assessment,development,and evaluation system,and developed the clinical practice guideline for breast fibroadenoma:CsBrs practice guideline 2021,with the aim of providing clinical practice guidance to breast surgeons in China.

关键词： surgery fibroadenoma diagnosis

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多不饱和脂肪酸与肥胖

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实用儿科临床杂志 2009年第19期24卷 1522-1524页

作者：江津齐可民鞍山市妇儿医院儿内科辽宁鞍山114014 首都医科大学附属北京儿童医院北京市儿科研究所营养中心北京100045

流行病学与动物实验研究表明,膳食n-6多不饱和脂肪酸(PUFAs)摄入增加、n-3 PUFAs摄入减少或二者比例失调均与肥胖的发生相关,其作用机制涉及脂肪合成与氧化、脂肪细胞分化增殖、瘦素表达等多个方面。PUFAs对过氧化物酶增长因子活化受体... 详细信息

流行病学与动物实验研究表明,膳食n-6多不饱和脂肪酸(PUFAs)摄入增加、n-3 PUFAs摄入减少或二者比例失调均与肥胖的发生相关,其作用机制涉及脂肪合成与氧化、脂肪细胞分化增殖、瘦素表达等多个方面。PUFAs对过氧化物酶增长因子活化受体、CCAAT增强子结合蛋白等转录因子的调控是其发挥作用的分子基础。

关键词：肥胖多不饱和脂肪酸转录因子瘦素

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Hormones and cancer

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Cell Research 2007年第4期17卷 277-279页

作者： Yongfeng shang~1 ~1department of Biochemistry and Molecular Biology,Peking University Health science center,38 Xueyuan Rd,Beijing 100083,China department of Biochemistry and Molecular Biology Peking University Health Science Center Beijing China

Estrogen exhibits a broad spectrum of physiologicalfunctions ranging from regulation of the menstrual cycleand reproduction to the modulation of bone density,brainfunction,and cholesterol ***,estrogenis also associated with pathological complications par-ticularly with the onset of gynaecological malignanciesincluding breast cancer and endometrial cancer;estrogenis now considered to be a classical etiological factor forbreast cancer and endometrial *** the early 1970s,it was reported that there was a 20-35% increase in incidence of endometrial cancer in WesternCaucasian women who had undergone estrogen therapy[1].subsequently,a variety of clinical and epidemiologi-cal investigations,with support from studies in cell cultureand animal models,have demonstrated the association ofestrogen with the development and/or progression of *** 2002,the Us National Toxicology Programlisted steroidal estrogens as carcinogens for the first *** report cites data from human epidemiologic studies thatshow an association between estrogen replacement therapyand an increase in the risk of endometrial cancer,as well asa less consistent increase in the risk of breast cancer.

关键词： gene NsABP Hormones and cancer ERE sERMs

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