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Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion

Acta Pharmacologica Sinica 2004年第4期25卷 98-104页

作者： San-hua LENG, Fu-er LU2, Li-jun XU Institute of Integrated Traditional Chinese & Western Medicine, tongji Hospital, tongjimedical college, huazhong university of science & technology, wuhan 430030, china Institute of Integrated Traditional Chinese & Western Medicine Tongji Hospital Tongji Medical College Huazhong University of Science & Technology China

AIM: To explore the anti-diabetic effects of berberine and its influence on insulin secretion. METHODS: Impaired glucose tolerance rats induced by iv injection of streptozotocin 30 mg/kgwere treated with berberine 187.5 and 562.5 mg/kg while fed with high fat laboratory chow. After rats were treated for 4 weeks, oral glucose tolerance was determined, and for 8 weeks, the fasting blood glucose, insulin, lipid series were determined. In insulin secretion experiments, berberine 93.75, 187.5, and 562.5 mg/kg was administered orally to BALB/c mice at a bolus. The murine serum was collected 2 h after the berberine administration for insulin determination. Insulin released from HIT-T15 cells and pancreatic islets incubated with berberine 1-100 μmol/L for 12 h was determined. RESULTS: The levels of fasting blood glucose (7.4±1.5 or 7.3±1.3 vs 9.3±1.3 mmol/L), triglycerides (0.61±0.22 or 0.63± 0.17 vs 1.8±0.7 mmol/L), total cholesterol (1.8±0.3 or 1.9±0.3 vs 2.2±0.2 mmol/L), free fatty acid (456±93 or 460±72 vs 550±113 μmol/L) and apolipoprotein B (0.37±0.02 or 0.42±0.05 vs 0.46±0.04 g/L) were reduced greatly in berberine-treated groups at doses of 187.5 and 562.5 mg·kg-1·d-1, respectively as compared with those in control group (P<0.05 or P<0.01),whereas high densitylipoprotein-cholesterol (1.5±0.3 or 1.4±0.3vs 1.1±0.1 g/L), apolipoprotein AI (0.80±0.08 or 0.87±0.08 vs 0.71±0.06 g/L) were significantly increased (P<0.05 or P<0.01), and oral glucose tolerance was improved. In vitro experiment showed that berberine 1-10 μmol/L facilitated insulin secretion of HIT-T15 cells and murine pancreatic islets in a dose-dependent manner. Meanwhile murine serum insulin level (27.5±2.7 or 29±4 or 29±4 vs 24.3±2.8 pIU/L) was undoubtedly promoted and blood glucose (4.52±0.31 or 4.45±0.29 or 4.30±0.19 vs 4.87±0.21 mmol/L) was reduced after berberine administration at doses of 93.75, 187.5, and 562.5 mg/kg, respectively in the BALB/c mice. CONCLUSION: Berberine possesses anti-diabetic effec

关键词： berberine diabetes mellitus glucose tolerance test insulin HIT-T15 cells pancreatic islets lipids

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Immune protection of auxiliary liver to other allograft: report of 3 cases

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Hepatobiliary & Pancreatic Diseases International 2003年第3期2卷 351-353页

作者： Nian-Qiao Gong Qi-Fa Ye the Institute of Organ Transplantation, tongji Hospital, tongji medical college, huazhong university of science and technology, wuhan 430030, china Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. nqgong@***

OBJECTIVE: To assess the immune status of auxiliary liver transplantation and to clarify the immune protection of auxiliary liver to other allograft. METHODS: Immunological markers and pathological changes in 3 patients undergoing auxiliary liver transplantation were analysed. RESULTS: The lower the concentration of immunosuppressive agent, the less the rejection and the milder the intensity in the 3 patients. The function of allograft after auxiliary liver transplantation was excellent. CONCLUSIONS: Patients are in a low immune reaction state after auxiliary liver transplantation. Auxiliary liver can protect other allografts by related immunological mechanisms. The side-effects of low-concentration immunosuppressive agents on auxiliary liver and other allografts are mild.

关键词： auxiliary liver transplantation immune protection allograft

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Inhibiting Apoptosis of CTLL-2 Cells to Enhance Their GVL Effects via Anti-Fas Ribozyme

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 559-565页

作者： Min ZHANG, Fang LIU, Lin-Bo LIU, Yong YOU, Zhi-Chao CHEN, and Ping ZOUInstitute of Hematology, Union Hospital, tongji medical college, huazhong university of science & technology, wuhan 430022, china Institute of Hematology Union Hospital Tongji Medical College Huazhong University of Science & TechnologyWuhan 430022 China

To investigate the inhibition role of anti-Fas hammerhead ribozyme on fas expression and Fas-mediated apoptosis of CTL cell line CTLL-2 cells, the cDNA of an anti-Fas hammerhead ribozyme was synthesized, its expression plasmid was constructed and transfected into CTLL-2 cells by electroporation. fas expression of CTLL-2 cells was detected by RT-PCR and Western blot. CTLL-2 cell viability was measured using MTT assay when co-cultured with mouse T cell leukemia cell line EL4 cells that highly expressed Fas ligand (FasL). Meanwhile, caspase-3 proteolytic activity was detected, and cell apoptosis was measured by flow cytometry and Hochest-PI double staining. Killing activity of CTLL-2 cells was detected by lactate dehydrogenase (LDH) releasing assay in vitro. Results showed that the expression of both Fas mRNA and protein in CTLL-2 cells were decreased after transfection of anti-Fas ribozyme. Compared with mock- transfected group and mutant ribozyme-transfected group, viability of CTLL-2 cells co-cultured with EL4 cells was increased significantly and cells killing activity was enhanced after transfected with anti-Fas ribozyme, while the caspase-3 activity and apoptosis rate was significantly decreased. The results demonstrated anti-Fas ribozyme could efficiently cleave Fas and inhibit Fas-mediated apoptosis of CTLL-2 cells to improve their viability. Our study made a basis for enhancing CTLL-2 cells anti-leukemia effect in DLI.

关键词： Hammerhead ribozyme anti-Fas CTLL-2 cells apoptosis tumor immunity

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D-CONVERGENCE AND STABILITY OF A CLASS OF LINEAR MULTISTEP METHODS FOR NONLINEAR DDES

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Journal of Computational Mathematics 2000年第2期18卷 199-206页

作者： Cheng-jian Zhang Xiao-xin Liao (department of Mathematics, huazhong university of science and technology, wuhan 430074, china) 华中科技大学湖北武汉 430074

Presents information on a study which dealt with the error behavior and the stability analysis of a class of linear multistep methods with the Lagrangian interpolation as applied to the nonlinear delay differential eq... 详细信息

关键词： D-convergence stability multistep methods nonlinear DDEs

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Combined splenocaval or mesocaval C shunt and portoazygous devascularization in the treatment of portal hypertension: analysis of 150 cases

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Hepatobiliary & Pancreatic Diseases International 2006年第1期5卷 70-73页

作者： Liu-Shun Feng and Xiao-Ping Chen Hepatic Surgery Center, tongji Hospital, tongji medical college, huazhong university of science and technology, wuhan 430030, china Hepatic Surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. Fengliushun@***

BACKGROUND: Portal hypertension is a common disease and its major surgical therapeutic approaches include devascularization and shunting. This study was undertaken to investigate the effects of combined splenocaval or mesocaval C shunt and portoazygous devascularization (combined procedures) on portal hypertension. METHODS: The clinical data of 150 patients with portal hypertension who had undergone combined procedures at the First Affiliated Hospital of Zhengzhou university from May1990 to May 2003 were analyzed retrospectively. RESULTS: The mean free portal pressure (FPP) was 25.6±1.83 mmHg, 18.0±2.07 mmHg and 18.4±2.19 mmHg before operation, after splenectomy plus splenocaval or mesocaval C shunt, and combined procedures, respectively. There was no operative death in all patients. The 1-7 year follow-up of 100 patients showed rebleeding in 3 patients, encephalopathy in 4, thrombosis of artificial vascular graft in 3, and dying from liver failure in 2. CONCLUSIONS: The combined procedures can not only decrease portal pressure but also preserve hepatic blood flow to some extent. It may be one of the best choices for treating portal hypertension in china.

关键词： hypertension portal vein blood vessel prosthesis devascularization shunting

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LAMINATED PIEZOELECTRIC PLATE FOR ACTIVE UNDERWATER ACOUSTIC CONTROL

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Acta Mechanica Solida Sinica 2001年第4期14卷 306-311页

作者： Zhao Hanzhong (department of Mechanics,huazhong university of science and technology,wuhan 430074,china) department of Mechanics Huazhong Univ. of Sci. and Technol. Wuhan 430074 China

The pressure reflected from a bi-laminated piezoelectric plate hasbeen determined using the Thomson-Haskell matrix method. The plate iscomposed of a piezoelectric layer with grounded vacuum and An elasticlayer in contact with the fluid. An incident plane wave in the fluidmedium strikes the plate at dif- Ferent angles. The required electricpotential across the piezoelectric layer to cancel the reflectionfrom the Fluid/elastic boundary has been determined for thepiezoelectric material PZT-5 at various thickness parame- Ters andincident frequencies.

关键词： underwater acoustic control laminated piezoelectric plate acousticscattering acoustic radiation

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ACOUSTIC REFLECTION FROM A BAFFLED ELASTIC/PIEZOELECTRIC PLATE

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Acta Mechanica Solida Sinica 2002年第2期15卷 156-162页

作者： Zhao Hanzhong (department of Mechanics,huazhong university of science and technology,wuhan 430074,china) department of Mechanics Huazhong Univ. of Sci. and Technol. Wuhan 430074 China

A finite difference/boundary integral procedure to determine theacoustic reflected pressure from a fluid-loaded bi-laminated plate isdescribed. The bi-laminate is composed of a piezoelectric layer andan elastic layer in contact with the fluid, and is held by anacoustically hard baffle. In the numerical model, the fluid pressureat fluid/solid interface is replaced by a continuum of point sourcesweighted by the normal acceleration of the elastic plate, and thegoverning equation system is solved in the solid domain.

关键词： acoustic reflection baffled palte finite difference boundary integral

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Exposure to persistent organic pollutants as potential risk factors for developing diabetes

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science china Chemistry 2010年第5期53卷 980-994页

作者： WANG ChunXiang,XU ShunQing,LV ZiQuan,LI YuanYuan,WANG YouJie & CHEN Tian Key Laboratory of Environment and Health,Ministry of Education School of Public Health,tongji medical college,huazhong university of science and technology,wuhan 430030,china 1. Key Laboratory of Environment and Health Ministry of Education School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Persistent organic pollutants(POPs),such as dioxins,polychlorinated biphenyls(PCBs) ,and organochlorine pesticides(OCPs),which are synthetic chemicals or by-products with an intrinsic resistance to natural degradation processes,are released into the environment,resulting in the widespread dispersal and accumulation in the environment,as well as in human and ecological food *** to their ubiquity in the environment and lipophilic properties,there is emerging concern over the potential risks of human exposure to *** growing evidence indicated that exposure to POPs might be strongly associated with increased risk of a worldwide epidemic of diabetes,especially type 2 diabetes,suggesting that POPs might play a key role in their *** on summary of the related studies,this paper reviews the epidemiologic and experimental data that addresses the association between increased risk of diabetes and POP exposure,including dioxins,PCBs,OCPs,polybrominated flame retardants(PBFRs),and some environmental *** potential mechanisms whereby POPs cause diabetes were discussed,such as alterations in lipid metabolism,in glucose transport,in insulin signaling pathway,in steroid metabolism,and disruption of endocrine system,induction of tumor necrosis factor-α(TNF-α) .However,with respect to diabetes,some of the evidence on POPs linked to risk of diabetes was suggestive of a direct or indirect association but was limited or *** research is urgently needed for determining the relative contribution of POPs to diabetes and elucidating the exact mechanisms.

关键词： persistent organic pollutants diabetes dioxins polychlorinated biphenyls organochlorine pesticides

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Detection of Fetal Nucleated Erythrocytes and Fetal DNA from Peripheral Blood of Pregnant Women

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Journal of huazhong university of science and technology(medical sciences) 2003年第1期23卷 65-67页

作者：张铭陈汉平 departmentofObstetricsandGynecology TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology

In order to search for a more reliable method of sorting fetal nucleated red blood cells (NRBCs) and DNA from maternal peripheral blood and to identify origin of NRBCs and DNA, NRBCs were isolated from peripheral blood of 88 pregnant women by density gradient centrifugation and fluorescence activated cell sorter (FACS) respectively. Nested polymerase chain reaction was used to detect normal male SRY gene from blood plasma DNA of 65 pregnant women. The results revealed that fetal NRBCs were found in 14 of 27 maternal samples by density gradient centrifugation. The number of cells was from 1 to 10. Using FACS, CD71 + cells were identified among all 61 samples. The frequency was (0.35±0.25)×10 -2; The detectable rate of the SRY gene of blood plasma DNA from 46 women carrying male fetuses was 65.22 % (30/46). Non-detectable rate for 19 women carrying female fetuses was 94.74 % (18/19). It was concluded that the methods of sorting fetal NRBCs and DNA have already made great progress. The methods for fetal NRBCs and plasma DNA from maternal peripheral blood to diagnose genetic diseases seem to be the best methods of noninvasive prenatal diagnosis.

关键词： nucleated red blood cells fetal DNA prenatal diagnosis

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LAMINATED VISCOELASTIC PLATE WITH INCLUSIONS FOR PASSIVE UNDER-WATER ACOUSTIC CONTROL

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Acta Mechanica Solida Sinica 2002年第1期15卷 23-29页

作者： Zhao Hanzhong (department of Mechanics,huazhong university of science and technology,wuhan 430074,china) department of Mechanics Huazhong Univ. of Sci. and Technol. Wuhan 430074 China

The effectiveness of a fluid-loaded sandwiched viscoelastic layerin suppressing acoustic scat- tering and radiation has been estimatedusing the Thomson-Haskell matrix method. The GU (Gaunaurd andUberall) resonance theory has been adopted to handle three types ofviscoelastic substrates with air -filled in- Clusions. The effects ofthe varying thickness of the substrates, the volume fractions of theinclusions and fre- Quencies of incident waves have been studied todetermine the optimal strategies of signal reduction. In theInvestigation, the monopole resonance phenomena of the inclusionshave been taken into account.

关键词： underwater acoustic control laminated viscoelastic plate air-filledinclusion

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