检索结果-南通市图书馆

Asian Herpetological Research 2015年第1期6卷 59-65页

作者： Yi GENG Xingxing LIU Yan ZHOU Kaiyu WANG Xi PENG Zhijun ZHONG Xiaoli HUANG defang chen College of Veterinary Medicine Sichuan Agricultural University Department of Aquaculture Sichuan Agricultural University

Loop-mediated isothermal ampliifcation （LAMP） is a novel nucleic acid diagnostic method that can amplify rapidly a target template under isothermal conditions. In this study, a LAMP assay for rapid detection of Chinese giant salamander ranavirus（CGSRV） was developed from culture isolates and clinical samples. The LAMP assay was developed by designing one set of four speciifc primers, targeting the major capsid protein （MCP） gene of CGSRV. Reaction time and temperature were optimal for 40 min at 62°C. The developed LAMP assay is speciifc and highly sensitive for CGSRV detection, the detection limit could reach about 5 copies of cloned viral genomic fragments. The sensitivity of the LAMP assay was about 1000 and 10-fold higher than that of both conventional and nested PCR, respectively. The LAMP ampliifcation produces a typical ladder-like pattern of products on an agarose gel that can be visually inspected after addition of ethidium bromide. The LAMP assay was evaluated further with clinical samples, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for the detection of CGSRV.

关键词： CGSRV Loop-mediated isothermal ampliifcation (LAMP) ranavirus Chinese giant salamander

Pathological Changes in Andrias davidianus Infected with Chinese Giant Salamander Ranavirus

在线全文

学校读者我要写书评

暂无评论

Asian Herpetological Research 2016年第4期7卷 258-264页

作者： Yi GENG Matthew James GRAY Kaiyu WANG defang chen Ping OUYANG Xiaoli HUANG Changliang HE Zhijun ZHONG Zexiao YANG College of Veterinary Medicine Sichuan Agricultural University Center for Wildlife Health University of Tennessee Department of Aquaculture Sichuan Agricultural University

Chinese giant salamander ranavirus （CGSRV） is an emerging pathogen in captive populations of the Chinese giant salamander （Andrias davidianus）. We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140-160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander.

关键词： pathology CGSRV Andrias davidianus ranavirus China

Multi-exit self-distillation with appropriate teachers

在线全文

学校读者我要写书评

暂无评论

Frontiers of Information Technology & Electronic Engineering 2024年第4期25卷 585-599页

作者： Wujie SUN defang chen Can WANG Deshi YE Yan FENG Chun chen College of Computer Science and Technology Zhejiang UniversityHangzhou 310000China

Multi-exit architecture allows early-stop inference to reduce computational cost,which can be used in resource-constrained *** works combine the multi-exit architecture with self-distillation to simultaneously achieve high efficiency and decent performance at different network ***,existing methods mainly transfer knowledge from deep exits or a single ensemble to guide all exits,without considering that inappropriate learning gaps between students and teachers may degrade the model performance,especially in shallow *** address this issue,we propose Multi-exit self-distillation with Appropriate TEachers(MATE)to provide diverse and appropriate teacher knowledge for each *** MATE,multiple ensemble teachers are obtained from all exits with different trainable *** exit subsequently receives knowledge from all teachers,while focusing mainly on its primary teacher to keep an appropriate gap for efficient knowledge *** this way,MATE achieves diversity in knowledge distillation while ensuring learning *** results on CIFAR-100,TinyImageNet,and three fine-grained datasets demonstrate that MATE consistently outperforms state-of-the-art multi-exit self-distillation methods with various network architectures.

关键词： Multi-exit architecture Knowledge distillation Learning gap

Dietary isoleucine improved flesh quality,muscle antioxidant capacity,and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2021年第4期12卷 1384-1398页

作者： Qin Jiang Mingyao Yan Ye Zhao Xiaoqiu Zhou Long Yin Lin Feng Yang Liu Weidan Jiang Pei Wu Yan Wang defang chen Shiyong Yang Xiaoli Huang Jun Jiang College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130China Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityYa’an 625014China

Background:Muscle is the complex and heterogeneous tissue,which comprises the primary edible part of the trunk of fish and *** studies have shown that dietary isoleucine(Ile)exerts beneficial effects on growth in aquatic ***,there were limited studies regarding the benefits of Ile on fish muscle and their effects on flesh quality and muscle ***,this study was conducted to explore whether dietary Ile had affected flesh quality and muscle growth in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂).Methods:A total of 630 hybrid fish,with an initial average body weight of 33.11±0.09 g,were randomly allotted into seven experimental groups with three replicates each,and respectively fed seven diets with 5.0,7.5,10.0,12.5,15.0,17.5,and 20.0 g Ile/kg diets for 8 ***:In the present study,we demonstrated that Ile significantly:(1)increased muscle protein and lipid contents and the frequency distribution of myofibers with≤20μm and≥50μm of diameter;(2)improved pH value,shear force,cathepsin B and L activities,hydroxyproline content,resilience,cohesiveness,and decreased cooking loss,lactate content,hardness,springiness,gumminess,and chewiness;(3)decreased reactive oxygen species(ROS),malondialdehyde(MDA),and protein carbonyl(PC)contents,GCLC and Keap1 mRNA levels,and up-regulated CuZnSOD,CAT,GPX1a,GST,and Nrf2 mRNA levels;(4)up-regulated the insulin-like growth factor 1,2(IGF-1,IGF-2),insulin-like growth factor 1 receptor(IGF-1R),proliferating cell nuclear antigen(PCNA),Myf5,Myod,Myog,Mrf4,and MyHC mRNA levels,and decreased MSTN mRNAlevel;(5)increased muscle protein deposition by activating AKT-TOR-S6K1 and AKT-FOXO3a signaling ***:These results revealed that dietary Ile improved flesh quality,which might be due to increasing nutritional content,physicochemical,texture parameters,and antioxidant ability;promoting muscle growth by affecting myocytes hyperplasia and hypertrophy,and muscle protein depos

关键词： Antioxidant capacity Flesh quality Hybrid bagrid catfish Isoleucine Myogenesis Protein deposition

在线全文

学校读者我要写书评

暂无评论

关于区域复合图书馆的研究

情报理论与实践 2007年第4期30卷 505-507页

作者：陈德芳南通大学图书馆江苏南通226007

本文通过对区域复合图书馆的研究,使人们认识到对区域复合图书馆的研究不仅有助于认识和把握图书馆的发展方向,达到扩大资源系统收集,确保专藏、复合资源合理建设,节省经费的总目标,更有利于区域创新体系的发展和提升,有利于地域文化的... 详细信息

本文通过对区域复合图书馆的研究,使人们认识到对区域复合图书馆的研究不仅有助于认识和把握图书馆的发展方向,达到扩大资源系统收集,确保专藏、复合资源合理建设,节省经费的总目标,更有利于区域创新体系的发展和提升,有利于地域文化的进步和发展,有利于为区域经济的发展提供保障等。

关键词：复合图书馆信息资源发展研究

关于区域复合图书馆的研究续论——数字信息的永久保存

在线全文

学校读者我要写书评

暂无评论

图书馆工作与研究 2008年第12期 8-10页

作者：陈德芳南通大学图书馆

本文基于区域复合图书馆的研究,阐述了数字信息永久保存的必要性、紧迫性和可能性。提出了区域复合图书馆有利于数字信息永久保存的三个方面:区域复合图书馆的共建与自建相结合的结构模式;国内外技术参照;区域复合图书馆适合发展成为国... 详细信息

本文基于区域复合图书馆的研究,阐述了数字信息永久保存的必要性、紧迫性和可能性。提出了区域复合图书馆有利于数字信息永久保存的三个方面:区域复合图书馆的共建与自建相结合的结构模式;国内外技术参照;区域复合图书馆适合发展成为国家数字信息保存的分基地。

关键词：复合图书馆区域数字信息永久保存

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

以高校教学联合体为平台的学科化服务

图书馆学研究 2010年第8期 70-72页

作者：陈德芳南通大学图书馆

以高校教学联合体为平台的学科化服务,即以高校教学联合体为服务平台,以联合体的专用网站为服务入口,以联合体内的相同学科为服务单元,集联合体内不同学校相同学科信息资源和人力资源,基于多元化的工作内容,实行纵向的服务体系,采用网... 详细信息

以高校教学联合体为平台的学科化服务,即以高校教学联合体为服务平台,以联合体的专用网站为服务入口,以联合体内的相同学科为服务单元,集联合体内不同学校相同学科信息资源和人力资源,基于多元化的工作内容,实行纵向的服务体系,采用网络化服务与人工服务相结合、分工与合作相结合等服务手段,开展学科化服务。该服务不仅能推动高校教学联合体更加有效的实施,而且能提升图书馆自身服务的能力和品牌特色,促进图书馆事业的可持续发展。

关键词：教学联合体学科化服务资源共享

在线全文

学校读者我要写书评

暂无评论

基于多项式正态变换的年径流频率计算研究

水力发电学报 2019年第11期38卷 58-69页

作者：陈得方宋松柏西北农林科技大学水利与建筑工程学院西北农林科技大学旱区农业水土工程教育部重点实验室

水文设计值精度直接影响到工程规模的确定。应用多项式正态变换(PNT)法推求给定频率的设计值(分位数),提高水文频率计算精度。采用蒙特卡洛法进行模拟试验,对比分析三阶多项式系数的矩(PM)法、L-阶线性矩(LM)法、最小二乘(LS)法和Fisher... 详细信息

水文设计值精度直接影响到工程规模的确定。应用多项式正态变换(PNT)法推求给定频率的设计值(分位数),提高水文频率计算精度。采用蒙特卡洛法进行模拟试验,对比分析三阶多项式系数的矩(PM)法、L-阶线性矩(LM)法、最小二乘(LS)法和Fisher-Cornish(FC)不对称展开法的参数求解方法,并选用黄河流域五个测站天然年径流资料,开展三阶多项式正态变换法的年径流频率计算研究。结果表明:4种方法求解所得的多项式系数均能推求给定频率下的年径流设计值,且设计值序列整体与实测序列拟合效果良好,对数据的正态性转换和拟合效果排序结果为最小二乘法、L-阶线性矩法、Fisher-Cornish不对称展开法、矩法。

关键词：多项式正态变换法水文频率蒙特卡洛法黄河流域拟合效果

水环境中Ni^2+对鲤鱼鳃和肝脏的组织损伤

在线全文

学校读者我要写书评

暂无评论

西北农业学报 2020年第2期29卷 175-181页

作者：陈代宇杨飞陈德芳耿毅四川农业大学动物科技学院四川温江611130 四川农业大学动物医学院四川温江611130

为研究水环境中Ni2+对鲤鱼鳃和肝脏的组织损伤,将鲤鱼(Cyprinus carpio)暴露在Ni2+浓度为0、0.55、0.62、0.72、0.83、0.96、1.09、1.25 mmol/L的水环境中进行急性毒性试验,并通过组织切片观察鳃和肝脏的组织病理变化。结果显示,Ni2+对... 详细信息

为研究水环境中Ni2+对鲤鱼鳃和肝脏的组织损伤,将鲤鱼(Cyprinus carpio)暴露在Ni2+浓度为0、0.55、0.62、0.72、0.83、0.96、1.09、1.25 mmol/L的水环境中进行急性毒性试验,并通过组织切片观察鳃和肝脏的组织病理变化。结果显示,Ni2+对鲤鱼的96 h半数致死浓度(LC50)为0.72 mmol/L,安全浓度(SC)为0.0072 mmol/L。高浓度Ni2+(1.25 mmol/L)试验组鲤鱼的鳃小片呼吸上皮细胞坏死、脱落,肝血窦淤血,肝细胞核固缩;中低浓度Ni2+(0.72~1.09 mmol/L)试验组鲤鱼的鳃小片呼吸上皮水肿浮离、细胞增生,血窦充血,肝细胞胞浆空泡化。表明水环境中的Ni2+浓度高于0.0072 mmol/L时会对鲤鱼的鳃和肝脏造成急性毒性,导致鳃和肝脏出现明显的组织病理变化。

关键词：鲤鱼重金属镍急性毒性病理

在线全文

学校读者我要写书评

暂无评论

我馆回溯建库业务外包的实践和体会

图书馆建设 2001年第4期 37-39页

作者：缪融陈德芳南通师范学院图书馆 226007

本文回顾总结了本馆在回溯建库工作中的经验和教训，提出了中小型馆利用业务外包进行回溯建库的可行性．

关键词：图书馆自动化建设数据库建设回溯建库业务外包数据质量控制建库方法扫描工作