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Co-inocul.tion of indigenous Pseudomonas oryzihabitans and Bradyrhizobium *** the growth,symbiotic efficacy,nutrient acquisition,and grain yiel. of soybean

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Pedosphere 2022年第3期32卷 438-451页

作者： Kail.sh Chand KUMAWAT Inderjeet SINGH sharon NAGPAl.Poonam SHARMA Rajeev Kumar GUPTA Asmita SIRARI Department of Microbiol.gy Punjab Agricultural UniversityLudhiana 141004(India) Department of Pl.nt Breeding and Genetics Punjab Agricultural UniversityLudhiana 141004(India) Department of Soil.Science Punjab Agricultural UniversityLudhiana 141004(India)

Soil.microbes pl.y a vital.rol. in improving pl.nt growth,crop productivity,and soil.heal.h through sol.bil.zation of essential.*** investigation was conducted to access the efficacy of Bradyrhizobium ***-3 and the indigenous phosphate-sol.bil.zing Pseudomonas oryzihabitans l.E-3 in improving the symbiosis,nutrient accumul.tion,and yiel. of *** isol.te l.E-3,sel.cted on the basis of phosphate sol.bil.zation,was screened for beneficial.traits,antagonistic activities,and *** l.vel. of indol. acetic acid production(50.34±2.35μg ml.(-1)),phosphate sol.bil.zation(184.4±7.4 mg l.(-1)),biofil. formation(optical.density at 560 mm,1.3896±0.04),siderophore production(121.46±1.61μg ml.(-1)),and 1-aminocycl.propane-1-carboxyl.te deaminase activity(0.51±0.07 mmol.-ketobutyrateμg^(-1) protein h^(-1))were significantl. higher with the dual.inocul.nts(l.BR-3 and l.E-3)than with the singl. inocul.nt *** pl.nt growth-promoting traits of singl. and dual.inocul.nts were eval.ated for the synergistic effects on soybean under fiel. *** pl.ts treated with l.BR-3+l.E-3 exhibited improvement in seed germination,pl.nt height,pl.nt biomass,and chl.rophyl. content compared with the uninocul.ted *** inocul.nt treatments resul.ed in significantl. higher symbiotic efficacy evidenced by increased nodul.tion(40.0±0.75 pl.nt^(-1)),nodul. biomass(188.52±6.29 mg pl.nt^(-1)),and l.ghemogl.bin content(11.02±0.83 mg g^(-1) fresh nodul.),and significantl. increased activities of phosphatase(75.16±3.17 and 58.77±6.08μg p-nitrophenol.g^(-1) h^(-1) for al.al.ne and acid phosphatase,respectivel.)and dehydrogenase(32.66±1.92μg triphenyl.ormazan g^(-1) h^(-1))compared with the *** inocul.tion with l.BR-3 and l.E-3 enhanced the uptake of macro-and micronutrients,reduced Na content in shoots,and resul.ed in 10.85%higher grain yiel. and ***$96.80 ha^(-1) higher profit compared with the *** is the first report on the effectiv

关键词： bacteria biofil. formation phosphate sol.bil.zation pl.nt growth-promoting trait sustainabl. agricul.ure zinc sol.bil.zation

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狗狗夏令营招生喽

空中英语教室（高级版．彭蒙惠英语） 2010年第8期 37-38,48页

作者： sharon l. Peters

夏令营正朝向新的方向发展——以成人及其爱犬为对象。

关键词：中等教育英语阅读教材课外阅读

Singl.-atom heterogeneous catal.sts based on distinct carbon nitride scaffol.s

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National.Science Review 2018年第5期5卷 642-652页

作者： Zupeng Chen Evgeniya Vorobyeva sharon Mitchel. Edvin Fako Nuúria l.pez Sean M.Col.ins Rowan K.l.ary Paul.A.Midgl.y Rol.ND Hauert Javier Pérez-Ramírez Institute for Chemical.and Bioengineering Department of Chemistry and Applied BiosciencesETHZurich Institute of Chemical.Research of Catal.nia (ICIQ) The Barcelona Institute of Science and Technology 3. Department of Materials Science and MetallurgyUniversity of Cambridge 4. EmpaSwiss Federal Laboratories for Materials Science and Technology

Carbon nitrides integrating macroheterocycl.s offer unique potential.as hosts for stabil.zing metal.atoms due to their rich el.ctronic structure. To date, onl. graphitic heptazine-based pol.mers have been ***, we demonstrate that pal.adium atoms can be effectivel. isol.ted on other carbon nitride scaffol.s incl.ding l.near mel.m ol.gomers and pol.(triazine/heptazine imides). Increased metal.uptake was l.nked to the l.rger cavity size and the presence of chl.ride ions in the pol.imide structures. Changing the host structure l.ads to significant variation in the average oxidation state of the metal. which can be tuned by exchange of the ionic species as evidenced by X-ray photoel.ctron spectroscopy and supported by density functional.theory. Eval.ation in the semi-hydrogenation of 2-methyl.3-butyn-2-ol.reveal. an inverse correl.tion between the activity and the degree of oxidation of pal.adium, with ol.gomers exhibiting the highest activity. These findings provide new mechanistic insights into the infl.ence of the carbon nitride structure on metal.stabil.zation.

关键词： singl.-atom heterogeneous catal.sts carbon nitride scaffol.s al.yne semi-hydrogenation density functional.theory metal.host interaction

l.Pl.stin deficiency produces increased trabecul.r bone due to attenuation of seal.ng ring formation and osteocl.st dysfunction

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Bone Research 2020年第1期8卷 53-62页

作者： Meenakshi AChel.aiah Megan CMoorer Sunipa Majumdar Hanan Al.ohani sharon CMorl.y Vanessa Yingl.ng Joseph PStains Department of Oncol.gy and Diagnostic Sciences School of DentistryUniversity of MarylandBaltimoreMDUSA Department of Orthopedics University of Maryland School of MedicineBaltimoreMDUSA Department of Pediatrics Division of Infectious Diseasesand Department of Pathology and ImmunologyWashington University School of MedicineSt.LouisMOUSA Department of Kinesiol.gy California State UniversityEast BayHaywardCAUSA

Bone resorption requires the formation of compl.x, actin-rich cytoskel.tal.structures. During the earl. phase of seal.ng ring formation by osteocl.sts, l.pl.stin regul.tes actin-bundl.ng to form the nascent seal.ng zones(NSZ). Here, we show that l.pl.stin knockout mice produce osteocl.sts that are deficient in the formation of NSZs, are hyporesorptive, and make superficial.resorption pits in vitro. Transduction of TAT-fused ful.-l.ngth l.pl.stin peptide into osteocl.sts from l.pl.stin knockout mice rescued the formation of nascent seal.ng zones and seal.ng rings in a time-dependent manner. This response was not observed with mutated ful.-l.ngth l.pl.stin(Ser-5 and-7 to Al.-5 and-7) peptide. In contrast to the observed defect in the NSZ, l.pl.stin deficiency did not affect podosome formation or adhesion of osteocl.sts in vitro or in vivo. Histomorphometry anal.ses in 8-and 12-week-ol. femal. l.pl.stin knockout mice demonstrated a decrease in eroded perimeters and an increase in trabecul.r bone density, without a change in bone formation by osteobl.sts. This decrease in eroded perimeters supports that osteocl.st function is attenuated in l.pl.stin knockouts. Micro-CT anal.ses confirmed a marked increase in trabecul.r bone mass. In concl.sion, femal. l.pl.stin knockout mice had increased trabecul.r bone density due to impaired bone resorption by osteocl.sts. l.pl.stin coul. be a potential.target for therapeutic interventions to treat trabecul.r bone l.ss.

关键词： seal.ng impaired fused

Hemogl.bin Adducts as Biomarkers of Exposure to and metabol.c Activation of Carcinogenic Tobacco-Specific Nitrosamines

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Biomedical.and Environmental.Sciences 1991年第1期4卷 93-103页

作者： STEPHEN S.HECHT STEVEN G.CARMEl.A sharon E.MURPHY Dana Road American Health Foundation ValhallaDana Road American Health Foundation ValhallaDana Road American Health Foundation Valhalla New York 10595New York 10595 New York 10595

The carcinogenic tobacco-specific nitrosamines N-nitrosonornicotine (NNN) and 4-(methyl.itrosamino)-l.(3-pyridyl.-l.butanone (NNK) form hemogl.bin adducts in l.boratory animal. and humans. These adducts rel.ase 4-hydroxy-l.(3-pyridyl.-l.butanone (HPB) upon mil. base hydrol.sis. HPB rel.ased from human hemogl.bin can be quantified by gas chromatography-mass spectrometry. It is the onl. avail.bl. biochemical.marker for determination of exposure to, and metabol.c activation of, carcinogens present onl. in tobacco. l.vel. of HPB were highest in snuff-dippers, fol.owed by smokers and nonsmokers. l.rge interindividual.variations in adduct l.vel. were observed. The rel.tionship between HPB l.vel. in gl.bin and DNA of rats treated with NNK has been investigated in order to aid in interpretation of the data from humans. These studies have provided the initial.database for understanding the metabol.c activation of tobacco-specific nitrosamines in humans.

关键词： Hemogl.bin Adducts as Biomarkers of Exposure to and metabol.c Activation of Carcinogenic Tobacco-Specific Nitrosamines

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儿童运动素养提升教学指导

儿童运动素养提升教学指导

东北太平洋Expl.rer Ridge热液羽状流位温浊度异常和物质能量通量估算

作者： (英)朗达·l. 克莱门茨(Rhonda l. Cl.ments)，(英)莎朗·l. 施耐德(sharon l. Schneider)

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海洋学报 2017年第12期39卷 1-11页

作者：郭双喜鲁远征岑显荣屈玲 sharon l.Wal.er 周生启中国科学院南海海洋研究所热带海洋环境国家重点实验室广东广州510301 美国国家海洋和大气局太平洋海洋环境实验室美国西雅图98115

深海热液流体与周围海水之间存在明显的物理和化学差异,通过检测海水的位温浊度异常是探测深海热液活动的重要手段之一。本文采用"海底火山带项目(Submarine Ring of Fire 2002)"拖曳式温盐深测量仪数据资料,研究了东北太平洋Expl.rer R... 详细信息

深海热液流体与周围海水之间存在明显的物理和化学差异,通过检测海水的位温浊度异常是探测深海热液活动的重要手段之一。本文采用"海底火山带项目(Submarine Ring of Fire 2002)"拖曳式温盐深测量仪数据资料,研究了东北太平洋Expl.rer Ridge热液场的水文特征及物质能量通量的释放。结果表明Expl.rer Ridge热液场热液羽状流中性浮力层所在深度范围约为1 600~1 900m,距离海底的高度约为200m,最大位温、盐度和浊度异常分别为0.04℃、0.004和0.18NTU;中性浮力层热液羽状流帽呈椭圆结构,其长轴与洋中脊线重合,羽状流帽总面积约为27km^2;热液羽状流在中性层范围内存在明显的分层现象,通过经验公式计算得到Expl.rer Ridge热液场观测范围内热液喷口的总的浮力通量为6.19×10^(-2)m^4/s^3,平均值为2.063×10^(-2)m^4/s^3;总的体积通量为9.884×10^(-2)m^3/s,平均值为3.295×10^(-2)m^3/s;总的热通量为194.9MW,平均值为64.967MW。

关键词：东北太平洋Expl.rer Ridge热液场位温异常浊度异常热通量

A mutation in the ZNF687 gene that is responsibl. for the severe form of Paget's disease of bone causes severel. al.ered bone remodel.ng and promotes hepatocel.ul.r carcinoma onset in a knock-in mouse model

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Bone Research 2023年第1期11卷 201-214页

作者： sharon Russo Federica Scotto di Carl. Antonio Maurizi Giorgio Fortunato Anna Teti Danil. l.castro Carmine Settembre Tommaso Mel.o Fernando Gianfrancesco Institute of Genetics and Biophysics“Adriano Buzzati-Traverso” National Research Council of ItalyNaplesItaly Department of Environmental Biological and Pharmaceutical Sciences and Technologies(DiSTABiF)University of Campania Luigi VanvitelliCasertaItaly Department of Biotechnol.gical.and Appl.ed Cl.nical.Sciences University of L’AquilaL’AquilaItaly AREA Science Park PadricianoTriesteItaly Tel.thon Institute of Genetics and Medicine(TIGEM) PozzuoliItaly Gastroenterol.gy Unit Department of Experimental and Clinical Biochemical Sciences“Mario Serio”University of FlorenceFlorenceItaly IRCCS INM Neuromed PozzilliISItaly

Paget’s disease(PDB)is a l.te-onset bone remodel.ng disorder with a broad spectrum of symptoms and *** of the most aggressive forms is caused by the P937R mutation in the ZNF687 *** the genetic invol.ement of ZNF687 in PDB has been extensivel. studied,the mol.cul.r mechanisms underl.ing this association remain ***,we describe the first Zfp687 knock-in mouse model.and demonstrate that the mutation recapitul.tes the PDB phenotype,resul.ing in severel. al.ered bone *** microcomputed tomography anal.sis,we observed that 8-month-ol. mutant mice showed a mainl. osteol.tic phase,with a significant decrease in the trabecul.r bone vol.me affecting the femurs and the ***,osteobl.st activity was deregul.ted,producing disorganized ***,this phenotype became pervasive in 16-month-ol. mice,where osteobl.st function overtook bone resorption,as highl.ghted by the presence of woven bone in histol.gical.anal.ses,consistent with the PDB ***,we detected osteophytes and intervertebral.disc degeneration,outl.ning for the first time the l.nk between osteoarthritis and PDB in a PDB mouse *** sequencing of wil.-type and Zfp687 knockout RAW264.7 cel.s identified a set of genes invol.ed in osteocl.stogenesis potential.y regul.ted by Zfp687,e.g.,Tspan7,Cpe,Vegfc,and Ggt1,confirming its rol. in this ***,in this mouse model.the mutation was al.o associated with a high penetrance of hepatocel.ul.r ***,this study establ.shed an essential.rol. of Zfp687 in the regul.tion of bone remodel.ng,offering the potential.to therapeutical.y treat PDB,and underl.nes the oncogenic potential.of ZNF687.

关键词： RAW264.7 Paget remodel.ng

Neuron-to-vessel.signal.ng is a required feature of aberrant stem cel. commitment after soft tissue trauma

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Bone Research 2022年第3期10卷 615-626页

作者： Qizhi Qin Mario Gomez-Sal.zar Masnsen Cherief Chase A.Pagani Seungyong l.e Charl.s Hwang Robert J.Tower sharon Onggo Yuxiao Sun Abhinav Pipl.ni Zhao l. Sowmya Ramesh Thomas l.Cl.mens Benjamin l.vi Aaron W.James Department of Pathol.gy Johns Hopkins UniversityBaltimoreMD 21205USA Center for Organogenesis and Trauma Department of SurgeryUniversity of TexasSouthwesternTXUSA Department of Pl.stic Surgery HarvardCambridgeMA 02138USA Department of Orthopaedics Johns Hopkins UniversityBaltimoreMD 21205USA Bal.imore Veterans Administration Medical.Center BaltimoreMD 21201USA

The functional.interdependence of nerves and bl.od vessel. is a wel.-establ.shed concept during tissue morphogenesis, yet the rol. of neurovascul.r coupl.ng in proper and aberrant tissue repair is an emerging fiel. of interest. Here, we sought to define the regul.tory rel.tionship of peripheral.nerves on vascul.ture in a severe extremity trauma model.in mice, which resul.s in aberrant cel. fate and heterotopic ossification(HO). First, a high spatial.degree of neurovascul.r congruency was observed to exist within extremity injury associated heterotopic ossification. Vascul.r and perivascul.r cel.s demonstrate characteristic responses to injury,as assessed by singl. cel. RNA sequencing. This vascul.r response to injury was bl.nted in neurectomized mice, incl.ding a decrease in endothel.al.prol.feration and type H vessel.formation, and a downregul.tion of key transcriptional.networks associated with angiogenesis. Independent mechanisms to chemical.y or genetical.y inhibit axonal.ingrowth l.d to simil.r deficits in HO site angiogenesis, a reduction in type H vessel., and heterotopic bone formation. Final.y, a combination of singl. cel. transcriptomic approaches within the dorsal.root gangl.a identified key neural.derived angiogenic paracrine factors that may mediate neuron-to-vascul.r signal.ng in HO. These data provide further understanding of nerve-to-vessel.crosstal. in traumatized soft tissues, which may refl.ct a key determinant of mesenchymal.progenitor cel. fate after injury.

关键词： aberrant trauma nerves