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development and characterization of a microfluidic glucose sensing system based on an enzymatic microreactor and chemiluminescence detection

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Science China Chemistry 2012年第4期55卷 515-523页

作者： mOON B.-U. de vries m.g. WESTERINK B.H.C. VERPOORTE E. Biomonitoring and Sensoring Department of Pharmacy University of Groningen Antonius Deusinglaan 1 9713AV Groningen The Netherlands Pharmaceutical Analysis Department of Pharmacy University of Groningen Antonius Deusinglaan 1 9713AV Groningen The Netherlands Brains-on-Line PO Box 4030 9713AV Groningen The Netherlands

Chemiluminescence detection was developed as an alternative to amperometric detection for glucose analysis in a portable, microfluidicsbased continuous glucose monitoring system. Amperometric detection allows easy determination of hydrogen peroxide, a product of the glucose oxidasecatalyzed reaction of glucose with oxygen, by oxidation at a microelectrode. However, （micro）electrodes in direct contact with physiological sample are subject to electrode fouling, which leads to signal drift, decreased reproducibility and shortened detector lifetimes. moreover, there are a few species present in the body （e.g. ascorbic acid, uric acid） which can undergo oxidation at the same applied potential as hydrogen peroxide. These species can thus inter- fere with the glucose measurement, reducing detection specificity. The rationale for exploring chemiluminescence as opposed to amperometric detection is thus to attempt to improve the lifetime and reproducibility of glucose analysis for monitoring purposes, while reducing interference caused by other chemicals in the body. The study reported here represents a first step in this direction, namely the realization of a microfluidic device with integrated silicon photodiode for chemiluminescence detection of glucose. This microflow device uses a chaotic mixing approach to perform enzymatic conversion of glucose, followed by reaction of the hydrogen peroxide produced with luminol to produce light at 425 nm. The chemil reaction is catalyzed by horseradish peroxidase in the presence of iodophenol. The performance of the fabricated chip was characterized to establish optimal reaction conditions with respect to sample and reagent flow rates, pH, and concentrations. A linear calibra- tion curve was obtained for current response as a function of glucose concentration in the clinically relevant range between 2 and 10 mm, with a sensitivity of 39 pA/mm （R = 0.9963, one device, n = 3） and a limit of detection of 230 ktm （S/N - 3）.

关键词： microfluidic chip – glucose sensing system – enzymatic microreactor – chemiluminescence – chaotic mixing

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Fecal microbiota transplantation as novel therapy in gastroenterology:A systematic review

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World Journal of gastroenterology 2015年第17期21卷 5359-5371页

作者： Noortje g Rossen John K mac Donald Elisabeth m de vries geert R D'Haens Willem m de Vos Erwin g Zoetendal Cyriel Y Ponsioen department of gastroenterology and Hepatology Academic Medical Center Amsterdam1105 AZ AmsterdamThe Netherlands Division of gastroenterology Robarts Clinical trialsRobarts Research Institutethe University of Western OntarioLondonCanada and Amsterdamthe NetherlandsOntario N6A 5K8Canada departments of Bacteriology and Immunology and Veterinary Biosciences University of HelsinkiFI-00014 HelsinkiFinland Laboratory of microbiology Wageningen University6703 HA Wageningenthe Netherlands

AIm:To study the clinical efficacy and safety of Fecal microbiota transplantation(FmT).We systematically reviewed FmT used as clinical ***:We searched mEDLINE,EmBASE,the Cochrane Library and Conference proceedings from inception to July,*** effect of FmT was calculated as the percentage of patients who achieved clinical improvement per patient category,on an intention-to-treat ***:We included 45 studies;34 on Clostridium difficile-infection(CDI),7 on inflammatory bowel disease,1 on metabolic syndrome,1 on constipation,1 on pouchitis and 1 on irritable bowel syndrome(IBS).In CDI 90% resolution of diarrhea in 33 case series(n = 867) was reported,and 94% resolution of diarrhea after repeated FmT in a randomized controlled trial(RCT)(n = 16).In ulcerative colitis(UC) remission rates of 0% to 68% were found(n = 106).In Crohn's disease(CD)(n = 6),no benefit was *** IBS,70% improvement of symptoms was found(n = 13).100% Reversal of symptoms was observed in constipation(n = 3).In pouchitis,none of the patients(n = 8) achieved *** RCT showed significant improvement of insulin sensitivity in metabolic syndrome(n = 10).Serious adverse events were ***:FmT is highly effective in CDI,and holds promise in *** for CD,chronic constipation,pouchitis and IBS data are too limited to draw *** increases insulin sensitivity in metabolic syndrome.

关键词： Fecal microbiota transplantation microbiota Clostridium difficile infection Inflammatory boweldisease metabolic syndrome

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Novel Phialophora species from leaf-cutting ants (tribe Attini)

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Fungal Diversity 2014年第2期65卷 65-75页

作者： D.Attili-Angelis A.P.m.Duarte F.C.Pagnocca N.S.Nagamoto m.de vries J.B.Stielow g.S.de Hoog Center for the Study of Social Insects UNESP-São Paulo State UniversitySão PauloSPBrazil Division of microbial Resources CPQBAUniversity of CampinasCampinasSPBrazil department of Plant Protection UNESP-São Paulo State UniversityBotucatuSPBrazil Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre UtrechtThe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Sun Yat-sen memorial Hospital Sun Yat-sen UniversityGuangzhouChina Research Center for medical mycology Peking University Health Science CenterBeijingChina Shanghai Institute of medical mycology Changzheng HospitalSecond Military Medical UniversityShanghaiChina Basic Pathology department Federal University of ParanáStateCuritibaParanáBrazil King Abdulaziz University JeddahSaudi Arabia

Ants in the tribe Attini(Hymenoptera,Formicidae)maintain a 50 million-year-old lifestyle of co-evolution with symbiotic basidiomycetous fungi which they cultivate as essential source of ***,other microorganisms have been reported from ant habitats indicating a higher diversity of consistently associated species than established to ***,black yeast-like fungi have been reported as a possible symbiont,like a competitor of the actinomycete bacteria that grow in the integument of the *** the mating season,gynes of Atta capiguara and *** were collected from nests located in Botucatu,SP,Brazil and sampled using flotation *** analysis based on ITS,partial 28S(LSU)andβ-tubulin sequences revealed the occurrence of two novel species of Phialophora among the melanized fungi isolated.

关键词： Black yeasts Chaetothyriales gynes Phialophora Cyphellophora Atta Ant cuticle Neotropical fungal ecology

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Contemporary diagnostics and treatment options for female stress urinary incontinence

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Asian Journal of Urology 2018年第3期5卷 141-148页

作者： Allert m.de vries John P.F.A.Heesakkers department of Urology Radboud University Medical CenterNijmegenThe Netherlands

Stress urinary incontinence is not a deadly disease,but for the large population of women suffering from it,it is a very important *** in the continuously aging population all over the world,there is more and more need for treatment of this serious medical *** of female stress urinary incontinence exists already for *** the 20th century invasive treatments like Burch colposuspension and pubovaginal slings were the mainstay of surgical *** introduction of the midurethral sling made the procedure less invasive and accessible for more *** there are many options available and the field is developing *** recent years many new medical devices have been developed,that increase the number of treatment options available and make it possible to find a suitable solution for the individual patient based on subjective and objective results and the chances of *** manuscript provides an introduction to the therapeutical options that are available nowadays for female stress urinary incontinence.

关键词： Female Stress urinary incontinence Treatment Urinary incontinence Pelvic floor muscle training Suburethral slings

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Phylogeny and taxonomy of meristematic rock-inhabiting black fungi in the Dothideomycetes based on multi-locus phylogenies

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Fungal Diversity 2014年第2期65卷 127-165页

作者： E.Egidi g.S.de Hoog D.Isola S.Onofri W.Quaedvlieg m.de vries g.J.m.Verkley J.B.Stielow L.Zucconi L.Selbmann Dipartimento di Scienze Ecologiche e Biologiche(deB) Universitàdegli Studi della TusciaLargo dell’Universitàsnc01100 ViterboItaly Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre UtrechtThe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Sun Yat-sen memorial Hospital Sun Yat-sen UniversityGuangzhouChina Research Center for medical mycology Peking University Health Science CenterBeijingChina Shanghai Institute of medical mycology Changzheng HospitalSecond Military Medical UniversityShanghaiChina Basic Pathology department Federal University of ParanáStateCuritibaParanáBrazil King Abdulaziz University JeddahSaudi Arabia

The last decade has revealed an unexpected fungal diversity associated with natural rocks,often collected in environments influenced by harsh climatic *** the phylogenetic affiliations and the taxonomy of many of these extreme fungi,mainly within Dothideomycetes,the largest class of Ascomycota,have only partially been *** the present study we confirm that most rock inhabiting-fungi(RIF)are highly polyphyletic among Dothideomycetidae,mainly within the order Capnodiales,an order otherwise incorporating several families of major plant pathological *** taxa were identified within the two major and distinct clades of Teratosphaeriaceae,both comprising meristematic black *** one novel species and 13 new genera are proposed,based on ITS and partial nucLSU,RPB2 and BT2 sequences.

关键词： Black yeasts Capnodiales meristematic fungi microcolonial fungi Phylogeny Taxonomy

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modelling impacts of acid deposition and groundwater level on habitat quality and plant species diversity

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Ecological Processes 2016年第1期5卷 273-291页

作者： J.Kros J.P.mol-Dijkstra g.W.W.Wamelink g.J.Reinds Avan Hinsberg W.de vries Wageningen Environmental Research Wageningen University and ResearchP.O.Box 476700 AA WageningenThe Netherlands Netherlands Environmental Assessment Agency PO Box 3033720 BABilthovenWageningenThe Netherlands Environmental Systems Analysis group Wageningen University and ResearchP.O.Box 476700 AA WageningenThe Netherlands

Introduction:We quantified the effects of the site factors pH and nitrate(NO_(3))concentration in soil solution and groundwater level on the vegetation of terrestrial ecosystems for the Netherlands in response to changes in atmospheric nitrogen(N)and sulphur(S)deposition and groundwater level over the period *** assessment was made with the SmART2 model,a simple one-layer model including geochemical buffer processes,element cycling by litterfall,mineralisation and uptake,nitrogen transformation processes and element input through deposition,weathering and upward ***:To assess the effects of changes in abiotic site factors on the vegetation,we developed a simple plant diversity indicator for grassland,heathland and forest,based on the occurrence of target plant species and competing *** occurrence was calculated from the preferred ranges of each species for the NO_(3) concentration and pH in soil solution and mean spring groundwater *** in the plant diversity indicator were assessed from effects of changes in the occurrence of target and competing plant species in response to changes in mean spring groundwater level and in pH and NO_(3) concentration,as calculated with *** were made for combinations of five vegetation structure types(three forest types,semi-natural grassland and heathland)and seven soil types(three sandy soils,two clay soils,peat and loess soils)using a 250×250 m *** used data for atmospheric deposition and groundwater level in the past to assess trends between 1990 and 2010 and evaluated two future scenarios for the period 2010-2030:a Business as Usual and an Improved Environment ***:Comparison of model predictions on pH and NO_(3) with measured soil solution concentrations for forest showed a reasonable to good agreement for pH but rather poor for NO_(3).The largest impacts were found for the combination of the two Improved Environment ***:Reductions in N

关键词： Nutrient cycling Soil modelling Biodiversity Acidification Scenario analysis

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Long-term efficacy of a computer-tailored physical activity intervention for prostate and colorectal cancer patients and survivors:A randomized controlled trial

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Journal of Sport and Health Science 2023年第6期12卷 690-704,F0003页

作者： Rianne Henrica Johanna golsteijn Catherine Bolman denise Astrid Peels Esmee Volders Hein de vries Lilian Lechner department of Psychology Open University of the NetherlandsHeerlen6401 DLthe Netherlands department of Health Promotion Maastricht UniversityMaastricht6200 MDthe Netherlands

Background Physical activity(PA)can improve the physical and psychological health of prostate and colorectal cancer survivors,but PA behavior change maintenance is necessary for long-term health *** is a print-and web-based intervention in which prostate and colorectal cancer patients and survivors receive automatically generated,personalized feedback aimed at integrating PA into daily life to increase and maintain *** evaluated the long-term outcomes of OncoActive by examining the 12-month follow-up differences between OncoActive and a control group,and we explored whether PA was maintained during a 6-month non-intervention follow-up *** Prostate or colorectal cancer patients were randomly assigned to an OncoActive(n=249)or a usual care waitlist control group(n=229).OncoActive participants received PA advice and a *** outcomes(i.e.,Actigraph and self-report moderate-to-vigorous intensity PA(mVPA)min/week and days with≥30 min PA)and health-related outcomes(i.e.,fatigue,depression,physical functioning)were assessed at baseline,6 months,and 12 *** between groups and changes over time were assessed with multilevel linear regressions for the primary outcome(Actigraph mVPA min/week)and all additional *** At 12 months,OncoActive participants did not perform better than control group participants at Actigraph mVPA min/week,self-report mVPA min/week,or Actigraph days with *** self-report days with PA were significantly higher in OncoActive compared to the control *** health-related outcomes only long-term fatigue was significantly lower in *** exploratively examining PA within OncoActive,the previously found PA effects at the end of the intervention(6 months follow-up)were maintained at 12 ***,all PA outcomes improved significantly from baseline to 12 *** control group showed small but non-significant improvements from 6 months to 12 months(and from baseline to 12 m

关键词： Behavior change maintenance Cancer survivorship Computer tailoring eHealth Physical activityTagedAPTARAEnd

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CETP as a Target in HDL-raising Therapy:lessons from APOE*3-Leiden. CETP Transgenic mice

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中国动脉硬化杂志 2009年第7期17卷 574-575页

作者： Willeke de Haan Jitske de vries-van der Weij Caroline C. van der Hoogt José W.A. van der Hoorn Johannes A. Romijn J. Wouter Jukema Louis m. Havekes Hans m.g. Princen Patrick C.N. Rensen departments of general Internal medicine Endocrinologyand Metabolic Diseases departments of Human genetics TNO-Biosciences Gaubius Laboratory departments of Cardiology Leiden University Medical Center

Background Cardiovascular disease (CVD) is mainly caused by atherosclerosis,for which dyslipidemia (i.e. high (V)LDL-cholesterol (C),high triglycerides and low HDLC is a major risk factor. To reduce the risk to develo... 详细信息

关键词：心血管疾病动脉粥样硬化血脂胆固醇临床分析

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Phylogeny of the industrial relevant,thermophilic genera myceliophthora and Corynascus

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Fungal Diversity 2012年第1期52卷 197-207页

作者： Joost van den Brink Robert A.Samson Ferry Hagen Teun Boekhout Ronald P.de vries CBS-KNAW Fungal Biodiversity Centre P.O.Box 851673508 AD UtrechtThe Netherlands

Species of the genus myceliophthora and its teleomorph Corynascus have attracted increasing interest due to their potential to produce thermostable *** study re-assessed the phylogenetic relationship of 49 isolates of nine species belonging to myceliophthora and *** species,***,was shown not to belong to the genus myceliophthora and should be placed in the genus *** other species belonged to two phylogenetic clusters:mesophilic fungi with the type species *** and ***,and thermophilic fungi with ***,*** and *** phylogenetic data provides no clear separation of the two genera Corynascus and *** avoid confusion in future taxonomic studies,it is proposed that all existing Corynascus species be renamed to myceliophthora,which is the old name and the one more frequently ***,this study identified two groups within the isolates listed as *** and assigned one group(five isolates)to *** based on AFLP analysis and mating *** study provides new insights into the genetic differences within the genus myceliophthora and will therefore be essential for the interpretation of future genomic and physiological studies of these species.

关键词： myceliophthora Corynascus Thermophiles m.heterothallica m.thermophila.Thermophilic fungi multigene phylogeny AFLP analysis mating behavior

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Nurses' views of fundamental relational skills used in clinical practice: a cross-sectional pilot study

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Frontiers of Nursing 2021年第3期8卷 223-232页

作者： Fiona Timmins Jan m.A.de vries Yvonne muldowney Catherine mc Cabe Jacqueline Whelan Sinead Buckley Sandra mcCarthy The School of Nursing and midwifery Trinity CollegeDublin 2Ireland Portlaoise general Hospital BallyroanPortlaoiseCo.LaoisIreland

Objective:Effective communication skills are one of the core competencies of nursing curricula *** are generally regarded as *** our complete trust in the profession,deficiencies and gaps ***,it is not clear to which extent nurses use key communication skills in practice,and whether or not confident in using these skills compounds environmental issues that *** study explored nurse’s confidence and application of relational skills competencies in nursing ***:A 13-item online survey was used to collect ***:Being self-aware on key areas where there was more *** also lacked confidence in exploring the impact of their personal feelings and values on their *** were also less confident on responding appropriately to instances of unsafe or unprofessional practice and using information and communication systems and ***:given the potential impact of poor relational skills on quality client care,an increased emphasis on caring and compassion,and the ever-expanding use of communication technologies,there is a need to explore the need for reflective practice to enhance continuous professional development for nurses to enhance their relational skills.

关键词： communication relational nurse caring nursing competencies technology listening

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