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Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation

World Journal of Gastroenterology 2012年第5期18卷 425-434页

作者： Hiroaki Takeuchi Ya-Nan Zhang dawn a israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura Department of Clinical Laboratory Medicine Kochi Medical School Kohasu Oko-cho Nankoku-city Kochi783-8505 Japan Laboratory Department Beijing Tiantan Hos-pital Affiliated of Capital Medical University Beijing 100050China Department of Internal Medicine Kanmon Medical Center 1-1-1 Ushiroda-cho Shimonoseki Yamaguchi 751-8501 Japan

AIM:To investigate genetic diversity of Helicobacter pylori (***) cell division-related gene A (cdrA) and its effect on the host ***:Inactivation of *** cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent *** property of *** cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,***-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with ***:Genetic diversity of the *** cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,*** all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American *** of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 ***,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the ***,in 5 of the 6 cases possessing a

关键词： IL-8 分泌物核因子κB 酶联免疫吸附试验聚合酶链反应遗传多样性幽门螺杆菌持续性感染

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