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Quantitative Changes in Microtubule Distribution Correlate with Guard Cell Function in Arabidopsis

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Molecular Plant 2012年第3期5卷 716-725页

作者： William R. Eisinger Viktor Kirik Charlotte lewis david W. Ehrhardt Winslow R. Briggs Department of Biology Santa Clara University Santa Clara CA 95053 USA Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA School of Biological Sciences Illinois State University Normal IL 61790 USA

Radially arranged cortical microtubules are a prominent feature of guard cells. We observed guard cells expressing GFP-tubulin （GFP-TUA6） with confocal microscopy and found recognizable changes in the appearance of micro- tubules when stomata open or close （Eisinger et al., 2012）. In the present study, analysis of fluorescence distribution showed a dramatic increase in peak intensities of microtubule bundles within guard cells as stomata open. This increase was correlated with an increase in the total fluorescence that could be attributed to polymerized tubulin, Adjacent pave- ment cells did not show similar changes in peak intensities or integrated fluorescence when stomatal apertures changed. Imaging of RFP-tagged end binding protein 1 （EB1） and YFP-tagged ^-tubulin expressed in the same cell revealed that the number of microtubules with growing ends remained constant, although the total amount of polymerized tubulin was higher in open than in closed guard cells. Taken together, these results indicate that the changes in microtubule array organization that are correlated with and required for normal guard cell function are characterized by changes in micro- tubule clustering or bundling.

关键词： cell differentiation/specialization cytoskeleton fluorescence imaging organelle biogenesis/function Ara- bidopsis

α1-antitrypsin increases interleukin-1 receptor antagonist production during pancreatic islet graft transplantation

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Cellular & Molecular Immunology 2014年第4期11卷 377-386页

作者： Avishag Abecassis Ronen Schuster Galit Shahaf Eyal Ozeri Ross Green david E Ochayon Peleg Rider Eli C lewis Department of Clinical Biochemistry and Pharmacology Faculty of Health Sciences Ben-Gurion University of the Negev Be'er-Sheva Israel

Although islet transplantation for individuals with type I diabetes has been shown to yield superior blood glucose control, it remains inadequate for long-term control. This is partly due to islet injuries and stresses that can lead to beta cell loss. Inhibition of excess IL-1β activity might minimize islet injuries, thus preserving function. The IL-1 receptor antagonist （IL-1Ra）, an endogenous inhibitor of IL-1β, protects islets from cytokine-induced necrosis and apoptosis. Therefore, an imbalance between IL-1β and IL-1Ra might influence the courses of allogeneic and autoimmune responses to islets. Our group previously demonstrated that the circulating serine-protease inhibitor human alpha-1-antitrypsin （hAAT）, the levels of which increase in circulation during acute-phase immune responses, exhibits anti-inflammatory and islet-protective properties, as well as immunomodulatory activity. In the present study, we sought to determine whether the pancreatic islet allograft-protective activity of hAAT was mediated by IL- 1Ra induction. Our results demonstrated that hAAT led to a 2.04-fold increase in IL-1Ra expression in stimulated macrophages and that hAAT-pre-treated islet grafts exhibited a 4.851-fold increase in IL-1Ra transcript levels, which were associated with a moderate inflammatory profile. Unexpectedly, islets that were isolated from IL-1Ra-knockout mice and pre-treated with hAAT before grafting into wild-type mice yielded an increase in intragraft IL-1Ra expression that was presumably derived from infiltrating host cells, albeit in the absence of hAAT treatment of the host. Indeed, hAAT-pre-treated islets generated hAAT-free conditioned medium that could induce IL-1Ra production in cultured macrophages. Finally, we demonstrated that hAAT promoted a distinct phosphorylation and nuclear translocation pattern for p65, a key transcription factor required for IL- 1Ra expression.

关键词： adaptive immunity inflammation innate immunity macrophages tolerance

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集装箱化对散货市场的影响

中国远洋航务 1998年第12期 6-7页

作者： david lewis 豪罗宾逊船务经纪公司

本报告旨在分析日渐增长的集装箱船队对散货市场的影响。众所周知,自亚洲经济危机以来,国际航运市场也未能幸免。散货船的租金水平跌落至十年来的最低点。船东们正经受着现代航运业诞生以来最不稳定的经济因素的考验。随着散货总量的减少。

关键词：集装箱船集装箱化散货船大宗散货集装箱船队集装箱班轮运输集装箱运输经营人国际航运市场集装箱市场

Holes essentials of human anatomy and physiology /

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1000年

作者： david Shier Jackie Butler Ricki lewis.

来源：畅想之星图书光盘版（南... 评论

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在彩云之南,追求爱和真理

国际人才交流 2018年第7期 64-67页

作者： david C.lewis

中文里有个词非常有用,叫作＂不好意思＂,其含义就是＂尴尬的＂。我经常能听到这个词或许是因为我是个外国人,更有可能对我周围的人做一些蠢事,或者做一些令人尴尬的事情。在我众多＂不好意思＂的时刻里,有一次是我第一次去上课,当面对... 详细信息

中文里有个词非常有用,叫作＂不好意思＂,其含义就是＂尴尬的＂。我经常能听到这个词或许是因为我是个外国人,更有可能对我周围的人做一些蠢事,或者做一些令人尴尬的事情。在我众多＂不好意思＂的时刻里,有一次是我第一次去上课,当面对一帮新学生时,我一开口讲话,就开始流鼻血。这帮学生对他们新外教的第一印象就是：一个高高的外国人躺在地上,被很多学生围着一边递纸巾一边讨论该怎么办。

关键词：真理新学生第一印象外国人意思尴尬

A Stochastic Study on the Wicking Phenomena

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Journal of Donghua University(English Edition) 2012年第2期29卷 178-181页

作者：翁鸣 LUKAS david Key Laboratory of Advanced Textile Materials and Manufacturing Technology Ministry of EducationZhejiang Sci-Tech University Faculty of Textile Engineering Technical University of Liberec

A stochastic approach based on a 3D 3-state Potts model combined with Monte Carlo simulation was used to study the equilibrium wicking height of liquids in vertical cylindrical capillaries. The Lifshitz-van der Waals and lewis acid-base theories were adopted to characterize the apolar and polar interactions in the spin system. The evolution of the spin system was driven by the difference in total energy for two successive states. To verify the model, equilibrium wicking height of water, formamide, heptane, and octane in capillaries of different radii were examined and the corresponding computer simulations were implemented. Good agreement was obtained between the simulation and experimental results. It shows the potential of the proposed approach to be applied in this area.

关键词： Potts model Monte Carlo simulation wicking Lifshitz-van der Waals lewis acid-base

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活性染料染色对羊毛损伤的影响

国外纺织技术（化纤．染整．环境保护分册） 1991年第5期 17-21页

作者： david M lewis 屠天民

一、引言对于防缩处理过的和未处理的羊毛,用活性染料都可生产具有优良湿牢度和良好耐光牢度的色泽明亮的产品和三拼色产品。

关键词：羊毛活性染料染色水解损伤

Age dependent expression and distribution of nitric oxide (NO) synthase isoforms in the ovine kidney

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Open Journal of Molecular and Integrative Physiology 2013年第2期3卷 61-70页

作者： Devin W. Davis Megan L. lewis Wei Qi david A. Hart Francine G. Smith Department of Physiology & Pharmacology Alberta Children’s Hospital Research Institute for Child and Maternal Health University of Calgary Calgary Canada Department of Surgery McCaig Institute for Bone and Joint Health University of Calgary Calgary Canada

The aim of the present study was to measure intrarenal spatial and temporal localization of all three nitric oxide synthase (NOS) isoforms in the developing ovine kidney. Reverse transcriptase-polymerase chain reaction (RT-PCR), Western Blot analyses, and in situ hybridization techniques were performed for NOS I -III isoforms in renal tissue obtained from sheep aged ~24 h, one, three, six, and 12 weeks post natally (N = 3). RT-PCR performed on cortical and medullary kidney tissue revealed the presence of all three NOS isoforms from day one to 12 weeks postnatally. NOS I and NOS II mRNA levels were greater in cortex compared to medulla during the first three weeks whereas NOS III mRNA levels were predominantly transcribed within the medulla. In all NOS isoforms, there was a decrease in cortical mRNA levels after three to six weeks. Protein levels confirmed the presence of all three NOS isoforms over the first three months of postnatal life. By demonstrating NOS isoform transcripts to be more abundant in the early post natal period, these findings may provide insight into the age dependent role of NO in modulating kidney function during ontogeny.

关键词： Postnatal Kidney Newborn iNOS eNOS nNOS Nitric Oxide

Epidemiological, Diagnostic and Therapeutic Aspects of Locally Advanced and/or Metastatic Breast Cancers at the Chu-Mel of Cotonou

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Advances in Breast Cancer Research 2022年第4期11卷 195-205页

作者： Sèdjro Raoul Atade Patrice Dangbemey Roger Klipezo Donald Adjagba david Lionel Togbenon Kabibou Salifou Benjamin Hounkpatin Justin lewis Denakpo Mother and Child Department Institute of Nursing and Obstetrical Care (IFSIO) University of Parakou Parakou Benin Mother and Child Department Faculty of Health Sciences (FSS) University of Abomey Calavi Godomey Benin Mother and Child Department Faculty of Medicine (FM) University of Parakou Parakou Benin

Introduction: Breast cancer mortality remains high in most developing countries. Benin Republic does not yet have a technical platform required for the diagnosis and effective management of breast cancer “according to the recommendations”. Objective: To study the epidemiological, diagnostic and therapeutic aspects of locally advanced and/or metastatic breast cancers at the Centre Hospitalier Universitaire-de la Mère et de l’Enfant Lagune (CHU-MEL) of Cotonou from 2018 to 2021. Study Methods: This was a retrospective cross-sectional study with a descriptive purpose, carried out from January 1, 2018 to September 30, 2021 in the Gynaecology-Obstetrics Section of the CHU-MEL of Cotonou. The study population was patients diagnosed with locally advanced and/or metastatic breast cancers. Results: At the end of the study, 63 cases of locally advanced and/or metastatic breast cancer were recorded in 80 cases of breast cancer;a 74.11% prevalence of locally advanced and metastatic breast cancer. The mean age of the patients was 42.6 years. The main reason for consultation was the discovery of a mass in the breast (65.1%). Clinically, an orange peel was noted in 39.6%. Mammography (47.6%) and breast ultrasonography (42.9%) were the reference imaging workups for breast cancer diagnosis. Histopathologically, infiltrating ductal carcinoma (94.1%) was the predominant type with Scarff-Bloom and Richardson (SBR) grade II found in 35.3%. Progesterone and estrogen receptors were present in 42.9%. Mastectomy with axillary node dissection was the surgical treatment performed (80%) after neoadjuvant chemotherapy (39.6%). Only one patient had received radiotherapy. No patient received hormone therapy. Conclusion: Locally advanced and metastatic cancers remain a real public health problem. It is essential that major investments be made in order to improve both their diagnosis and management.

关键词： Cancer Breast Metastasis Ultrasonography Mammography