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HLA-G 3’UTR 14 bp Insertion Is Associated with a Decreased Risk of Developing Human African Trypanosomiasis in the côte d’Ivoire Population

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Open Journal of Genetics 2019年第2期9卷 31-41页

作者： Bernardin Ahouty Mathurin Koffi david courtin Ilboudo Hamidou Didier Sokouri Innocent Abé Laure Gineau Thomas Konan Lingué Kouakou Tidou Abiba Sanogo Enock Matovu Bruno Bucheton Vincent Jamonneau Simon-Pierre N’Guetta Felix Houphouet Boigny University (UFHB) Cocody Abidjan C&#244 te d’Ivoire Jean Lorougnon Guédé University (UJLoG) Daloa C&#244 te d’Ivoire Institut de Recherche pour le Développement (IRD) Mère et Enfant face aux Infections Tropicales Faculté de Pharmacie Université Paris Descartes Sorbonne Paris Cité France centre International de Recherche-Développement sur l’Elevage en Zone Subhumide (cIRDES) Bobo-Dioulasso Burkina Faso Programme National d’élimination de la Trypanosomiase Humaine Africaine Abidjan C&#244 te d’Ivoire School of Veterinary Medicine Makerere University Kampala Uganda Institut de Recherche pour le Développement (IRD) IRD-CIRAD 177 Intertryp Université de Montpellier Montpellier France Institut Pierre Richet Bouaké C&#244 te d’Ivoire

Human African trypanosomiasis (HAT), or sleeping sickness, caused by Trypanosoma brucei gambiense, is associated with diverse clinical outcomes. Host’s genetic factors involved in immunity are potential factors that can regulate infection. Genetic polymorphisms within HLA-G could influence the level of HLA-G expression and therefore play a critical role in infection outcomes. The goal of our study was to investigate the association of 14 bp Indel HLA-G polymorphism with the susceptibility/resistance to HAT. DNA samples were collected from 119 cases, 221 controls and 43 seropositive individuals living in Ivorian HAT foci. The 14 bp Indel polymorphism was determined by PcR. Homozygous individuals for 14 bp insertion had a lower risk of progressing to active HAT (p = 0.012, OR = 0.27, 95% cI: 0.09 - 0.8). Moreover, the frequency of 14 bp insertion homozygous genotype was higher in the seropositive group (11%) than in the HAT cases group (3%) (p = 0.043, OR = 0.27, 95% cI: 0.07 - 0.99), which suggested a protective effect of 14 bp insertion homozygous genotype. Genetic polymorphisms in HLA-G may be associated with a variable risk to develop HAT. The 14 bp insertion appears to favour the occurrence of long-lasting T. b. gambiense latent infections.

关键词： HLA-G Human African Trypanosomiasis Trypanosoma brucei gambiense Genetic Association Study

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Decline in subarachnoid haemorrhage volumes associated with the first wave of the cOVID- 19 pandemic

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Stroke & Vascular Neurology 2021年第4期6卷 542-552,I0026-I0032页

作者： Thanh N Nguyen Diogo c Haussen Muhammad M Qureshi Hiroshi Yamagami Toshiyuki Fujinaka Ossama Y Mansour Mohamad Abdalkader Michael Frankel Zhongming Qiu Allan Taylor Pedro Lylyk Omer F Eker Laura Mechtouff Michel Piotin Fabricio Oliveira Lima Francisco Mont'Alverne Wazim Izzath Nobuyuki Sakai Mahmoud Mohammaden Alhamza R Al-Bayati leonardo Renieri Salvatore Mangiafico david Ozretic Vanessa chalumeau Saima Ahmad Umair Rashid Syed Irteza Hussain Seby John Emma Griffin John Thornton Jose Antonio Fiorot Rodrigo Rivera Nadia Hammami Anna M cervantes-Arslanian Hormuzdiyar H Dasenbrock Huynh le Vu Viet Quy Nguyen Steven Hetts Romain Bourcier Romain Guile Melanie Walker Malveeka Sharma Don Frei Pascal Jabbour Nabeel Herial Fawaz Al-Mufti Atilla Ozcan Ozdemir Ozlem Aykac Dheeraj Gandhi chandril chugh charles Matouk Pascale Lavoie Randall Edgell Andre Beer-Furlan Michael chen Monika Killer-Oberpfalzer Vitor Mendes Pereira Patrick Nicholson Vikram Huded Nobuyuki Ohara Daisuke Watanabe Dong Hun Shin Pedro Sc Magalhaes Raghid Kikano Santiago Ortega-Gutierrez Mudassir Farooqui Amal Abou-Hamden Tatsuo Amano Ryoo Yamamoto Adrienne Weeks Elena A cora Rotem Sivan-Hoffmann Roberto crosa Markus Möhlenbruch Simon Nagel Hosam Al-Jehani Sunil A Sheth Victor S Lopez Rivera James E Siegler Achmad Fidaus Sani Ajit S Puri Anna Luisa Kuhn Gianmarco Bernava Paolo Machi Daniel G Abud Octavio M Pontes-Neto Ajay K Wakhloo Barbara Voetsch Eytan Raz Shadi Yaghi Brijesh P Mehta Naoto Kimura Mamoru Murakami Jin Soo lee Ji Man Hong Robert Fahed Gregory Walker Eiji Hagashi Steve M cordina Hong Gee Roh Ken Wong Juan F Arenillas Mario Martinez-Galdamez Jordi Blasco Alejandro Rodriguez Vasquez Luisa Fonseca M Luis Silva Teddy Y Wu Simon John Alex Brehm Marios Psychogios William J Mack Matthew Tenser Tatemi Todaka Miki Fujimura Roberta Novakovic Jun Deguchi Yuri Sugiura Hiroshi Tokimura Rakesh Khatri Michael Kelly Lissa Peeling Yuichi Murayama Hugh Stephen Winters Johnny Wong Mohamed Teleb Jeremy Payne Hiroki Fukuda Kosuke Miyake Junsuke Shimbo Yusuke Sug Neurology RadiologyBoston Medical CenterBostonMassachusettsUSA Neurology Marcus Stroke&Neuroscience CenterGrady Memorial HospitalEmory University School of MedicineAtlantaGAUSA Radiology Radiation OncologyBoston University School of MedicineBostonMassachusettsUSA Neurology National Hospital Organization Osaka National HospitalOsakaJapan Neurosurgery National Hospital Organization Osaka National HospitalOsakaJapan Neurology Alexandria UniversityAlexandriaEgypt Radiology Boston Medical CenterBostonMassachusettsUSA Department of Neurology Xinqiao HospitalChongqingChina Neurosurgery University of Cape TownRondeboschWestern CapeSouth Africa Neurosurgery Interventional NeuroradiologyClinica La Sagrada FamiliaBuenos AiresArgentina Neuroradiologie Neurologie VasculaireHospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Neurologie Vasculaire Hospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Interventional Neuroradiology Fondation Ophtalmologique Adolphe de RothschildParisÎle-de FranceFrance Neurology Hospital Geral de FortalezaFortalezaBrazil Interventional Neuroradiology Hospital Geral de FortalezaFortalezaBrazil Neuroradiology Nottingham University Hospitals NHS TrustNottinghamUK Department of Neurosurgery Kobe City Medical Center General HospitalKobeHyogoJapan Interventional Neurovascular Unit University Hospital CareggiFirenzeToscanaItaly Neuroradiology University Hospital Centre ZagrebZagrebCroatia Interventional Neuroradiology Hopital BicetreLe Kremlin-BicetreFrance Stroke and Interventional Neuroradiology Lahore General HospitalLahorePakistan Neurological Institute Cleveland Clinic Abu DhabiAbu DhabiUAE Department of Radiology Beaumont HospitalDublinIreland Neurology Stroke UnitHospital-Estadual CentralVitoriaBrazil Neuroradiology Instituto de Neurocirugia Dr AsengoSantiagoChile Interventional Neuroradiology Institut National de NeurologieTunisTunisia Neurology Boston Medical CenterBostonMassachusettsUSA Neurosurgery Boston Medical Center

Background During the cOVID-19 pandemic,decreased volumes of stroke admissions and mechanical thrombectomy were *** study’s objective was to examine whether subarachnoid haemorrhage(SAH)hospitalisations and ruptured aneurysm coiling interventions demonstrated similar *** We conducted a cross-sectional,retrospective,observational study across 6 continents,37 countries and 140 comprehensive stroke *** with the diagnosis of SAH,aneurysmal SAH,ruptured aneurysm coiling interventions and cOVID-19 were identified by prospective aneurysm databases or by International classification of Diseases,10th Revision,*** 3-month cumulative volume,monthly volumes for SAH hospitalisations and ruptured aneurysm coiling procedures were compared for the period before(1 year and immediately before)and during the pandemic,defined as 1 March-31 May *** prior 1-year control period(1 March-31 May 2019)was obtained to account for seasonal *** There was a significant decline in SAH hospitalisations,with 2044 admissions in the 3 months immediately before and 1585 admissions during the pandemic,representing a relative decline of 22.5%(95%cI−24.3%to−20.7%,pclined with 1170-1035 procedures,respectively,representing an 11.5%(95%cI−13.5%to−9.8%,p=0.002)relative *** analysis was noted for aneurysmal SAH hospitalisation decline from 834 to 626 hospitalisations,a 24.9%relative decline(95%cI−28.0%to−22.1%,pcrease in ruptured aneurysm coiling was noted in low coiling volume hospitals of 41.1%(95%cI 32.3%to 50.6%,p=0.008)despite a decrease in SAH admissions in this *** There was a relative decrease in the volume of SAH hospitalisations,aneurysmal SAH hospitalisations and ruptured aneurysm embolisations during the cOVID-19 *** findings in SAH are consistent with a decrease in other emergencies,such as stroke and myocardial infarction.

关键词： diagnosis representing subarachnoid

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基于网络药理学和分子对接探讨党参干预阿尔兹海默症的作用机制

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时珍国医国药 2021年第7期32卷 1559-1562页

作者：韩乐王晶蒙洁陈冬梅康甲超赵盼段永强甘肃中医药大学公共卫生学院甘肃兰州730000 甘肃中医药大学基础医学院甘肃兰州730000

目的基于网络药理学和分子对接技术探讨党参干预阿尔兹海默症的作用机制。方法通过TcMSP数据库检索党参活性化合物并整理其对应的靶标;通过Genecards和OMIM数据库检索阿尔兹海默症相关的靶标。党参活性化合物对应靶标与疾病靶标取交集... 详细信息

目的基于网络药理学和分子对接技术探讨党参干预阿尔兹海默症的作用机制。方法通过TcMSP数据库检索党参活性化合物并整理其对应的靶标;通过Genecards和OMIM数据库检索阿尔兹海默症相关的靶标。党参活性化合物对应靶标与疾病靶标取交集后应用cytoscape3.6.0软件构建党参活性化合物-AD靶标调控网络;利用STRING数据库构建交集靶标相互作用网络,获取此网络的tsv文件,导入cytoscape3.6.0软件对网络进行分析,筛选关键靶标和关键化合物;通过david数据库进行GO分析和KEGG分析,预测关键靶标作用机制。利用AutoDock软件将关键化合物与AD关键蛋白受体进行分子对接,对党参干预AD的作用机制作出合理推测。结果筛选得到党参活性化合物10个,对应靶标101个,筛选出关键靶标SLc6A3、MAOB、PLAU、ADH1c、cHRM1、cHRM2、NR3c2、PTGS2、NFKBIA、TYR、MAPK1、IL6等39个,关键化合物7种。GO分析得到GO条目206条,KEGG分析得到KEGG通路77条。分子对接结果显示Luteolin木樨草素与cASP3、cASP9、HMOX1、IL10、IL6、VEGFA、MAPK1、XIAP、JUN、AKT1等都有良好的结合。结论党参的活性成分可能通过调控HMOX1、AKT1、cASP3、cASP9、IL10、IL6、VEGFA、MAPK1、XIAP、JUN等靶标,G蛋白偶联乙酰胆碱受体活性通路,刺激神经交互作用通路等多个途径发挥干预AD的作用。

关键词：党参阿尔兹海默病网络药理学分子对接

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Ventilation practices in burn patients—an international prospective observational cohort study

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Burns & Trauma 2021年第1期9卷 47-57页

作者： Gerie J.Glas Janneke Horn Markus W.Hollmann Benedikt Preckel Kirsten colpaert Manu Malbrain Ary Serpa Neto Karim Asehnoune Marcello Gamma de Abreu Ignacio Martin-Loeches Paolo Pelosi Folke Sjoerg Jan M.Binnekade Berry cleffken Nicole P.Juffermans Paul Knape Bert G.Loef david P.Mackie Perenlei Enkhbaatar Nadia Depetris Anders Perner Eva Herrero Lucia cachafeiro Marc Jeschke Jeffrey Lipman Matthieu legrand Johannes Horter Athina Lavrentieva Alex Kazemi Anne Berit Guttormsen Frederik Huss Mark Kol Helen Wong Therese Starr Luc De crop Wilson de Oliveira Filho Joa Manoel Silva Junior cintia M.c.Grion Marjorie Burnett Frederik Mondrup Francois Ravat Mathieu Fontaine Renan le Floch Mathieu Jeanne Morgane Bacus Maite chaussard Marcus lehnhardt Bassem Daniel Mikhail Jochen Gille Aidan Sharkey Nicole Trommel Auke c.Reidinga Nadine Vieleers Anna Tilsley Henning Onarheim Maria Teresa Bouza Alexander Agrifoglio Filip Fredén Tina Palmieri Lynda E.Painting Marcus J.Schultz LAMiNAR investigators Academic Medical center University of AmsterdamAmsterdamAZ 1105The Netherlands Department of Anaesthesia and Intensive Therapy Medical University of Lublin Aleje Racklawickie 1–20-059 Lublin–Poland AZ JAN PALFIJN GENT Watersportlaan 5–9000 Gent–Belgium ABc Medical School SaPauloBangúSP 5001Brazil Service d’Anesthésie Réanimation chirurgicale Nantes 44093France University Hospital carl Gustav carus Dresden 01307Germany St James University Hospital Dublin D08 NHY1Ireland University of Genoa GenoaGE 16128Italy Linkoing University Hospital Linkoing 58185Sweden Maasstad Hospital RotterdamDZ 3079The Netherlands Red cross Hospital BeverwijkLE 1942The Netherlands Martini Hospital GroningenNT 9728The Netherlands University of Texas Medical Branch GalvestonTX 77555USA Turin cTO Burn center TurinTO 10126Italy Rigshospitalet Copenhagen 2100Denmark La Paz University Hospital Madrid 28046Spain Ross Tilley Burn centre Sunnybrook Health Sciences CentreToronto M4N 3M5Canada Royal Brisbane and Women’s Hospital Queensland UniversityHerstonQLD 4029Australia Australian and New Zealand Intensive care Research centre.Monash University MelbourneVIC 3004Australia GH St-Louis-Lariboisière APHPParis 75010France BG Klinik Ludwigshafen Ludwigshafen 67071Germany Papanikoalou Hospital Thessaloniki 54621Greece Middlemore Hospital OtahuhuAuckland 2025New Zealand Haukeland University Hospital Bergen 5021Norway Uppsala University Hospital Uppsala 75185Sweden concord Repatriation General Hospital NSW University of SydneyConcord 2139Australia Ziekenhuis Netwerk Antwerpen-Stuivenberg Antwerpen 2060Belgium Universidade de Sao Paulo Sao Paulo 01246-903Brazil Universidade Estadual de Londrina Londrina 86057-970Brazil Sunnybrook Health Sciences centre Toronto M4N 3M5Canada cHU Lyon St Luc Lyon 69007France cHU Nantes Service dánesthesie reanimation chirugicale Nantes 44093France Hopital Roger Salengro CHRU LilleLille 59037France Saint-Louis Hospital Paris 75010France BG University Hospital Bergmannsheil Bo

Background:It is unknown whether lung-protective ventilation is applied in burn patients and whether they benefit from *** study aimed to determine ventilation practices in burn intensive care units(IcUs)and investigate the association between lung-protective ventilation and the number of ventilator-free days and alive at day 28(VFD-28).Methods:This is an international prospective observational cohort study including adult burn patients requiring mechanical *** tidal volume(V_(T))was defined as V_(T)≤8 mL/kg predicted body weight(PBW).levels of positive end-expiratory pressure(PEEP)and maximum airway pressures were *** association between V_(T) and VFD-28 was analyzed using a competing risk *** settings were presented for all patients,focusing on the first day of *** also compared ventilation settings between patients with and without inhalation ***:A total of 160 patients from 28 IcUs in 16 countries were *** V_(T) was used in 74%of patients,median V_(T) size was 7.3[interquartile range(IQR)6.2–8.3]mL/kg PBW and did not differ between patients with and without inhalation trauma(p=0.58).Median VFD-28 was 17(IQR 0–26),without a difference between ventilation with low or high V_(T)(p=0.98).All patients were ventilated with PEEP levels≥5 cmH_(2)O;80%of patients had maximum airway pressurescmH_(2)***:In this international cohort study we found that lung-protective ventilation is used in the majority of burn patients,irrespective of the presence of inhalation *** of low V_(T) was not associated with a reduction in *** registration:*** *** of registration:9 December 2014.

关键词： Mechanical ventilation Inhalation trauma Lung-protective critical care

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The HINT ProjectmExploring the Use of New Technologies in Heritage Interpretation

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Journal of Earth Science and Engineering 2014年第3期4卷 156-174页

作者： Robina R. Barton david cooper Anne Guyomard Sophie. c. Justice Peter Samsom Elizabeth Picket cathrien. L. Posthumus Nathalie cayla Geopark Shetland Shetland Amenity Trust Garthspool Lerwick ZEl ONY UK . Geopark chablais Syndicatlntercommunald Amenagement du Chablais Thonon-Les-Bains 74200 France North Pennines AONB Partnership The Old Co-op Building Stanhope DL13 2UY UK Geopark de Hondsrug Bronnegerstraat 129531 TG Borger The Netherlands ED YTEM (Laboratory of Environment Dynamics and Territories) UMR CNRS-University of Savoy Technolac 73 376 Le Bourgetdu Lac Cedex France

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应用DNA条形码技术鉴定东南沿海蝙蝠种类

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中华卫生杀虫药械 2017年第1期23卷 61-64页

作者：谭伟龙吕恒朱长强胡丹艾乐乐杨露王长军邓小昭南京军区军事医学研究所江苏南京210002

目的探索DNA条形码技术在蝙蝠种类鉴定中的应用。方法基于kimura双参数模型计算23只蝙蝠cyt B序列的种内种间遗传距离。结果种间遗传距离显著大于种内遗传距离,通过NJ法构建系统发育树聚类分析,不同种类的蝙蝠位于不同的分支,同属的蝙... 详细信息

目的探索DNA条形码技术在蝙蝠种类鉴定中的应用。方法基于kimura双参数模型计算23只蝙蝠cyt B序列的种内种间遗传距离。结果种间遗传距离显著大于种内遗传距离,通过NJ法构建系统发育树聚类分析,不同种类的蝙蝠位于不同的分支,同属的蝙蝠分支较不同属的蝙蝠分支较短,种内分支则更短。种间遗传距离均在1.3以上,种内遗传距离均在0.21以下,与NcBI序列比对结果一致,与形态学鉴定结果一致(20只菲菊头蝠,1只马铁菊头蝠,1只大卫鼠耳蝠,1只小黄蝠)。结论 DNA条形码技术对于本研究中所涉及的蝙蝠种类具有较好的区分效果,可以作为一种有效的工具在蝙蝠鉴定中进行应用。

关键词： cytb基因 DNA条形码蝙蝠

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锥体束cT三维测量下颌前徙对颅面型及自然头颈姿势位的作用

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解放军医学院学报 2016年第7期37卷 745-749页

作者：段瑶 Delnaz Patel david E.Morris 赵临平 Pravin K.Patel 刘彦普第四军医大学口腔医学院口腔颌面外科陕西西安710032 伊利诺依大学外科部颅颌面外科中心伊利诺伊州芝加哥60612

目的通过锥体束cT（cone-beam computed tomography,cBcT）数据的三维测量,研究下颌前徙后颅面型、自然头颈姿势位的变化。方法收集2008年3月-2015年3月在伊利诺伊大学颅颌面外科中心接受下颌双侧矢状劈开术（bilateral sagittal split ... 详细信息

目的通过锥体束cT（cone-beam computed tomography,cBcT）数据的三维测量,研究下颌前徙后颅面型、自然头颈姿势位的变化。方法收集2008年3月-2015年3月在伊利诺伊大学颅颌面外科中心接受下颌双侧矢状劈开术（bilateral sagittal split osteotomy,BSSO）前徙的病例25例,男4例,女21例,平均年龄（17.95±1.43）岁。均为安氏二类牙颌面发育性骨性错颌畸形患者。分为A组（单颌BSSO前徙）8例、B组（双颌前徙）12例、c组（双颌前徙＋颏成型）5例。将入选病例术前（T1）、术后1.5～3个月（T2）、术后1～2年（T3）的cBcT数据导入第三方软件进行三维测量并记录T1、T2、T3的颅面型和自然头颈姿势位（natural head posture,NHP）。主要测量指标：1）上颌颅面型测量指标：NSPNS（后鼻棘点PNS与颅底成角）、PNS-S_cP（PNS到三维坐标系冠状面距离）、PNS-S_FH（PNS到坐标系横断面距离）、GPF R_L（双侧腭大孔的空间距离在坐标系横断面上的投影,其变化值代表上颌水平宽度的变化）;2）下颌颅面型测量指标：NSL（下颌正中联合舌侧最凸点L与蝶鞍中心点S连线与颅底所成角度）、L-S_cP（L到坐标系冠状面距离）、L-S_FH（L到坐标系横断面距离）;3）NHP测量指标：NSc2（颅颈角,颅底与第二颈椎轴线所成空间角度在坐标系矢状面上的投影角度）、cIP-S_cP（第二颈椎椎体最后下点cIP与坐标系冠状面的距离）和cSP-S_cP（第二颈椎椎体最后上点cSP与坐标系冠状面的距离）;4）颅面型与NHP关系测量指标：NSc2-NSL（NSc2与NSL两个角度差值）、L-cIP（L与cIP距离在坐标系矢状面的投影）、PNS-cSP（PNS与cSP距离在坐标系矢状面的投影）。分析颅面型和NHP的测量值及两者变化值的相关性。结果颅面型测量指标在T1、T2、T3的测量值差异有统计学意义（P〈0.05）：其中NSPNS（P〈0.001）、NSL（P=0.015）、L-S_cP（P〈0.001）,且L-S_cP在分组间有交互作用（P=0.018）。NHP测量指标cSP-S_cP（P=0.03）在分组间有交互作用。颅面型与NHP关系测量指标在T1、T2、T3的测量值差异有统计学意义（P〈0.05）：其中L-cIP（P=0.005）、PNS-cSP（P〈0.001）。颅下颌角NSL与颅颈角NSc2在T1（r=0.487,P=0.014）、T2（r=0.576,P=0.003）、T3（r=0.675,P〈0.001）时相线性相关。结论下颌前徙可使颅颌面形态和NHP产生变化,在三维坐标系中重叠颅底可以一定程度减少由于NHP变化而导致的颅面型误判,从而获得立体、全面的正颌正畸疗效评估。

关键词：下颌前徙术双侧矢状劈开术自然头颈姿势位颅面型锥体束cT

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利用GO数据库分析与预测乳杆菌肽聚糖对小鼠免疫细胞基因表达的影响

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中国免疫学杂志 2008年第5期24卷 443-447页

作者：孙进乐国伟李永福王周平朱建津吕文平施用晖江南大学食品科学与技术国家重点实验室江南大学食品学院无锡214122

目的:探索乳酸菌肽聚糖对小鼠免疫细胞基因表达的影响。方法:给BALB/c小鼠腹腔注射乳杆菌肽聚糖1次或3次,从腹腔巨噬细胞和脾淋巴细胞合并细胞提取RNA,利用Affymetrix MOE430A基因芯片分析基因表达,利用基于GO数据库的芯片数据分析工具... 详细信息

目的:探索乳酸菌肽聚糖对小鼠免疫细胞基因表达的影响。方法:给BALB/c小鼠腹腔注射乳杆菌肽聚糖1次或3次,从腹腔巨噬细胞和脾淋巴细胞合并细胞提取RNA,利用Affymetrix MOE430A基因芯片分析基因表达,利用基于GO数据库的芯片数据分析工具分析乳杆菌肽聚糖诱导后的代表性GO定义。结果:腹腔注射一次乳杆菌肽聚糖(i·p·)引起了腹腔巨噬细胞和脾脏淋巴细胞基因表达的广泛变化,但特异性不强。注射三次后表达显著改变的基因主要与免疫反应有关。定义两次刺激引起显著变化的所有基因为肽聚糖响应基因,其代表性GO生理过程定义预测主要与巨噬细胞吞噬活力增强、淋巴细胞激活、炎性反应正调节有关。结论:肽聚糖刺激的早期反应涉及先天性免疫激活和抗原呈递过程。

关键词：乳杆菌肽聚糖免疫调节基因表达 cO数据库

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Evading the host response:Staphylococcus“hiding”in cortical bone canalicular system causes increased bacterial burden

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Bone Research 2020年第4期8卷 447-457页

作者： Stephen D.Zoller Vishal Hegde Zachary D.c.Burke Howard Y.Park chad R.Ishmael Gideon W.Blumstein William Sheppard christopher Hamad Amanda H.Loftin Daniel O.Johansen Ryan A.Smith Marina M.Sprague Kellyn R.Hori Samuel J.clarkson Rachel Borthwell Scott I.Simon Jeff F.Miller Scott D.Nelson Nicholas M.Bernthal Department of Orthopedic Surgery University of CaliforniaLos Angeles125016th St Suite 2100Santa MonicaCA 90404USA david Geffen School of Medicine University of CaliforniaLos Angeles10833 Le Conte AveLos AngelesCA 90095USA Department of Internal Medicine University of CaliforniaLos Angeles757 Westwood PlazaSuite 7501Los AngelesCA 90095USA Department of Biomedical Engineering University of CaliforniaDavisDavisCA 95616USA california NanoSystems Institute University of CaliforniaLos Angeles570 Westwood PlazaLos AngelesCA 90095USA Department of Microbiology Immunologyand Molecular GeneticsDavid Geffen School of MedicineUniversity of CaliforniaLos Angeles 90095USA Department of Pathology University of CaliforniaLos Angeles125016th St Suite 3450Santa MonicaCA 90404USA

Extremity reconstruction surgery is increasingly performed rather than amputation for patients with large-segment pathologic bone *** persists as to the optimal void filler for this“limb salvage”surgery,whether metal or allograft *** focus on optimizing important functional gains for patients,and the risk of devastating implant infection has been thought to be similar regardless of implant *** insights into infection pathophysiology are challenging this equipoise,however,with both basic science data suggesting a novel mechanism of infection of Staphylococcus aureus(the most common infecting agent)into the host lacunar–canaliculi network,and also clinical data revealing a higher rate of infection of allograft over *** current translational study was therefore developed to bridge the gap between these insights in a longitudinal murine model of infection of allograft bone and ***-time Staphylococci infection characteristics were quantified in cortical bone vs metal,and both microarchitecture of host implant and presence of host immune response were *** orders-of-magnitude higher bacterial burden was established in cortical allograft bone over both metal and cancellous *** establishment of immune-evading microabscesses was confirmed in both cortical allograft haversian canal and the submicron canaliculi network in an additional model of mouse femur bone *** study results reveal a mechanism by which Staphylococci evasion of host immunity is possible,contributing to elevated risks of infection in cortical *** presence of this local infection reservoir imparts massive clinical implications that may alter the current paradigm of osteomyelitis and bulk allograft infection treatment.

关键词： immunity insight reservoir

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基于网络药理学探究肉桂治疗慢性肾脏病的作用机制

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中医学报 2020年第6期35卷 1271-1277页

作者：李若颀夏天卫陈晨施乐周国威刘金柱刘昱江沈计荣南京中医药大学附属医院/江苏省中医院江苏南京210029

目的:采用网络药理学方法建立“药效成分-疾病靶标-生物学通路”之间的关系,筛选肉桂治疗慢性肾脏病的作用靶点,明确其作用机制。方法:采用中药系统药理学成分分析平台(BATMAN-TcM)数据库获得肉桂的活性化合物,利用Pubchem数据库,获取... 详细信息

目的:采用网络药理学方法建立“药效成分-疾病靶标-生物学通路”之间的关系,筛选肉桂治疗慢性肾脏病的作用靶点,明确其作用机制。方法:采用中药系统药理学成分分析平台(BATMAN-TcM)数据库获得肉桂的活性化合物,利用Pubchem数据库,获取化合物的SMIleS结构,将SMIleS结构导入SwissTargetPrediction平台,预测活性成分的潜在靶点,再通过Genecards数据库获得相关化合物靶点,取两者交集得到肉桂作用靶点。通过TTD、DrugBank、OMIM、GAD、DisGeNET数据库获得cKD疾病相关靶点,并与肉桂作用靶点取交集。利用STRING数据库及cytoscape3.2.1软件构建靶点相互作用网络图,并利用cytoHubba插件的Mcc算法筛选关键靶点;基于david数据库对靶点进行GO富集和KEGG富集分析。结果:共得到肉桂活性化合物18个,肉桂-cKD交集靶点29个。经过Mcc算法筛选得到关键基因10个,包括雌激素受体alpha、人环加氧酶1、表皮生长因子受体、血管内皮生长因子受体、基质金属蛋白酶9、基质金属蛋白酶2、成纤维细胞生长因子受体1、丝氨酸蛋白酶抑制剂-1、DNA多聚酶B、多药耐药性蛋白1、B细胞淋巴瘤/白血病-2。GO富集共118个结果,主要包括细胞对缺氧的反应、丝裂原活化蛋白激酶活性的正调节、血管生成的正调节、炎症反应、磷脂酶c活性的正调节、蛋白自体磷酸化、对白细胞介素-1的细胞反应、血管内皮生长因子受体信号通路的正调节等。KEGG富集相关通路共51条,包括NF-кB信号通路、PI3K-Akt信号通路、TNF信号通路、Ras信号通路、Rap1信号通路等。结论:通过网络药理学初步预测肉桂在慢性肾脏病治疗中改善糖脂代谢与胰岛素抵抗、抗氧化、抗炎与免疫调节、抗凝、促进成血管生成、改善组织局部血供等潜在作用机制,为下一步研究提供理论依据。

关键词：肉桂慢性肾脏病网络药理学作用机制

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