Neurogenesis takes place in the adult mammalian brain in three areas:Subgranular zone of the dentate gyrus(DG);subventricular zone of the lateral ventricle;olfactory *** molecular markers can be used to characterizeth...
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Neurogenesis takes place in the adult mammalian brain in three areas:Subgranular zone of the dentate gyrus(DG);subventricular zone of the lateral ventricle;olfactory *** molecular markers can be used to characterizethe cells involved in adult *** has been recently suggested that a population of bone marrow(BM)progenitor cells may migrate to the brain and differentiate into neuronal *** explore this hypothesis,we injected recombinant SV40-derived vectors into the BM and followed the potential migration of the transduced ***-term BM-directed gene transfer using recombinant SV40-derived vectors leads to expression of the genes delivered to the BM firstly in circulating cells,then after several months in mature neurons and microglial cells,and thus without central nervous system(CNS)*** of transgene-expressing cells expressed NeuN,a marker of mature ***,BM-derived cells may function as progenitors of CNS cells in adult *** mechanism by which the cells from the BM come to be neurons remains to be *** the observed gradual increase in transgene-expressing neurons over 16mo suggests that the pathway involved differentiation of BM-resident cells into neurons,cell fusion as the principal route cannot be totally ruled *** studies using similar viral vectors showed that BM-derived progenitor cells migrating in the CNS express markers of neuronal precursors or immature ***-positive cells were found in the subgranular zone of the DG of the hippocampus 16 mo after intramarrow injection of the *** addition to cells expressing markers of mature neurons,transgene-positive cells were also positive for nestin and doublecortin,molecules expressed by developing neuronal *** cells were actively proliferating,as shown by short term BrdU incorporation *** seizures by using kainic acid increased the number of BM progenitor cells transduced by SV40vectors migrating
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