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Biological efficacy of perpendicular type-I collagen protruded from TiO_(2)-nanotubes

International Journal of Oral Science 2020年第4期12卷 346-355页

作者： Chia-Yu Chen david M.Kim Cliff Lee john Da Silva Shigemi Nagai Toshiki Nojiri Masazumi Nagai Department of Oral Medicine Infection and ImmunityHarvard School of Dental Medicine188 Longwood AvenueBostonMAUSA Department of Restorative Dentistry and Biomaterials Sciences Harvard School of Dental Medicine188 Longwood AvenueBostonMAUSA Department of Prosthodontics and Oral Implantology School of Dental MedicineIwate Medical University19-1 UchimaruMoriokaIwateJapan

The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen(Col-I)from TiO2 nanotubes(NT-EPF surface).We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating plateletmediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly,mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental *** surface modification was accomplished in two ***,TiO2 nanotubes(NT)array was fabricated via *** and depths of NTs were controlled by applied voltage and ***,an electrophoretic fusion(EPF)method was applied to fuse Col-I into nanotube arrays in a perpendicular *** wettability was assessed by contact angle *** bioactivity of modified TiO2 surfaces was evaluated in terms of NIH3T3 fibroblast attachment,platelet activation,and collagen *** attachment,aggregation,and activation of platelets as well as release of platelet-related growth factors were demonstrated on NTEPF ***-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape.A direct linkage between pseudopod-like processes of fibroblasts to NTEPF surfaces was ***,the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular *** the limitation of this in vitro study,the TiO2 nanotube with perpendicular Col-I surface(NT-EPF)promoted better cell attachment,induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal.

关键词： activation linkage sized

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Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis

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World Journal of Gastroenterology 2016年第19期22卷 4662-4672页

作者： david Coyle Justin M Murphy Brian Doyle Anne Marie O’Donnell john Gillick Prem Puri Temple Street Children's University Hospital Dublin 1 Ireland National Children's Research Centre Our Lady's Children's Hospital Dublin 12 Ireland

AIM: To determine if expression of colonic tryptophan hydroxylase-2(TPH2), a surrogate marker of neuronal 5-hydroxytryptamine, is altered in Hirschsprung's-associated enterocolitis. METHODS: Entire resected colonic specimens were collected at the time of pull-through operation in children with Hirschsprung's disease(HSCR, n = 12). Five of these patients had a history of pre-operative Hirschsprung's-associated enterocolitis(HAEC). Controls were collected at colostomy closure in children with anorectal malformation(n = 10). The distribution of expression of TPH2 was evaluated using immunofluorescence and confocal microscopy. Protein expression of TPH2 was quantified using western blot analysis in the deep smooth muscle layers. RESULTS: TPH2 was co-expressed in nitrergic and cholinergic ganglia in the myenteric and submucosal plexuses in ganglionic colon in HSCR and healthy controls. Co-expression was also seen in submucosal interstitial cells of Cajal and PDGFRα+ cells. The density of TPH2 immuno-positive fibers decreased incrementally from ganglionic bowel to transition zonebowel to aganglionic bowel in the myenteric plexus. Expression of TPH2 was reduced in ganglionic bowel in those affected by pre-operative HAEC compared to those without HAEC and healthy controls. However, expression of TPH2 was similar or high compared to controls in the colons of children who had undergone diverting colostomy for medically refractory ***: Altered TPH2 expression in colonic serotonergic nerves of patients with HSCR complicated by HAEC may contribute to intestinal secretory and motor disturbances, including recurrent HAEC.

关键词： Serotonin Tryptophan hydroxylase 2 Hirschsprung Enterocolitis Ganglionic

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Redo sphincteroplasty:are the results sustainable?

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Gastroenterology Report 2016年第1期4卷 39-42,I0002页

作者： Kwangdae Hong Giovanna Dasilva john T.Dollerschell david Maron Steven D.Wexner Department of Colorectal Surgery Cleveland Clinic FloridaWestonFLUSA

Objective:This study aimed to investigate the long-termoutcomes of patients who undergo redo sphincteroplasty(RS).Methods:Patients with fecal incontinence(FI)who underwent RS between November 1988 and December 2011 were retrospectively identified from a prospective database.A questionnaire and telephone survey assessed current Cleveland Clinic Fecal Incontinence Score(CCFFIS;best 0,worst 20)and Fecal Incontinence Quality of Life(FIQoL;best 4.1,worst 1)*** was defined as no further continence surgery,no stoma and CCFFIS<9 at completion of *** Wilcoxon and Mann-Whitney U tests were used for comparing quantitative *** logistic regression analysis was done to identify predictive factors for ***:Fifty-six(66.7%)of 84 patients who underwent RS were available for evaluation at a median follow-up of 74(range:12-283)*** mean CCFFIS decreased from 16.563.7 to 11.966.6(P<0.001)at last *** patients(21.4%)underwent further continence surgery for failed sphincteroplasty,three(5.4%)of whom had a permanent *** patients(32.1%)had a CCFFIS<9 at the completion of follow-up,and 16(28.6%)had long-term *** patients evaluated for FIQoL had a mean value of 2.6(range:1.0-4.1).Postoperative CCFFIS was correlated with FIQoL(Spearman’s correlation coefficient?0.854,P<0.001).Logistic regression analysis did not reveal any significant predictive variables for success of ***:Based on our criteria for success,the long-term success rate for RS over a median of 74 months is poor.

关键词： fecal incontinence redo sphincteroplasty long-term outcome

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Ecometrics:The traits that bind the past and present together

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Integrative Zoology 2010年第2期5卷 88-101页

作者： Jussi T.ERONEN P.david POLLY Marianne FRED john DAMUTH david C.FRANK Volker MOSBRUGGER Christoph SCHEIDEGGER Nils Chr.STENSETH Mikael FORTELIUS Department of Geosciences and Geography Helsinki UniversityFinland Department of Geological Sciences Indiana UniversityUSA ARONIA Research Institute atÅbo Akademi University and Novia University of Applied SciencesCoastal Zone Research TeamEkenäFinland Department of Ecology Evolution and Marine BiologyUniversity of CaliforniaSanta BarbaraCaliforniaUSA Swiss Federal Research Institute WSL BirmensdorfSwitzerland Senckenberg Natural History Museum and Research Institute FrankfurtGermany Centre for Ecological and Evolutionary Synthesis(CEES) Department of BiologyUniversity of OsloOsloNorway

We outline here an approach for understanding the biology of climate change,one that integrates data at multiple spatial and temporal ***-free trait analysis,or“ecometrics,”is based on the idea that the distribution in a community of ecomorphological traits such as tooth structure,limb proportions,body mass,leaf shape,incubation temperature,claw shape,any aspect of anatomy or physiology can be measured across some subset of the organisms in a *** of temporal or spatial scale,traits are the means by which organisms interact with their environment,biotic and *** measures these interactions by focusing on traits which are easily measurable,whose structure is closely related to their function,and whose function interacts directly with local *** trait distributions are thus a comparatively universal metric for exploring systems dynamics at all *** main challenge now is to move beyond investigating how future climate change will affect the distribution of organisms and how it will impact ecosystem services and to shift the perspective to ask how biotic systems interact with changing climate in general,and how climate change affects the interactions within and between the components of the whole biotic-physical *** believe that it is possible to provide believable,quantitative answers to these *** of this we have initiated an IUBS program iCCB(integrative Climate Change Biology).

关键词： community structure ecometrics integrative Climate Change Biology Taxon-free trait distribution

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Standard method design considerations for semi-quantification of total naphthenic acids in oil sands process affected water by mass spectrometry： A review

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Frontiers of Chemical Science and Engineering 2017年第3期11卷 497-507页

作者： Kevin A. Kovalchik Matthew S. MacLennan Kerry M. Peru john V. Headley david D.Y. Chen Department of Chemistry University of British Columbia Vancouver BC V6T 1Z1 Canada Watershed Hydrology and Ecology Research Division Water Science and Technology Directorate Science and Technology Branch Environment and Climate Change Canada Saskatoon SK S7N 3H5 Canada

Naphthenic acids are a complex class of thousands of naturally occurring aliphatic and alicyclic carboxylic acids found in oil sands bitumen and in the wastewater generated from bitumen processing. Dozens of analytical methods have been developed for the semiquantification of total naphthenic acids in water samples. However, different methods can give different results, prompting investigation into the comparability of the many methods. A review of important methodological features for analyzing total naphthenic acids is presented and informs the design of future standard methods for the semi- quantification of total naphthenic acids using mass spectrometry. The design considerations presented are a synthesis of discussions from an Environment and Climate Change Canada （ECCC） led taskforce of 10 laboratory experts from government, industry and academia during April 2016 and subsequent discussions between University of British Columbia and ECCC representatives. Matters considered are： extraction method, solvent, pH, and temperature; analysis instrumentation and resolution; choice of calibration standards; use of surrogate and internal standards; and use of online or offline separation prior to analysis. The design considerations are amenable to both time-of-flight and Orbitrap mass spectrometers.

关键词： total naphthenic acids environmental samples oil sands process affected water polar organics mass spectrometry

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Using on-site liver 3-D reconstruction and volumetric calculations in split liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2016年第6期15卷 587-592页

作者： Trevor W Reichman Brittany Fiorello Ian Carmody Humberto Bohorquez Ari Cohen john Seal david Bruce George E Loss Ochsner Multi-Organ Transplant Institute Ochsner Medical Center

BACKGROUND： Split liver transplantation increases the number of grafts available for transplantation. Pre-recovery assessment of liver graft volume is essential for selecting suitable recipients. The purpose of this study was to determine the ability and feasibility of constructing a 3-D model to aid in surgical planning and to predict graft weight prior to an in situ division of the donor liver. METHODS： Over 11 months, 3-D volumetric reconstruction of 4 deceased donors was performed using Pathfinder Scout liver volumetric software. Demographic, laboratory, operative, perioperative and survival data for these patients along with donor demographic data were collected prospectively and analyzed retrospectively. RESULTS： The average predicted weight of the grafts from the adult donors obtained from an in situ split procedure were 1130 g （930-1458 g） for the extended right lobe donors and 312 g （222-396 g） for left lateral segment grafts. Actual adult graft weight was 92% of the predicted weight for both the extended right grafts and the left lateral segment grafts. The predicted and actual graft weights for the pediatric donors were 176 g and 210 g for the left lateral segment grafts and 308 g and 280 g for the extended right lobe grafts,respectively. All grafts were transplanted except for the right lobe from the pediatric donors due to the small graft ***： On-site volumetric assessment of donors provides useful information for the planning of an in situ split and for selection of recipients. This information may expand the donor pool to recipients previously felt to be unsuitable due to donor and/or recipient weight.

关键词： split liver transplantation reduced-size liver transplantation 3-D reconstruction

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Crop Phenomics and High-Throughput Phenotyping:Past Decades,Current Challenges,and Future Perspectives

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Molecular Plant 2020年第2期13卷 187-214页

作者： Wanneng Yang Hui Feng Xuehai Zhang Jian Zhang john H.Doonan William david Batchelor Lizhong Xiong Jianbing Yan Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research Huazhong Agricultural UniversityWuhan 430070P.R.China National Key Laboratory of Wheat and Maize Crops Science/College of Agronomy Henan Agricultural UniversityZhengzhou 450002P.R.China The National Plant Pheno mics Centre In stitute of BiologicalEnviron mental and Rural SciencesAberystwyth UniversityAberystwythUK Department of Biosystems Engineering Auburn UniversityAuburnALUSA These authors contributed equally to this article.

Since whole-genome sequencing of many crops has been achieved,crop functional genomics studies have stepped into the big-data and high-throughput ***,acquisition of large-scale phenotypic data has become one of the major bottlenecks hindering crop breeding and functional genomics ***,recent technological advances provide us potential solutions to relieve this bottleneck and to explore advanced methods for large-scale phenotyping data acquisition and processing in the coming *** this article,we review the major progress on high-throughput phenotyping in controlled environments and field conditions as well as its use for post-harvest yield and quality assessment in the past *** then discuss the latest multi-omics research combining high-throughput phenotyping with genetic ***,we propose some conceptual challenges and provide our perspectives on how to bridge the phenotype-genotype *** is no doubt that accurate high-throughput phenotyping will accelerate plant genetic improvements and promote the next green revolution in crop breeding.

关键词： crop phenomics high-throughput field phenotyping root system architecture yield and quality genetic studies

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Calcinosis circumscripta in a captive African cheetah(Acinonyx jubatus)

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Asian Pacific Journal of Tropical Biomedicine 2014年第10期4卷 832-834页

作者： Chisoni Mumba david Squarre Maxwel Mwase john Yabe Tomoyuki Shibahara University of Zambia School of Veterinary MedicineBox 32379Lusaka 10101Zambia Zambia Wildlife Authority(ZAWA) Private Bag 1ChilangaZambia National Institute of Animal Health 3-1-5 KannondaiTsukubaIbaraki 305-0856Japan

This article reports a first case of calcinosis circumscripta in a captive African cheetah(Acinonyx jubatus).Histopathology demonstrated well defined multiple cystic structures containing granular,dark basophilic materials with peripheral granulomatous reaction,characterized by presence of multinucleated giant cells surrounded by a varying amounts of fibrous connective *** staining with von Kossa revealed black stained deposits confirming the presence of calcium salts.

关键词： Calcinosis circumscripta von Kossa Cheetah Zambia

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Micro/nano Indentation and Single Grit Diamond Grinding Mechanism on Ultra Pure Fused Silica

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Chinese Journal of Mechanical Engineering 2011年第6期24卷 963-970页

作者： ZHAO Qingliang GUO Bing STEPHENSIN david CORBETT john School of Mechatronics Engineering Harbin Institute of Technology Harbin 150001 China Materials Department Cranfield University Cranfield Bedford MK43 OAL UK School of Applied Sciences Cranfield University Cranfield Bedford MK43 OAL UK

The existing research about ductile grinding of fused silica glass was mainly focused on how to carry out ductile regime material removal for generating very ＂smoothed＂ surface and investigate the machining-induced damage in the grinding in order to reduce or eliminate the subsurface *** brittle/ductile transition behavior of optical glass materials and the wear of diamond wheel are the most important factors for ductile grinding of optical *** this paper,the critical brittle/ductile depth,the influence factors on brittle/ductile transition behavior,the wear of diamond grits in diamond grinding of ultra pure fused silica（UPFS） are investigated by means of micro/nano indentation technique,as well as single grit diamond grinding on an ultra-stiff machine tool,Tetraform ＂C＂.The single grit grinding processes are in-process monitored using acoustic emission（AE） and force dynamometer *** wear of diamond grits,morphology and subsurface integrity of the machined groves are examined with atomic force microscope（AFM） and scanning electron microscope（SEM）.The critical brittle/ductile depth of more than 0.5 μm is *** compared to the using roof-like grits,by using pyramidal diamonds leads to higher critical depths of scratch with identical grinding ***,the influence of grit shapes on the critical depth is not significant as *** grinding force increased linearly with depth of cut in the ductile removal regime,but in brittle removal regime,there are large fluctuations instead of forces *** SEM photographs of the cross-section profile show that the median cracks dominate the crack patterns beneath the single ***,The SEM photographs show multi worn patterns of diamond grits,indicating an inhomogeneous wear mechanism of diamond grits in grinding of fused silica with diamond grinding *** proposed research provides the basal technical theory for improving the ultra-precision grinding of UPFS.

关键词： ultra pure fused silica (UPFS) micro/nano indentation single grit diamond grinding ductile material removal subsurface integrity diamond grits wear

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Is there a role for serum endothelin in predicting the severity of acute pancreatitis?

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 290-293页

作者： john Bennett david Cooper Anita Balakrishnan Michael Rhodes Michael Lewis Department of Surgery Norfolk and Norwich University Hospital Colney LaneNorwichNorfolkNR4 7UYEngland

BACKGROUND: Acute pancreatitis remains a common presentation to acute surgical units and carries significant morbidity and mortality. The progression of the disease to necrotizing pancreatitis and multi-organ dysfunction syndrome (MODS) is associated with a very poor clinical outcome, and persistendy high mortality. Increases in serum endothelin (ET) have been seen in animal models of acute pancreatitis and this study aims to investigate whether there is a change in serum ET-1 in patients with acute pancreatitis and whether any such change is linked to disease severity. METHODS: All patients admitted with acute pancreatitis were prospectively recruited from die emergency admissions at the Norfolk and Norwich University Hospital. Serum ET levels were determined on admission, at 24 hours and 5 days post admission. Healthy adult controls were recruited from dermatology outpatients. RESULTS: A total of 21 patients joined the trial after giving informed consent. There were 3 men and 18 women with a median age of 65 years (range 26-87 years). Serum ET levels were significantly higher in acute pancreatitis patients than in normal controls (P <0. 05). An association was seen between persistendy raised serum ET levels and progression to MODS. CONCLUSIONS: The study does demonstrate a correlation between the circulating levels of ET and acute pancreatitis in humans, although it does not elicit its involvement in the pathogenesis of the disease. The observation that a persistendy high level of circulating ET-1 is associated with progression to MODS may indicate a role for ET in the monitoring of acute pancreatitis patients for recovery or progression to MODS.

关键词： endothelin acute pancreatitis

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