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Scoring of sperm chromosomal abnormalities by manual and automated approaches：qualitative and quantitative comparisons

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Asian j.urnal of Andrology 2010年第2期12卷 257-262页

作者： Helen G. Tempest Siu Yan Cheng david j. gillott Alan H. Handyside Alan R. Thomhill Darren K. Griffin London Bridge Fertility Gynaecology and Genetics Centre London SEI 9RY UK Bridge Genoma London SE1 9RY UK Department of Biosciences University of Kent Canterbury Kent CT2 7N J UK

It is now well known that levels of sperm disomy correlate to levels of infertility （as well as other factors）.The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sperm inj.ction （ICSI） has become a hotly debated issue in assisted reproduction;however,there remain barriers to the practical implementation of offering sperm disomy screening in a clinical setting. The maj.r barrier is the operator time taken to analyze a statistically meaningful （sufficient） number of cells. The introduction of automated ＇spot counting＇software-hardware combinations presents a potential solution to this problem. In this preliminary validation study,we analyzed 10 patients,both manually and using a commercially available spot counter. Results show a statistically significant correlation between both approaches for scoring of sperm disomy,but no correlation is found when scoring for diploid sperm. The most likely explanation for the latter is an apparent overscoring of two closely associated sperm heads as a single diploid cell. These results,and similar further studies that will ensue,help to inform cost-benefit analyses that individual clinics need to carry out in order to decide whether to adopt sperm aneuploidy screening as a routine tool for the assessment of sperm from men requiring ICSI treatment.

关键词： aneuploidy assisted reproductive technologies automation fluorescent in situ hybridization methods scoring spermatozoa

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子宫内膜异位症患者血浆和腹膜液中结合珠蛋白β链亚型的表达

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世界核心医学期刊文摘（妇产科学分册） 2005年第9期1卷 20-21页

作者： Ferrero S. gillott D.j. Remorgida V. 朱国栋 Reproductive Phys iology Laboratory St. Bartholomew's Sch. Med. Dent. St. Bartholomew's Hospit al 48-53 St. Bartholomew's Close West Smithfield London EC1A 7BE United Kingdom Dr.

Obj.ctive: To estimate the expression of haptoglobin (Hp)-βchain isoforms in the peritoneal fluid (PF) and plasma (PL) of women with and without endometrios is. Design: Cross-sectional study. Setting: University hospital. Patient(s): Se venty-two patients with endometriosis and 35 controls. Intervention(s): Periton eal fluid and PL samples were subj.cted to two-dimensional gel electrophoresis, silver stained, digitally captured, and compared by semiquantitative computeriz ed analysis. Main Outcome Measure(s): Expression of Hp βchain isoforms. Result( s): No significant difference was observed in the frequency of expression of the Hp βchain isoforms between the two groups. One βchain isoform (HpβE; molecul ar weight, 38.40 ±0.94 kD; and isoelectric point, 5.63 ±0.17) had significantl y higher expression in both the PF and PL of women with endometriosis than in th e PF and PL of controls. No significant difference was observed in HpβE express ion between women with mild (revised American Fertility Society [rAFS], stage I -II) and severe (rAFS, stage III-IV) endometriosis. In the control group, Hpβ E expression was correlated with the phase of the menstrual cycle. Conclusion(s) : Women with endometriosis have higher PF and PL levels of HpβE. Further invest igation should be aimed at producing antibodies against the HpβE-specific epit opes to determine whether its measurement may improve the clinical diagnosis of endometriosis.

关键词：子宫内膜异位症结合珠蛋白 β链美国生育学会二维凝胶电泳大学医院横断面研究等电点无显著性差异确诊率

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