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Soil fungal and prokaryotic community structure exhibits differential short-term responses to timber harvest in the Pacific Northwest

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Pedosphere 2020年第1期30卷 109-125页

作者： Rachel E.dANIELSON Megan L.MCGINNIS Scott M.HOLUB david d.myrold department of Crop and Soil Science Oregon State UniversityCorvallis OR 97331-7306(USA) United State Forest Service Helena-Lewis and Clark National Forest2880 Skyway DriveHelena MT 59602(USA) Weyerhaeuser Company PO Box 275Springfield OR 97477(USA) department of Land Airand Water ResourcesUniversity of CaliforniaDavis CA 95616USA

Conventional clear-cut timber harvest is a widespread industrial practice across the Pacific Northwest;however,information regarding how these practices impact soil microbial community structure at the regional scale is *** evidence of consistent and substantial impact of harvest on soil microbial functional profiles across the region(despite a range of environmental conditions),the objective of this study was to determine the extent to which harvest also influences the structure of prokaryotic and fungal soil microbial communities,and how generalized these trends are throughout the geographic *** soil samples were collected one year before and after harvest across nine second-growth douglas-fir forests in the Pacific *** community dNA was extracted from the soils,and high-throughput targeted gene sequencing of the 16 S r RNA gene for prokaryotes and the internal transcribed spacer(ITS)gene for fungi was *** diversity was consistently and significantly higher after harvest;it was moderately so for fungal communities(+14.6%),but only marginally so for prokaryotic communities(+2.0%).Similarly,on average,a greater proportion of the variation in the community structure of fungi(20.1%)at each site was associated with forest harvest compared to that of prokaryotes(13.2%).Overall,the greatest influence of timber harvest on soil microbial communities appeared to be a relative depletion of ectomycorrhizal fungi,with a concomitant enrichment of saprotrophic *** the short-term responses of soil microbial communities across the region,particularly those of tree root-associated symbionts,may aid our understanding of the role soil microbial communities play in ecological succession.

关键词： clear-cut douglas-fir forest ectomycorrhizal fungi forest soil microbial community microbial diversity root-associated symbiont saprotrophic fungi

Evidence for niche differentiation of nitrifying communities in grassland soils after 44 years of different field fertilization scenarios

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Pedosphere 2020年第1期30卷 87-97页

作者： Zhongjun JIA Xue ZHOU Weiwei XIA dario FORNARA Baozhan WANG Elizabeth Anne WASSON Peter CHRISTIE Martin F.POLZ david d.myrold State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai UniversityNanjing 210098(China) Jiangsu Key Laboratory of Agricultural Meteorology College of Applied MeteorologyNanjing University of Information Science and TechnologyNanjing 210044(China Agri-Food and Biosciences Institute Belfast BT95PX(UK) department of Civil and Environmental Engineering Massachusetts Institute of TechnologyCambridge MA 02139(USA) department of Crop and Soil Science Oregon State UniversityCorvallis OR 97331(USA)

Long-term nitrogen(N)fertilization imposes strong selection on nitrifying communities in agricultural soil,but how a progressively changing niche affects potentially active nitrifiers in the field remains poorly *** a 44-year grassland fertilization experiment,we investigated community shifts of active nitrifiers by dNA-based stable isotope probing(SIP)of field soils that received no fertilization(CK),high levels of organic cattle manure(HC),and chemical N fertilization(CF).Incubation of dNA-SIP microcosms showed significant nitrification activities in CF and HC soils,whereas no activity occurred in CK *** 44 years of inorganic N fertilization selected only 13C-ammonia-oxidizing bacteria(AOB),whereas cattle slurry applications created a niche in which both ammonia-oxidizing archaea(AOA)and AOB could be *** analysis indicated that Nitrosospira sp.62-like AOB dominated inorganically fertilized CF soils,while Nitrosospira sp.41-like AOB were abundant in organically fertilized HC *** 13C-AOA in HC soils were affiliated with the 29i4 *** 13C-nitrite-oxidizing bacteria(NOB)were dominated by both Nitrospira-and Nitrobacter-like communities in CF soils,and the latter was overwhelmingly abundant in HC *** 13C-labeled nitrifying communities in SIP microcosms of CF and HC soils were largely similar to those predominant under field *** results provide direct evidence for a strong selection of distinctly active nitrifiers after 44 years of different fertilization regimes in the *** findings imply that niche differentiation of nitrifying communities could be assessed as a net result of microbial adaption over 44 years to inorganic and organic N fertilization in the field,where distinct nitrifiers have been shaped by intensified anthropogenic N input.

关键词： ammonia-oxidizing archaea ammonia-oxidizing bacteria community shift long-term fertilization nitrite-oxidizing bacteria nitrogen enrichment stable isotope probing microcosm

Soil Organic Carbon in a Changing World

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Pedosphere 2017年第5期27卷 789-791页

作者： JIA Zhongjun Yakov KUZYAKOV david.myrold James TIEdJE State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) Agro-Technology Institute RUDN UniversityMoscow 115419(Russia) Institute of Physicochemical and Biological Problems in Soil Science RASPushchino 142290(Russia) department of Crop and Soil Science Oregon State UniversityCorvallis OR 97881(USA) Center for Microbial Ecology Michigan State UniversityEast Lansing MI 48824-1325(USA)

Soil contains more than three times as much carbon (C) as either the atmosphere or terrestrial *** organic C (SOC) is essentially derived from inputs of plant and animal residues,which are processed by the microbiota (bacteria,archaea,protists,fungi and viruses) that dominates SOC transformation and turnover in complex terrestrial environments.A

关键词：土壤有机碳世界陆地植被生物处理古细菌碳含量 SOC 微生物

Making bridges between preclinical and clinical insights into age-related cognitive decline

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Neural Regeneration Research 2025年第8期20卷 2321-2322页

作者： david VC Brito Clévio Nóbrega Algarve Biomedical Center-Research Institute(ABC-RI) Campus GambelasFaroPortugal Algarve Biomedical Center-(ABC) Campus GambelasFaroPortugal Faculty of Medicine and Biomedical Sciences(FMCB) University of AlgarveGambelas CampusFaroPortugal

With people living longer,the societal impact of age-related cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increasingly important to comprehend the cognitive shifts linked to aging-whether th... 详细信息

关键词： bridges becoming insight

Women in visual neural regeneration research

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Neural Regeneration Research 2025年第2期20卷 489-490页

作者： Tonia S.Rex david J.Calkins Vanderbilt Eye Institute Vanderbilt University Medical CenterNashvilleTNUSA

The year 2024 marks the 60^(th)anniversary of Title IX and 25 years since the New York Times revealed bias against female faculty members at the Massachusetts Institute of *** take an opportunity here to examine the state of gender bias in a relatively new yet already prominent field,neural regeneration in the visual system,for which there is a well-defined context useful for this *** National Eye Institute(NEI)provided the first round of research funding for its Audacious Goals Initiative(AGI)on visual neural regeneration in 2013 and the last round in ***,we focus on this *** sources included PubMed,the National Science Foundation(NSF),the NEI,the Blue Ridge Institute for Medical Research and data from the major professional organization for eye and vision research,the Association for Research in Vision and Ophthalmology(ARVO).

关键词： neural visual Times

ESTIMATING CROSS-TRAINING CALL CENTER CAPACITY THROUGH SIMULATION

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Journal of Systems Science and Systems Engineering 2016年第4期25卷 448-468页

作者： david A. Munoz Nathaniel d. Bastian department of Industrial and Manufacturing Engineering The Pennsylvania State University

Call centers have grown world-wide during the past decade. One of the most important aspects considered by call center managers is the optimization of its operators, which implies covering the highly variable demand and f＇mding an efficient way to assign people to certain shifts in order to achieve a desirable service level and abandonment rate. Another challenge is determining which system setup is appropriate for the specific call center. Should we have a single-skill call center or multi-skill call center？ If we do have the latter, how many multi-skill agents should we have on staff？. In this case study, we generate and analyze discrete-event systems simulation-optimization models to test the behavior of a real-world call center under the actual configuration and under different levels of cross-training. The model results help call center managers by： 1） determining the optimal number of operators needed for different staff configtmations in order to achieve the targets for service level and abandonment; 2） providing information about the trade-off between the key measurements in the call center; and 3） providing useful information about the number of operators needed and used for each hour of operation to estimate the number of four-hour shifts required to achieve the performance targets. Our experimental f＇mdings from this case study suggest that a bi-skill call center is economically better in the long-rtm compared to a full-skill or single-skill call center. This case study augments the call center body of knowledge by providing additional managerial insights for the operations management community.

关键词： Call centers cross-training discrete-event systems simulation simulation optimization service operations service systems

An investigation of roof runoff during rain events at the Royal Military College of Canada and potential discharge to Lake Ontario

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Journal of Environmental Sciences 2011年第7期23卷 1072-1078页

作者： david G. Kelly Ron d. Weir Steven d. White Analytical Sciences Group & SLOWPOKE-2 Facility at RMC Department of Chemistry and Chemical Engineering Royal Military College of Canada 11 General Crerar Crescent

The Royal Military College of Canada, located on the north eastern shore of Lake Ontario, possesses an abundance of copper roofs and lacks surface water treatment prior to discharge into Lake Ontario. Rainwater, roof runoff and soil samples were collected and analyzed for copper and other parameters. Copper was consistently detected in runoff samples with average concentrations of 3200 ± 2100 μg/L. Multivariable linear regression analysis for a dependant copper runoff concentration yielded an adjusted R 2 value of 0.611, based on an independent variable model using minimum temperature, maximum temperature, total precipitation, and wind speed. Lake water samples taken in the vicinity of storm water outfalls draining areas with copper roofs ranged from 2.0 to 40 μg/L copper. Such data exceed the 2.0 μg/L Canadian Water Quality Guidelines for the Protection of Aquatic Life as outlined by the Canadian Council of Ministers of the Environment （CCME）. Analysis of raw, filtered and digested forms suggested that the majority of copper present in runoff and lake water samples was in a dissolved form. The majority of soils taken in this study displayed copper concentrations below the 63 μg/g CCME residential/parkland land use limits. These findings suggested that ion exchange processes between runoff water and soil do not occur to a sufficient extent to elevate copper levels in soil. It may therefore be concluded that the eventual fate of copper, which is not discharged via storm water outfalls, is lost to the water table and Lake Ontario through the sub-soil.

关键词： copper roof runoff Lake Ontario Canadian water quality guidelines for the protection of aquatic life

Rapid micro-immunohistochemistry

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Microsystems & Nanoengineering 2020年第1期6卷 285-294页

作者： Robert d.Lovchik david Taylor Govind Kaigala IBM Research Europe Saeumerstrasse 48803 RueschlikonSwitzerland Present address:Eidgenossische Technische Hochschule Zurich Department of Mechanical and Process EngineeringSonneggstrasse 38092 ZurichSwitzerland

We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue ***(IHC)comprises a sequence of specific biochemical reactions and allows the detection of specific proteins in tissue *** the rapid implementation of IHC,we fabricated horizontally oriented microfluidic probes(MFPs)with functionally designed apertures to enable square and circular footprints,which we employ to locally expose a tissue to time-optimized sequences of different *** show that the two main incubation steps of IHC protocols can be performed on MdAMB468-1510A cell block sections in less than 30 min,compared to incubation times of an hour or more in standard *** analysis on the timescale of tens of minutes could potentially be applied during surgery,enabling clinicians to react in more dynamically and ***,this rapid IHC implementation along with conservative tissue usage has strong potential for the implementation of multiplexed assays,allowing the exploration of optimal assay conditions with a small amount of tissue to ensure high-quality staining results for the remainder of the sample.

关键词： versatile steps sections

Performance of activated carbon coated graphite bipolar electrodes on capacitive deionization method for salinity reduction

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Frontiers of Environmental Science & Engineering 2020年第6期14卷 87-96页

作者： Hossein d.Atoufi Hasti Hasheminejad david J.Lampert School of Civil and Environmental Engineering Oklahoma State UniversityStillwaterOK 74078USA department of Civil Engineering Isfahan University of TechnologyIsfahan 84156-83111Iran

This research investigates a capacitive deionization method for salinity reduction in a batch reactor as a new approach for *** of cost and energy compared with conventional desalination methods are the significant advantages of this *** this research,experiments were performed with a pair of graphite bipolar electrodes that were coated with a one-gram activated carbon *** completing preliminary tests,the impacts of four parameters on electrical conductivity reduction,including(1)the initial concentration of feed solution,(2)the duration of the tests,(3)the applied voltage,and(4)the pH of the solution,were *** results show that the maximum efficiency of electrical conductivity reduction in this laboratory-scale reactor is about 55%.Furthermore,the effects of the initial concentration of feed solution are more significant than the other ***,using the capacitive deionization method for water desalination with low and moderate salt concentrations(i.e.,brackish water)is proposed as an affordable *** with conventional desalination methods,capacitive deionization is not only more efficient but also potentially more environmentally friendly.

关键词： Capacitive deionization(CdI) desalination Electrical conductivity(EC) Graphite bipolar electrode Activated carbon coated(ACC)