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The new Caribbean Nitrogen Index to assess nitrogen dynamics in vegetable production systems in southwestern Puerto Rico

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International Soil and Water Conservation Research 2017年第1期5卷 69-75页

作者： Miguel Oliveras-Berrocales david Sotomayor-Ramirez Jorge A.Delgado Luis R.Perez-Alegriad USDA-NRCS Arkansas:2603 Federal Building100 E8th AvePine BluffAR 71601USA University of Puerto Rico Mayaguez CampusCollege of Agricultural SciencesDepartment of Agroenvironmental SciencesPO Box 9000MayagüezPR 00680USA USDA-ARS Acting Research LeaderSoil Management and Sugar Beet Research Unit2150 Centre Ave.Building DSuite 100Fort CollinsCO 80526USA University of Puerto Rico Mayaguez CampusCollege of Agricultural SciencesDepartment of Agricultural and Biosystems EngineeringUSA

Nutrient loss from agricultural fields is one of the main factors influencing surface-and ground-water *** fertilizer nitrogen(N)consumption rates in vegetable production systems and horticultural crops in Puerto Rico fluctuate between 112 and 253 kg N/*** nitrogen use efficiency of vegetable crops is low,increasing the potential for nitrogen losses and high residual soil nitrate *** of residual soil N and N losses to the environment can be a difficult *** models such as the USDA-ARS N Index can be used to identify the relative magnitude of varying N-loss pathways and to identify best management *** studies were conducted to quantify residual soil N and crop N removal,and to validate the Nitrogen Index in onion,tropical pumpkin and tomato production systems in the Lajas Valley in southwestern Puerto *** between observed and simulated values were determined to examine the capability of the model for evaluating N *** was good correlation between observed and predicted values for residual soil N(r=0.88)and crop N removal(r=0.99)(p<0.05).In the production systems evaluated,the N volatilization losses ranged from 1 to 4 kg N/ha,the denitrification losses ranged from 18 to 46 kg N/ha,the leaching losses ranged from 155 to 779 kg N/ha,and the residual soil nitrate ranged from 64 to 401 kg N/*** N use efficiency ranged from 15% to 39%.The results obtained showed that the Nitrogen Index tool can be a useful tool for evaluating N transformations in vegetable production systems of Puerto Rico's semi-arid zone.

关键词： Nitrogen index Nitrogen management Residual soil N Crop N removal

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Resolution of Crohn's disease and complex regional pain syndrome following treatment of paratuberculosis

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World Journal of Gastroenterology 2015年第13期21卷 4048-4062页

作者： J Todd Kuenstner William Chamberlin Saleh A Naser Michael T collins Coad Thomas Dow John M Aitken Stuart Weg Grzegorz Telega Kuruvilla John david Haas Torsten M Eckstein Maher Kali Christine Welch Thomas Petrie Department of Pathology Charleston Area Medical CenterCharlestonWV 25304United States Department of Gastroenterology Mountain View Regional HospitalLas CrucesNM 88011United States Burnett School of Biomedical Sciences UCF College of MedicineOrlandoFL 32816United States Department of Pathobiological Sciences the University of Wisconsin School of Veterinary MedicineMadisonWI 53706United States 5 Damon Street Eau ClaireWI 54701United States Otakaro Pathways Ltd. 63 Bibiana StreetChristchurch 8011New Zealand Patients Medical Alternative Pain Management800 Second Avenue Suite 900New York CityNY 10017United States Children’s Hospital of Wisconsin 8915 W.Connell Ave.MilwaukeeWI 53226United States Department of Neurosciences Charleston Area Medical CenterCharlestonWV 25304United States Department of Chemistry University of CharlestonCharlestonWV 25304United States Department of Microbiology Colorado State UniversityImmunology and PathologyCO 80523United States CAMC Clinical Trials Center 3100 Mac Corkle Avenue S.E.Suite 806CharlestonWV 25304United States CAMC Clinical Trials Center CharlestonWV 25304United States AVIcure Bioscience LLC Superior Quartz ProductsBethlehemPA 18020United States

A cohort of family members with various chronic diseases including Crohn's disease, asthma, complex regional pain syndrome, hypothyroidism, type 1 diabetes mellitus, and lymphangiomatosis and/or evidence of infection by Mycobacterium avium subsp. paratuberculosis(MAP) are described in this series of case reports. MAP was cultured from the blood of three members affected by the first five diseases and there was accompanying elevated anti-MAP Ig G in two members. The patient affected by the sixth disease has a markedly elevated anti-MAP titer. The two patients affected by the first four diseases have been treated with a combination of anti-MAP antibiotics and ultraviolet blood irradiation therapy with resolution of the disease symptomatology and inability to culture MAP in post treatment blood samples. These case reports of patients with MAP infections provide supportive evidence of a pathogenic role of MAP in humans.

关键词： Crohn’s disease Complex regional pain syndrome Lym

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FK506对实验性卒中疗效的系统评价和Meta分析

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中国循证医学杂志 2006年第6期6卷 430-438页

作者： Malcolm R Macleod Tori O’collins Laura L Horky david W Howells Geoffrey A Donnan 段建钢 National Stroke Research Institute Melbourne Australia School of Molecular and Clinical MedicineUniversity of EdinburghEdinburghUK National Stroke Research Institute Melbourne Australia National Stroke Research Institute Melbourne Australia Department of MedicineUniversity of MelbourneMelbourneAustralia 四川大学华西医院神经内科

FK506是治疗急性卒中的一种候选用药。决定一种药物是否可以用于临床试验，应当以全面的、无偏倚的动物实验数据的评估为依据，同时还应考虑到这些数据的局限性。这种评估不但应包括药物疗效，而且也应包括药效在体内的特征和局限性。... 详细信息

FK506是治疗急性卒中的一种候选用药。决定一种药物是否可以用于临床试验，应当以全面的、无偏倚的动物实验数据的评估为依据，同时还应考虑到这些数据的局限性。这种评估不但应包括药物疗效，而且也应包括药效在体内的特征和局限性。本研究应用系统评价和Meta分析的方法对FK506在卒中动物模型中保护作用的证据进行评价。总共纳入了29个描述了实验步骤的研究，包括1759只动物。结果显示，FK506疗效的点估计值（结局指标的改善）是31．3％[95％CI（0．272-0．354）]。在采用氯胺酮麻醉和短暂性脑缺血的动物实验中，FK506的疗效更高，在使用大鼠，合并其他疾病的动物及仅以梗死面积为疗效指标的实验中，FK506疗效较低。已发表的实验研究质量均接近临床试验标准，但在高质量的研究中，FK506的疗效较低。FK506在实验性脑卒中的研究中，虽然显示出有明显的疗效，但是应注意由于研究质量和可能的发表偏倚等冈素的影响，FK506的疗效可能被过高估计。

关键词： FK506 Meta分析神经保护卒中系统评价

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天然气管道

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商业周刊（中文版） 2017年第23期 90-91页

作者： Tim Loh Ryan collins

阿巴拉契亚地区的天然气即将被大量输出大卫·菜因伦德（david Rheinlander）家住美国宾夕法尼亚州西南，他曾经梦想在自家房子后而的树林里建一个小木屋。如今57岁的他从自家后院向外望去，在他原先设想建小木屋的地方，看到的却是一... 详细信息

阿巴拉契亚地区的天然气即将被大量输出大卫·菜因伦德（david Rheinlander）家住美国宾夕法尼亚州西南，他曾经梦想在自家房子后而的树林里建一个小木屋。如今57岁的他从自家后院向外望去，在他原先设想建小木屋的地方，看到的却是一排被砍掉的树木和堆得高高的钢管。过去六个月里，建筑工人在他的房子后面开出了一条路。这条路宽约30米，穿过连绵的山峰，向西延伸大约1130公里，经过邻近的俄亥俄州，一直通到密歇根州。

关键词：天然气管道阿巴拉契亚地区宾夕法尼亚州建筑工人俄亥俄州密歇根州木屋房子

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禽流感病毒H5N1血凝素蛋白的细胞膜上提纯和结晶

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中国医师杂志 2009年第5期11卷 584-586,590页

作者：谌资郑煜煌 Yipu Lin david J Stevens Steve A Wharton Patrick J collins Junfeng Liu Alan J Hay 中南大学湘雅二医院感染科湖南长沙410011 WHO Influenza Collaborating Centre National Institute for Medical Research NW7 1AA London UK Division of Virology and Molecular Biology National Institute for Medical Research NW7 1AA London UK

目的从细胞膜上提纯禽流感病毒H5N1的血凝素蛋白H51151F和H51151F＋A134V＋E186D，并使蛋白结晶。方法细胞内大量增殖重组牛痘病毒，去垢剂提取细胞膜中的HA蛋白，连续蔗糖密度梯度超速离心，Western印迹检测，菠萝蛋白酶等裂解HA，离... 详细信息

目的从细胞膜上提纯禽流感病毒H5N1的血凝素蛋白H51151F和H51151F＋A134V＋E186D，并使蛋白结晶。方法细胞内大量增殖重组牛痘病毒，去垢剂提取细胞膜中的HA蛋白，连续蔗糖密度梯度超速离心，Western印迹检测，菠萝蛋白酶等裂解HA，离子交换层析，SDS—PAGE电泳并染色检测蛋白纯度，坐滴气象扩散法结晶。结果从细胞膜中提取了高纯度的血凝素蛋白H51151F和H51151F＋A134V＋E186D，并得到了H51151F蛋白晶体。结论首次获得了禽流感病毒H5N1的I-IS1151F蛋白晶体，为进一步研究禽流感病毒人传人的可能性打下基础。

关键词：流感禽流感病毒A型鸟/分离和提纯血凝素糖蛋白类流感病毒/分离和提纯

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星际探索卓瑞尔天文台“第一道光”射电天文馆

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室内设计与装修 2023年第6期 70-73页

作者： Julian Gitsham Gary collins Adam Atraktzi Oliver Kampshoff Sarah Willats Clive Lewis Kaja Swiezewska Trang Dao david Brown(摄影) Hassell 不详

“第一道光”射电天文馆由建筑事务所Hassell设计。该项目建成开放后,世界各地的访客在著名的卓瑞尔天文台的参观体验得到很大提升,他们将射电天文学理论,转化成了迷人的星际探索。作为这块场地上最大的公共建筑,展馆的设计以捕捉太空... 详细信息

“第一道光”射电天文馆由建筑事务所Hassell设计。该项目建成开放后,世界各地的访客在著名的卓瑞尔天文台的参观体验得到很大提升,他们将射电天文学理论,转化成了迷人的星际探索。作为这块场地上最大的公共建筑,展馆的设计以捕捉太空爱好者的好奇心为主旨,引发人们科学探索的欲望,在广袤无垠太空收获天文学的发现。

关键词：建筑事务所射电天文学公共建筑广袤无垠瑞尔天文馆天文台好奇心

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Environmental temperatures,physiology and behavior limit the range expansion of invasive Burmese pythons in southeastern USA

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Integrative Zoology 2012年第3期7卷 271-285页

作者： Elliott R.JACOBSON david G.BARKER Tracy M.BARKER Richard MAULDIN Michael L.AVERY Richard ENGEMAN Stephen SECOR College of Veterinary Medicine University of FloridaGainesvilleFlorida Vida Preciosa International(VPI) BoerneTexas US Department of Agriculture Wildlife ServicesNational Wildlife Research CenterFort CollinsColorado US Department of Agriculture Wildlife ServicesNational Wildlife Research CenterGainesvilleFlorida Department of Biological Sciences University of AlabamaTuscaloosaAlabamaUSA

A well-established population of Burmese pythons resides in the Everglades of southern *** in part by a report that identified much of southern USA as suitable habitat for expansion or establishment of the Burmese python,we examined the plausibility of this snake to survive winters at sites north of the *** integrated daily low and high temperatures recorded from October to February from 2005-2011 at Homestead,Orlando and Gainesville,Florida;and Aiken,South Carolina,with minimum temperatures projected for python digestion(16℃),activity(5℃)and survival(0℃).Mean low and high temperatures decreased northward from Homestead to Aiken and the number of days of freezing temperatures increased *** was impaired or inhibited for 2 months in the Everglades and up to at least 5 months in Aiken,and activity was increasingly limited northward during these *** of overwinter survivorship document that a single bout of low and freezing temperatures results in python *** capacity for Burmese pythons to successfully overwinter in more temperate regions of the USA is seemingly prohibited because they lack the behaviors to seek refuge from,and the physiology to tolerate,cold *** tropical Southeast Asia is the source of the Everglades Burmese pythons,we predict it is unlikely that they will be able to successfully expand to or colonize more temperate areas of Florida and adjoining states due to their lack of behavioral and physiological traits to seek refuge from cold temperatures.

关键词： ambient temperature Burmese python invasion snake thermal tolerance

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双极经尿道手术中葡萄糖作为冲洗液的吸收标记物

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麻醉与镇痛 2013年第5期9卷 83-89页

作者： david Piros, MD Tim Fagerstro, MD Justin W. collins, MD Robert G. Hahn, MD, PhD 邓希锦(译) 李文志(校) Departments of Anesthesiology Departments of Urology Karolinska Institutet at Soder Hospital Stockholm Sweden Departments of Bristol Urological Institute Bristol UK Departments of Clinical Research Center Sodertalje Hospital Sodertalje Sweden 不详

背景过去，血清钠离子浓度的降低是诊断经尿道前列腺切除术（transurethralresectionoftheprostate，TURP）冲洗液（不含电解质）吸收的诊断标准。在使用双极的TURP中，冲洗液包含了电解质，所以不能依赖血钠浓度来判断冲洗液是否吸收... 详细信息

背景过去，血清钠离子浓度的降低是诊断经尿道前列腺切除术（transurethralresectionoftheprostate，TURP）冲洗液（不含电解质）吸收的诊断标准。在使用双极的TURP中，冲洗液包含了电解质，所以不能依赖血钠浓度来判断冲洗液是否吸收。在本研究中，我们观察了在使用双极的TURP手术中葡萄糖能否成为冲洗液吸收的诊断标准。方法选取250例使用单极行TURP手术的患者，术中膀胱冲洗液为1．5％的甘氨酸或5％的葡萄糖溶液，检测血糖和血钠浓度。选取10名志愿者，持续输注含1％葡萄糖的醋酸林格液（20ml／kg）30分钟，进行葡萄糖代谢动力学分析。计算机通过采集的数据模拟出不同的吸收模式，以列线图总结出葡萄糖浓度和输注液体容量的关系。结果在TURP中5％葡萄糖灌洗液冲洗后，血钠降低，血糖升高，呈明显的负线性关系（r2=0．80）。在冲洗过程中血糖逐渐升高，从4．6（标准差0．4）mmol／L升高至8．3（0．9）mmol／L。无论何种吸收模式均显示体内摄取1L含1％葡萄糖的冲洗液血糖相应的升高3．7（SD1．6）mmol／L，摄取2L冲洗液血糖升高6．9（1．7）mmol／L。结论在使用单极的TURP中，通过检测血钠浓度来观察冲洗液吸收情况，与之相比较，在使用双极的TURP中，含有电解质的冲洗液中加入1％的葡萄糖，可以成为检测冲洗液吸收情况的标记物。

关键词：经尿道前列腺切除术冲洗液吸收葡萄糖溶液标记物手术中双极 TURP手术血糖升高

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A robust new metric of phenotypic distance to estimate and compare multiple trait differences among populations

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Current Zoology 2012年第3期58卷 426-439页

作者： Rebecca SAFRAN Samuel FLAXMAN Michael KOPP Darren E. IRWIN Derek BRIGGS Matthew R. EVANS W. Chris FUNK david A. GRAY Eileen A. HEBETS Nathalie SEDDON Elizabeth SCORDATO Laurel B. SYMES Joseph A. TOBIAS david P. L. TOEWS J. Albert C. UY Department of Ecology and Evolutionary Biology University of Colorado Boulder CO 80309 U.S.A. Mathematics and B iosciences Group Faculty of Mathematics University of Vienna A-1090 Vienna Austria Current address Evolutionary Biology and Modeling Group Faculty of Sciences Aix-Marseille University 13331 Marseille France. Department of Zoology University of British Columbia 6270 University Boulevard Vancouver BC Canada V6T 1Z4. Research ＆ Evaluation Methodology School of Education University of Colorado Boulder CO 80309 U.S.A. School of Biological and Chemical Sciences Queen Mary University of London London E1 4NS Department of Biology Colorado State University Fort Collins CO 80523 U.S.A. Department of Biology California State University Northridge CA 91330 U.S.A. School of Biological Sciences University of Nebraska Lincoln NE 68588 U.S.A. Edward Grey Institute Department of Zoology University of Oxford Oxford OX1 3PS UK Committee on Evolutionary Biology The University of Chicago Chicago IL 60637U.S.A. Department of Biology Dartmouth College Hanover NH 03755 U.S.A. Department of Biology University of Miami Coral Gables FL 33146 U.S.A.

Whereas a rich literature exists for estimating population genetic divergence, metrics of phenotypic trait divergence are lacking, particularly for comparing multiple traits among three or more populations. Here, we review and analyze via simula- tion Hedges＇ g, a widely used parametric estimate of effect size. Our analyses indicate that g is sensitive to a combination of unequal trait variances and unequal sample sizes among populations and to changes in the scale of measurement. We then go on to derive and explain a new, non-parametric distance measure, ＇Aft＇, which is caiculated based upon a joint cumulative distribution function （CDF） from all populations under study. More precisely, distances are measured in terms of the percentiles in this CDF at which each population＇s median lies. Ap combines many desirable features of other distance metrics into a single metric; namely, compared to other metrics, p is relatively insensitive to unequal variances and sample sizes among the populations sam- pied. Furthermore, a key feature of Ap--and our main motivation for developing it--is that it easily accommodates simultaneous comparisons of any number of traits across any number of populations. To exemplify its utility, we employ Ap to address a ques- tion related to the role of sexual selection in speciation： are sexual signals more divergent than ecological traits in closely related taxa？ Using traits of known function in closely related populations, we show that traits predictive of reproductive performance are indeed, more divergent and more sexually dimorphic than traits related to ecological adaptation [Current Zoology 58 （3）： 426-439 2012].

关键词： Effect size Phenotype divergence Sexual dimorphism Sexual selection Speciation

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禽流感病毒H5N1血凝素蛋白的细胞膜上提纯和结晶

禽流感病毒H5N1血凝素蛋白的细胞膜上提纯和结晶

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中华医学会第四次全国艾滋病、病毒性丙型肝炎暨全国热带病学术会议

作者：谌资郑煜煌 Yipu Lin david J Stevens Steve A Wharton Patrick J collins Junfeng Liu Alan J Hay 中南大学湘雅二医院感染科世界卫生组织流感合作中心

目的从细胞膜上提纯禽流感病毒H5N1的血凝素蛋白H5 I151F和H5 I151F+A134V+E186D,并使蛋白结晶。方法细胞内大量增殖重组牛痘病毒,去垢剂提取细胞膜中的HA蛋白,连续蔗糖密度梯度超速离心,Western印迹检测,菠萝蛋白酶等裂解HA,离子交换层... 详细信息

目的从细胞膜上提纯禽流感病毒H5N1的血凝素蛋白H5 I151F和H5 I151F+A134V+E186D,并使蛋白结晶。方法细胞内大量增殖重组牛痘病毒,去垢剂提取细胞膜中的HA蛋白,连续蔗糖密度梯度超速离心,Western印迹检测,菠萝蛋白酶等裂解HA,离子交换层析,SDS-PAGE电泳并染色检测

关键词：禽流感病毒细胞膜

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