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Exa.ina.ion of the molecula. control of rumina. epithelia. function in response to dieta.y restriction a.d subsequent compensa.ory growth in ca.tle

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Journa. of a.ima. Science a.d Biotechnology 2017年第1期8卷 107-118页

作者： Emma.O＇Shea.Sinea. M. Wa.ers Ka.e Keogh a.a. K. Kelly david a. kenny School of a.riculture a.d Food Science University College DublinBelfield Dublin 4Ireland a.ima. a.d Bioscience Resea.ch Depa.tment Animal & Grassland Research and Innovation Centre Teagasc Grange Dunsany Co Meath Ireland UCD Ea.th Institute University College Dublin Belfield Dublin 4 Ireland

Ba.kground： The objective of this study wa. to investiga.e the effect of dieta.y restriction a.d subsequent compensa.ory growth on the rela.ive expression of genes involved in vola.ile fa.ty a.id tra.sport, meta.olism a.d cell prolifera.ion in rumina. epithelia. tissue of beef ca.tle. Sixty Holstein Friesia. bulls（mea. liveweight 370 ± 35 kg;mea. a.e 479 ± 15 d） were a.signed to one of two groups：（i） restricted feed a.lowa.ce（RES; n = 30） for 125 d（Period 1） followed by a. libitum a.cess to feed for 55 d（Period 2） or（ii） a. libitum a.cess to feed throughout（a.LIB; n = 30）. Ta.get growth ra.e for RES wa. 0.6 kg/d during Period 1. a. the end of ea.h dieta.y period, 15 a.ima.s from ea.h trea.ment group were sla.ghtered a.d rumina. epithelia. tissue a.d liquid digesta.ha.vested from the ventra. sa. of the rumen. Rea.-time q PCR wa. used to qua.tify m RNa.tra.scripts of 26 genes a.socia.ed with rumina. epithelia. function. Vola.ile fa.ty a.id a.a.ysis of rumen fluid from individua. a.ima.s wa. conducted using ga. ***： Diet × period intera.tions were evident for genes involved in ketogenesis（BDH2, P = 0.017）, pyruva.e meta.olism（LDHa. P = 0.048; PDHa., P = 0.015） a.d cellula. tra.sport a.d structure（DSG1, P = 0.019; Ca.T, P = 0.027）. Rumina. concentra.ions of propionic a.id（P = 0.018） a.d n-va.eric a.id（P = 0.029） were lower in RES a.ima.s, compa.ed with a.LIB, throughout the experiment. There wa. a.so a.strong tendency（P = 0.064）towa.d a.diet × period intera.tion for n-butyric with higher concentra.ions in RES a.ima.s, compa.ed with a.LIB, during Period ***： These da.a.suggest tha. following nutrient restriction, the structura. integrity of the rumen wa.l is compromised a.d there is upregula.ion of genes involved in the production of ketone bodies a.d brea.down of pyruva.e for cellula. energy. These results provide a. insight into the potentia. molecula. mecha.isms regula.ing rumina. epithelia. a.sorptive meta.olism a.d growth following nutri

关键词： Beef ca.tle Compensa.ory growth Feed efficiency Nutrient restriction Rumen epithelium

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Ma.ing bridges between preclinica. a.d clinica. insights into a.e-rela.ed cognitive decline

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Neura. Regenera.ion Resea.ch 2025年第8期20卷 2321-2322页

作者： david VC Brito Clévio Nóbrega a.ga.ve Biomedica. Center-Resea.ch Institute(a.C-RI) Campus GambelasFaroPortugal a.ga.ve Biomedica. Center-(a.C) Campus GambelasFaroPortugal Fa.ulty of Medicine a.d Biomedica. Sciences(FMCB) University of AlgarveGambelas CampusFaroPortugal

With people living longer,the societa. impa.t of a.e-rela.ed cognitive decline is becoming more pronounced(Crimmins,2015).Thus,it is increa.ingly importa.t to comprehend the cognitive shifts linked to a.ing-whether th... 详细信息

关键词： bridges becoming insight

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Women in visua. neura. regenera.ion resea.ch

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Neura. Regenera.ion Resea.ch 2025年第2期20卷 489-490页

作者： Tonia.S.Rex david J.Ca.kins Va.derbilt Eye Institute Vanderbilt University Medical CenterNashvilleTNUSA

The yea. 2024 ma.ks the 60^(th)a.niversa.y of Title IX a.d 25 yea.s since the New York Times revea.ed bia. a.a.nst fema.e fa.ulty members a. the Ma.sa.husetts Institute of *** ta.e a. opportunity here to exa.ine the sta.e of gender bia. in a.rela.ively new yet a.rea.y prominent field,neura. regenera.ion in the visua. system,for which there is a.well-defined context useful for this *** Na.iona. Eye Institute(NEI)provided the first round of resea.ch funding for its a.da.ious Goa.s Initia.ive(a.I)on visua. neura. regenera.ion in 2013 a.d the la.t round in ***,we focus on this *** sources included PubMed,the Na.iona. Science Founda.ion(NSF),the NEI,the Blue Ridge Institute for Medica. Resea.ch a.d da.a.from the ma.or professiona. orga.iza.ion for eye a.d vision resea.ch,the a.socia.ion for Resea.ch in Vision a.d Ophtha.mology(a.VO).

关键词： neura. visua. Times

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Oxygen a.d hydrogen isotope ra.ios of chert from the Sixtymile Forma.ion in Gra.d Ca.yon Na.iona. Pa.k,USa.a.wa.m pa.a.oclima.e,freshwa.er deposit

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a.ta.Geochimica 2017年第2期36卷 250-259页

作者： Ra. kenny Geosciences Depa.tment Fort Lewis College Durango CO 81301 USA

New oxygen a.d hydrogen isotope ra.ios of chert from middle, intra.orma.iona. breccia., a.d upper breccia.members of the Sixtymile Forma.ion（SMF） in ea.tern Gra.d Ca.yon Na.iona. Pa.k（a.） yield pa.a.oclima.e estima.es between 27 a.d 33℃. The isotopic compositions of cherts define a.doma.n a.proxima.ely pa.a.lel to the meteoric wa.er line when plotted on a.δD–δ-（18）O dia.ra.; these da.a.indica.e tha. meteoric wa.er wa. involved during forma.ion of the chert. In thin section, the a.sence of interlocking mega.qua.tz（〉35 lm） a.d silica.illed fra.tures a.d veins, a.ong with preserved micromorphologica. silica.fa.rics, suggest tha. the chert ha. not been permea.ed by la.er hydrotherma. fluids. Petrogra.hic observa.ions in thin section such a. cyclic silica.precipita.ion pha.es a.d gla.bula. micromorphologic fa.rics lend support to the interpreta.ion tha. meteoric wa.ers were involved during chert precipita.ion. The post 742 Ma.SMF ha. been correla.ed with dia.ictite（tra.sition） beds of the Kingston Pea. Forma.ion（Ca., which in turn ha.e been interpreted to ha.e been deposited during the Sturtia. Ice a.e（-750–700 Ma.. a.sence of fa.etted a.d stria.ed cla.ts a.d other dia.nostic gla.iogenic fea.ures in the SMF,a. unconforma.le conta.t with the stra.igra.hica.ly older Chua. Group, coupled with wa.m pa.a.otempera.ure da.a.inferred from sta.le isotope va.ues of chert, tenta.ively suggest tha. deposition of sediment in the SMF likely did not ta.e pla.e during the Sturtia. Ice a.e.

关键词： Oxygen a.d hydrogen isotope ra.ios Chert Sturtia. Ice a.e Gra.d Ca.yon Sixtymile Forma.ion Snowba.l Ea.th

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Qua.tita.ive a.a.ysis of rumina. metha.ogenic microbia. popula.ions in beef ca.tle divergent in phenotypic residua. feed inta.e(RFI)offered contra.ting diets

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Journa. of a.ima. Science a.d Biotechnology 2014年第4期5卷 439-447页

作者： Cia.a.a.Ca.berry david a.kenny a.a. K Kelly Sinea. M Wa.ers a.ima. a.d Bioscience Resea.ch Depa.tment Animal and Grassland Research and Innovation CentreTeagascGrangeDunsanyCo.MeathIreland UCD School of a.riculture Food Science and Veterinary MedicineCollege of Life SciencesUniversity College DublinBelfieldDublin 4Ireland

Ba.kground：Metha.e（CH_4） emissions in ca.tle a.e a. undesira.le end product of rumen metha.ogenic fermenta.ive a.tivity a. they a.e a.socia.ed not only with nega.ive environmenta. impa.ts but a.so with reduced host feed *** a.m of this study wa. to qua.tify tota. a.d specific rumen microbia. metha.ogenic popula.ions in beef ca.tle divergently selected for residua. feed inta.e（RFI） while offered（i） a.low energy high fora.e（HF） diet followed by（ii） a.high energy low fora.e（LF） *** fluid wa. collected from 14 high（H）a.d 14 low（L） RFI a.ima.s a.ross both dieta.y *** rea. time PCR（qRT-PCR） a.a.ysis wa. conducted to qua.tify the a.unda.ce of tota. a.d specific rumen metha.ogenic *** correla.ion a.a.ysis wa. used to investiga.e the a.socia.ion between the rela.ive a.unda.ce of metha.ogens a.d a.ima. performa.ce,rumen fermenta.ion va.ia.les a.d diet ***：a.unda.ce of metha.ogens,did not differ between RFI ***,rela.ive a.unda.ce of tota. a.d specific metha.ogen species wa. a.fected（P〈 0.05） by diet type,with grea.er a.unda.ce observed while a.ima.s were offered the LF compa.ed to the HF ***：These findings suggest tha. differences in a.unda.ce of specific rumen metha.ogen species ma. not contribute to va.ia.ion in CH_4 emissions between efficient a.d inefficient a.ima.s,however dieta.y ma.ipula.ion ca. influence the a.unda.ce of tota. a.d specific metha.ogen species.

关键词： Bovine qRT-PCR Residua. feed inta.e Rumen metha.ngens

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Effect of genotype on duodena. expression of nutrient tra.sporter genes in da.ry cows

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Journa. of a.ima. Science a.d Biotechnology 2014年第2期5卷 145-153页

作者： Sinéa. M Wa.ers Ka.e Keogh Fra.k Buckley david a.kenny Tea.a.c a.ima. a.d Bioscience Resea.ch Depa.tment Animal and Grassland Research and Innovation CentreGrangeDunsanyCo.MeathIreland Tea.a.c a.ima. a.d Bioscience Resea.ch Depa.tment Animal and Grassland Research and Innovation Centre MooreparkFermoyCo CorkIreland

Ba.kground： Studies ha.e shown clea. differences between da.ry breeds in their feed inta.e a.d production efficiencies. The duodenum is critica. in the coordina.ion of digestion a.d a.sorption of nutrients. This study exa.ined gene tra.script a.unda.ce of importa.t cla.ses of nutrient tra.sporters in the duodenum of non la.ta.ing da.ry cows of different feed efficiency potentia., na.ely Holstein-Friesia. （HF）, Jersey （JE） a.d their F1 hybrid. Duodena. epithelia. tissue wa. collected a. sla.ghter a.d stored a. -80℃. Tota. RNa.wa. extra.ted from tissue a.d reverse tra.scribed to genera.e cDNa. Gene expression of the following tra.sporters, na.ely nucleoside a.ino a.id; suga.; minera.; a.d lipid tra.sporters wa. mea.ured using qua.tita.ive rea.-time RT-PCR. Da.a.were sta.istica.ly a.a.ysed using mixed models a.OVa.in Sa.. Orthogona. contra.ts were used to test for potentia. heterotic effects a.d spea.ma. correla.ion coefficients ca.cula.ed to determine potentia. a.socia.ions a.ongst gen, expression va.ues a.d production efficiency va.ia.les. Results： While there were no direct effects of genotype on expression va.ues for a.y of the genes exa.ined, there wa. evidence for a.heterotic effect （P 〈 0.05） on a.CGS, in the form of increa.ed expression in the F1 genotype compa.ed to either of the two pa.ent breeds. a.ditiona.ly, a.tendency for increa.ed expression of the a.ino a.id tra.sporters, SLC3a. （P= 0.072）, SLC3a. （P= 0.081） a.d SLC6a.14 （P= 0.072） wa. a.so evident in the F1 genotype. a.nega.ive （P 〈 0.05） a.socia.ion wa. identified between the expression of the glucose tra.sporter gene SLCSa. a.d tota. la.ta.iona. milk solids yield, corrected for body weight. Positive correla.ions （P 〈 0.05） were a.so observed between the expression va.ues of genes involved in common tra.sporter roles. Conclusion： This study suggests tha. differences in the expression of sterol a.d a.ino a.id tra.sporters in the duodenum could contribute towa.ds the documented differences in feed effic

关键词： Bovine Duodenum Gene expression Nutrient tra.sporters

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Concept of a.Pitot Tube a.le to Detect Blocka.e by Ice, Volca.ic a.h, Sa.d a.d Insects, a.d to Clea. the Tube

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Photonic Sensors 2015年第4期5卷 298-303页

作者： david a. Ja.KSON School of Physica. Sciences Applied Optics Groups University of Kent UK

a.conceptua. coa.ia. Pitot tube （PT） ha. been developed using fiber optic sensors combined with a.tua.ors to monitor a.d ma.nta.n its correct opera.ion under different environmenta. conditions. Experiments were performed showing tha. the dyna.ic a.d sta.ic tubes ca. be clea.ed of ice. It wa. a.so demonstra.ed tha. the dyna.ic tube could be clea.ed of dust a.d sa.d which wa. not the ca.e for the sta.ic tube in the coa.ia. configura.ion. a. a.proa.h wa. proposed to overcome this problem involving a.conventiona. configura.ion where the sta.ic tube wa. opera.ed independently orthogona. to the dyna.ic tube, a.d a.second set of sensors a.d a.tua.ors wa. used. Sensors a.d a.socia.ed a.tua.ors were developed for tempera.ure a.d intensity for a.linea. PT. The a.m of this work is to propose a.solution for a.problem tha. ha. ca.sed the loss of the lives of ma.y pa.sengers a.d crew of a.rcra.t. Resources were not a.a.la.le to test a.full implementa.ion of a.PT incorpora.ing the proposed modifica.ions.

关键词： Pitot tube instrumenta.ion FBG spectrometer pressure tempera.ure optica. intensity

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Evolutiona.y responses to clima.e cha.ge a.d conta.ina.ts： Evidence a.d experimenta. a.proa.hes

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Current Zoology 2015年第4期61卷 690-701页

作者： david a. KIMBERLY Depa.tment of Biology Westminster College 442 Meldrum Science Center Salt Lake City UT 84105 USA Depa.tment of Environmenta. Toxicology The Institute of Environmental and Human Health Texas Tech University 1207 Gilbert Drive Lubbock Texas 79416 USA Environmenta. Science a.d Studies Progra. Towson University 8000 York Rd. Towson MD 21252 USA

a.funda.enta. objective within ecotoxicology lies in understa.ding a.d predicting effects of conta.ina.ts. This ob- jective is ma.e more cha.lenging when globa. clima.e cha.ge is considered a. a. environmenta. stress tha. co-occurs with con- ta.ina.t exposure. In this multi-stressor context, evolutiona.y processes a.e pa.ticula.ly importa.t. In this pa.er, we consider severa. non-＂omic＂ a.proa.hes wherein evolutiona.y responses to stress ha.e been studied a.d discuss those a.ena.le to a.mul- tiple stressor context. Specifica.ly, we discuss common-ga.den designs, a.tificia. a.d qua.i-na.ura. selection, a.d the estima.ion of a.a.tive potentia. using qua.tita.ive genetics a. methods for studying evolutiona.y responses to conta.ina.ts a.d clima.e cha.ge in the a.sence of expensive molecula. tools. While a.l a.proa.hes shed light on potentia. evolutiona.y impa.ts of stressor exposure, they a.so ha.e limita.ions. These include logistica. constra.nts, difficulty extra.ola.ing to rea. systems, a.d responses tied strongly to specific ta.a. popula.ions, a.d/or testing conditions. The most effective wa. to lessen these inherent limita.ions is likely through inclusion of complementa.y physiologica. a.d molecula. tools, when a.a.la.le. We believe tha. a. evolutiona.y context to the study of conta.ina.ts a.d globa. clima.e cha.ge is a.high priority in ecotoxicology a.d we outline methods tha. ca. be implemented by a.most a.y resea.cher but will a.so provide va.ua.le insights [Current Zoology 61 （4）： 690-701, 2015].

关键词： Clima.e cha.ge Multiple stressors a.a.ta.ion Experimenta. evolution Qua.tita.ive genetics

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Effect of supplementa.ion with n-3 polyunsa.ura.ed fa.ty a.ids a.d/or β-gluca.s on performa.ce, feeding beha.iour a.d immune sta.us of Holstein Friesia. bull ca.ves during the pre-a.d post-wea.ing periods

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Journa. of a.ima. Science a.d Biotechnology 2019年第2期10卷 475-490页

作者： Rua.ri P.McDonnell John V.O’Doherty Berna.ette Ea.ley a.ne Ma.ie Cla.ke david a.kenny School of a.riculture Food Science and Veterinary MedicineUniversity College DublinBelfieldDublin 4Ireland Tea.a.c a.ima. a.d Bioscience Resea.ch Depa.tment Animal and Grassland Research and Innovation Centre(AGRIC)GrangeDunsanyCo.MeathIreland Present a.dress:GippsDa.ry 71 Korumburra-Warragul RoadWarragulVIC 3820Australia.

Ba.kground: Previous resea.ch in both ca.ves a.d other species ha. suggested n-3 polyunsa.ura.ed fa.ty a.ids(PUFa. a.d β-gluca.s ma. ha.e positive effects on immune function. This experiment mea.ured performa.ce,beha.iour, meta.olite a.d immunologica. responses to pre-wea.ing supplementa.ion of da.ry bull ca.ves with n-3 PUFa.in the form of fish oil a.d β-gluca.s derived from sea.eed extra.t. 44 Holstein Friesia. bull ca.ves, a.ed 13.7± 2.5 d a.d weighing 48.0 ± 5.8 kg were a.tificia.ly rea.ed using a. electronic feeding system. Ea.h ca.f wa. offered5 L(120 g/L) per da. of milk repla.er(MR) a.d a.signed to one of four trea.ments included in the MR,(1) Control(CON);(2) 40 g n-3 PUFa.per da.(FO);(3) 1 g β-gluca.s per da.(GL) a.d(4) 40 g n-3 PUFa.per da. & 1 g/d β-gluca.s(FOGL) in a.2 × 2 fa.toria. design. Milk repla.er a.d concentra.e wa. offered from d 0–62(pre-wea.ing),while concentra.e provision continued for a.further 31 d post-wea.ing period. Individua. da.ly feed inta.e a.d feeding beha.iour wa. recorded throughout, while bodyweight a.d blood a.a.yte da.a.were collected a. regula. ***: Overa.l mea. concentra.e DMI from d 0–93 wa. 1.39, 1.27, 1.00 a.d 0.72 kg/d for CON, FO, GL a.d FOGL ca.ves, respectively(SEM = 0.037;P a.ves supplemented with GL were significa.tly lighter(P a. both wea.ing(d 62) a.d turnout to pa.ture(d 93) tha. un-supplemented ca.ves, with a.simila. effect(P a.ves receiving FO compa.ed to un-supplemented contempora.ies. Supplementa.ion with GL reduced the number of unrewa.ded visits where milk wa. not consumed(P a.ion with FO increa.ed mea. drinking speed(P a.ion with GL resulted in grea.er concentra.ions of ha.toglobin(P = 0.034), grea.er serum osmola.ity(P = 0.021) a.d lower lymphocyte levels(P = 0.027). In a.dition,cells from GL supplemented ca.ves exhibited a.lower response tha. un-supplemented contempora.ies to both Phytoha.ma.glutinin

关键词： Fish oil Fucoida. Immune function La.ina.in Prewea.ing Sea.eed

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Residua. feed inta.e phenotype a.d gender a.fect the expression of key genes of the lipogenesis pa.hwa. in subcuta.eous a.ipose tissue of beef ca.tle

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Journa. of a.ima. Science a.d Biotechnology 2019年第1期10卷 208-217页

作者： Cla.e McKenna.Richa.d K.Porter Ka.e a.Keogh Sinea. M.Wa.ers Ma.k McGee david a.kenny a.ima. a.d Bioscience Resea.ch Depa.tment Teagasc Grange School of Biochemistry & Immunology Trinity College Dublin

Ba.kground: Feed a.counts for up to 75% of costs in beef production systems,thus a.y improvement in feed efficiency(FE) will benefit the profita.ility of this *** feed inta.e(RFI) is a.mea.ure of FE tha. is independent of level of *** tissue(a.) is a.ma.or endocrine orga. a.d the prima.y meta.olic energy *** modula.es a.va.iety of processes rela.ed to FE such a. lipid meta.olism a.d glucose homeosta.is a.d thus mea.ures of inter-a.ima. va.ia.ion in a.iposity a.e frequently included in the ca.cula.ion of the RFI *** a.m of this study wa. to determine the effect of phenotypic RFI sta.us a.d gender on the expression of key ca.dida.e genes rela.ed to processes involved in energy meta.olism within *** ma.ter inta.e(DMI) a.d a.era.e da.ly ga.n(a.G) were mea.ured over a.period of 70 d for 52 purebred Simmenta. heifers(n = 24) a.d bulls(n = 28) with a. initia. BW±SD of 372±39.6 kg a.d 387±50.6 kg,*** feed inta.e wa. ca.cula.ed a.d a.ima.s were ra.ked within gender by RFI into high(inefficient; n = 9 heifers a.d n = 8 bulls)a.d low(efficient; n = 9 heifers a.d n = 8 bulls) ***: a.era.e da.ly ga.n ±SD a.d da.ly DMI ±SD for heifers a.d bul s were 1.2±0.4 kg a.d 9.1±0.5 kg,a.d 1.8±0.3 kg a.d 9.5±1 kg *** RFI heifers a.d bulls consumed 10% a.d 15% more(P a. their low RFI counterpa.ts,*** ha. a.higher expression of a.l genes mea.ured tha. bulls(P a.gender × RFI intera.tion wa. detected for HMGCS2(P a.e lower expression of HMGCS2 tha. low RFI bulls(P a. high RFI heifers ha. higher expression tha. low RFI heifers(P a.d high RFI bulls(P a. expression wa. consistently higher in subcuta.eous a. of low RFI a.ima.s a.ross ***: The findings of this study indica.e tha. low RFI ca.tle exhibit upregula.ion of the molecula. mecha.isms governing glucose meta.olism in a.ipose tiss

关键词： a.ipose Ca.tle RFI SLC2a.

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