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The evaluation of next-generation probiotics on broiler growth performance,gut morphology,gut microbiome,nutrient digestibility,in addition to enzyme production of Bacillus *** vitro

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Animal Nutrition 2024年第3期18卷 133-144页

作者： Jacoba I.Bromfield Shahram Niknafs Xiaojing Chen Juhani von Hellens darwin horyanto Baode Sun Lei Yu Viet Hai Tran Marta Navarro Eugeni Roura Bioproton Pty Ltd Acacia RidgeBrisbaneQueenslandAustralia Centre for Nutrition and Food Sciences Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandBrisbaneQueenslandAustralia Centre for Animal Science Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandBrisbaneQueenslandAustralia

Considerable research has been conducted into the efficacy of individual probiotics in broiler production,however information on the most effective combinations of synergistic Bacillus probiotic is *** study investigated the impact of different Bacillus strain combinations in broiler chickens,as well as in vitro enzyme *** experiment one,a total of 576 Ross 308 broilers at 1 d old were grown for 21 d across 6 treatments of maize-soybean diets(n=12 pens per treatment)to compare three different strain combinations(formulation 1[F1]:3 strains Bacillus amyloliquefaciens;F2:Bacillus coagulans and 2 strains ***;F3:***,Bacillus licheniformis and 2 strains ***;F5:Bacillus subtilis,*** and 2 strains ***),positive control(PC),and a negative control antibiotic treatment group(NC).In Exp.2,a total of 360 one-day-old ROSS308 broilers were used to test five treatments(n=9)including PC,NC,F1 and F5(selected from Exp.1),and F4(Bacillus pumilis and 2 strains ***)in a maize-soybean *** F1 demonstrated a significant improvement in feed conversion ratio(FCR)compared to F2 at d 14(1.49 vs 2.10;P=0.038)and the body weight(BW)at d 21(847.0 g vs 787.4 g)compared to other combinations(P=0.027).The FCR at d 21 tended to be lower in birds fed F1(1.46 vs 1.66)compared to the control(P=0.068).Probiotic treatments had significantly improved nutrient digestibility compared to the PC and ***,probiotic treatments supported the growth of Streptococcus,a common commensal genus and reduced the abundance of genera that correlated with low weight gain such as *** two revealed that F4 improved FCR(P<0.001)and BW at 28 d(P=0.014).In vitro testing showed a high production of protease and amylase by ***,the addition of Bacillus probiotics,particularly containing *** strains and Bacillus pumilus,into the diet of broiler chickens improves

关键词： Broiler Probiotic Bacillus Gut health Microbial profile

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Anaerobic co-digestion of rice straw and digested swine manure with different total solid concentration for methane production

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International Journal of Agricultural and Biological Engineering 2014年第6期7卷 79-90页

作者： darwin Jay J.Cheng Zhimin Liu Jorge Gontupil O-Seob Kwon Department of Agricultural Engineering Syiah Kuala UniversityBanda AcehIndonesia Department of Biological and Agricultural Engineering North Carolina State UniversityRaleighNC 27695USA Department of Environmental Science and Engineering Inje UniversityGimhaeGyeongnam 621-749Korea

This study aimed to investigate potential methane production through anaerobic co-digestion of rice straw and digested swine manure with different total *** research was carried out in bench scale with utilizing batch *** evaluate the stability of anaerobic co-digestion process,the experiment was run in *** anaerobic co-digestion process was operated in 500 mL batch digesters under constant agitation speed and *** agitation speed was maintained at 270 r/*** of the batch system was set and maintained at 35℃.Digested swine manure utilized in this experiment was obtained from semi-continuous digesters run at steady state condition,with 25 days of hydraulic retention time under mesophilic *** straw(RS)generated the highest methane production at 3% total solids(TS)which was around(1814±47.43)mL,where in this concentration,it had C:N ratio at 10.6:*** straw obtained the highest methane yield at 3% TS,which was around(141.4±3.70)mL CH_(4)/g volatile solids(VS)*** straw also had the highest chemical oxygen demand(COD)removal and VS reduction at 3% TS which were around(52.97%±1.46%)and(61.81%±1.04%),respectively.

关键词： anaerobic co-digestion rice straw digested swine manure methane production

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Tuberculous pericarditis-a silent and challenging disease:A case report

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World Journal of Clinical Cases 2022年第6期10卷 1869-1875页

作者： Oscar David Lucero Marlon Mauricio Bustos darwin Jhoan Ariza Rodriguez Juan Camilo Perez Department of Internal Medicine Hospital Universitario San IgnacioBogota 110231Colombia Internal Medicine Resident Pontificia Universidad JaverianaBogota 110231Colombia

BACKGROUND Tuberculous pericarditis(TP)remains a challenge for endemic *** developing countries,one to two percent of patients with pulmonary tuberculosis develops *** SUMMARY A 49-year-old woman presented with dyspnea,chest pain and dry *** physical examination,veiled heart sounds were *** electrocardiogram showed low-voltage complexes and the transthoracic echocardiography revealed a large and free-looking pericardial *** patient was taken for an open *** pericardial fluid revealed high levels of adenosine deaminase and Ziehl-Neelsen stain showed acid-fast *** chain reaction study for Mycobacterium tuberculosis in pericardial fluid was *** patient received tetra conjugate management with adequate clinical response after the first week of treatment and resolution of fever and chest *** In cases of TP,obtaining pericardial fluid and/or pericardial biopsy is the most efficient strategy to confirm the *** diagnosis of this entity will allow physicians to initiate timely treatment,avoid complications and improve the patient's clinical outcome,so we consider the description of this case pertinent and its review in the literature.

关键词： Tuberculosis Pericardial disease Tuberculous pericarditis Pericardial effusion Mycobacterium tuberculosis Case report

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Comparative study on production,purification of penicillin by Penicillium chrysogenum isolated from soil and citrus samples

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Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 15-19页

作者： S Anto Jeya Dayalan Pramod darwin Prakash S Department of Biotechnology Udaya School of Engineering

Objective:To explore various unexplored locations where Penicillium *** be available and study the production of penicillin from the isolated Penicillium *** different media with altered carbohydrate ***:The collected soil samples were screened for the isolation of Penicillium chrysogenum(***) by soil dilution *** isolated Penicillium species were further grown in different production media with changes in the carbohydrate *** extracted penicillin from various isolates was analyzed by HPLC for the efficacy of the *** the products were screened with various bacterial species including methicillin resistant Staphylococcus aureus(MRSA).And the work was extended to find the possible action on MRSA,along with characterization using other ***:From the various soil and citrus samples used for analysis,only the soil sample from Government General Hospital of Bangalore,India,and Sanjay Gandhi Hospital,Bangalore,India,showed some potential growth of the desired fungi *** production media showed varied range of growth of *** production of penicillin was obtained in maltose which proved maximum zone of inhibition during *** of penicillin on pathogens,like wild Escherichia coli strain,Klebsiella spp.,and MRSA,gave quite interesting results such as no activity on the later strain as it is *** data provided the analytical and confirmation details of the penicillin ***,the penicillin produced from the soil sample of Government General Hospital had the high milli absorbance unit of 441.5 mAu compared with that of the penicillin produced from Sanjay Gandhi Hospital sample,8S.S2 ***,there was a considerable change in quantity of the penicillin produced from both the ***: The Penicillium *** be possibly rich in hospital contaminants and its *** research focuses on various unexplored sources o

关键词： Penicillium chrysogenum Soil Citrus Penicillin Bioefficacy Antibiotic activity Penicillium spp. Production Soil dilution plate Staphy lococcus aureus Characterization Production media Zone of inhibition Carbohydrate source Isolation Efficacy Pathogen

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The Sandfish's Skin:Morphology,Chemistry and Reconstruction

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Journal of Bionic Engineering 2007年第1期4卷 1-9页

作者： Werner Baumgartner Friederike Saxe Agnes Weth David Hajas darwin Sigumonrong Jens Emmerlich Martin Singheiser Wolfgang Bhme Jochen M. Schneider Department of Cellular Neurobionics RWTH-Aachen Kopernikusstr. 16 52056 Aachen Germany Material Chemistry RWTH-Aachen Kopernikusstr. 16 52056 Aachen Germany Institute of Zoology RWTH-Aachen Kopernikusstr. 16 52056 Aachen Germany Zoologisches Forschungsmuseum Alexander Koenig (ZFMK) Adenauerallee 160 53113 Bonn Germany

The sandfish is a lizard having the remarkable ability to move in desert sand in a swimming-like fashion. The most outstanding adaptations to this mode of life are the low friction behaviour and the extensive abrasion resistance of the sandfish skin against sand, outperforming even steel. We investigated the topography, the composition and the mechanical properties of sandfish scales. These consist of glycosylated keratins with high amount of sulphur but no hard inorganic material, such as silicates or lime. Remarkably, atomic force microscopy shows an almost complete absence of attractive forces between the scale surface and a silicon tip, suggesting that this is responsible for the unusual tribological properties. The unusual glycosylation of the keratins was found to be absolutely necessary for the described phenomenon. The scales were dissolved and reconstituted on a polymer surface resulting in properties similar to the original scale. Thus, we provide a pathway towards exploitation of the reconstituted scale material for future engineering applications.

关键词： Sandfish Skink Scincus scincus tribology nanoindentation keratin abrasion

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Robot impedance control and passivity analysis with inner torque and velocity feedback loops

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Control Theory and Technology 2016年第2期14卷 97-112页

作者： Michele FOCCHI Gustavo A. MEDRANO-CERDA Thiago BOAVENTURA Marco FRIGERIO Claudio SEMINI Jonas BUCHLI darwin G. CALDWELL Department of Advanced Robotics Istituto Italiano di Tecnologia (liT) via Morego 30 16163 Genova Italy Agile & Dexterous Robotics Lab ETH Zurich Tannenstrasse 3 8092 Zurich Switzerland Received 21 January 2015 revised 10 December 2015 accepted 10 December 2015

Impedance control is a well-established technique to control interaction forces in robotics. However, real implementations of impedance control with an inner loop may suffer from several limitations. In particular, the viable range of stable stiffness and damping values can be strongly affected by the bandwidth of the inner control loops （e.g., a torque loop） as well as by the filtering and sampling frequency. This paper provides an extensive analysis on how these aspects influence the stability region of impedance parameters as well as the passivity of the system. This will be supported by both simulations and experimental data. Moreover, a methodology for designing joint impedance controllers based on an inner torque loop and a positive velocity feedback loop will be presented. The goal of the velocity feedback is to increase （given the constraints to preserve stability） the bandwidth of the torque loop without the need of a complex controller.

关键词： Impedance control torque control passivity and stability analysis

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Mass Spectrometry-based Quantitative Proteomic Profiling of Human Pancreatic and Hepatic Stellate Cell Lines

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Genomics, Proteomics & Bioinformatics 2013年第2期11卷 105-113页

作者： Joao A.Paulo Vivek Kadiyala Peter A.Banks darwin L.Conwell Hanno Steen Department of Pathology Boston Children's Hospital Proteomics Center at Boston Children's Hospital Center for Pancreatic Disease Division of GastroenterologyHepatology and EndoscopyBrigham and Women's Hospital and Department of MedicineHarvard Medical School

The functions of the liver and the pancreas differ; however, chronic inflammation in both organs is associated with fibrosis. Evidence suggests that fibrosis in both organs is partially regu- lated by organ-specific stellate cells. We explore the proteome of human hepatic stellate cells （hHSC） and human pancreatic stellate cells （hPaSC） using mass spectrometry （MS）-based quanti- tative proteomics to investigate pathophysiologic mechanisms. Proteins were isolated from whole cell lysates of immortalized hHSC and hPaSC. These proteins were tryptically digested, labeled with tandem mass tags （TMT）, fractionated by OFFGEL, and subjected to MS. Proteins significantly different in abundance （P 〈 0.05） were classified via gene ontology （GO） analysis. We identified 1223 proteins and among them, 1222 proteins were quantifiable. Statistical analysis determined that 177 proteins were of higher abundance in hHSC, while 157 were of higher abundance in hPaSC. GO classification revealed that proteins of relatively higher abundance in hHSC were associated with protein production, while those of relatively higher abundance in hPaSC were involved in cell structure. Future studies using the methodologies established herein, but with further upstream fractionation and/or use of enhanced MS instrumentation will allow greater proteome coverage, achieving a comprehensive proteomic analysis of hHSC and hPaSC.

关键词： Fibrosis Proteomics Pancreas Tandem mass tag

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On the use of positive feedback for improved torque control

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Control Theory and Technology 2015年第3期13卷 266-285页

作者： Houman DALLALI Gustavo A.MEDRANO-CERDA Michele FOCCHI Thiago BOAVENTURA Marco FRIGERIO Claudio SEMINI Jonas BUCHLI darwin G.CALDWELL Department of Advanced Robotics Istituto Italiano di Tecnologia(IIT) Agile and Dexterous Robotics Lab Institute of Robotics and Intelligent SystemsETH

This paper considers the torque control problem for robots with flexible joints driven by electrical actuators. It is shown that the achievable closed-loop tracking bandwidth using PI torque controllers may be limited due to transmission zeros introduced by the load dynamics. This limitation is overcome by using positive feedback from the load motion in unison with PI torque controllers. The positive feedback is given in terms of load velocity, acceleration and jerk. Stability conditions for designing decentralized PI torque controllers are derived in terms of Routh-Hurwitz criteria. Disturbance rejection properties of the closed system are characterized and an analysis is carried out investigating the use of approximate positive feedback by omitting acceleration and/or jerk signals. The results of this paper are illustrated for a two DoF （degrees of freedom） system. Experimental results for a one DoF system are also included.

关键词： Force-torque control load motion compensation decentralized control

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Tomcat权威指南

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作者： darwin Brittain

来源：南通市图书馆图书评论

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一个在塑胶射出市场营销策略研究—案例研究Tanindo Nobel Plast, Ltd.

一个在塑胶射出市场营销策略研究—案例研究Tanindo Nobel Plast,...

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作者： darwin Tirta Kayuwangsa 北京交通大学

学位级别：硕士

问题应该是一种有效的营销策略Tanindo Nobel Plast, Ltd以增加其市场份额? 目的本研究的目的是调查和分析当前的注塑市场状况以及竞争对手,从调查中使用的知识,要考虑一个更好的营销组合申请Tanindo Nobel Plast, Ltd. 方法笔者...

问题应该是一种有效的营销策略Tanindo Nobel Plast, Ltd以增加其市场份额? 目的本研究的目的是调查和分析当前的注塑市场状况以及竞争对手,从调查中使用的知识,要考虑一个更好的营销组合申请Tanindo Nobel Plast, Ltd. 方法笔者收集采访和问卷调查,以了解公司的市场运作和营销组合的客户的反馈的主要数据。笔者还收集了可靠的辅助数据,与五力框架的指引学习的营销情况。根据选定的理论和收集到的数据进行分析。相应的分析,笔者提出了有关建议。目标受众目标受众是营销总监,业务经理和Tanindo Nobel Plast, Ltd客户。笔者希望纸张的结果可能导致市场上的表现为他们带来一些好处。Tanindo Nobel Plast, Ltd可以使用的研究结果,设计一个有效的战略。笔者认为,这项研究可以提供一般性的指导,并造福类似领域的营销,并帮助他们学习的竞争局面。结论从结果,笔者认为,虽然Tanindo Nobel Plast, Ltd有一个良好的产品质量,市场情况是不是真的好Tanindo Nobel Plast, Ltd。因此,提高市场占有率,以及支持其领先地位,Tanindo Nobel Plast, Ltd应保持现有的竞争优势,提高营销运作的弊端,从客户的反馈发现的营销组合。笔者通过分析和建议,希望调查结果可以受益Tanindo Nobel Plast, Ltd,并为他们带来设计一个有效的营销策略的线索。

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