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Nrf2 and Snail-1 in the prevention of experimental liver fibrosis by caffeine

World Journal of Gastroenterology 2013年第47期19卷 9020-9033页

作者： daniela Gordillo-Bastidas Edén Oceguera-Contreras Adriana Salazar-montes Jaime González-Cuevas Luis Daniel Hernández-Ortega Juan Armendáriz-Borunda nstitute of molecular Biology in medicine and Gene Therapy/CUCS University of GuadalajaraGuadalajaraJalisco 44100México

AIm:To determine the molecular mechanisms involved in experimental hepatic fibrosis prevention by caffeine(CFA).mETHODS:Liver fibrosis was induced in Wistar rats by intraperitoneal thioacetamide or bile duct ligation and they were concomitantly treated with CFA(15 mg/kg per day).Fibrosis and inflammatory cell infiltrate were evaluated and classified by Knodell *** infiltrate was quantified by immunohistochemistry(anti-CD11b).Gene expression was analyzed by quantitative reverse transcription-polymerase chain reaction for collagenⅠ?(Col-1),connective tissue growth factor(CTGF),transforming growth factorβ1(TGF-β1),tumor necrosis factor alpha(TNF-α),interleukin-1(IL-1),IL-6,superoxide dismutase(SOD)and catalase(CAT).Activation of Nrf2 and Snail-1 was analyzed by ***-αexpression was proved by enzyme-linked immunosorbant assay,CAT activity was performed by ***:CFA treatment diminished fibrosis index in treated *** Knodell index showed both lower fibrosis and *** of profibrogenic genes CTGF,Col-1 and TGF-β1 and proinflammatory genes TNF-α,IL-6 and IL-1 was substantially diminished with CFA treatment with less CD11b positive *** lower values of transcriptional factor Snail-1 were detected in CFA treated rats compared with cirrhotic rats without treatment;in contrast Nrf2was increased in the presence of *** of SOD and CAT was greater in animals treated with CFA showing a strong correlation between mRNA expression and enzyme ***:Our results suggest that CFA inhibits the transcriptional factor Snail-1,down-regulating profibrogenic genes,and activates Nrf2 inducing antioxidant enzymes system,preventing inflammation and fibrosis.

关键词： Liver fibrosis Caffeine Thioacetamide Bile duct ligation Profibrogenic genes Proinflammatory cytokines Antioxidant enzymes

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LC-mS Phytochemical Profiles of Phenolic Compounds and Antimicrobial, Antioxidant, and Antiplasmodial Activities of Ethanol Extracts of Pycnostachys erici-rosenii *** and Leucas martinicensis (Jack.) *** (Lamiaceae)

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Advances in Biological Chemistry 2023年第6期13卷 209-227页

作者： Uwikunda Serondo Héritier Nizigiyimana Libérata Pius Tshimankinda mpiana Nyamangyoku Ishibwela Obedi Ngezahayo Jérémie Dan Cristian Vodnar daniela Hanganu Ioana matei Sanda Andrei Department of Chemistry Faculty of Sciences Institut Supérieur Pédagogique de Bukavu Bukavu Democratic Republic of Congo Centre de Recherche en Sciences Naturelles et de l’Environnement (CRSNE) Faculté des Sciences Université du Burundi Bujumbura Burundi Department of Preclinical Sciences Faculty of Veterinary Medicine University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Cluj-Napoca Romania Ecole Doctorale de l’Université du Burundi Bujumbura Burundi Department of Chemistry Faculty of Sciences Université de Kinshasa Kinshasa Democratic Republic of Congo Faculty of Agriculture Université Catholique de Bukavu Bukavu Democratic Republic of Congo Department of Food Science Faculty of Food Science and Technology University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Cluj-Napoca Romania Department of Pharmacognosy Faculty of Pharmacy University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca Romania Department of microbiology Mycology and Immunology Faculty of Veterinary Medicine University of Agricultural Science and Veterinary Medicine Cluj-Napoca Romania

This study has been conducted to analyze the chemical composition and antimicrobial, antioxidant and antiplasmodial properties of ethanol extracts of Pycnostachys erici-rosenii *** and Leucas martinicensis (Jack.) *** that are not yet well known. The HPLC-DAD-mS-ESI+ method was used to investigate the chemical profile, the disc diffusion and microdilution method for the antimicrobial activities, the DPPH and FRAP assays for antioxidant property and SYBR Green I-based growth inhibition assay on Pf 3D7 (CQ-sensitive) strain for antiplasmodial properties. The results indicated significant amounts of hydroxybenzoic acid, flavonol and hydroxycinnamic acid in the two species. The total polyphenols analysis gave 209.0 ± 16.1 mg GAE/100g DW for P. erici-rosenii and 175.3 ± 1.6 mg GAE/100g DW for L. martinicensis while for total flavonoids analysis 33.4 ± 1.8 mg TE/100g DW was found for P. erici-rosenii and 39.6 ± 0.6 mg TE/100g DW for L. martinicensis. moreover, antimicrobial tests revealed 0.125 mg/mL on E. faecalis ATCC25922 for ethanol extracts P. erici-rosenii and L. martinicensis. The antioxidant assays revealed IC50 56.17 μg/mL and 78.43 μg/mL for DPPH assay, and 112.03 μm TE/100mL and 90.46 μm TE/100mL) for FRAP assay for ethanol extracts of P. erici-rosenii and L. martinicensis respectively. Finally, for Antiplasmodial activity, IC50 was 4.07 μg/mL for P. erici-rosenii and 12.29 μg/mL for L. martinicensis. Therefore, the antimicrobial, the antioxidant, and the antiplasmodial results suggest that the ethanolic extracts of the aerial parts of P. erici-rosenii and L. martinicensis contain compounds with antimicrobial, antioxidant, and antiplasmodial properties, which can be searched in new drug discovery for treatment of infectious diseases.

关键词： Pycnostachys erici-rosenii Leucas martinicensis Phytochemical Profile Antimicrobial Activity Antioxidant Activity Antiplasmodial Activity

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RHAU解旋酶水解ATP解开G-四链体机制的单分子研究

RHAU解旋酶水解ATP解开G-四链体机制的单分子研究

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第十届全国化学生物学学术会议

作者：游慧娟 Simon Lattmann daniela Rhodes 严洁华中科技大学同济药学院南洋理工大学结构生物学研究所新加坡国立大学力学生物学研究所

解旋酶是一类调节核酸的二级结构的马达蛋白。RHAU解旋酶特异性的与G-四链体结合,调节体内如端粒和m RNA等相关序列中的G-四链体结构。目前对于解旋酶如何与G-四链体相互作用并打开G-四链体的机制仍不清楚。我们通过单分子磁镊直接观察... 详细信息

解旋酶是一类调节核酸的二级结构的马达蛋白。RHAU解旋酶特异性的与G-四链体结合,调节体内如端粒和m RNA等相关序列中的G-四链体结构。目前对于解旋酶如何与G-四链体相互作用并打开G-四链体的机制仍不清楚。我们通过单分子磁镊直接观察单个G-四链体分子的折叠和去折叠过程,发现在没有ATP时,解旋酶RHAU同G-四链体的结合将稳定G-四链体。在AmP-PNP,ADP的结合状态下,RHAU解旋酶均表现出稳定G-四链体的效果。而在ATP水解中间状态的下,RHAU的结合不能稳定G-四链体。由以上结果我们提出了RHAU水解ATP解链G-四链体的工作模型。我们的研究也证实了3’-ssDNA,RHAU的N-末端对于RHAU解链G-四链体是必须的。

关键词： G-四链体解旋酶单分子

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When sepsis affects the heart: A case report and literature review

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World Journal of Clinical Cases 2015年第8期3卷 743-750页

作者： Giuseppe Clemente Antonino Tuttolomondo daniela Colomba Rosaria Pecoraro Chiara Renda Vittoriano Della Corte Carlo maida Irene Simonetta Antonio Pinto U.O.C.di medicina Interna e Cardioangiologia University of Palermo

A 59-year-old nursing home patient with Down syndrome was brought to the internal medicine department of our hospital due to fever, cough without expectorate, and dyspnea. A thoracic computed tomography revealed the presence of bilateral basal parenchymal opacities. Her condition deteriorated after admission and troponin reached a peak serum concentration of 16.9 ng/m L. The patient was in cardiogenic shock. In addition to fluid resuscitation, vaso-active amine infusion was administered to achieve hemodynamic stabilization. The differential diagnosis investigated possible pulmonary embolism, myocardial infarction, and myocarditis. Furthermore, a second transthoracic echocardiogram suggested Tako-Tsubo syndrome. This is a septic patient. The purpose of this manuscript is to review studies which formerly examined the possible association between high levels of troponin and mortality to see if it can be considered a positive predictive factor of fatal prognosis as the case of thrombocytopenia, already a positive independent predictive factor of multiple organ failure syndrome, and generally to characterize risk profile in a septic patient.

关键词： Sepsis Shock Hypotension Troponin myocardial dysfunction

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小麦杂交种衡杂102丰产性节水性鉴定及分析

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种子 2012年第12期31卷 59-61页

作者：李慧敏赵明辉张玉兰袁邵华赵凤梧 daniela Benedikova Pavol Hauptvogel Edita Gregova 河北省农林科学院旱作农业研究所河北衡水053000 衡水市农牧局河北衡水053000 植物生产研究中心斯洛伐克皮斯坦尼12292168

通过化学杀雄配制出杂交种衡杂102,表现出较好的高产性、节水性及适应性。水处理单位面积穗数、穗粒数超标优势为5.77%和3.97%,均达0.05显著水平;千粒重、产量超标优势为9.36%和12.21%,达0.01极显著水平。节水处理平均产量482.44 kg/667... 详细信息

通过化学杀雄配制出杂交种衡杂102,表现出较好的高产性、节水性及适应性。水处理单位面积穗数、穗粒数超标优势为5.77%和3.97%,均达0.05显著水平;千粒重、产量超标优势为9.36%和12.21%,达0.01极显著水平。节水处理平均产量482.44 kg/667 m2,比对照增产8.29%,名列30个参试品种第1名。通过杂种优势利用,可以选育出高产、节水的小麦杂交种。

关键词：小麦化学杀雄杂交种丰产节水杂种优势

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Culture Circle as a Teaching Approach in the Education of Teenager Health multipliers on Leprosy Awareness

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Health 2015年第14期7卷 1813-1823页

作者： Estela maria Leite meirelles monteiro Amanda Araújo das merces Amanda Carla Borba de Souza Cavalcanti Ana márcia Tenorio de Souza Cavalcanti Ana Catarina Torres de Lacerda Rosália daniela medeiros da Silva Andrea Rosane Sousa Silva Waldemar Brandao Neto Graduate Program in Nursing Federal University of Pernambuco (UFPE) Recife Brazil Graduate Program in Child and Adolescent Health Centre of Health Sciences Federal University of Pernambuco Recife Brazil Nursing Department at the Federal University of Pernambuco Recife Brazil

Objective: The study aims to evaluate the use of a teaching method proposed by Paulo Freire, Culture Circles, in the education of teenagers multipliers on leprosy awareness. methods: It is an action-research study with a qualitative approach developed in a public school in Pernambuco, Brazil. Five Culture Circles were conducted involving the participation of 26 teenagers. The followings were used as data collection tools: observation, field notes, photography and filming. Results: The educational intervention on health addressed the following topics: 1) Definition and transmission of leprosy;2) Characteristics and diagnosis of leprosy;3) Treatment of leprosy;4) Aesthetics, prejudice and mental health related to leprosy;and 5) Planning of educational activities for teenagers as health multipliers on leprosy awareness. The educational action on health provided this age group to perceive themselves, act as political subjects in the development of Culture Circles, and act as protagonists in the dissemination of knowledge on leprosy. Conclusions: This study highlights that the application of active methodologies, such as Culture Circles, is able to encourage the engagement of young people in community empowerment and bring together health professionals and the school community in an intersectoral work in order to develop action strategies involving the promotion of health in the context of neglected diseases such as leprosy.

关键词： Adolescents School Health Leprosy Awareness Culture Circles Health Promotion

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Antimalarial Activity of an Alkaloid mixture Isolated from the Tender Leaves of the Nectandra membranacea （Laureaceae）

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Journal of Life Sciences 2018年第3期12卷 134-140页

作者： Vanessa Bagnarello-madrigal daniela Rodríguez-Chaves Javier Alpizar-Cordero Alonso Calvo-Vargas maribel Cordero-Villalobos misael Chinchilla-Carmona Idalia Valerio-Campos Ronald Sánchez -Porras Departamento de Química Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Departamento de Investigación Básica Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Sección de Biología Sede Occidente Universidad de Costa Rica San José 10108 Costa Rica

Despite the breadth of knowledge about malaria, its transmission, preventive measures and available treatments and its difficulty in controlling the vector and the rapid spread of resistance to current drugs, there is a stimulus to develop new research looking for active molecules. The phytochemical studies of the active extract confirmed the presence of alkaloids, flavonoids, coumarins, tannins, reducing compounds, anthocyanins, terpenes, triterpenes and steroids. In the present work, it was possible to isolate a mixture of isoquinolinic alkaloids from the tender leaves of Nectandra membranacea （Laureaceae） with antimalarial activity. The mixture composed of （-） - gliazovine and （＋） - aplogliazovine showed an IC50 value of 32.18 μg/mL against the P. berghei NK65 strain. It is important to continue with these studies and their chemical components isolated from N. membranacea that could be used as an alternative the treatment of malaria.

关键词： malaria Nectandra membranacea alkaloids phytochemical screening Laureaceae.

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小麦抗旱新种质衡562性状表现及分析

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种子 2012年第3期31卷 67-68页

作者：李慧敏赵明辉袁邵华赵凤梧王雪征陈淑萍 daniela Benedikova Pavol Hauptvogel 河北省农作物抗旱研究重点实验室河北衡水053000 Plant Production Research Center Piestany12292168Slovakia

连续9年通过对普通小麦基因型石4185(***)与硬粒小麦基因型4184(***)种间杂交后代回交、顶交及辐射处理,选育出节水、高产小麦新种质衡562。近红外技术测定,抗旱指数为1.2。2008～2009年河北省农林科学院旱作农业研究所作水、旱处理,水... 详细信息

连续9年通过对普通小麦基因型石4185(***)与硬粒小麦基因型4184(***)种间杂交后代回交、顶交及辐射处理,选育出节水、高产小麦新种质衡562。近红外技术测定,抗旱指数为1.2。2008～2009年河北省农林科学院旱作农业研究所作水、旱处理,水地产量442.39 kg/667 m2,比对照石4185(400.26 kg/667 m2)增产10.53%;旱地产量366.39kg/667 m2,比对照石4185(334.53 kg/667 m2)增产9.52%。2009～2010年在河北省区域试验节水组预备试验中,平均产量394.97 kg/667 m2,比对照增产9.06%,名列24个参试品种第1名。衡562的育成,为小麦节水、抗旱育种提供了具有应用价值的新种质资源。

关键词：小麦节水高产种质资源性状分析

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Good adherence to mediterranean diet can prevent gastrointestinal symptoms:A survey from Southern Italy

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2016年第4期7卷 564-571页

作者： Francesco Paolo Zito Barbara Polese Letizia Vozzella Antonietta Gala daniela Genovese Viviana Verlezza Fabiana medugno Antonello Santini Luigi Barrea martina Cargiolli Paolo Andreozzi Giovanni Sarnelli Rosario Cuomo Department of Surgery Gastroenterology and EndocrinologyUniversity of Naples "Federico Ⅱ" Department of Pharmacy University of Naples "Federico Ⅱ" IOS s.r.l and COLEmAN S.p.A.

AImTo evaluate how different levels of adherence to a mediterranean diet (mD) correlate with the onset of functional gastrointestinal disorders. mETHODSAs many as 1134 subjects (598 m and 536 F; age range 17-83 years) were prospectively investigated in relation to their dietary habits and the presence of functional gastrointestinal symptoms. Patients with relevant chronic organic disease were excluded from the study. The mediterranean Diet Quality index for children and adolescents (KIDmED) and the Short mediterranean Diet Questionnaire were administered. All subjects were grouped into five categories according to their ages: 17-24 years; 25-34; 35-49; 50-64; above 64. RESULTSOn the basis of the Rome III criteria, our population consisted of 719 (63.4%) individuals who did not meet the criteria for any functional disorder and were classified as controls (CNT), 172 (13.3%) patients meeting criteria for prevalent irritable bowel syndrome (IBS), and 243 (23.3%) meeting criteria for prevalent functional dyspepsia (FD). A significantly lower adherence score in IBS (0.57 ± 0.23, P P P P P P P P P CONCLUSIONLow adherence to mD may trigger functional gastrointestinal symptoms, mainly in younger subjects. moreover, with increasing age, patients tend to adopt dietary regimens closer to mD.

关键词： mediterranean diet Irritable bowel syndrome Dietary regimen Functional gastro-intestinal disorders Functional dyspepsia

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Prediction of genetic value for sweet cherry fruit maturity among environments using a 6K SNP array

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Horticulture Research 2019年第1期6卷 1623-1637页

作者： Craig m.Hardner Ben J.Hayes Satish Kumar Stijn Vanderzande Lichun Cai Julia Piaskowski JoséQuero-Garcia JoséAntonio Campoy Teresa Barreneche daniela Giovannini Alessandro Liverani Gérard Charlot miguel Villamil-Castro Nnadozie Oraguzie Cameron P.Peace University of Queensland Queensland Alliance for Agriculture and Food InnovationBrisbaneQLD 4072Australia The New Zealand Institute for Plant and Food Research Limited Hawke’s Bay Research CentreHastings 4130New Zealand Department of Horticulture Washington State UniversityPullmanWA 99164USA Department of Horticulture Michigan State UniversityEast LansingMI 48824USA UmR 1332 BFP INRAUniversity of Bordeaux33140 Nouvelle-AquitaineFrance Council for Agricultural Research and Economics(CREA) Fruit Unit of ForlìVia la Canapona1 bis47121 Emilia-RomagnaItaly Centre Technique Interprofessionnel des Fruits et Légumes(CTIFL) 751 Chemin de Balandran30127 BellegardeFrance Department of Horticulture Washington State UniversityIrrigated Agriculture Research and Extension Center24106N Bunn RoadProsserWA 99350USA

The timing of fruit maturity is an important trait in sweet cherry production and *** variation for phenology of fruit maturity in sweet cherry appears to be under strong genetic control,but that control might be complicated by phenotypic instability across *** such genotype-by-environment interaction(G×E)is a common phenomenon in crop plants,knowledge about it is lacking for fruit maturity timing and other sweet cherry *** this study,1673 genome-wide SNP markers were used to estimate genomic relationships among 597 weakly pedigree-connected individuals evaluated over two seasons at three locations in Europe and one location in the USA,thus sampling eight‘environments’.The combined dataset enabled a single meta-analysis to investigate the environmental stability of genomic *** disequilibrium among marker loci declined rapidly with physical distance,and ordination of the relationship matrix suggested no strong structure among *** most parsimonious G×E model allowed heterogeneous genetic variance and pairwise covariances among ***-sense genomic heritability was very high(0.60–0.83),as was accuracy of predicted breeding values(>0.62).Average correlation of additive effects among environments was high(0.96)and breeding values were highly correlated across *** indicated that genomic models can be used in cherry to accurately predict date of fruit maturity for untested individuals in new *** G×E for this trait indicated that phenotypes of individuals will be stable across similar *** analyses for other sweet cherry traits,for which multiple years of data are commonly available among breeders and cultivar testers,would be informative for predicting performance of elite selections and cultivars in new environments.

关键词： maturity environments breeding

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