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Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4℃

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Asian Journal of Andrology 2011年第2期13卷 281-286页

作者： Oscar Vera-Munoz Lamia Amirat-Briand Djemil Bencharif Marc Anton Serge Desherces Eric Shmitt Chantal Thorin daniel tainturier Laboratory of Biotechnology and Pathology of Reproduction ONIRIS Nantes-Atlantic College of Veterinary Medicine and Food Science 44307 Nantes France Facultad deCiencias Veterinarias Instituto de Reproduccion Animal Abraham Hemndez Prado Universidad Centra de Venezuela Maracay 2101 Aragua Venezuela Groupe Physico-chimie des Emulsions Laboratoire d＇Etude des Interactions des Molecules Alimentaires Institut National de la Recherche Agronomique 44316 Nantes Cedex 3 France MVTechnologies I＇Aigle 61300 France

An extender has been developed with low-density lipoproteins （LDLs） that eliminates the microbial risks associated with the use of whole egg yolk. The objective of this study was to assess the effects of substituting egg yolk with LDLs for use as an extender in sperm preservation at 4 ℃, as well as on spermatozoa motility, plasma membrane and acrosome integrity, at two different concentrations （80×10^6 and 240× 10^6 sperm per ml） for 8 days and to evaluate glycerol toxicity in both extenders. A total of 12 ejaculates were collected from three bulls. Spermatozoa motility was examined using computer-assisted semen analysis. Plasma membrane integrity was determined using the hypo-osmotic swelling test and acrosome integrity with the fluorescein isothiocyanate-Pisum sativum agglutinin test. The semen was subsequently divided into four aliquots and diluted with Tris-egg yolk-glycerol （TEG）, Tris-egg yolk without glycerol （TE）, LDL with glycerol （LDL＋） and LDL without glycerol （LDL-）, at 80×10^6 and 240 ×10^6sperm per ml. This study showed that the LDL＋ and LDL- extenders were more effective at preserving spermatozoa motility, plasma membrane integrity and acrosome integrity than TEG and TE （P〈0.05） during 8 days of incubation. After 3 days of incubation, a toxicity of glycerol was observed in TEG, whereas no significant difference was observed between LDL＋ and LDL-. We can therefore conclude that the LDL extender can be used to refrigerate semen at 4 ~C instead of TEG and TE at 80×10^6and 240×10^6 sperm per ml for elite bulls. This finding can be used to define a policy for the storage of high-quality bull semen.

关键词： bull semen glycerol low-density lipoproteins membrane integrity motility refrigeration

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