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Progress in Natural Science:Materials International 2006年第11期16卷 1231-1232页

作者： N.V.CHANDRASEKHARAN daniel l.SIMMONS Department of Chemistry and Biochemistry Brigham Young UniversityProvoUtah 84602USA

Selecting bacteria transformed with recombinant plasmid is a laborious step in gene cloning experiments. This selection process is even more tedious when large numbers of clones need to be screened. We describe here modifications to the ultra fast plasmid preparation method described previously by law and Crickmore. The modified method is coupled to an efficient PCR step to rapidly determine orientation of the inserts. Compared to traditional methods of analysis requiring growth of overnight cultures, plasmid isolation and restriction enzyme digestion to determine orientation this procedure allows for the analysis and storage of a large number of recombinants within a few hours.

关键词： plasmid preparation polymerized chain reaction

links between donor macrosteatosis,interleukin-33 and complement after liver transplantation

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World Journal of Transplantation 2020年第5期10卷 117-128页

作者： Kelley Núñez Mohammad Hamed daniel Fort David Bruce Paul Thevenot Ari cohen Institute of Translational Research Ochsner Clinic FoundationNew OrleansLA 70121United States Center for Outcomes and Health Services Research Research AdministrationOchsner Clinic FoundationNew OrleansLA 70121United States Multi-Organ Transplant Program Ochsner Clinic FoundationNew OrleansLA 70121United States

BACKGROUND As prevalence of nonalcoholic fatty liver disease increases in the population,livers with steatosis will continue to infiltrate the donor *** utilization of these extended criteria grafts is paramount given the increased risk associated with their use in *** factors that can predict liver dysfunction immediately after transplantation with macrosteatotic grafts are *** To understand the relationship between interleukin-33(Il-33)and complement in recipients immediately following liver reperfusion as a marker of liver *** Cohort consisted of patients who received a liver transplant from September 2016–September 2019 at our *** variables were retrospectively extracted from the electronic medical ***-table donor biopsies were obtained with donor steatosis percentage retrospectively determined by a boardcertified *** samples were available immediately following liver *** of plasma Il-33 and complement proteins,C3a and C5a,were determined by enzyme-linked immunosorbent *** mRNA expression,RNA was extracted from donor biopsies and used against a 780 gene *** Cohort consisted of 99 donor and *** median age was 45 years and 55%*** had a median age of 59 years with 62%*** main etiologies were alcoholic hepatitis,nonalcoholic steatohepatitis,and hepatocellular *** MElD-Na at transplant was *** were grouped based on moderate macrosteatosis(≥30%).Recipients implanted with moderate macrosteatotic grafts had significantly higher peak alanine aminotransferase/aspartate aminotransferase(Ply allograft dysfunction(60%compared to 18%).Circulating Il-33 levels were significantly elevated in recipients of≥30%macrosteatotic grafts(Ple levels of circulating Il-33 immediately following reperfusion had significantly highe

关键词： liver transplantation Interleukin-33 Donor macrosteatosis Complement Early allograft dysfunction Reperfusion

Role of seed size, phenology, oogenesis and host distribution in the specificity and genetic structure of seed weevils (Curculio spp.) in mixed forests

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Integrative Zoology 2018年第3期13卷 267-279页

作者： Harold ARIAS-lEClAIRE Raúl BONAl daniel GARCÍA-lÓPEZ Josep Maria ESPElTA CREAF Centre for Ecological Research and Forestry ApplicationsCerdanyola del Vallès08193Spain Exact and Natural Sciences School State Distance University of Costa RicaUNEDMercedes de Montes de OcaSan JoséCosta Rica Forest Research Group INDEHESAUniversity of ExtremaduraPlasenciaSpain DITEG Research Group University of Castilla-La ManchaToledoSpain Zoology Department Autonomous University of BarcelonaCerdanyola del VallèsSpain

Synchrony between seed growth and oogenesis is suggested to largely shape trophic breadth of seed-feeding insects and ultimately to contribute to their co-existence by means of resource partitioning or in the time when infestation *** we investigated:(i)the role of seed phenology and sexual maturation of females in the host specificity of seed-feeding weevils(Curculio spp.)predating in hazel and oak mixed forests;and(ii)the consequences that trophic breadth and host distribution have in the genetic structure of the weevil *** analyses were used to establish unequivocally host specificity and to determine the population genetic *** identified 4 species with different specificity,namely Curculio nucum females matured earlier and infested a unique host(hazelnuts,Corylus avellana)while 3 species(Curculio venosus,Curculio glandium and Curculio elephas)predated upon the acorns of the 2 oaks(Quercus ilex and Quercus pubescens).The high specificity of *** coupled with a more discontinuous distribution of hazel trees resulted in a significant genetic structure among *** addition,the presence of an excess of local rare haplotypes indicated that *** populations went through genetic expansion after recent ***,these effects were not observed in the more generalist Curculio glandium predating upon ***,co-existence of weevil species in this multi-host-parasite system is influenced by both resource and time *** what extent the restriction in gene flow among *** populations may have negative consequences for their persistence in a time of increasing disturbances(*** in Mediterranean areas)deserves further research.

关键词： Corylus avellana Curculio spp. genetic structure Quercus spp. trophic breadth

Identification of KASP markers and putative genes for pre-harvest sprouting resistance in common wheat(Triticum aestivum l.)

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The Crop Journal 2023年第2期11卷 549-557页

作者： Guannan liu daniel Mullan Aimin Zhang Hui liu Dongcheng liu Guijun Yan UWA School of Agriculture and Environment and The UWA Institute of AgricultureThe University of Western AustraliaPerthWA 6009Australia InterGrain Pty.ltd. 19 Ambitious Link Bibra LakeWA 6163Australia State Key laboratory of North China Crop Improvement and Regulation College of AgronomyHebei Agriculture UniversityBaoding 071000HebeiChina

Common wheat(Triticum aestivum l.)is the most important crop in the world and a typical allopolyploid with a large and complex ***-harvest sprouting(PHS)leads to a significant reduction in grain quality *** is a complex trait with related QTl located on different ***,the study of markers and genes related to PHS resistance is limited especially for whitegrained *** pairs of near isogenic lines(NIls)from a white-grained wheat cross of CharaDM5637B*8 targeting a major QTl for PHS resistance(***-3A.1)on wheat chromosme 3Al were genotyped using the 90K SNP Illumina iSelect *** SNPs were identified,with a 75%-100%consistency between genotype and phenotype in the resistant or susceptible *** 10 SNPs were converted to cost-effective kompetitive allele-specific PCR(KASP)*** of 48 wheat cultivars with different phenotypes of PHS identified four KASP markers with 81.3%-85.4%conformity between genotype and *** investigation revealed that the four SNPs(BS00022245_51,Kukri_c49927_151,BS00022884_51 and BS00110550_51)corresponding to the four validated KASP markers are residing in three independent genes(TraesCS3A03G1072800,TraesCS3A03G1072400,TraesCS3A03G1071800)close to each other with a distance of 4.28-4.48 Mb to the targeted *** three annotated genes have potential functions related to PHS *** study revealed that combined use of NIls and the 90K SNP chip is a powerful approach for developing KASP markers and mining functional genes in *** KASP markers for PHS resistance on chromosome 3Al are useful for high-throughput evaluation and marker-assisted selection,and the three identified genes could lead to a better understanding of the genetic pathways controlling PHS.

关键词： Pre-harvest sprouting(PHS) KASP marker Functional genes Chromosome 3Al 90K SNP assay Near-isogenic lines

Taxonomy of Pseudolagarobasidium(Polyporales,Basidiomycota)

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Fungal Diversity 2012年第4期55卷 155-169页

作者： Karen K.Nakasone daniel l.lindner Center for Forest Mycology Research Northern Research StationU.S.Forest ServiceOne Gifford Pinchot DriveMadisonWI 53726-2398USA

Pseudolagarobasidium(Polyporales,Basidiomycota)is a small,monophyletic genus of crustose,wood-inhabiting fungi with spines and a saprobic,endophytic,or parasitic *** species are accepted in the genus including two new species,*** from Belize and *** from *** analysis of the internal transcribed spacer of the ribosomal RNA gene places *** in a monophyletic clade with *** and an undescribed foliar *** combinations proposed include *** for Irpex modestus Berk.,*** for Hydnum pronum Berk.&Broome which is an earlier name for ***,and *** for Radulodon venustus Hjortstam&*** colliculosum Berk.&Broome from Sri lanka is conspecific with *** species,Sistotrema ochroleucum and Radulum concentricum are not accepted in *** is compared with Radulodon and similar genera.A key to the species of Pseudolagarobasidium is provided.

关键词： Pirex concentricum Pseudolagarobasidium acaciicola P.leguminicola Hydnum ayresii Microbinding hyphae

The N-terminus of COX-1 and its effect on cyclooxygenase-1 catalytic activity

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Journal of Genetics and Genomics 2010年第2期37卷 117-123页

作者： Sean Phipps Michael J. Turner daniel l. Simmons Chemistry and Biochemistry Department Brigham Young University

Cyclooxygenases are encoded by COX-1 and COX-2. They share over sixty percent sequence identity in human and are similar to each other in their crystallographic structures. One major difference in the primary structure of these two isozymes is the presence of eight amino acids in the amino-terminal region of COX-1 that are not present in COX-2. The function of this amino acid sequence is unknown. In this study, a human COX-1 mutant （Δ7aa） with this sequence removed was studied in parallel with COX-1. Signal peptide cleavage, N-linked glycosylation, protein expression, distribution and dimerization were not affected by the mutation. The mutant was enzymati-cally active and showed the same sensitivity toward aspirin. The KM for the enzyme remained the same as COX-1. However, the Vmax of the COX-1 mutant decreased by 3.3-fold. We conclude that the COX-1 specific amino-terminal sequence has a subtle but detectable effect on COX-1 catalysis.

关键词： COX-1 COX-2 dimerization glycosylation aspirin KM Vmax

Mode of action for inorganic arsenic toxicity and carcinogenesis

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Chinese Science Bulletin 2014年第11期59卷 1078-1082页

作者： Puttappa R.Dodmane lora l.Arnold Samuel M.cohen Department of Pathology and Microbiology University of Nebraska Medical Center983135 Nebraska Medical Center

Inorganic arsenic(iAs) at high doses is a known human carcinogen, inducing tumors of the skin,urinary bladder, and lung. It is also associated with noncancer toxicities. An understanding of the mode of action(MOA) for arsenic-induced effects is needed to develop a scientifically-based risk assessment. To determine an MOA for iAs induced toxicities, it is necessary to understand the metabolism, kinetics, cell transport, and interaction with specific proteins of iAs. Based on in vitro investigations using animal and human cells, studies from animal models,and clinical and epidemiological studies, we have proposed an MOA involving formation of sufficient levels of reactive trivalent metabolites which interact with critical free sulfhydryl groups, leading to cytotoxicity and regenerative cell proliferation. There is a strong correlation between in vitro cytotoxicity([0.1 lmol/l trivalent arsenicals) and the no effect levels in rodents [approximately 1 ppm(1 ppm = 1 mg/l) of water or diet]. In epithelial target tissues, the cytotoxic effects of iAs result in chronic precursor lesions which have the potential for an increased risk of developing cancer. In non-epithelial tissues, noncancer toxicities such as hypertension and atherosclerosis develop. This MOA implies a non-linear, threshold dose–response relationship for both non-cancer and cancer end points of exposure to iAs.

关键词：体外细胞毒性致癌作用无机砷诱导效应会计准则氧化锌避雷器上皮组织动脉粥样硬化

Surface Reconstruction Techniques for Imperfect Raw-data

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Surface Reconstruction Techniques for Imperfect Raw-data

Effects of Carbon Content and Solidification Rate on the Thermal Conductivity of Grey Cast Iron

2009 11th IEEE International Conference on Computer-Aided Design and Computer Graphics(IEEE CAD/Graphics 2009)

作者： daniel cohen-Or School of Computer Science Tel Aviv University Israel

In my talk I will present some surface reconstruction techniques that handle imperfect *** data is common in raw scans and contains large missing parts,ghost outliers and under-sampled *** explore reconstruction techniques that focus on such problematic *** general goal is recovering the expected shape in terms of global topology and local geometric detail.

关键词： Surface reconstruction Wires Topology Shape Performance analysis Computer science Deformable models Reconstruction algorithms Noise reduction

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Tsinghua Science and Technology 2008年第2期13卷 170-176页

作者： daniel Holmgren Attila Diószegi Ingvar l. Svensson Department of Mechanical Engineering and Component Technology Jnkping University

The thermal conductivity/diffusivity of pearlitic grey irons with various carbon contents was investigated by the laser flash method. The materials were cast in controlled thermal environments producing three dissimilar cooling rates. The cooling rates together with the carbon content largely influence the thermal conductivity of grey iron. linear relationships exist between the thermal conductivity and the carbon content, the carbon equivalent, and the fraction of the former primary solidified austenite transformed into pearlite. The results show that the optimal thermal transport properties are obtained at medium cooling rates. Equations are given for the thermal conductivity of pearlite, solidified as pre-eutectic austenite, and the eutectic of grey iron. The thermal conductivity of pearlitic grey iron is modelled at both room temperature and elevated temperatures with good accuracy.

关键词： grey iron thermal conductivity thermal diffusivity carbon content carbon equivalent color etching graphite morphology eutectic cell size

Could diffuse coevolution explain the generic eggshell color of the brown-headed cowbird?

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Current Zoology 2021年第6期67卷 645-652页

作者： daniel HANlEY Beheshteh MOGHADDAME-JAFARI Samantha l.RUTlEDGE Department of Biology George Mason UniversityFairfaxVA 22030USA Department of Biology Mansfield University of PennsylvaniaMansfieldPA 16933USA Museum of Natural Science and Department of Biological Sciences Louisiana State UniversityBaton RougeLA 70803USA

The brown-headed cowbird(hereafter cowbird)is an avian brood parasite that produces an egg dissimilar to those produced by the majority of its diverse host *** cowbird’s generic egg may result from a Jack-of-all-trades strategy;however,the evolutionary mechanisms that select for their generic eggs are *** we propose that the cowbird’s eggshell phenotypes have evolved via diffuse coevolution,which results from community-level selective pressures,rather than via pairwise coevolution that occurs between a particular host species and its brood *** diffuse coevolution the cowbird’s host community,with varying eggshell phenotypes and recognition abilities,would select for a cowbird eggshell phenotype intermediate to those of its host *** selection is exerted by hosts that reject cowbird eggs,rather than those that accept them;therefore,we expect cowbird eggshell colors can be approximated by both the phenotypes and rejection abilities of their host *** we use eggshell reflectance data from 43 host species to demonstrate that the cowbird eggshell phenotypes are reasonably predicted(within 2 just noticeable differences)by the eggshell phenotypes and rejection rates of their *** findings suggest that cowbird eggshell phenotypes,and potentially those of other some generalist parasites,may evolve via diffuse ***,this research provides insight into the underlying evolutionary processes that explain observed phenotypic variation and provides a framework for studying selection on both specialist and generalist parasites’traits.

关键词： cowbird diffuse coevolution egg rejection