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作者： daniel cabezas montero. 浙江大学

学位级别：硕士

cDNA microarray consisting of 1330 unigenes derived from rice endosperm cDNA library was applied to monitor the transcript abundance of different tissues （ root, stem and leaf） in rice line Zhongzao 21. The results demonstrated that microarray could be used to monitor the expression levels of thousands of gene in different tissues simultaneously. By analyzing the results of hybridization we got one tissue-specific full-length cDNA fragment （H012d03）, which had none, trace and intensive expression level in tissues of root, stem and leaf respectively. Northern blotting confirmed the results. Electronic PCR results suggested that :his gene lie in the structural gene of 23S rRNA belong to rice chloroplast genome. No similar rice EST had been found in the public database. Some research has proved that there was one intron repeated gene encoding I-CPAI protein in 23S rRNA of chloroplast genome of green alga. The gene can transcript into mRNA with poly A tail and encode protein with bioactivity. In rice, we found it is the first time in the world that in the structural gene of 23S rRNA of chloroplast genome, there also have the intron repeated gene could transcript into mRNA with poly A tail. Meanwhile, Another cDNA microarray with 4370 unigenes from library of rice endosperm and leaf tissue was also used to detect the expression level of mRNA from stem tissue of rice treated by water and plant hormone ABA. Results showed that the expression levels of 5 genes were depressed by phytohormone ABA. Reverse Northern blotting confirmed that one of the 5 genes （H024g06） was really depressed by ABA. Bioinformatics analysis showed that the gene H024g06 is the same as cytochrome C gene. Previous researches revealed that cytochrome C gene was associated with such stress response as drought and coldness, while ABA could induce the stress response of plant. Therefore our results suggested that

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Potentiality of Vermicompost Humic Acids in Banana in Vitro Micropropagation Clone： Enano Guantanamero

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Journal of Environmental Science and Engineering(A) 2013年第11期2卷 677-685页

作者： Marcia B. Moya Fernandez Luis Eduardo Cosio-Vargas daniel cabezas montero Andres Calderin Garcia Dany Marrero Lopez Sandra Perez Alvarez Deperment of Plant Protection Agrarian University of Havana (Fructuoso Rodriguez Perez) Mayabeque 32 700 Cuba INIFAP (Nacional Research Center of Forest Agrarian and Livestock) Uruapan 60150 Mexico Havana Biofabric Mayabeque 32700 Cuba

The research was done at Havana Biofabric, Mayabeque Province. The vitro plants of banana （Musa sp） used came from the clone Enano Guantanamero （viand type）. They come from multiplication phase in a Murashige and MS （Skoog medium）. The objective of this work was to study the potentialities of HA （humic acids） for stimulation of rooting and cell division process, searching for completely synthetic hormone substitution due to its high cost. The multiplication medium contained thiamine （2 mg·L^-1）, myo-inositol （100 mg·L^-1）, sugar （30mg·L^-1）. By the use of HA, five treatments were used with total substitution of auxin and cytokinin （TI： HA （10 ***）; （T2： HA （20 mg·L^-1）; （T3： HA （30mg·L^-1）; （T4： HA （40 mg·L^-1）; （T5： HA （50 mg·L^-1） and the control with 6BAP （benzil amino purine）（4 mg·L^-1）＋ IBA （indol butyric acid）（0.65 mg·L^-1）, pH of the medium was adjust just before agar were add. The results showed that in the explants under HA treatments, elongation and cell multiplication were favored confirmed by explants great high, root number and length due to dry mass. The total protein content, reduced carbohydrate and peroxidases enzymatic activity were determine. The use of Vermicompost HA in the in vitro micropropagation of banana allow the elimination of rooting phase saving materials and be able to pass explants directly to Acclimatization phase.

关键词： Banana multiplication phase HA (Humic Acids) rooting.

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模块表达序列标签技术的建立及相关水稻cDNA阵列的应用

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中国水稻科学 2004年第1期18卷 1-6页

作者：庄晓峰李德葆 cabezas daniel 戴承恩娄沂春董海涛浙江大学农业与生物技术学院生物信息与基因网络研究组浙江杭州310029

提出了一种新的表达序列标签制作技术———模块表达序列标签 (M EST)技术 ,利用该技术短时间内分离完成1985条水稻cDNA片段 ,制备完成相应的水稻cDNA阵列。实验证实 :该技术体系能成功分离目的基因 ,正确检测基因表达丰度的改变 ,并具... 详细信息

提出了一种新的表达序列标签制作技术———模块表达序列标签 (M EST)技术 ,利用该技术短时间内分离完成1985条水稻cDNA片段 ,制备完成相应的水稻cDNA阵列。实验证实 :该技术体系能成功分离目的基因 ,正确检测基因表达丰度的改变 ,并具备通过对基因群体表达模式的监测以实现对生物学事件的精确描述。M EST概念是对EST技术的完善和重要补充 ,运用M EST技术能有效利用生物信息学知识 ,加快基因克隆速率 ,为基因功能的研究提供手段和工具。

关键词：蛋白模体蛋白模块表达序列标签 cDNA阵列水稻

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Prevalence of and risk factors for erectile dysfunction in young nondiabetic obese men:results from a regional study

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Asian Journal of Andrology 2020年第4期22卷 372-378页

作者： María Molina-Vega Maite Asenjo-Plaza María JoséBanderas-Donaire María Dolores Hernández-Ollero Silvia Rodríguez-Moreno Juan Jálvarez-Millán Pablo cabezas-Sanchez Fernando Cardona-Díaz Juan Alcaide-Torres Lourdes Garrido-Sánchez daniel Castellano-Castillo Francisco J Tinahones JoséC Fernández-García Department of Endocrinology and Nutrition Virgen de la Victoria University Hospital(IBIMA)Málaga UniversityMálaga 29010Spain Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición(CIBEROBN) Instituto de Salud Carlos IIIMadrid 28029Spain Cruz de Humilladero Primary Care Center Málaga 29010Spain Teatinos Primary Care Center Málaga 29010Spain Chemical Sanitary Consulting(CQS Lab) Madrid 28003Spain

Erectile dysfunction(ED),a condition closely related to cardiovascular morbidity and mortality,is frequently associated with *** this study,we aimed to determine the prevalence of ED and evaluate the associated risk factors in a cohort of 254 young(18–49 years)nondiabetic obese(body mass index[BMI]≥30 kg m−2)men from primary *** function(International Index of Erectile Function[IIEF-5]questionnaire),quality of life(Aging Males'Symptoms[AMS scale]),and body composition analysis(Tanita MC-180MA)were *** testosterone was determined using high-performance liquid chromatography–mass *** logistic regression analysis was used to study the factors associated with *** prevalence was 42.1%.Subjects with ED presented higher BMI,waist circumference,number of components of the metabolic syndrome,AMS score,insulin resistance,and a more unfavorable body composition than those without *** logistic regression analysis showed that a pathological AMS score(odds ratio[OR]:4.238,P40%of subjects.A pathological AMS score,the degree of obesity,and age were positively associated with ED,while elevated HDL-cholesterol levels were inversely associated with the odds of presenting *** prospective studies are needed to evaluate the long-term consequences of ED in this population.

关键词： Aging Males'Symptoms score erectile dysfunction International Index of Erectile Function-5 questionnaire obesity testosterone

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冻融法纯化水稻胚乳RNA及稻米品质重要相关基因表达谱研究

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中国水稻科学 2005年第2期19卷 105-110页

作者：张海燕董海涛姚海根向跃武谭学林方永启邓晔金庆超 cabezas daniel 姜玉新戴承恩阎文昭李德葆浙江大学农业与生物技术学院生物信息与基因网络研究组浙江杭州310029 嘉兴市农业科学研究院浙江嘉兴314016 四川省农业科学院生物技术核技术研究所四川成都610066 云南农业大学稻作研究所云南昆明650201

提出了一种从水稻胚乳组织中快速分离总RNA的方法,可以简便地去除RNA中存在的淀粉等多糖类杂质,所获的胚乳总RNA其质量可满足Northern印迹、微阵列制备等分子生物学实验要求。将从966个水稻品种的胚乳中分离所得的RNA制备RNA阵列,用于... 详细信息

提出了一种从水稻胚乳组织中快速分离总RNA的方法,可以简便地去除RNA中存在的淀粉等多糖类杂质,所获的胚乳总RNA其质量可满足Northern印迹、微阵列制备等分子生物学实验要求。将从966个水稻品种的胚乳中分离所得的RNA制备RNA阵列,用于稻米品质重要基因Wx基因的表达谱分析,结果证实:水稻Wx基因表达丰度总体上与水稻胚乳的直链淀粉含量呈正相关;应用于Northern印迹实验,表明在水稻胚乳组织中,淀粉分支酶Rbe1具有两个长度不同的同源基因,其中一个分子量大的为胚乳特异表达,分子量小的则在水稻各组织中共表达。

关键词：胚乳水稻品种 Wx基因稻米品质淀粉分支酶总RNA 直链淀粉含量特异表达分子生物学实验同源基因

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