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Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第4期17卷 247-261页

作者： Toshiko TAKAHASHI-INIGUEZ Nelly ABURTO-rodriguez Ana Laura vilchis-GONZALEZ Maria Elena FLORES Departamento de Biologla Molecular y Biotecnologia lnstituto de Investigaciones BiomddicasUniversidad Nacional Autonoma de MexicoMexicoD.F.04510Mexico

Malate dehydrogenase （MDH） is an enzyme widely distributed among living organisms and is a key protein in the central oxidative pathway. It catalyzes the interconversion between malate and oxaloacetate using NAD＋ or NADP＊ as a cofactor. Surprisingly, this enzyme has been extensively studied in eukaryotes but there are few reports about this enzyme in prokaryotes. It is necessary to review the relevant information to gain a better understanding of the function of this enzyme. Our review of the data generated from studies in bacteria shows much diversity in their molecular properties, including weight, oligomeric states, cofactor and substrate binding affinities, as well as differences in the direction of the enzymatic reaction. Furthermore, due to the importance of its function, the transcription and activity of this enzyme are rigorously regulated. Crystal structures of MDH from different bacterial sources led to the identification of the regions involved in substrate and cofactor binding and the residues important for the dimer-dimer interface. This structural information allows one to make direct modifications to improve the enzyme catalysis by increasing its activity, cofactor binding capacity, substrate specificity, and thermostability. A comparative analysis of the phylogenetic reconstruction of MDH reveals interesting facts about its evolutionary history, dividing this superfamily of proteins into two principle clades and establishing relationships between MDHs from different cellular compartments from archaea, bacteria, and eukaryotes.

关键词： Malate dehydrogenase Carbon metabolism Tricarboxylic acid cycle

When Giant Lymphangioma Meets Hydrops Fetalis: An Uncommon Case Report of Lower Extremity Involvement

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Maternal-Fetal Medicine 2024年第4期6卷 253-256页

作者： Ariana Marie Martin Andrea Paola Sanchez-Cantul Andrea Cantu-rodriguez Raul Martinez-Salinas Jesus damian Valdez-Bocanegra Department of School of Medicine and Health Sciences TecSalud Tecnológico de MonterreyMonterreyNuevo Leon 64849Mexico Department of Radiology Hospital Christus Muguerza Alta EspecialidadMonterreyNuevo Leon 64040Mexico Department of Obstetrics and Gynecology Tecnológico de MonterreyMonterreyNuevo Leon 64849Mexico.

Approximately 75%of lymphangiomas manifest in the head and neck region,with limb and bone involvement observed in only 2%of *** case report presents a rare instance of fetal lymphangioma associated with hydrops fetalis,affecting the left lower extremity and abdomen.A 22-year-old secundigravida woman was referred to the gynecology department at 31.2 weeks of *** fetal ultrasound revealed a live fetus with a large lymphangioma involving the left lower extremity,gluteal region,and *** ultrasound findings included subcutaneous edema,pericardial effusion,ascites,placentomegaly,and tricuspid *** clinical examination confirmed the diagnosis of lymphatic ***,only one other similar case has been reported in the English-language PubMed *** report adds to the medical literature as a rare instance of prenatally diagnosed lymphangioma-associated hydrops fetalis.

关键词： Lymphatic abnormalities Lower extremity lymphangioma Hydrops fetalis Ultrasonography Case report

Efficacy and long-term evaluation of intramyocardial injection of autologous CD34-enriched PBMSC in old myocardial infarction

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World Journal of Cardiovascular Diseases 2012年第4期2卷 283-290页

作者： Jose Luis Aceves Abel Archundia Araceli Paez Rafael vilchis Elvira Varela Emma rodriguez Guillermo Diaz Lourdes Flores-Luna Martha Alvarado Manuel Lopez Hernandez Luis Felipe Montano Felipe Masso Department of Cardiovascular Surgery National Medic Center 20 de Noviembre ISSSTEMexico CityMexico Department of Cellular Physiology National Institute of Cardiology“Ignacio Chavez”Mexico CityMexico Center of Population Health Research National Institute of Public HealthMexico CityMexico Department of Hematology National Medic Center 20 de Noviembre ISSSTEMexico CityMexico Immunobiology Laboratory Department of Cell and Tissue BiologySchool of Medicine UNAMMexico CityMexico

Aims: We have shown that autologous transplant of CD34+-enriched peripheral-blood mononuclear cells (PBMSC) could restore depressed myocardial function, and sustain adequate myocardial function 12 months after surgery in patients with old (>one year-old) myocardial infarction. Our aim is to report the long-term morbidity and mortality efficacy of this procedure. Methods and results: Seventy patients with an old anteroseptal myocardial infarction were followed for 2 to 7 years, 35 had a revascularization procedure and received an intra-myocardial injection of autologous CD34+-enriched PBMSC (8 × 108 mononuclear cells/ml including 3 × 107 CD34+ cells/ml)(Group A). Group B patients only had the revascularization. Abnormal pre-surgical values of LVEF (33.2% ± 4.8%), LVDV (178 ± 13.7 ml), LVSV (120 ± 16 m), LVDD (58.9 ± 3.84 mm), E and A waves without contractility in infarction area in group A patients improved to approximate normal values (50% ± 3% for LVEF;90 ± 9.3 ml for LVDV;80 ± 9.9 ml for LVSV;55.3 ± 3 mm for LVDD;5.2 ± 0.5 cm/s for E wave and 4.18 ± 0.3 cm/s for A wave) 1 year after the procedure and have remained unaltered for all the follow-up period. All the patients remain alive. Only seven patients have been readmitted to the hospital for non-myocardial related events. Group B only 11 patients continued alive to 5 years after surgery and LEVF never increased more than 6%, all of them with many hospitalizations (n ≥ 10) by heart failure events. Conclusion: Intramyocardial injection of CD34+ highly enriched PBSC represent an encouraging alternative for patients with severely scarred and dysfunctional myocardium.

关键词： Stem Cells Myocardial Infarction CD34+ Cells Revascularization Heart Failure Ventricular Function

Avocado Nutritional Diagnosis （Persea americana Mill.）＂Hass＂, Soil Fertility and Water Quality in Ocuituco, Morelos

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Journal of Chemistry and Chemical Engineering 2013年第11期7卷 1068-1073页

作者： Hector Sotelo-Nava Oscar Gabriel Villegas-Torres Martha Lilia Dominguez-Patint Elias Hernfindez Castro Agustin damian Nava Antonio Rodrlguez-Martmez Carlos Manuel Acosta-Duran Maria Andrade-rodriguez Iran Alia-Tej acal Faculty of Agricultural Sciences Universidad Aut6noma del Estado de Morelos Cuernavaca 62209 Mexico Faculty of Chemical Sciences and Engineering Universidad Aut6noma del Estado de Morelos Cuernavaca 62209 Mexico Unit of Agricultural and Environmental Sciences Universidad Autonoma de Guerrero Guerrero 39196 Mexico Engineering and Applied Science Research Center Universidad Autonoma del Estado de Morelos Cuernavaca 62209 Mexico

Morelos is the fourth in area cultivated and production volume of avocado. Of the 13 municipalities where it is farmed, Ocuituco represents 43.51% and Tetela del Volain 27.53%. Despite being one of the main fruit species in Morelos, no information related to the fertility of the soil where it is grown, the quality of water available for irrigation and nutritional status of this important fruit for the state. The study was conducted in the municipality of Ocuituco which identified three agro-habitats taking into account climate, landform, soil type and altitude. In each agro-habitat were carried out soil sampling to determine the physical and chemical characteristics based on the Mexican Official Standard NOM-021-SEMARNAT-2000, the chemical quality of water for irrigation as described by Richards （1954） and crop nutrient status of avocado ＂Hass＂ according to the methodology described by Maldonado （2002）. The soils had different physical and chemical characteristics, as well as the nutritional status of avocado trees in the three agro-habitats. The trees had excessive concentration of CI, Cu and Zn. Indices of optimal percentage deviation expressed different nutritional requirements. The water used for irrigation was of low salinity and sodium content, so it can be used without restrictions.

关键词： Soil fertility water quality nutrients Hass.