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Prevention Corona Virus = Peace

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装饰 2020年第3期 2-2页

作者： Klaczkiewicz damian

以Rev依赖性凋亡增强HIV-1 gp160的抗原性

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中华微生物学和免疫学杂志 2003年第4期23卷 257-261页

作者：施伟民 Paul UCameron damian jF Purcell 同济大学附属同济医院皮肤科 Microbiology and Immunology Department of the Melbourne University Macfalane Burnet Centre for Medical Research

目的　检测Rev(HIV的调控基因 )依赖性肿瘤坏死因子受体 (TNFR 1)和gp16 0双重表达质粒pDM12 8 TNFR 1(pT12 8)的凋亡诱导功能。方法　采用基因枪转导及流式细胞仪检测新质粒的表达功能。结果　新结构具有特异的选择性表达作用。当Rev... 详细信息

目的　检测Rev(HIV的调控基因 )依赖性肿瘤坏死因子受体 (TNFR 1)和gp16 0双重表达质粒pDM12 8 TNFR 1(pT12 8)的凋亡诱导功能。方法　采用基因枪转导及流式细胞仪检测新质粒的表达功能。结果　新结构具有特异的选择性表达作用。当Rev存在时 ,能间接表达TNFR 1,明显诱导HeLa细胞凋亡 ,使绿荧光细胞百分率非常显著地低于阴性对照 (P 0 .0 1)。单纯pT12 8不能直接表达TNFR 1,绿荧光细胞百分率非常显著地高于单纯pT6 0转染组 (P0 .0 1)。当AD8或pMD +pRnv存在并表达Rev时 ,pT12 8均能表达TNFR 1,杀伤HeLa细胞 ,绿荧光细胞百分率非常显著地低于阴性对照 (P <0 .0 1)。pT12 8与pRev或AD8转染人正常的角质生成细胞时 ,能表达TNFR 1,诱导细胞凋亡。培养 72h后 ,阴性对照和单纯pT12 8组的绿荧光角质生成细胞数皆显著地超过pT12 8+pRev和pT12 8+pAD8组 (P <0 .0 1)。结论　pT12

关键词： Rev依赖性凋亡 HIV-1gp160 抗原性肿瘤坏死因子受体-l 基因枪流式细胞仪

An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-Ha jeong jarno Hiemstra Patrick V.Blokzijl Rebeca damian-Ferrara Danilo Martins dos Santos jéssica H.L.da Fonseca Min-Ho Kang jihyun Kim Dilara Yilmaz-Aykut Mei L.L.Cham-Pérez jeroen Leijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National Laboratory for Agriculture(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

The influence of Sm_(2)O_(3)dopant on structure,morphology and transport critical current density of MgB_(2)wires investigated by using the transmission electron microscope

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journal of Magnesium and Alloys 2024年第12期12卷 5061-5078页

作者： Daniel Gajda MichałBabij Andrzej Zaleski Dogan Avci Fırat Karaboga Hakan Yetis Ibrahim Belenli Dariusz Zasada damian Szymanski Małgorzata Małecka Wojciech Gil Tomasz Czujko Institute of Low Temperature and Structure Research Polish Academy of Sciences(PAS)50-422 WroclawPoland Quatum Metrology Laboratory National Metrology˙Institute TÜB˙ITAK41470 KocaeliTurkey Mehmet Tanrikulu Vocational School of Health Services Bolu Abant Izzet Baysal University14030 BoluTurkey Department of Physics Bolu Abant Izzet Baysal University14280 BoluTurkey Institute of Materials Science and Engineering Military University of Technology00-908 WarsawPoland Faculty of Chemsitry University of Wrocław50-383 WrocławPoland

This study reports results for the morphology,crystal structure and critical parameters of Sm_(2)O_(3)-doped MgB_(2)wires with low and high initial filling *** transmission electron microscope(TEM)images were done for the longitudinal section of MgB_(2)*** results show that the Sm_(2)O_(3)admixture significantly changes the morphology of the MgB_(2)material,accelerates the formation of the MgB_(2)phase,does not form rectangular MgB_(2)crystallites,does not leave pure Mg,and forms Sm_(2)O_(3)areas of 10 nm and 20 *** effects of Sm_(2)O_(3)addition on MgB_(2)formation in superconducting wires were revealed in detail in this ***,Sm_(2)O_(3)causes the formation of point pinning regions that significantly increase the critical transport current density at the temperature range from 15 K to 30 *** TEM images point out that rectangular MgB_(2)crystallites are formed in undoped Mg B_(2)wires,which have not been previously reported XRPD results showed that short-term heating allowed obtaining a larger amount of MgB_(2)phase for the MgB_(2)wire with high initial filling *** the other hand,long heating time and high initial density slow down the creation of MgB_(2)phase when the Mg is in the solid state.

关键词： MgB_(2)wire Sm_(2)O_(3) Pinning TEM

Spontaneous Imbibition of Water and Determination of Effective Contact Angles in the Eagle Ford Shale Formation Using Neutron Imaging

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journal of Earth Science 2017年第5期28卷 874-887页

作者： Victoria H.DiStefano Michael C.Cheshire joanna McFarlane Lindsay M.Kolbus Richard E.Hale Edmund Perfect Hassina Z.Bilheux Louis j.Santodonato Daniel S.hussey David L.jacobson jacob M.LaManna Philip R.Bingham Vitaliy Starchenko Lawrence M.Anovitz Physical Sciences Directorate Chemical Sciences Division Oak Ridge National Laboratory Bredesen Center University of Tennessee Energy & Environmental Sciences Directorate Energy & Transportation Sciences Division Oak Ridge National Laboratory Neutron Sciences Directorate Chemical and Engineering Materials Division Oak Ridge National Laboratory Nuclear Science & Engineering Directorate Reactor & Nuclear Systems Division Oak Ridge National Laboratory Department of Earth and Planetary Science University of Tennessee Neutron Sciences Directorate Instrument and Source Division Oak Ridge National Laboratory Physical Measurement Laboratory National Institute of Standards and Technology Energy & Environmental Sciences Directorate Electrical & Electronics Systems Research DivisionOak Ridge National Laboratory

Understanding of fundamental processes and prediction of optimal parameters during the horizontal drilling and hydraulic fracturing process results in economically effective improvement of oil and natural gas extraction. Although modern analytical and computational models can capture fracture growth, there is a lack of experimental data on spontaneous imbibition and wettability in oil and gas reservoirs for the validation of further model development. In this work, we used neutron im- aging to measure the spontaneous imbibition of water into fractures of Eagle Ford shale with known geometries and fracture orientations. An analytical solution for a set of nonlinear second-order diffe- rential equations was applied to the measured imbibition data to determine effective contact angles. The analytical solution fit the measured imbibition data reasonably well and determined effective con- tact angles that were slightly higher than static contact angles due to effects of in-situ changes in veloci- ty, surface roughness, and heterogeneity of mineral surfaces on the fracture surface. Additionally, small fracture widths may have retarded imbibition and affected model fits, which suggests that aver- age fracture widths are not satisfactory for modeling imbibition in natural systems.

关键词： spontaneous imbibition effective contact angle neutron imaging Eagle Ford shale rock fractures.

朗格汉斯细胞特异性递呈在巨噬细胞亲和性人类免疫缺陷病毒感染中的作用

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中华医学杂志 2003年第16期83卷 1442-1446页

作者：施伟民 Paul U Cameron damian jF Purcell 同济大学附属同济医院中心实验室上海200065 Microbiology and immunology department of the Melbourne University Macfalane Burnet centre for medical research

目的　观察巨噬细胞亲和性HIV经皮肤感染的细胞学机制 ,区别巨噬细胞亲和性及T细胞亲和性HIV感染的差异。方法　以基因枪转染AD8及NL4 3分子克隆至皮肤 ,流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达 ,磁分离术分离CD80 + 和CD80 ... 详细信息

目的　观察巨噬细胞亲和性HIV经皮肤感染的细胞学机制 ,区别巨噬细胞亲和性及T细胞亲和性HIV感染的差异。方法　以基因枪转染AD8及NL4 3分子克隆至皮肤 ,流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达 ,磁分离术分离CD80 + 和CD80 -的游离细胞 ,进而以PCR和逆转录酶检测游走细胞中HIV对CD+ 4 T淋巴细胞的感染和表达。结果　AD8及NL4 3经基因枪直接转染皮肤后 ,表皮游走细胞皆显示HIV转染 ,可扩增到gag基因。只有AD8转染的游走细胞能进一步感染CD4 + T淋巴细胞 ,CD4 + T淋巴细胞可扩增到gag并表达逆转录酶 ;含NL4 3的游走细胞 ,不能感染CD4 + T淋巴细胞 ,不能扩增到gag,不能表达逆转录酶。AD8转染的 1/ 2log稀释的CD80 + 细胞能够感染CD4 + T淋巴细胞。即使 4次稀释后 ,仍能感染CD4 + T细胞 ,PCR扩增到gag并有大量逆转录酶表达 ,超过对照组的 2～ 3倍。AD8转染的CD80 -细胞仅第 1次稀释与CD4 + T细胞共孵可扩增到gag,仍可见逆转录酶表达。进一步稀释后不能扩增到gag。NL4 3转染的CD80 + 或CD80 -游走细胞与CD4 + T细胞共孵 ,皆不能扩增到gag ,也无逆转录酶表达。结论　直接转染AD8的CD80 + 的游走表皮细胞能特异性地感染CD4 + T细胞。这种特异性不仅依赖于细胞表面HIV受体的亲和性结合 ,还依赖于转?

关键词：朗格汉斯细胞特异性递呈巨噬细胞亲和性人类免疫缺陷病毒感染作用

影响马铃薯(Solanum tuberosum L.)试管薯形成的因素

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马铃薯杂志 1990年第2期4卷 117-124页

作者： G.hussey N.j.Stacey 胡云海

不断繁殖的马铃薯枝条在含有2.0mg/l苄氨基嘌呤(BAP)+6％蔗糖(8小时和24小时光照)的培养基中继代培养,6～8周内可诱导形成微型薯。BAP促进块茎形成的效果在短日照下比常日照更好。短日照下大多数块茎在琼脂表面形成,长日照下块茎在琼脂... 详细信息

不断繁殖的马铃薯枝条在含有2.0mg/l苄氨基嘌呤(BAP)+6％蔗糖(8小时和24小时光照)的培养基中继代培养,6～8周内可诱导形成微型薯。BAP促进块茎形成的效果在短日照下比常日照更好。短日照下大多数块茎在琼脂表面形成,长日照下块茎在琼脂中形成。在培养基中加入矮壮素(CCC)对促进块茎形成的效果极差,但CCC加强了BAP导致在琼脂块茎上面提早形成的效果。在培养基中不加激素的情况下,块茎最终发生在4～5个月,短日照下比长日照下形成得早。经一段长日照培养然后给予短日照和低温处理并不加速块茎的形成。脱落酸(ABA)对块茎形成的效果很小,赤霉素(GA_3)强烈抑制块茎形成。密封的培养容器也抑制块茎形成,但如果培养基中引入乙烯吸收剂则抑制会清除。

关键词：马铃薯育种试管薯形成

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英国可再生电力发展状况

电器工业 2014年第6期 45-49页

作者： joe hussey 张爽英国IT Power 机械工业北京电工技术经济研究所国际部

德国能源转型是从2000年歼始的，现在大家认为1．0版已经走到头了，在德围能效大会上，正式提出了所谓德国能源转型的2．0版。

关键词：电力发展可再生英国能源德国能效

侵袭性非霍奇金淋巴瘤18F-FDG PET/CT显像的肿瘤代谢参数及放射组学特征在预测疗效和生存时间中的作用

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中华核医学与分子影像杂志 2018年第11期38卷 775-776页

作者：唐军 Aatif Parvez Noam Tau Douglas hussey Manjula Maganti Ur Metser 上海交通大学医学院附属苏州九龙医院核医学科 joint Department of Medical Imaging Princess Margaret Cancer CentreUniversity Health Network Mount Sinai Hospital and Women's College Hospital University of Toronto

目的确定来自于F-脱氧葡萄糖(FDG) PET/CT (PET)显像的肿瘤代谢性参数和放射组学特征是否能预测侵袭性B细胞淋巴瘤患者的疗效和预后。方法经作者单位伦理委员会批准,该回顾性研究纳入82例侵袭性B细胞淋巴瘤患者,行PET显像分期。分析使... 详细信息

目的确定来自于F-脱氧葡萄糖(FDG) PET/CT (PET)显像的肿瘤代谢性参数和放射组学特征是否能预测侵袭性B细胞淋巴瘤患者的疗效和预后。方法经作者单位伦理委员会批准,该回顾性研究纳入82例侵袭性B细胞淋巴瘤患者,行PET显像分期。分析使用不同阈值获得的全身具有代表性的肿瘤部位的代谢肿瘤体积(MTV)和肿瘤放射组学特征,对获取的特征与疗效、无病生存时间(DFS)和总生存时间(OS)进行相关性分析。结果治疗结束后,66例(80.5%)患者完全缓解。与疗效相关的参数是治疗前肿瘤大于6 cm(P=0.026)、治疗后无大于1.5 cm的残余肿瘤(P=0.028)及标准摄取值(SUV)阈值取3和6时的全身MTV(P=0.015和0.009)。纹理特征均未能预测一线治疗方案的疗效,而某些纹理特征如灰阶非均匀性(GLNU)与DFS相关(P=0.013),峰度与OS相关(P=0.035)。结论侵袭性B细胞淋巴瘤患者的全身MTV与疗效相关,而肿瘤的纹理特征不能预测疗效,尽管有几个纹理特征与治疗后残余肿瘤的出现有关,也有某些纹理特征与DFS和OS有关。尚需在更多患者中进行前瞻性研究以确定这些参数的临床预测价值。

关键词： PET/CT 非霍奇金淋巴瘤纹理放射组学代谢肿瘤体积