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Phytotoxic effect of Sesbania virgata(Cav.) *** seeds of agronomic and forestry species

Journal of Forestry Research 2015年第2期26卷 339-346页

作者： Vera Lygia El Id Bárbara Vale da Costa daiane salete broch mignoni Marina Belloni Veronesi Kelly Simoes Marcia Regina Braga Nelson Augusto dos Santos Junior Seed Department Institute of Botany Plant Physiology and Biochemistry Department Institute of Botany

Sesbania virgata（Cav.） *** a pioneer species native to South America able to release allelochemicals that affect germination and development of other plant *** aim of this work was to evaluate the allelopathic effect of *** on the germination and development of co-occurring species from gallery forest and on agronomic *** forest native species,Enterolobium contortisiliquum（Vell.） Morong and Sapindus saponaria L.,and two agronomic species（these as control）,Oryza sativa *** Solanum lycopersicum *** used in the lab and greenhouse assays with seeds and leaf extracts of *** species were more affected than native species when co-germinated with seeds of *** germination percentage and speed germination index of the agronomic species were reduced in both in vitro and greenhouse *** the same assays,the seeds of native species showed no significant differences in the parameters ***,the initial growth of the four species assayed was affected,with reduction in plant length and shoot diameter followed by significant reduction in plantlet shoot and root *** the assays with irrigation of *** leaf extracts,no significant inhibitory effects on germination were observed for all *** and shoot diameter of the native species were not affected by the leaf extracts,nor were fresh and dry ***,these parameters increased in the agronomic species as they were irrigated with leaf *** on our data we conclude that seed leachates of *** affect germination and seedling development of other species,suggesting that its invasive behavior is due mainly to seed *** with less pronounced effects on native species,allelopathy of *** might also influence Interspecific competition in its natural environment.

关键词： Allelochemicals Allelopathy Catechin Fabaceae Gallery forest

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Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01

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Journal of Forestry Research 2020年第2期31卷 507-519页

作者： Gisela Manuela de Franca Bettencourt Carlos Ricardo Soccol Thais salete Giovanella Luziane Franciscon daiane Rigoni Kestring Isabel Rodrigues Gerhardt Juliana Degenhardt-Goldbach Department of Bioprocess Engineering and Biotechnology Federal University of ParanaAvenida Cel.Francisco Heraclito dos Santos210CuritibaParanaBrazil Department of Agronomy Federal University of ParanaRua dos Funcionarios1540CuritibaParanaBrazil Embrapa Forestry Estrada da RibeiraKm 111ColomboParanaBrazil Embrapa Agricultural Informatics Av.Andre Tosello209CampinasSao PauloBrazil

Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus;however,the efficiency is still low for most species,so many researchers still use seeds instead of clones as initial *** work aimed to develop a genetic transformation protocol,based on a highly efficient in vitro organogenesis protocol,for an Eucalyptus urophylla clone selected in our breeding *** growth regulators were evaluated for indirect organogenesis and *** a two-step protocol,the combination of callus induction media supplemented with 0.5 μM thidiazuron+0.5 μM naphthaleneacetic acid(NAA)and shoot induction media supplemented with 5.0 μM benzylaminopurine+1.0 lM NAA allowed up to 85.6%shoot formation with more shoots per explants when compared with other *** plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance.A RAPD analysis was used to check for somaclonal *** tests using 11 RAPD primers,we did not observe somaclonal variation in the in vitro stages evaluated.

关键词： Eucalyptus Genetic transformation In vitro regeneration Plant growth regulators Somaclonal variation

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