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Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort

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Molecular Plant 2024年第9期17卷 1439-1457页

作者： Song Wu Ana Luisa Malaco Morotti Jun Yang Ertao Wang Evangelos C.tatsis National Key Laboratory of Plant Molecular Genetics CASs Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChineseAcademy of SciencesShanghai 200032China University of Chinese Academy of Sciences Shanghai 200032China UEPAMS-CAS-JICs Centre of Excellencefor Plant anamlcroblalSclence Snangnal 200032Cnina

Hyperforin is the compound responsible for the effectiveness of ***'s wort(Hypericum perforatum)as an antidepressant,but its complete biosynthetic pathway remains *** discovery based on co-expression analysis of bulk RNA-sequencing data or genome mining failed to discover the missing steps in hyperforin *** this study,we sequenced the 1.54-Gb tetraploid *** genome assem-bled into 32 chromosomes with the scaffold N50 value of 42.44 *** single-cell RNA sequencing,we iden-tified a type of cell,“Hyper cells”,wherein hyperforin biosynthesis de novo takes place in both the leaves and *** pathway reconstitution in yeast and tobacco,we identified and characterized four transmembrane prenyltransferases(HpPT1-4)that are localized at the plastid envelope and complete the hyperforin biosynthetic *** hyperforin polycyclic scaffold is created by a reaction cascade involving an irregular isoprenoid coupling and a tandem *** findings reveal how and where hy-perforin is biosynthesized,enabling synthetic-biology reconstitution of the complete ***,this study not only deepens our comprehension of specialized metabolism at the cellularlevel but also provides strategic guidance for elucidation of the biosynthetic pathways of other specializied metabolites in plants.

关键词： hyperforin Hypericum perforatum de novo genome sequencing single-cell RNA sequencing biosyn-thetic pathway meroterpenoids

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Effect of growth hormone on colonic anastomosis after intraperitoneal administration of 5-fluorouracil, bleomycin and cisplatin: An experimental study

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2679-2688页

作者： Ioannis Lambrou Ioannis Mantzoros Orestis Ioannidis d.mitrios tatsis Elissavet Anestiadou Vasiliki Bisbinas Manousos-Georgios Pramateftakis Efstathios Kotidis Barbara driagka Ourania Kerasidou Savvas Symeonidis Stefanos Bitsianis Freideriki Sifaki Konstantinos Angelopoulos Haralabos demetriades Stamatios Angelopoulos Fourth department of Surgery Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece department of ENT Royal Cornwall Hospitals NHS TrustCornwall TR13LJUnited Kingdom

BACKGROUNd Growth hormone(GH)plays a crucial role in wound healing and tissue repair in postoperative *** particular,colonic anastomosis healing following colorectal surgery is impaired by numerous chemotherapy *** To investigate whether GH can improve the healing of a colonic anastomosis following the adverse effects of intraperitoneal administration of 5-fluorouracil(5-FU),bleomycin and *** Eighty Wistar rats underwent laparotomy and a 1 cm-resection of the transverse colon,followed by an end-to-end anastomosis under general *** rats were blindly allocated into four equal groups and administered a different daily intraperitoneal therapeutic regimen for 6 *** control group(A)received normal *** B received chemotherapy with 5-FU(20 mg/kg),bleomycin(4 mg/kg)and cisplatin(0.7 mg/kg).Group C received GH(2 mg/kg),and group d received the aforementioned combination chemotherapy and GH,as *** rats were sacrificed on the 7th postoperative day and the anastomoses were macroscopically and microscop-ically *** weight,bursting pressure,hydroxyproline levels and inflammation markers were *** All rats survived until the day of sacrifice,with no infections or other complications.A decrease in the body weight of group d rats was observed,not statistically significant compared to group A(P=1),but significantly different to groups C(P=0.001)and B(Pdehiscence rate was not statistically different between the *** pressure was not significantly different between groups A and d(P=1.0),whereas group B had a significantly lower bursting pressure compared to group d(Pd significantly more adhesions than group ***,as a measurement of collagen deposition,was significantly higher in group d compared to group B(Pd higher,but not statistically significant,compared to group *** changes in group d were recorded,compared to group A

关键词： Growth hormone Colonic anastomosis Adhesion Bursting pressure Collagen Hydroxyproline Inflammation Neoangiogenesis Chemotherapy

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A/d Restrictions (Errors) in Ultra-Wideband Impulse Radios

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International Journal of Communications, Network and System Sciences 2010年第5期3卷 425-429页

作者： Giorgos tatsis Constantinos Votis Vasilis Raptis Vasilis Christofilakis Panos Kostarakis 不详

Ultra-Wideband Impulse Radio (UWB-IR) technologies, although are relatively easy in transmission but they present difficulties in reception, in fact the reception of such waveform is a quite complicated matter. The main reason is that in fully digital receiver the received waveform must be sampled at a rate of several GHz. This paper focuses on the impact of the Analog to digital (A/d) conversion stage that is used to sample the received waveform. More specifically we focus on the impact of the two main parameters that affect the performance of the Software defined Radio (SdR) system. These parameters are the bit resolution and the time jittering. The influence of these parameters is deeply examined.

关键词： UWB Impulse Radio AdC Jitter Error Quantization Error

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The genomes of medicinal skullcaps reveal the polyphyletic origins of clerodane diterpene biosynthesis in the family Lamiaceae

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Molecular Plant 2023年第3期16卷 549-570页

作者： Haixiu Li Song Wu Ruoxi Lin Yiren Xiao Ana Luisa Malaco Morotti Ya Wang Meytal Galilee Haowen Qin Tao Huang Yong Zhao Xun Zhou Jun Yang Qing Zhao Angelos K.Kanellis Cathie Martin Evangelos C.tatsis National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China Novogene Bioinformatics Institute BeijingChina Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai 201602 Group of Biotechnology of Pharmaceutical Plants Lab.of PharmacognosyDepartment of Pharmaceutical SciencesAristotle University of Thessaloniki54124 ThessalonikiGreece John Innes Centre Norwich NR47UHUK CEPAMS-CAS-JIC Centre of Excellence for Plant and Microbial Sciences Shanghai 200032China

The presence of anticancer clerodane diterpenoids is a chemotaxonomic marker for the traditional Chinese medicinal plant Scutellaria barbata,although the molecular mechanisms behind clerodane biosynthesis are ***,we report a high-quality assembly of the 414.98 Mb genome of *** into 13 *** phylogenomic and biochemical data,we mapped the plastidial metabolism of kaurene(gibberellins),abietane,and clerodane diterpenes in three species of the family Lamiaceae(Scutellaria barbata,Scutellaria baicalensis,and Salvia splendens),facilitating the identification of genes involved in the biosynthesis of the clerodanes,kolavenol,and *** show that clerodane biosynthesis evolved through recruitment and neofunctionalization of genes from gibberellin and abietane *** the assumed monophyletic origin of clerodane biosynthesis,which is widespread in species of the Lamiaceae,our data show distinct evolutionary lineages and suggest polyphyletic origins of clerodane biosynthesis in the family *** study not only provides significant insights into the evolution of clerodane biosynthetic pathways in the mint family,Lamiaceae,but also will facilitate the production of anticancer clerodanes through future metabolic engineering efforts.

关键词： Scutellaria barbata genome clerodane diterpenoids biosynthesis specialized metabolism evolution

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diterpenoids from Scutellaria barbata induce tumour-selective cytotoxicity by taking the brakes off apoptosis

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Medicinal Plant Biology 2022年第1期1卷 8-23页

作者： Matthew L.Tomlinson Man Zhao Elaine J.Barclay Jie Li Haixiu Li Juri Felix Lionel Hill Gerhard Saalbach Martin Rejzek dongfeng Yang Qing Zhao Paul Kroon Wei Wang Yongping Bao Melanie-Jayne R.Howes Evangelos C.tatsis Cathie Martin The John Innes Centre NorwichNR47UHUnited Kingdom College of Bioengineering and Biotechnology Zhejiang University of TechnologyHangzhou 310058China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China Royal Botanic Gardens Kew RichmondSurreyLondon TW93ABUnited Kingdom College of Life Sciences Zhejiang Sci-Tech UniversityXiasha Higher Education ZoneHangzhou 310018China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai 201602China Quadram Institute Bioscience Norwich Research ParkNorwichNR47UAUnited Kingdom Norwich Medical School University of East AngliaNorwichNorfolkNR47TJUnited Kingdom CEPAMS:CAS-JIC Centre of Excellence in Plant and Microbial Sciences

Medicinal plants are an excellent source of structurally diverse,bio-active compounds with potential in the fight against *** of the most promising is Scutellaria barbata,prescribed traditionally for the treatment of *** A is the major diterpenoid,produced in specialized large,peltate trichomes on leaves of *** induces dose-dependent apoptosis,specifically in cancer *** major class of proteins down-regulated are pro-survival proteins,the Inhibitors of Apoptosis(IAPs),and IAP regulating *** propose that scutebarbatine A works by releasing the molecular brakes(the IAPs)on apoptosis in cell death-evading cancer *** between the cytotoxicity of methanolic extracts of *** leaves and decoctions(Ban Zhi Lian)prepared traditionally,showed substantially different chemical compositions and differential induction of *** suggest polyvalency between the constituents in both extracts,and ways to produce enhanced chemopreventive preparations for the treatment of cancer.

关键词： prescribed methanol structurally

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