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HASTY modulates miRNA biogenesis by linking pri-miRNA transcription and processing

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Molecular Plant 2021年第3期14卷 426-439页

作者： d.mian a.cambiagno Axel J.Giudicatti Agustin L.Arce delfina Gagliardi Lei Li Wei Yuan derek S.Lundberg detlef Weigel Pablo A.Manavella Instituto de Agrobiotecnologı´a del Litoral(CONICET-UNL) Ca´tedra de Biologı´a Celular y MolecularFacultad de Bioquı´mica y Ciencias Biolo´gicasUniversidad Nacional del Litoral3000 Santa FeArgentina department of Molecular Biology Max Planck Institute for Developmental Biology72076 T€ubingenGermany

Post-transcriptional gene silencing mediated by microRNAs(miRNAs)modulates numerous developmental and stress response *** the last two decades,HASTY(HST),the ortholog of human EXPORTIN 5,was considered to be a candidate protein that exports plant miRNAs from the nucleus to the ***,we report that HST functions in the miRNA pathway independent of its cargo-exporting activity in *** found that Arabidopsis mutants with impaired HST shuttling exhibit normal subcellular distribution of ***,protein-protein interaction and microscopy assays showed that HST directly interacts with the microprocessor core component dCL1 through its N-terminal ***,mass spectrometry analysis revealed that HST also interacts independently of its N-terminal domain with the mediator complex subunit *** experiments revealed that HST could act as a scaffold to facilitate the recruitment of dCL1 to genomic MIRNA loci by stabilizing the dCL1-MEd37 complex,which in turn promotes the transcription and proper processing of primary miRNA transcripts(primiRNAs).Taken together,these results suggest that HST is likely associated with the formation of the miRNA biogenesis complex at MIRNA genes,promoting the transcription and processing of primiRNAs rather than the direct export of processed miRNAs from the nucleus.

关键词： microRNA gene silencing miRNA nuclear export HASTY miRNA biogenesis

Mechanistic insights into the novel glucose-sensitive behavior of P(NIPAM-co-2-AAPBA)

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Science China Chemistry 2020年第3期63卷 377-385页

作者： Qingxian Wang mian Fu Ying Guan Tony d.James Yongjun Zhang Key Laboratory of Functional Polymer Materials and State Key Laboratory of Medicinal Chemical Biology Institute of Polymer ChemistryCollege of ChemistryNankai UniversityTianjin 300071China department of Chemistry University of BathClaverton DownBath BA27AYUK

A glucose-sensitive polymer,poly(N-isopropylacrylamide-co-2-acrylamidophenylboronic acid)(P(NIPAM-co-2-AAPBA)),was synthesized by reversible addition fragmentation chain transfer(RAFT)*** of glucose results in reduced solubility and hence increased turbidity,rather than the normal increase in solubility(decreased turbidity)observed for other PBA-based glucose-sensitive *** novel glucose-sensitive behavior is explained by a new mechanism,in which glucose acts as an additive and depresses the lower critical solution temperature(LCST)of the polymer,instead of increasing solubility by increasing the degree of ionization of the PBA *** and theoretic analysis for the influence of glucose on the thermal behavior of P(NIPAM-co-2-AAPBA)reveals that glucose depresses the LCST of P(NIPAM-co-2-AAPBA)copolymers in a two-stage manner,a fast decrease at low glucose concentrations followed by a slow decrease at high glucose *** low glucose concentrations,the binding of glucose with PBA groups on the polymer chain increases the number of glucose molecules proximal to the polymer which influences the thermal behavior of the polymer,causing a rapid decrease in ***,the transition occurs at a glucose concentration equal to the reciprocal of the binding constant between PBA and glucose,thus providing a novel method to determine the binding *** saccharides,including mannose,galactose and fructose,also depress the LCST of P(NIPAM-co-2-AAPBA)copolymer in the same way.

关键词： glucose-sensitive mechanism phenylboronic acid thermosensitive binding constant lower critical solution temperature polymers

dysfunction of synaptic endocytic trafficking in Parkinson's disease

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Neural Regeneration Research 2024年第12期19卷 2649-2660页

作者： Xin Yi Ng mian Cao Programme in Neuroscience and Behavioural disorders Duke-NUS Medical SchoolSingaporeSingapore department of Physiology National University of SingaporeSingaporeSingapore

Parkinson's disease is characterized by the selective degeneration of dopamine neurons in the nigrostriatal pathway and dopamine deficiency in the *** precise reasons behind the specific degeneration of these dopamine neurons remain largely *** investigations have identified over 20 causative PARK genes and 90 genomic risk loci associated with both familial and sporadic Parkinson's ***,several of these genes are linked to the synaptic vesicle recycling process,particularly the clathrinmediated endocytosis *** suggests that impaired synaptic vesicle recycling might represent an early feature of Parkinson's disease,followed by axonal degeneration and the eventual loss of dopamine cell bodies in the midbrain via a"dying back"***,several new animal and cellular models with Parkinson's disease-linked mutations affecting the endocytic pathway have been created and extensively *** models faithfully recapitulate certain Parkinson's disease-like features at the animal,circuit,and cellular levels,and exhibit defects in synaptic membrane trafficking,further supporting the findings from human genetics and clinical *** this review,we will first summarize the cellular and molecular findings from the models of two Parkinson's disease-linked clathrin uncoating proteins:auxilin(dNAJC6/PARK19)and synaptojanin 1(SYNJ1/PARK20).The mouse models carrying these two PARK gene mutations phenocopy each other with specific dopamine terminal pathology and display a potent synergistic ***,we will delve into the involvement of several clathrin-mediated endocytosis-related proteins(GAK,endophilin A1,SAC2/INPP5 F,synaptotagmin-11),identified as Parkinson's disease risk factors through genome-wide association studies,in Parkinson's disease *** will also explore the direct or indirect roles of some common Parkinson's disease-linked proteins(alpha-synuclein(PARK1/4),Parkin(PARK2),and LRRK2(PARK

关键词： autophagy auxilin/PARK19 clathrin-mediated endocytosis dopamine neurons neurodegeneration nigrostriatal pathway Parkinson's disease synaptic vesicle recycling synaptojanin1/PARK20

A Linear Operator Method to Compute the Normal Modes with Rotation of any Asymmetric 3d Planet with Pure Vector Spherical Harmonics

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Research in Astronomy and Astrophysics 2023年第10期23卷 224-254页

作者： mian Zhang Cheng-Li Huang CAS Key Laboratory of Planetary Sciences Shanghai Astronomical ObservatoryChinese Academy of SciencesShanghai 200030China School of Astronomy and Space Sciences University of Chinese Academy of SciencesBeijing 100049China

In order to compute the free core nutation of the terrestrial planets, such as Earth and Mars, the Moon and lower degree normal modes of the Jovian planets, we propose a linear operator method(LOM). Generalized surface spherical harmonics(GSSHs) are usually applied to the elliptical models with a stress tensor, which cannot be expressed in vector spherical harmonics explicitly. However, GSSHs involve complicated math. LOM is an alternative to GSSHs,whereas it only deals with the coupling fields of the same azimuthal order m, as is the case when a planet model is axially symmetric and rotates about that symmetry axis. We extend LOM to any asymmetric 3d model. The lower degree spheroidal modes of the Earth are computed to validate our method, and the results agree very well with what is observed. We also compute the normal modes of a two-layer Saturn model as a simple application.

关键词： planets and satellites:interiors Earth planets and satellites:gaseous planets

Towards semi-supervised myoelectric finger motion recognition based on spatial motor units activation

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Science China(Technological Sciences) 2022年第6期65卷 1232-1242页

作者： GUO WeiChao WANG mian SHENG XinJun ZHU XiangYang State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Meta Robotics Institute Shanghai Jiao Tong UniversityShanghai 200240China

It is vital to recognize the intention of finger motions for human-machine interaction(HMI).The latest research focuses on fine myoelectric control through the decoding of neural motor unit action potential trains(MUAPt) from high-density surface electromyographic(sEMG) ***,the existing EMG decoding algorithms rarely obtain the spatial matching relationship between decoded motion units(MU) and designated muscles,and the control interface can only recognize the trained hand *** this study,a semi-supervised HMI based on MU-muscle matching(MMM) is proposed to recognize individual finger motions and even the untrained combined multi-finger *** automatic channel selection from high-density s EMG signals,the optimal spatial positions to monitor the MU activation of finger muscles are *** tapping experiment is carried out on ten subjects,and the experimental results show that the proposed s EMG decomposition algorithm based on MMM can accurately identify single finger motions with an accuracy of 93.1%±1.4%,which is comparable to that of state-of-the-art pattern recognition ***,the MMM allows unsupervised recognizing the untrained combined multi-finger motions with an accuracy of 73%±3.8%.The outcomes of this study benefit the practical applications of HMI,such as controlling prosthetic hand and virtual keyboard.

关键词： myoelectric control semi-supervised motor units activation channel selection template matching

A NEW SOLUTION OF CHIRAL N=2,d=10 SUPERGRAVITY

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Chinese Physics Letters 1989年第4期6卷 145-148页

作者： JU Guoxin WANG mian department of Physics Henan Normal UniversiytXinxiang

We combine the Freund-Rubin(FR)type anaatz with the nonlinear sigma model,a new solution of the chiral N=2,d=10 supergravity compactified on manifold M^(4)×N^(4)×H^(2) is *** properties are discussed.

关键词： N=2 manifold chiral

Flow dynamics analyses of pathophysiological liver lobules using porous media theory

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Acta Mechanica Sinica 2017年第4期33卷 823-832页

作者： Jinrong Hu Shouqin Lü Shiliang Feng mian Long Center of Biomechanics and Bioengineering Key Laboratory of Microgravity (National Microgravity Laboratory) Beijing Key Laboratory of Engineered Construction and Mechanobiology Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Blood flow inside the liver plays a key role in hepatic functions, and abnormal hemodynamics are highly correlated with liver diseases. To date, the flow field in an elementary building block of the organ, the liver lobule,is difficult to determine experimentally in humans due to its complicated structure, with radially branched microvasculature and the technical difficulties that derive from its geometric constraints. Here we established a set of 3d computational models for a liver lobule using porous media theory and analyzed its flow dynamics in normal, fibrotic,and cirrhotic lobules. Our simulations indicated that those approximations of ordinary flow in portal tracts（PTs） and the central vein, and of porous media flow in the sinusoidal network, were reasonable only for normal or fibrotic *** modified with high resistance in PTs and collateral vessels inside sinusoids were able to describe the flow features in cirrhotic lobules. Pressures, average velocities, and volume flow rates were profiled and the predictions compared well with experimental data. This study furthered our understanding of the flow dynamics features of liver lobules and the differences among normal, fibrotic, and cirrhotic lobules.

关键词： porous cirrhotic collateral ordinary permeability tracts constraints branched porosity inlet

Mechanokinetics of receptor-ligand interactions in cell adhesion

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Acta Mechanica Sinica 2015年第2期31卷 248-258页

作者： Ning Li Shouqin Lü Yan Zhang mian Long Key Laboratory of Microgravity(National Microgravity Laboratory) Center of Biomechanics and Bioengineeringand Beijing Key Laboratory of Engineered Construction and MechanobiologyInstitute of MechanicsChinese Academy of Sciences

Receptor-ligand interactions in blood flow are crucial to initiate such biological processes as inflammatory cascade,platelet thrombosis,as well as tumor *** mediate cell adhesion,the interacting receptors and ligands must be anchored onto two apposing surfaces of two cells or a cell and a substratum,i.e.,two-dimensional（2d）binding,which is different from the binding of a soluble ligand in fluid phase to a receptor,i.e.,three-dimensional（3d） *** numerous works have been focused on3 d kinetics of receptor-ligand interactions in the immune system,2d kinetics and its regulations have been less understood,since no theoretical framework or experimental assays were established until *** only does the molecular structure dominate 2d binding kinetics,but the shear force in blood flow also regulates cell adhesion mediated by interacting receptors and ***,we provide an overview of current progress in 2d binding and regulations,mainly from our *** issues of theoretical frameworks,experimental measurements,kinetic rates and binding affinities,and force regulations are discussed.

关键词： Receptor-ligand interactions Selectins β_2 integrins 2d binding kinetics

Two new species of the genus Wieseria (Nematoda:Enoplaida:Oxystominidae) from the Jiaozhou Bay

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Acta Oceanologica Sinica 2018年第10期37卷 157-160页

作者： HUANG mian SUN Jing HUANG Yong College of Life Sciences Liaocheng University

Two new species of free-living marine nematodes of the genus Wieseria are described from the sublittoral sediment of the Jiaozhou Bay in Qingdao. Wieseria sinica sp. nov. is characterized by having short labial and cephalic setae in a backward direction; oblong amphidial foveas with double contour; arcuate spicules with alea; ring-like guberculum; and clavated tail. Wieseria tenuisa sp. nov. is characterized by its very large ＂a＂ value （133.6）, long labial setae in a forward direction; spicules with alea and hooked proximal end; rodlike gubernaculums; and relatively longer clavated tail （7.9 a.b.d, long）. It differs from Wieseria sinica sp. nov. mainly by the longer labial setae, slightly straight spicules with hooked proximal end, and its thinner body. Amended dichotomous key to male species of Wieseria is provided.

关键词： Wieseria sinica sp. nov. Wieseria tenuisa sp. nov. free-living marine nematode taxonomy