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Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution

Bio-design and Manufacturing 2024年第3期7卷 292-306页

作者： Giuliana Grasso Valentina Onesto Stefania Forciniti Eliana d’Amone Francesco Colella Lara Pierantoni Valeria Famà Giuseppe Gigli Rui L.Reis j.Miguel Oliveira Loretta L.del Mercato nstitute of Nanotechnology National Research Council(CNR-NANOTEC)c/o Campus Ecoteknevia Monteroni73100 LecceItaly department of Mathematics and Physics“Ennio de Giorgi” University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal department of Experimental Medicine University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly

Oxygen(O_(2))-sensing matrices are promising tools for the live monitoring of extracellular O_(2) consumption levels in long-term cell *** this study,ratiometric O_(2)-sensing membranes were prepared by electrospinning,an easy,low-cost,scalable,and robust method for fabricating ***(ε-caprolactone)and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)dichloride,which was used as the O_(2)-sensing probe,and rhodamine B isothiocyanate,which was used as the reference *** functionalized scaffolds were morphologically characterized by scanning electron microscopy,and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle *** sensing capabilities were investigated by confocal laser scanning microscopy,performing photobleaching,reversibility,and calibration curve studies toward different dissolved O_(2)(dO)*** sensing nanofibers showed a high response to changes in dO concentrations in the physiological-pathological range from 0.5%to 20%and good stability under ratiometric *** addition,the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,breast cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line Panc-1,thus recreating a suitable biological environment in *** O_(2)-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in ambient O_(2)content during drug testing/validation and tissue regeneration processes.

关键词： Electrospinning Ruthenium(II)dichloride Oxygen sensors Ratiometric imaging Fluorescence

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想象难以设想的事物:艺术镜头下的深时

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装饰 2024年第8期 49-61页

作者： [美]j.d.塔拉塞克汪芸(译) 美国国家科学院《装饰》

j.d.塔拉塞克是一位策展人、研究者和作家,他热衷于通过协作与综合性的工作探索艺术与科学的交叉点。他是美国国家科学院文化项目的负责人,该项目专注于探索科学、医学、技术和文化之间的相互关系;同时,他是《莱奥纳多》(麻省理工学院出... 详细信息

j.d.塔拉塞克是一位策展人、研究者和作家,他热衷于通过协作与综合性的工作探索艺术与科学的交叉点。他是美国国家科学院文化项目的负责人,该项目专注于探索科学、医学、技术和文化之间的相互关系;同时,他是《莱奥纳多》(麻省理工学院出版)的主编。塔拉塞克是dASER (华盛顿特区艺术科学晚会)的创始人和主持人,dASER是每个月在美国国家科学院举办的沙龙,他主持该系列活动已逾13载。此外,塔拉塞克还担任亚利桑那州立大学和美国国家科学院联合出版的《科学技术杂志》(Science and Technology Magazine)的艺术顾问。塔拉塞克在美国国家科学院策划了多个展览,其中包括“想象深时”(Imagining deep Time,2014年)、“梦幻解剖学”(Visionary Anatomies,在2004年至2006年期间在史密森学会巡展)、“吸收+传输:迈克与道格·斯塔恩的作品”(Absorption+Transmission:Work by Mike and doug Starn)。本文即塔拉塞克为“想象深时”展览撰写的文章,展览中的艺术家以代表了个人艺术探索的多样化的形式探索了“深时”理念。

关键词：美国国家科学院史密森学会联合出版麻省理工学院华盛顿特区塔拉艺术与科学艺术顾问

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In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

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Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović Aleksandar j.Krmpot Miroslav Živić Svetlana Savić-Šević Steva M.Lević Marina Stanić dejan Pantelić Brana jelenković Mihailo d.Rabasović Institute of Physiology and Biochemistry“Ivan djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

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Estradiol increases cortical and trabecular bone accrual and bone strength in an adolescent male-to-female mouse model of gender-affirming hormone therapy

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Bone Research 2024年第1期12卷 95-106页

作者： Tian Nie Varun S.Venkatesh Suzanne Golub Kathryn S.Stok Haniyeh Hemmatian Reena desai david j.Handelsman jeffrey d.Zajac Mathis Grossmann Rachel A.davey department of Medicine Austin HealthUniversity of MelbourneHeidelbergVIC 3084Australia department of Biomedical Engineering University of MelbourneParkvilleVIC 3010Australia ANZAC Research Institute University of Sydney and AndrologyConcord Repatriation General HospitalConcordNSW 2137Australia

The effects of gender-affirming hormone therapy on the skeletal integrity and fracture risk in transitioning adolescent trans girls are *** address this knowledge gap,we developed a mouse model to simulate male-to-female transition in human adolescents in whom puberty is first arrested by using gonadotrophin-releasing hormone analogs with subsequent estradiol *** was suppressed by orchidectomy in male mice at 5 weeks of *** 3 weeks post-surgery,male-to-female mice were treated with a high dose of estradiol(~0.85 mg)by intraperitoneal silastic implantation for 12 *** included intact and orchidectomized males at 3 weeks post-surgery,vehicle-treated intact males,intact females and orchidectomized males at 12 weeks *** to male controls,orchidectomized males exhibited decreased peak bone mass accrual and a decreased maximal force the bone could withstand prior to *** treatment in orchidectomized male-to-female mice compared to mice in all control groups was associated with an increased cortical thickness in the mid-diaphysis,while the periosteal circumference increased to a level that was intermediate between intact male and female controls,resulting in increased maximal force and *** trabecular bone,estradiol treatment increased newly formed trabeculae arising from the growth plate as well as mineralizing surface/bone surface and bone formation rate,consistent with the anabolic action of estradiol on osteoblast *** data support the concept that skeletal integrity can be preserved and that long-term fractures may be prevented in trans girls treated with GnRHa and a sufficiently high dose of *** study is needed to identify an optimal dose of estradiol that protects the bone without adverse side effects.

关键词： treatment treatment strength

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One-neutron stripping process in the^(209)Bi(^(6)Li,^(5)Li)^(210)Bi*reaction reaction

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Nuclear Science and Techniques 2024年第6期35卷 120-129页

作者： Gao-Long Zhang Zhen-Wei jiao Guang-Xin Zhang E.N.Cardozo B.Paes Shi-Peng Hu jian-Qiang Qian daniele Mengoni Wei-Wei Qu Cong-Bo Li Yun Zheng Huan-Qiao Zhang Hui-Bin Sun Nan Wang Chun-Lei Zhang j.j.Valiente-dobón d.Testov M.Mazzocco A.Gozzelino C.Parascandolo d.Pierroutsakou M.La Commara A.Goasduff d.Bazzacco d.R.Napoli F.Galtarossa F.Recchia A.Illana S.Bakes I.Zanon S.Aydin Gde Angelis M.Siciliano R.Menegazzo S.M.Lenzi S.Akkoyun L.F.Canto j.Lubian School of Physics Beihang UniversityBeijing 100191China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China Instituto de Física Universidade Federal Fluminense24210-340 NiteróiRio de JaneiroBrazil Laboratorio TANdAR Comisión Nacional de Energía AtómicaBKNA1650 San MartíınArgentina Institute for Advanced Study in Nuclear Energy and Safety Shenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Research and Manufacture of High Purity Germanium Materials and Fetectors Shenzhen UniversityShenzhen 518060China INFN Sezione di PadovaPaduaItaly dipartimento di fisica Astronomia dell’Universita di padova PaduaItaly State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China China Institute of Atomic Energy Beijing 102413China The Key Laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Laboratori Nazionali di Legnaro INFN35020 LegnaroItaly INFN Sezione di Napoli80126 NaplesItaly dipartimento di Farmacia Universitàdi Napoli“Federico II”80131 NaplesItaly department of Natural and Mathematical Sciences Faculty of EngineeringTarsus University33480 MersinTurkey Physics division Argonne National LaboratoryLemontILUSA department of Physics Sivas Cumhuriyet UniversitySivasTurkey Instituto de Física Universidade Federal do Rio de JaneiroRio de Janeiro CP 6852821941-972Brazil

One-neutron stripping process between^(6)Li and^(209)Bi was studied at 28,30,and 34 MeV using the in-beamγ-ray spectroscopy ***γ-γcoincident analysis clearly identified twoγ-rays feeding the ground and long-lived isomeric states,which were employed to determine the cross *** one-neutron stripping cross sections were similar to the cross sections of complete fusion in the^(6)Li+^(209)Bi system,but the one-neutron stripping cross sections decreased more gradually at the sub-barrier region.A coupled-reaction-channel calculation was performed to study the detailed reaction mechanism of the one-neutron stripping process in^(6)*** calculations indicated that the first excited state of 5 Li is critical in the actual one-neutron transfer mechanism,and the valence proton of 209Bi can be excited to the low-lying excited state in(^(6)Li,^(5)Li)reaction,unlike in the(d,p)reaction.

关键词： Transfer reaction Weakly bound nuclei Cross section

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Radioisotope production using lasers:From basic science to applications

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Matter and Radiation at Extremes 2024年第3期9卷 31-44页

作者： M.R.d.Rodrigues A.Bonasera M.Scisciò j.A.Pérez-Hernández M.Ehret F.Filippi P.L.Andreoli M.Huault H.Larreur d.Singappuli d.Molloy d.Raffestin M.Alonzo G.G.Rapisarda d.Lattuada G.L.Guardo C.Verona Fe.Consoli G.Petringa A.McNamee M.La Cognata S.Palmerini T.Carriere M.Cipriani G.di Giorgio G.Cristofari R.de Angelis G.A.P.Cirrone d.Margarone L.Giuffrida d.Batani P.Nicolai K.Batani R.Lera L.Volpe d.Giulietti S.Agarwal M.Krupka S.Singh Fa.Consoli Cyclotron Institute Texas A&M UniversityCollege StationTexas 77840USA Laboratori Nazionali del Sud Istituto Nazionale di Fisica Nucleare(LNS-INFN)CataniaItaly ENEA Fusion and Technologies for Nuclear Safety and Security Department-CFrascatiItaly Centro de Láseres Pulsados(clpu) VillamayorSpain Universidad de Salamanca SalamancaSpain Centre Lasers Intenses et Applications(CELIA) Universitéde BordeauxCNRSCEATalenceFrance HB11 Energy Holdings Pty FreshwaterNew South WalesAustralia School of Mathematics and Physics Queen’s University BelfastBelfastUnited Kingdom dipartimento di Fisica e Astronomia“E.Majorana ”Università di CataniaCataniaItaly Facoltàdi Ingegneria e Architettura Universitàdegli Studi di Enna“Kore”EnnaItaly dipartimento di Ingegneria Industriale Universitàdi Roma“Tor Vergata”RomaItaly dipartimento di Fisica e Geologia Universitàdegli Studi di PerugiaPerugiaItaly Istituto Nazionale di Fisica Nucleare Sezione di PerugiaPerugiaItaly ELI Beamlines Facility the Extreme Light Infrastructure ERICDolni BrezanyCzech Republic ETSIA Universidad Politécnica de MadridMadridSpain dipartimento Fisica “E.Fermi”Universitàdi Pisa and INFNPisaItaly FZU–Institute of Physics of Czech Academy of Sciences PragueCzech Republic Institute of Plasma Physics of Czech Academy of Sciences PragueCzech Republic

The discovery of chirped pulse amplification has led to great improvements in laser technology,enabling energetic laser beams to be compressed to pulse durations of tens of femtoseconds and focused to a few *** with energies of tens of MeV can be accelerated using,for instance,target normal sheath acceleration and focused on secondary *** such conditions,nuclear reactions can occur,with the production of radioisotopes suitable for medical *** use of high-repetition lasers to produce such isotopes is competitive with conventional methods mostly based on *** this paper,we study the production of^(67)Cu,^(63)Zn,^(18)F,and^(11)C,which are currently used in positron emission tomography and other *** the same time,we study the reactions^(10)B(p,α)^(7)Be and^(70)Zn(p,4n)^(67)Ga to put further constraints on the proton distributions at different angles,as well as the reaction^(11)B(p,α)^(8)Be relevant for energy *** experiment was performed at the 1 PW laser facility at VegaⅢin Salamanca,*** distributions of radioisotopes in the forward(with respect to the laser direction)and backward directions were measured using a high purity germanium *** results are in reasonable agreement with numerical estimates obtained following the approach of Kimura and Bonasera[*** ***.,Sect.A 637,164–170(2011)].

关键词： purity estimates backward

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Hepatic angiotensin-converting enzyme 2 expression in metabolic dysfunction-associated steatotic liver disease and in patients with fatal COVId-19

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World journal of Gastroenterology 2024年第31期30卷 3705-3716页

作者： Angus K jacobs Steven d Morley Kay Samuel Katie Morgan Lyndsey Boswell Timothy j Kendall david A dorward jonathan A Fallowfield Peter C Hayes john N Plevris Hepatology Laboratory University of EdinburghEdinburgh EH164SBUnited Kingdom Scottish National Blood Transfusion Service Jack Copland CentreEdinburgh EH144BEUnited Kingdom Institute for Regeneration and Repair University of EdinburghEdinburgh EH164UUUnited Kingdom Edinburgh Pathology University of EdinburghEdinburgh EH164SBUnited Kingdom

BACKGROUNd Metabolic dysfunction-associated steatotic liver disease(MASLd),characterised by hepatic lipid accumulation,causes inflammation and oxidative stress accompanied by cell damage and *** injury(LI)is also frequently reported in patients hospitalised with coronavirus disease 2019(COVId-19),while preexisting MASLd increases the risk of LI and the development of COVId-19-associated *** of injury at the cellular level remain unclear,but it may be significant that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)which causes COVId-19,uses angiotensin-converting expression enzyme 2(ACE2),a key regulator of the‘anti-inflammatory’arm of the renin-angiotensin system,for viral attachment and host cell *** To determine if hepatic ACE2 levels are altered during progression of MASLd and in patients who died with severe *** ACE2 protein levels and localisation,and histological fibrosis and lipid droplet accumulation as markers of MASLd were determined in formalin-fixed liver tissue sections across the MASLd pathological spectrum(isolated hepatocellular steatosis,metabolic dysfunction-associated steatohepatitis(MASH)+/-fibrosis,end-stage cirrhosis)and in post-mortem tissues from patients who had died with severe COVId-19,using ACE2 immunohistochemistry and haematoxylin and eosin and picrosirius red staining of total collagen and lipid droplet areas,followed by quantification using machine learning-based image pixel *** ACE2 staining is primarily intracellular and concentrated in the cytoplasm of centrilobular hepatocytes and apical membranes of bile duct ***,ACE2 protein levels are elevated in non-fibrotic MASH compared to healthy controls but not in the progression to MASH with fibrosis and in ***2 protein levels and histological fibrosis are not associated,but ACE2 and liver lipid droplet content are significantly correlated across the MASLd *** A

关键词： Metabolic dysfunction-associated steatotic liver disease Angiotensin-converting enzyme 2 Immunohistochemistry COVId-19 COVId-19-associated cholangiopathy

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Laboratory evaluation of toxicity of selected insecticides against egg and larval stages of cotton pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)

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journal of Cotton Research 2024年第1期7卷 15-28页

作者： BUSNOOR Abhishek V. WAdASKAR R.M. FANd Babasaheb B. TAMBE V.j. PILLAI T. MAHULE d.j. NAGRARE V.S. PRASAd Y.G. Entomology Section College of Agriculture(Dr.Panjabrao Deshmukh Krishi Vidyapeeth)Nagpur 440001MaharashtraIndia Indian Council of Agricultural Research-Central Institute for Cotton Research Nagpur 441108MaharashtraIndia

Background The cryptic nature of pink bollworm Pectinophora gossypiella(Saunders)larvae enables its reduced vul-nerability to insecticidal ***,the development of resistance against Bacillus thuringiensis(Bt)toxins posed a serious threat to transgenic cotton *** necessitated determining the critical timing of spray applications on the control *** study assessed the influence of egg age(freshly laid ***-day-old)and the loca-tion of larvae(directly exposed to the insecticide residues on the boll rind *** inside the bolls)on insecticide control *** The results revealed a significant decrease in the ovicidal activity of tested insecticides with an increase in the age of eggs from one day old to three days old(paired t-test,Pdirectly exposed to the insec-ticide residues on the boll rind were more susceptible(>80%mortality)than the larvae exposed after they had bur-rowed inside the bolls(d insecticides on developmental biology were more pronounced in the experiment on pre-larval release insecticide treatment compared with insecticide treatment given post-larval release and entry inside the *** Egg age influences the insecticide susceptibility,as does the larval location,directly exposed vs bur-rowed inside the *** eggs and the larvae that had burrowed inside the green bolls of cotton were relatively less susceptible to the insecticide *** toxic effects of insecticides on egg and larval stages were primar-ily *** findings are significant for devising a comprehensive strategy for pink bollworm management on a sustainable basis.

关键词： Bioefficacy Cotton Insecticides Pink bollworm Pectinophora gossypiella Timings of spray

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Temperature and structure measurements of heavy-ion-heated diamond using in situ X-ray diagnostics

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Matter and Radiation at Extremes 2024年第4期9卷 80-89页

作者： j.Lutgert P.Hesselbach M.Schorner V.Bagnoud R.Belikov P.drechsel B.Heuser O.SHumphries P.Katrik B.Lindqvist C.Qu R.Redmer d.Riley G.Schaumann S.Schumacher A.Tauschwitz d.Varentsov K.Weyrich X.Yu B.Zielbauer Zs.Major P.Neumayer d.Kraus Institut fur Physik Universitat RostockAlbert-Einstein-Str.2318059 RostockGermany GSI Helmholtzzentrum fur Schwerionenforschung Planckstr.164291 DarmstadtGermany Institut für Angewandte Physik Goethe-Universität Frankfurt am MainMax-von-Laue-Str.160438 Frankfurt am MainGermany Institut fur Geowissenschaften Goethe-Universitat Frankfurt am MainAltenhoferallee 160438 Frankfurt am MainGermany Helmholtz-Zentrum dresden-Rossendorf Bautzner Landstr.40001328 DresdenGermany European XFEL GmbH Holzkoppel 422869 SchenefeldGermany Centre for Plasma Physics School of Mathematics and PhysicsQueen’s University BelfastUniversity RoadBelfast BT71NNUnited Kingdom Institut für Kernphysik Technische Universität DarmstadtSchlossgartenstr.964289 DarmstadtGermany State Key Laboratory of Nuclear Physics and Technology Institute of Heavy Ion PhysicsPeking UniversityBeijing 100871China Helmholtz Institut jena Frobelstieg 307743 JenaGermany

We present in situ measurements of spectrally resolved X-ray scattering and X-ray diffraction from monocrystalline diamond samples heatedwith an intense pulse of heavy *** this way,we determine the samples’heating dynamics and their microscopic and macroscopic structuralintegrity over a timespan of several *** the ratio of elastic to inelastic scattering with state-of-the-art density functionaltheory molecular dynamics simulations allows the inference of average temperatures around 1300 K,in agreement with predictions fromstopping power *** simultaneous diffraction measurements show no hints of any volumetric graphitization of the material,butdo indicate the onset of fracture in the diamond *** experiments pave the way for future studies at the Facility for Antiproton andIon Research,where a substantially increased intensity of the heavy ion beam will be available.

关键词： heated intense structure

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A multi-shot target wheel assembly for high-repetition-rate, laser-driven proton acceleration

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High Power Laser Science and Engineering 2024年第02期 91-98页

作者： j.Pe?as A.Bembibre d.Cortina-Gil L.Martín A.Reija C.Ruiz M.Seimetz A.Alejo j.Benlliure Instituto Galego de Física de Altas Enerxías Universidade de Santiago de Compostela Laboratorio Láser de Aceleración y Aplicaciones RIAIDT Universidade de Santiago de Compostela Instituto Universitario de Física Fundamental y Matemáticas y dpto.de didáctica de la Matemática y de las Ciencias Experimentales Universidad de Salamanca Instituto de Instrumentación para Imagen Molecular (I3M) CSIC-Universitat Politècnica de València

A multi-shot target assembly and automatic alignment procedure for laser–plasma proton acceleration at high repetition rate are introduced. The assembly is based on a multi-target rotating wheel capable of hosting more than 5000 targets,mounted on a 3d motorized stage to allow rapid replenishment and alignment of the target material between laser irradiations. The automatic alignment procedure consists of a detailed mapping of the impact positions at the target surface prior to the irradiation that ensures stable operation of the target, which alongside the purpose-built design of the target wheel, enables operation at rates up to 10 Hz. Stable and continuous laser-driven proton acceleration at 10 Hz is demonstrated, with observed cut-off energy stability about 15%.

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