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A continental back-arc setting for the Namaqua belt:Evidence from the Kakamas domain

Geoscience Frontiers 2022年第4期13卷 193-221页

作者： P.H.Macey R.J.Thomas A.F.M.Kisters J.F.A.diener M.Angombe S.doggart C.A.Groenewald C.W.Lambert J.A.Miller H.Minnaar H.Smith H.F.G.Moen E.Muvangua A.Nguno G.Shifotoka J.Indongo d.frei C.Spencer P.le Roux R.A.Armstrong C.Tinguely Council for Geoscience P.O.Box 572BellvilleSouth Africa department of Geology University of StellenboschSouth Africa department of Geological Sciences University of Cape TownSouth Africa Geological Survey of Namibia WindhoekNamibia department of Geology University of Free StateSouth Africa department of Earth Sciences University of the Western CapeSouth Africa The Institute of Geoscience Research Dept.Applied GeologyCurtin UniversityPerthAustralia department of Geological Sciences and Geological Engineering Queen’s UniversityKingstonCanada Geochronology and Tracers Facility British Geological SurveyNottingham NG125GGUK RSES Australian NationalUniversityCanberraACTAustralia

A study of the NW Kakamas domain in South Africa/Namibia provides a new,unified lithostratigraphy and evolutionary history applicable to the whole Namaqua *** Mesoproterozoic history ranges from~1350 Ma to 960 Ma,but isotopic evidence suggests it was built upon pre-existing Paleoproterozoic continental crust that extended west from the Archaean *** eastern Namaqualand,early rift-related magmatism and sedimentation at~1350 Ma occurred in a confined ocean *** tectonic reversal and subduction at~1290–1240 Ma led to establishment of the Areachap,Konkiep and Kaaien *** the Kakamas domain,widespread deposition of pelitic sediments occurred at~1220 Ma(Narries Group).These contain detrital zircons derived from proximal crust with ages between~2020 Ma and 1800 Ma(western Palaeoproterozoic domains)and 1350–1240 Ma(eastern early Namaqua domains),suggesting pre-sedimentation *** pelites underwent granulite grade metamorphism at~1210 Ma(peak conditions:4.5–6 kbar and 770–850°C),associated with voluminous,predominantly S-type granitoid orthogneisses between~1210 Ma and 1190 Ma(Eendoorn and Ham River Suites)and low-angle ductile(d_(2))deformation which continued until~1110 Ma,interspersed with periods of *** enduring P-T regime is inconsistent with the expected crustal over-thickening associated with the generally-accepted collision-accretion Namaqualand ***,we propose the Namaqua Sector is a‘hot orogen’developed in a wide continental back-arc with subduction west of the present-day *** observed high geotherm resulted from thinned back-arc lithosphere accompanied by an influx of mantle-derived *** d_(2)deformation resulted from"bottom-driven"tectonics and viscous drag within the crust by convective flow in the underlying asthenospheric *** extended tectonothermal regime ceased at~1110 Ma when SW-directed thrusting stacked the Namaqua domains into their current positions,constrained in

关键词： Kakamas Namaqua Geodynamics Geochronology Hot orogeny Mesoproterozoic

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ITPK1 is an InsP_(6)/AdP phosphotransferase that controls phosphate signaling in Arabidopsis

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Molecular Plant 2024年第7期17卷 1151-1157页

(Molecular Plant 14,1864-1880;November 12021)We identified a mistake made when calculating the absolute concentrations of inositol(pyro)phosphates derived from CE-ESl-MS measurements reported in the originally published version of our *** to a calculation error when determining concentrations per fresh biomass,the absolute values shown in the manuscript are approximately 30-fold lower than they should *** mistake affects Figures 2d,3C,4C,and 7d and Figures S4,S11B,S13,and *** all conclusions made in the article referred to relative differences between genotypes and treatments,they remained unaffected by these mistakes.

关键词： ipt Arabidopsis absolute

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Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

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Synthetic and Systems Biotechnology 2019年第4期4卷 220-224页

作者： J.Porter Hunt Emily Long Zhao Mehran Soltani Madison frei J.Andrew d.Nelson Bradley C.Bundy department of Chemical Engineering Brigham Young UniversityProvoUTUSA department of Chemical Engineering Brigham Young University330N Engineering BuildingProvoUT84602USA

An“endotoxin-free”***-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and ***,preparation of the most complex CFPS reagent–the cell extract–remains time-consuming and labor-intensive because of the relatively slow growth kinetics of the endotoxin-free ClearColiTMBL21(dE3)*** we report a streamlined procedure for preparing *** cell extract from ClearColi™using auto-induction *** this work,the term auto-induction describes cell culture media which eliminates the need for manual induction of protein *** Clearcoli™cells in autoinduction media significantly reduces the hands-on time required during extract preparation,and the resulting“endotoxinfree”cell extract maintained the same cell-free protein synthesis capability as extract produced with traditional induction as demonstrated by the high-yield expression of crisantaspase,an FdA approved leukemia *** is anticipated that this work will lower the barrier for researchers to enter the field and use this technology as the method to produce endotoxin-free ***-based extract for CFPS.

关键词： Auto-induction Autoinduction Cell-free protein synthesis CFPS Endotoxin-free Crisantaspase Clearcoli

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An Update on Abscisic Acid Signaling in Plants and More

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Molecular Plant 2008年第2期1卷 198-217页

作者： Aleksandra Wasilewsk.a Florina Vlad Caroline Sirichandra Yulia Redko Fabien Jammes Christiane Valon Nicolas frei dit Frey Jeffrey Leung Institut des Sciences du Vegetal Centre National de la Recherche Scientifique UPR 23551 Avenue de la Terrasse Bat. 23 91190 Gif-sur-Yvette France CNRS UPR9073 Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie 75005 Paris France Laboratoire Signalisation et Regulation Coordonnee du Metabolisme Carbone et Azote Institut de Biotechnologie des Plantes (UMR8618) Universite Paris-Sud F-91405 Orsay Cedex France

The mode of abscisic acid （ABA） action, and its relations to drought adaptive responses in particular, has been a captivating area of plant hormone research for much over a decade. The hormone triggers stomatal closure to limit water loss through transpiration, as well as mobilizes a battery of genes that presumably serve to protect the cells from the ensuing oxidative damage in prolonged stress. The signaling network orchestrating these various responses is, however, highly complex. This review summarizes several significant advances made within the last few years. The biosynthetic pathway of the hormone is now almost completely elucidated, with the latest identification of the ABA4 gene encoding a neoxanthin synthase, which seems essential for de novo ABA biosynthesis during water stress. This leads to the interesting question on how ABA is then delivered to perception sites. In this respect, regulated transport has attracted renewed focus by the unexpected finding of a shoot-to-root translocation of ABA during drought response, and at the cellular level, by the identification of a B-galactosidase that releases biologically active ABA from inactive ABA-glucose ester. Surprising candidate ABA receptors were also identified in the form of the Flowering Time Control Protein A （FCA） and the Chloroplastic Magnesium Protoporphyrin-IX Chelatase H subunit （CHLH） in chloroplast-nucleus communication, both of which have been shown to bind ABA in vitro. On the other hand, the protein（s） corresponding to the physiologically detectable cell-surface ABA receptor（s） is （are） still not known with certainty. Genetic and physiological studies based on the guard cell have reinforced the central importance of reversible phosphorylation in modulating rapid ABA responses. Sucrose Non-Fermenting Related Kinases （SnRK）, Calcium-dependent Protein Kinases （CdPK）, Protein Phosphatases （PP） of the 2C and 2A classes figure as prominent regulators in this single-cell model. Identifying their d

关键词： Abscisic Acid Signaling Plants and More

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An Innovative Architectural Strategy for the Integration of Energy Issues into the design Process of a Commercial Center in Switzerland

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Journal of Building Construction and Planning Research 2014年第2期2卷 83-95页

作者： Emmanuel Rey Willi frei Sophie Lufkin dario Aiulfi Bauart Architectes et Urbanistes SA Neuchatel Switzerland Laboratory of Architecture and Sustainable Technologies (LAST) Institute of Architecture and the City (IA) School of Architecture Civil and Environmental Engineering (ENAC) Ecole polytechnique fédérale de Lausanne (EPFL) Lausanne Switzerland Laboratory of Architecture and Sustainable Technologies (LAST) Institute of Architecture and the City (IA) School of Architecture Civil and Environmental Engineering (ENAC) Ecole polytechnique fédérale de Lausanne (EPFL) Lausanne Switzerland Bauart Architectes et Urbanistes SA Neuchatel Switzerland Sorane SA Ecublens Switzerland

In a context of growing efforts to develop sustainability strategies, energy-related issues occupy central stage in the built environment. Thus, the energy performance of housings has improved radically over the past decades. Yet other types of buildings, in particular commercial centers, haven’t received the same level of interest. As a result, there is a need for effective and practical measures to decrease their energy consumption, both for heating and electricity. The objective of the paper is to demonstrate that it is possible, through coherent strategies, to integrate energy issues and bioclimatic principles into the design process of commercial centers. It analyzes the exemplary case study of Marin Commercial Center (Switzerland). The interdisciplinary approach, based on integrated design strategies, aimed at increasing the energy efficiency while keeping the cost comparable to the market cost. The main design principles include natural ventilation, nighttime cooling with energy recovery and natural lighting, as well as optimization of mechanical systems. The results of the simulations show that Marin Center attains the best energy performance observed so far among Swiss commercial centers. It also meets the Swiss Minergie standard. The paper thus questions traditional design processes and outlines the need for interdisciplinary evaluation and monitoring approaches tailored for commercial centers. Even though most crucial decisions are taken during the early stages, all phases of the process require systematic optimization strategies, especially operating stages. Recommendations include legal measures, in particular in the fields of ventilation and air-conditioning, education, professional development and technology transfer, and financial incentives for the replacement of energy intensive installations.

关键词： Energy Efficiency Commercial Centers design Process Sustainable Architecture Building Performance

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瑞士的外交政策

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丛书名：《瑞士史》

1988年

作者：（瑞士）弗　雷（frei d.）刘文立译

弗雷(1940- ),苏黎世大学讲授政治科学并担任瑞士国际研究院的领导工作:本书探讨了导致瑞士中立的地理、历史环境、瑞士中立的特点、瑞士中立在当代世界的意义等问题。

ISBN: (纸本)7562201471

关键词：对外政策地点:瑞士

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New Strigolactone Analogs as Plant Hormones with Low Activities in the Rhizosphere

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Molecular Plant 2014年第4期7卷 675-690页

作者： Francois-didier Boyer Alexandre de Saint Germain Jean-Bernard Pouvreau Guillaume Clave Jean-Paul Pillot Amelie Roux Amanda Rasmussen Stephen depuydt dominique Lauresserguesh Nicolas frei dit Frey Thomas S.A. Heugebaert Christian V. Stevens danny Geelen Sofie Goormachtig Catherine Rameau Institut Jean-Pierre Bourgin UMR1318 Institut National de la Recherche Agronomique (INRA)-AgroParisTech Route de Saint-Cyr (RD 10) F-78026 Versailles Cedex France Centre de Recherche de Gif Institut de Chimie des Substances Naturelles UPR2301 Centre National de la Recherche Scientifique (CNRS) 1 avenue de la Terrasse F-91198 Gif-sur-Yvette Cedex France Laboratoire de Biologie et de Pathologie Vegetales EA 1157 SFR 4207 QUASAV Universite de Nantes 44322 Nantes France Plant Production Faculty of Bioscience Engineering Ghent University Coupure links 653 Ghent 9000 Belgium Present address: School of Biosciences Division of Plant and Crop Production The University of Nottingham Sutton Bonington Leicestershire LE12 5RD UK department of Plant Systems Biology VIB Ghent 9052 Belgium department of Plant Biotechnology and Bioinformatics Ghent University Ghent 9052 Belgium Laboratoire de Recherche en Sciences Vegetales UMR 5546 Universite de Toulouse- Centre National de la Recherche Scientifique (CNRS) BP 42617 Auzeville F-31326 Castanet-Tolosan France Research Group SynBioC Department of Sustainable Organic Chemistry and Technology Faculty of Bioscience Engineering Ghent University Coupure links 653 B-9000 Gent Belgium

Strigolactones （SLs） are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and is also useful to control plant architectures without favoring the development of parasitic plants. Two different molecules （23 （3＇-methyI-GR24）, 31 （thia-3＇-methyl-debranone-like molecule）） already described, and a new one （AR36）, for which the synthesis is presented, are biologically compared with the well-known GR24 and the recently identified CISA-1. These different structures emphasize the wide range of parts attached to the d-ring for the bioactivity as a plant hormone. These new compounds possess a common dimethylbutenolide motif but their structure varies in the ABC part of the molecules： 23 has the same ABC part as GR24, while 31 and AR36 carry, respectively, an aromatic ring and an acyclic carbon chain. detailed information is given for the bioactivity of such derivatives in strigolactone synthesis or in perception mutant plants （pea rmsl and rms4, Arabidopsis max2 and, max4） for different hormonal functions along with their action in the rhizosphere on arbuscular mycorrhizal hyphal growth and parasitic weed germination.

关键词： strigolactones synthetic analogs Pisum sativum Arabidopsis root parasitic plants Gigaspora rosea plant growth regulator.

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ITPK1 is an InsP_(6)/AdP phosphotransferase that controls phosphate signaling in Arabidopsis.

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Molecular Plant 2021年第11期14卷 1864-1880页

作者： Esther Riemer danye Qiu debabrata Laha Robert K.Harmel Philipp Gaugler Verena Gaugler Michael frei Mohammad-Reza Hajirezaei Nargis Parvin Laha Lukas Krusenbaum Robin Schneider Adolfo Saiardi dorothea Fiedler Henning J.Jessen Gabriel Schaaf Ricardo F.H.Giehl department of Plant Nutrition Institute of Crop Science and Resource ConservationRheinische Friedrich-Wilhelms-Universitat Bonn53115 BonnGermany department of Chemistry and Pharmacy and CIBSS-Centre for Integrative Biological Signalling Studies Albert-Ludwigs University Freiburg79104 FreiburgGermany Medical Research Council Laboratory for Molecular Cell Biology(MRC-LMCB) University College LondonLondon WC1E 6BTUK department of Biochemistry Indian Institute of ScienceBengaluruKarnataka 560012India Leibniz-Forschungsinstitut fur Molekulare Pharmakologie 13125 BerlinGermany department of Chemistry Humboldt Universitat zu Berlin12489 BerlinGermany Institute of Agronomy and Crop Physiology Justus-Liebig University Giessen35392 GiessenGermany department of Physiology&Cell Biology Leibniz-lnstitute of Plant Genetics and Crop Plant Research06466 GaterslebenGermany

In plants, phosphate (Pi) homeostasis is regulated by the interaction of PHR transcription factors with stand-alone SPX proteins, which act as sensors for inositol pyrophosphates. Here, we combined different methods to obtain a comprehensive picture of how inositol (pyro)phosphate metabolism is regulated by Pi and dependent on the inositol phosphate kinase ITPK1. We found that inositol pyrophosphates are more responsive to Pi than lower inositol phosphates, a response conserved across kingdoms. With CE-ESI-MS we could separate different InsP7 isomers in Arabidopsis and rice, and identify 4/6-InsP7 and a PP-InsP4 isomer hitherto not reported in plants. We found that the inositol pyrophosphates 1/3-InsP7, 5-InsP7 and InsP8 increase severalfold in shoots after Pi resupply and that tissue-specific accumulation of inositol pyrophosphates relies on ITPK1 activities and MRP5-dependent InsP6 compartmentalization. Notably, ITPK1 is critical for Pi-dependent 5-InsP7 and InsP8 synthesis in planta and its activity regulates Pi starvation responses in a PHR-dependent manner. Furthermore, we demonstrate that ITPK1-mediated conversion of InsP6 to 5-InsP7 requires high ATP concentrations and that Arabidopsis ITPK1 has an AdP phosphotransferase activity to dephosphorylate specifically 5-InsP7 under low ATP. Collectively, our study provides deeper insights into Pi-dependent changes in nutritional and energetic states with the synthesis of regulatory inositol pyrophosphates.

关键词： diphosphoinositol pentakisphosphate kinase inositol 1,3,4-trisphosphate 5/6-kinase 1 inositol phosphates inositol pyrophosphates phosphate homeostasis phosphate signaling

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急性缺血性卒中的血管内治疗：神经介入外科学学会实践标准委员会的报告

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国际脑血管病杂志 2012年第12期20卷 905-912页

作者： K A Blackham P M Meyers T A Abruzzo F C Alberquerque d Fiorella J Fraser d.frei C d Gandhi d V Heck J A Hirsch d P Hsu M Jayaraman S Narayanan C Prestigiacomo J L Sunshine 包元飞(译) 李永坤(译) 刘新峰(译) 代表神经介入外科学学会南京大学医学院临床学院南京军区南京总医院神经内科 210002

目的总结采用血管内技术治疗急性缺血性卒中的有关证据并等级分类。方法对美国心脏协会（American Heart Association，AHA）以前发表的推荐意见进行审查并作为当前流程的基础。在此这基础上，进行系统的文献回顾以评估支持急性缺血性... 详细信息

目的总结采用血管内技术治疗急性缺血性卒中的有关证据并等级分类。方法对美国心脏协会（American Heart Association，AHA）以前发表的推荐意见进行审查并作为当前流程的基础。在此这基础上，进行系统的文献回顾以评估支持急性缺血性卒中血管内治疗的证据。根据AHA卒中委员会和牛津大学循证医学中心提出的循证医学证据分级指南进行评估。对操作安全性、技术效果以及对临床转归的影响进行特别审核。

关键词：急性缺血性卒中血管内治疗委员会神经介入外科学标准学会美国心脏协会

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中元古代具有高的Cr同位素分异特征和高的大气氧浓度

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矿物岩石地球化学通报 2018年第5期37卷 902-902页

作者：袁超 Canfield d E Zhang S C Frank A B Wang X M Wang H J Su J Ye Y T frei R.

中元古代的大气氧浓度一直都不确定,然而该时期大气氧浓度对真核生物的爆发生长却具有重要意义。前人通过沉积物中的Cr同位素研究,认为中元古代具有非常低的大气氧浓度(当今氧水平的0.1%),并不足以使得中元古代真核生物生长以及后期新... 详细信息

中元古代的大气氧浓度一直都不确定,然而该时期大气氧浓度对真核生物的爆发生长却具有重要意义。前人通过沉积物中的Cr同位素研究,认为中元古代具有非常低的大气氧浓度(当今氧水平的0.1%),并不足以使得中元古代真核生物生长以及后期新元古代冠群生物的演化。

关键词：中元古代氧浓度同位素大气 Cr 特征和分异真核生物

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