检索结果-南通市图书馆

Nano Research 2021年第11期14卷 4063-4072页

作者： anchun Tang Chubin Wan Xiaoyu Hu Xin Ju School of Mathematics and Physics University of Science and Technology BeijingBeijing100083China

Ni/ZnO nano-sponges have been successfully synthesized through optimized annealing of Ni/Zn-based organic framework (Ni/Zn-MOF). The annealed MOF provides the stable carbon structure with 3d interconnection and prevents structural collapse during the charging and discharging process. The annealing causes the incorporation of intrinsic Ni^(3+) in the surface of NiO nanoparticles, providing more reaction active sites. The oxygen vacancies in ZnO and heterostructure interfaces between NiO and ZnO promote the charge transformation. Based on the aforementioned advantages, the Ni/ZnO nanocomposites exhibit excellent electrocatalytic performances for supercapacitors. The specific capacitance can reach to 807 F·g^(−1) at 1 A·g^(−1) in the studied electrodes. After 5,000 cycles at 10 A·g^(−1), the cyclic stability remains excellent at 86% of the initial capacitance. Moreover, the as-prepared asymmetric supercapacitor exhibits a high energy density of 30.6 W·h·kg^(−1) at power density of 398 W·kg^(−1). This study is expected to provide new insights into exploring the potential mechanism of catalyst action.

关键词： metal-organic frameworks ZnO-based nano-sponges surface vacancies supercapacitor electrochemical performance

Reproductive toxicity study with a novel deoxyguanosine analogue （Metacavir） in pregnant Sd rats

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2015年第1期9卷 82-89页

作者： Qihui Luo Zhengli Chen anchun Cheng Mingshu wang Jing Fang Xi Peng Li Tang Key Laboratory of Animal disease and Human Health of Sichuan Province Ya 'an 625014 China Institute of Preventive Veterinary Medicine Sichuan Agricultural University Chengdu 611130 China

Our preliminary studies demonstrated that Metacavir has potential to become a new anti-HBV agent. The main targets of the toxic effects of Metacavir, in rhesus monkeys, were gastrointestinal tracts, liver, blood, and kidneys, which were not related to mitochondrial effects. In this study, the maternal toxicity, embryo-fetal developmental toxicity and teratogenicity were studied in pregnant Sprague-dawley rats after intragastric administration of Metacavir （200, 100, 50, 0 mg/kg body weight） during the first 6-15 days of pregnancy. Slower weight gain was observed in 5 out of 21 rats subjected to a 200 mg/kg dose, as well as 2 out of 20 subjected to a 100 mg/kg dose. Compared with the solvent control group, the calibration weight gain in the 200 mg/kg and 100 mg/kg dosage groups respectively, during first 6-20 pregnant days were significantly different （P 〈 0.01, P 〈 0.05）. Significant dose related adverse effects to other reproductive parameters were not seen in F0 and F1, but the number of stillbirths in high dose group showed notably difference compared with the control group （P 〈 0.05）, while the litter incidence showed no difference. No Metacavir-associated pathological changes were observed. The present research indicated that at a dose of 200 mg/（kg·d）（i.e., 40 times the effective dose in rats）, Metacavir shows some maternal toxicity to Sd rats. The embryotoxicity in the 200 mg/kg group encompass decreased fetal body weight, and higher fetal mortality rates, compared with the control group. However, the litter incidence showed no statistical difference. All the treated rats displayed normal bone development, no teratogenicity and without adverse effects on fetal development, thus indicating that below a dose of 200 mg/（kg· d） there is no teratogenic side effects.

关键词： deoxyguanosine analogue Metacavir pregnancy maternal toxicity embryo toxicity teratogenicity

雏鹅新型病毒性肠炎病毒的分离、鉴定及病原特性的研究

在线全文

学校读者我要写书评

暂无评论

畜牧兽医学报 2000年第6期31卷 548-556页

作者：程安春四川省动物生物技术重点实验室雅安625014

利用原代鸭胚成纤维细胞培养及蚀斑克隆技术从具有典型渗出性、坏死性病变的雏鹅新型病毒性肠炎 (NGVE)病例的实质器官和肠道内容物中分离到 2 6株病毒。经鉴定 ,2 6株病毒的中和性抗原是一致的。病毒 (NGVEV)呈球形或椭圆形 ,无囊膜 ,... 详细信息

利用原代鸭胚成纤维细胞培养及蚀斑克隆技术从具有典型渗出性、坏死性病变的雏鹅新型病毒性肠炎 (NGVE)病例的实质器官和肠道内容物中分离到 2 6株病毒。经鉴定 ,2 6株病毒的中和性抗原是一致的。病毒 (NGVEV)呈球形或椭圆形 ,无囊膜 ,直径 70～ 90nm。不凝集鸡、鸭、鹅、鸽、黄牛、水牛及猪的红细胞。对氯仿处理 1～ 3次不敏感。病毒于 - 15℃和 0℃至少可以分别保存 36和 2 0个月 ;于 37℃ 4 5d、4 5℃ 4 8h、56℃ 5h、60℃ 1h不影响病毒对细胞的致病变能力和对雏鹅的致病性 ;于 80℃ 5min和煮沸 ( 96℃ ) 10s可以使病毒失活。病毒经药物抑制法和酶消化法均鉴定其核酸类型为dNA。在CsCl中浮密度为 1 32 g/cm3。pH1 0和pH10 0处理 1h可使病毒失活 pH2 0和 9 0可使病毒滴度有所下降 ,pH3 0～ 8 0对病毒的感染性没有影响。NGVEV与鸭瘟病毒和小鹅瘟病毒没有中和抗原相关性。分离的病毒通过滴鼻、点眼、皮下注射、肌肉注射、口服等途经人工感染均可复制出与临床表现一致的病例 ,其中口服是最佳感染途径。根据分离到的病毒特性。

关键词：雏鹅新型病毒性肠炎腺病毒分离鉴定

Preparation of multigradient hydroxyapatite scaffolds and evaluation of their osteoinduction properties

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2022年第1期9卷 189-201页

作者： Hao Huang anchun Yang Jinsheng Li Tong Sun Shangke Yu Xiong Lu Tailin Guo Ke duan Pengfei Zheng Jie Weng Key Laboratory of Advanced Technologies of Materials(MOE) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031P.R.China Engineering Laboratory of Orthopedic Implant device development and Application Technology Clinical College of MedicineSouthwest Medical UniversityLuzhou 646000P.R.China department of Orthopaedic surgery Children’s Hospital of Nanjing Medical UniversityNanjing 210008P.R.China

Porous hydroxyapatite(HA)scaffolds are often used as bone repair materials,owing to their good biocompatibility,osteoconductivity and low *** and osteoinductivity of porous HA scaffolds were limited in clinical application,and these disadvantages were need to be improved *** used water-in-oil gelation and pore former methods to prepare HA spheres and a porous cylindrical HA container,*** prepared HA spheres were filled in container to assemble into composite *** adjusting the solid content of the slurry(solid mixture of chitin sol and HA powder)and the sintering temperature,the porosity and crystallinity of the HA spheres could be significantly improved;and mineralization of the HA spheres significantly improved the biological activity of the composite *** multigradient(porosity,crystallinity and mineralization)scaffold(HA-700)filled with the mineralized HA spheres exhibited a lower compressive strength;however,in vivo results showed that their vascularization ability were higher than those of other groups,and their osteogenic Gini index(Go:an index of bone mass,and inversely proportional to bone mass)showed a continuous decrease with the implantation *** study provides a new method to improve porous HA scaffolds and meet the demands of bone tissue engineering applications.

关键词： porous hydroxyapatite scaffold biocompatibility osteoinduction bone tissue engineering

末次冰消期冲绳海槽中部表层和温跃层海水温度演化的脱耦现象

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

地球科学 2022年第7期47卷 2602-2615页

作者：钱芳常凤鸣孙晗杰李铁刚李安春王佳中国科学院海洋研究所海洋地质与环境重点实验室山东青岛266071 青岛海洋科学与技术试点国家实验室海洋地质过程与环境功能实验室山东青岛266237 中国科学院大学北京100049 自然资源部第一海洋研究所海洋沉积与环境地质重点实验室山东青岛266061 中国科学院海洋大科学研究中心山东青岛266071

解读末次冰消期全球水文气候演变过程对于理解气候系统对内外强迫的响应具有重要意义.以冲绳海槽中部OKI02岩心为材料,通过浮游有孔虫Globigerinodes ruber和Pulleniatina obliquiloculata壳体Mg/Ca比值分别重建了19 ka BP以来海槽中部... 详细信息

解读末次冰消期全球水文气候演变过程对于理解气候系统对内外强迫的响应具有重要意义.以冲绳海槽中部OKI02岩心为材料,通过浮游有孔虫Globigerinodes ruber和Pulleniatina obliquiloculata壳体Mg/Ca比值分别重建了19 ka BP以来海槽中部表层和温跃层海水温度(SST和TWT),结合浮游有孔虫群落组成变化重点恢复了末次冰消期(~18~11.7 ka BP)上层水体温度变化的特征和过程.结果显示SST在LGM显著偏低,末次冰消期表现为显著的千年尺度变化,清楚地记录了HS1、B/A、Yd等快速气候波动事件. 19 ka BP以来重建的TWT整体呈明显的上升趋势,但波动频繁而剧烈,末次冰消期相对较低,未显示显著的千年尺度变化.对比北半球高纬和热带太平洋的记录发现,末次冰消期冲绳海槽中部SST开始上升的时间基本与前者相当,但明显滞后于热带西太平洋;冰消期其变化模式明显区别于热带西太平洋持续稳定的升温过程,而更类似于北半球高纬区的变化.与SST明显不同,海槽区温跃层的升温(~18 ka BP)明显早于北半球高纬变暖,却接近于热带西太平洋海表温度开始上升的时间;且TWT的上升和波动方式也更接近于热带太平洋海温的变化模式.对末次冰消期SST和TWT差异化演变的分析表明,AMOC对中低纬大气环流的影响可能通过东亚冬季风强度的变化控制了海槽区SST的演变,而热带太平洋ENSO过程则可能通过黑潮强度的变化决定了区域TWT的演化.末次冰消期冲绳海槽中部SST和TWT演化存在明显的脱耦现象,显示了其与高、低纬海洋和气候变化之间的密切联系.

关键词：冲绳海槽末次冰消期表层及温跃层温度黑潮东亚冬季风 ENSO 海洋地质学

3种工艺制作全口义齿基托精确度与固位力的比较

在线全文

学校读者我要写书评

暂无评论

中华口腔医学杂志 2022年第9期57卷 927-931页

作者：李丹杨胜涛袁泉莫安春岳莉四川大学华西口腔医院种植科口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心成都610041

目的比较传统注塑与两种数字化制作工艺(切削和三维打印)制作的全口义齿基托精确度与固位力,以期为临床提供参考。方法使用商用成品无牙上颌模型翻制石膏工作模型10个,进行编号并扫描获取数据。对各工作模型同时设计数字化基托蜡型并制... 详细信息

目的比较传统注塑与两种数字化制作工艺(切削和三维打印)制作的全口义齿基托精确度与固位力,以期为临床提供参考。方法使用商用成品无牙上颌模型翻制石膏工作模型10个,进行编号并扫描获取数据。对各工作模型同时设计数字化基托蜡型并制作实体基托蜡型,分别使用注塑、切削、三维打印3种制作工艺制作3组全口义齿基托(每组各10个)。扫描获取3组基托组织面数据。分析各工作模型组织面与3组基托组织面的数据偏差(即精确度,数值越小精确度越高)。使用万能力学测试机测试3组基托固位力并比较。结果切削组精确度[(0.076±0.026)mm]显著高于注塑组[(0.120±0.025)mm]和三维打印组[(0.117±0.041)mm](P0.05)。切削组固位力[(9.55±2.44)N]显著大于三维打印组[(5.19±0.06)N](P<0.05),三维打印组固位力显著大于注塑组[(1.52±0.52)N](P<0.05)。结论切削工艺制作的全口义齿基托的精确度和固位力最佳,三维打印工艺制作的全口义齿基托仅固位力优于注塑工艺,两种数字化制作工艺均可满足临床使用要求。

关键词：义齿基托义齿,全口,上颌计算机辅助设计义齿固位工艺学,牙科三维打印

development and Application of a One-step Real-time TaqMan RT-PCR Assay to detect duck Hepatitis Virus Type 1

在线全文

学校读者我要写书评

暂无评论

Development and Application of a One-step Real-time TaqMan R...

Isotopic feature and uranium dating of the volcanic rocks in the Okinawa Trough

第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： Miao Yang anchun Cheng Mingshu wang (Avian disease Research Center,College of Veterinary Medicine,Sichuan Agricultural University,Yaan 625014,PR China The Key Laboratory of Animal disease and Human Health of Sichuan Province,Yaan 625014,PR China)

A one-step real-time reverse-transcription polymerase chain reaction assay (rRT-PCR) was developed for efficient detection of duck hepatitis virus type 1 (dHV-1).A pair of specific primers which showed 87bp fragment was designed against the conserved region in the 3d gene with a single conserved TaqManTM *** could obtain excellent linear when the dHV-1 RNA concentration between 1×10 and 1×10 *** detection limit of this assay was 10 viral genomic copies per reaction and it was highly specific to dHV-1 ***,the rRT-PCR assay was used to determine the distribution and concentration of dHV-1 virulent strain in duck embryos as well as the dHV-1 less-virulent strain in chicken *** results revealed that the copy number of dHV-1 reached a peak in duck embryos and chicken embryos at 28-40h,44-56h post inoculation respectively,maintaining 10 copies/g level in embryoid bodies and 10-10 copies/ml level in allantoic *** RNA copy number in chorioallantoic membrance and muscle was higher than that in allantoic fluid,heart,liver,spleen,brain,intestine from dead duck and chicken embryos inoculated with *** this method and neutralization test to detect 10 liver samples taking from ducklings after artificially infected and 40 clinical liver samples taking from suspected ducklings showed that the positive results of artificially infected samples were the same,while the rRT-PCR method was more sensitive than neutralization test for clinical samples detection,and the positive rate was significant difference (Pd,sensitive, specific,reliable and *** will be a powerful tool for dHV-1 suspected case detection,distribution pattern of dHV-1 in vivo and molecular epidemiological screening.

关键词： duck hepatitis virus type1 rRT-PCR duck embryo Chicken embryo distribution

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国科学：地球科学英文版 2006年第4期49卷 375-383页

Volcanic rocks from the northern and middle Okinawa Trough were dated by ura- nium-series dating method. differential fractions using magnetic procedure were designed to separate samples. New report on the ages and isotopic data of rocks in the northern trough (especially black pumice) was discussed. Based on the uranium dates and Sr-Nd isotopic ratio, magmatic evolution process of the Okinawa Trough was noted. Firstly, there have been wide silicic volcanic activities in the Okinawa Trough from late Pleistocene to present, and the volcanic rocks can be divided into three subgroups. Secondly, magma generally came from PREMA source area under the Okinawa Trough. Magmatic evolution in the northern trough was similar to the middle, but different to the south. Finally, volcanic activities indicated that opening of the southern Okinawa Trough did not happen due to the collision between Luson Arc and Eurasian Plate until the early Pleistocene.

关键词： Okinawa Trough, black pumice, uranium-series dating, Sr-Nd isotopic ratio, magmatic evolution.

雏鸭人工感染黄曲霉毒素及其毒素含量的动态变化

在线全文

学校读者我要写书评

暂无评论

中国家禽 2007年第17期29卷 8-10页

作者：刘艳丽程安春汪铭书胡骑陈孝跃四川农业大学动物医学院禽病防治研究中心

通过7日龄樱桃谷鸭饲喂黄曲霉毒素(AFB1)含量为150bbp的全价饲料,复制雏鸭感染黄曲霉毒素急性中毒病例,观察中毒鸭的临床症状;于6、12、24、48、72、96、120、144、168和192h分别剖杀2只,观察大体病理变化,并用ELISA试剂盒测定血液、肝... 详细信息

通过7日龄樱桃谷鸭饲喂黄曲霉毒素(AFB1)含量为150bbp的全价饲料,复制雏鸭感染黄曲霉毒素急性中毒病例,观察中毒鸭的临床症状;于6、12、24、48、72、96、120、144、168和192h分别剖杀2只,观察大体病理变化,并用ELISA试剂盒测定血液、肝脏中的AFB1含量。黄曲霉毒素急性中毒病例的临床症状主要为:生长停滞,跛行,运动失调,死前呈角弓反张等神经症状。剖检病理变化主要为:气囊混浊有黄色纤维素样渗出物,肝脏肿大呈土黄色,质地变脆并有出血点。饲喂黄曲霉毒素饲料6h后即可从血液、肝脏中检出AFB1,随着饲喂黄曲霉毒素饲料时间的延长,血液、肝脏中AFB1的含量逐渐增高,7d时肝脏和血清中的AFB1分别高达53.4ng/g和35.68ng/g。

关键词：黄曲霉毒素雏鸭 ELISA

下调TPX2抑制喉癌细胞生长的体外实验研究

在线全文

学校读者我要写书评

暂无评论

现代肿瘤医学 2019年第8期27卷 1314-1319页

作者：周雪琴王敏王刘倩赵力邓安春重庆市陆军军医大学新桥医院耳鼻喉科重庆400037

目的:研究TPX2对喉癌细胞增殖、克隆形成能力及细胞周期的影响。方法:Hep-2细胞中转染TPX2 siRNA、siRNA control记为TPX2 siRNA、siRNA-NC组,以不做转染的细胞为Con组。荧光定量PCR和Western blot分别测定细胞中TPX2 mRNA和蛋白水平,MT... 详细信息

目的:研究TPX2对喉癌细胞增殖、克隆形成能力及细胞周期的影响。方法:Hep-2细胞中转染TPX2 siRNA、siRNA control记为TPX2 siRNA、siRNA-NC组,以不做转染的细胞为Con组。荧光定量PCR和Western blot分别测定细胞中TPX2 mRNA和蛋白水平,MTT测定各组细胞增殖,平板克隆实验测定各组细胞克隆形成能力,流式细胞术测定各组细胞周期情况,Western blot测定各组细胞中增殖细胞核抗原(PCNA)、细胞核增殖抗原(Ki-67)、细胞周期依赖性蛋白激酶4(CdK4)、细胞周期蛋白d1(Cyclin d1)蛋白水平。结果:siRNA control中TPX2 mRNA和蛋白水平、细胞存活率、克隆形成数目、细胞周期以及细胞中PCNA、Ki-67、CdK4、Cyclin d1蛋白水平与Con相比均没有明显变化(P>0.05)。TPX2 siRNA细胞中TPX2 mRNA和蛋白水平均明显低于Con(PdK4、Cyclin d1蛋白水平明显低于Con(PdK4、Cyclin d1蛋白表达。

关键词：喉癌增殖凋亡细胞周期 TPX2