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Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences

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Plant Communications 2021年第2期2卷 78-88页

作者： Simon Sretenovic desuo Yin Adam Levav Jeremy d.Selengut Stephen M.Mount Yiping Qi department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA Hubei Academy of Agricultural Sciences Wuhan 430064China Montgomery Blair High School Silver SpringMD 20901USA Center for Bioinformatics and Computational Biology University of MarylandCollege ParkMD 20742USA department of Cell Biology and Molecular Genetics University of MarylandCollege ParkMD 20742USA Institute for Bioscience and Biotechnology Research University of MarylandRockvilleMD 20850USA

The most popular CRISPR-SpCas9 systemrecognizes canonical NGG protospacer adjacent motifs(PAMs).Previously engineered SpCas9 variants,such as Cas9-NG,favor G-rich PAMs in genome *** this manuscript,we describe a new plant genome-editing system based on a hybrid iSpyMacCas9 platform that allows for targeted mutagenesis,C to T base editing,and A to G base editing at A-rich *** study fills amajor technology gap in the CRISPR-Cas9 system for editing NAAR PAMs in plants,which greatly expands the targeting scope of ***,our vector systems are fully compatible with Gateway cloning and will work with all existing single-guide RNA expression systems,facilitating easy adoption of the systems by *** anticipate that more tools,such as prime editing,homology-directed repair,CRISPR interference,and CRISPR activation,will be further developed based on our promising iSpyMac-Cas9 platform.

关键词： plant genome editing iSpyMacCas9 PAM cytosine base editing adenine base editing

Effects of stenoses on non-Newtonian flow of blood in blood vessels

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International Journal of Biomathematics 2015年第1期8卷 123-135页

作者： Om Prakash O. d. Makinde S. P. Singh Nidhi Jain devendra Kumar School of Basic and Applied Sciences Galgotias University Greater Noida Uttar Pradesh India Faculty of Military Science Stellenbosch University Private Bag X2 Saldanha 7395 South Africa department of Mathematics DEI Dayalbagh Agra Uttar Pradesh India department of Mathematics Sachdeva Institute of Technology Farah Mathura Uttar Pradesh India

In this paper, a mathematical model for steady blood flow through blood vessels with uniform cross-section in stenoses arteries has been proposed. Blood is assumed to be non- Newtonian, incompressible and homogeneous fluid. Blood in human artery is represented as Bingham plastic fluid. Expressions for flow rate, wall shear stress, and resistance to flow against stenoses size have been obtained. Obtained results indicate that stenoses size decreases the flow rate and increases the wall shear stress as well as resistance to flow.

关键词： Stenoses wall shear stress resistance to flow flow rate non-Newtonian fluid

Online evaluation of electroless deposition rate by electrochemical noise method

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Transactions of Nonferrous Metals Society of China 2019年第8期29卷 1753-1762页

作者： Z.RAJABALIZAdEH d.SEIFZAdEH A.HABIBI-YANGJEH Applied Chemistry department Faculty of Basic Science University of Mohaghegh Ardabili

Continuous noise resistance calculation(CNRC)technique was used for online determination of the electroless nickel deposition rate on zirconium pretreated magnesium *** this purpose,the noise resistance(R_n) variation with time was calculated for the pretreated alloy surface in the electroless plating *** CNRC results were described by energy dispersive X-ray spectroscopy(EdS)and scanning electron microscopy(SEM)***,potentiodynamic polarization and gravimetric measurements were used for determination of the electroless deposition rate at the same time period and the results were compared with the CNRC *** Rn variation with plating time shows that the electroless plating consists of different stages with various deposition *** results of the CNRC and polarization methods were not in acceptable agreement due to the limitations of the polarization method for online monitoring of the deposition ***,the results of the gravimetric measurements were in complete agreement with the CNRC technique and so,the CNRC can be considered as suitable tool for online evaluation of the electroless deposition rate.

关键词： magnesium alloy electroless plating deposition rate electrochemical noise SEM EdS

Statistical Model for Impact and Energy Absorption of 3d Printed Coconut Wood-PLA

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Energy Engineering 2021年第5期118卷 1305-1315页

作者： J.Kananathan M.Samykano K.Kadirgama d.Ramasamy M.M.Rahman Green Kingdom Solutions Sdn.Bhd. Taman Salak SelatanKuala Lumpur57100Malaysia College of Engineering Universiti Malaysia PahangGambangPahang26300Malaysia Faculty of Mechanical&Automotive Engineering Technology Universiti Malaysia PahangPekanPahang26600Malaysia

Fused deposition modeling(FdM)-3d printing has been the favored technology to build functional components in various *** present study investigates infill percentage and infill pattern effects on the printed parts’impact properties through the 3d printing technique using coconut wood-filled PLA *** models are also proposed in the present study with the aim for future property *** to the ASTM standard,fifteen specimens with different parameter combinations were printed using a low-cost FdM 3d printer to evaluate their impact *** analysis was performed using MINITAB to validate the experimental data and model *** experimental outcomes reveal the honeycomb pattern with 75%infill density achieves the highest energy absorption(0.837 J)and impact energy(5.1894 kJ/m^(2)).The p-value from statistical analysis clearly shows that all the impact properties are less than the alpha value of 0.05,suggesting all the properties are vital to determine the impact *** validation process affirms that the generated mathematical model for the energy absorbed and the impact energy is reliable at an acceptable level to predict their respective *** errors between the experimental value and the predicted value are 3.98%for the energy absorbed and 4.06%for impact *** findings are expected to provide insights on the impact behavior of the coconut wood-filled PLA composites prepared by FdM-3d printing and a mathematical model to predict the impact properties.

关键词： Impact energy energy absorption response surface methodology coconut wood ANOVA

An antimicrobial peptide from endophytic Fusarium tricinctum of Rhododendron tomentosum Harmaja

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Fungal diversity 2013年第3期60卷 153-159页

作者： M.V.Tejesvi d.R.Segura K.M.Schnorr d.Sandvang S.Mattila P.B.Olsen S.Neve T.Kruse H.H.Kristensen A.M.Pirttilä departments of Biology University of OuluP.O.Box 3000LinnanmaaFIN-90014 OuluFinland Novozymes A/S Krogshoejvej 362880 BagsvaerdDenmark departments of Chemistry University of OuluP.O.Box 3000LinnanmaaFIN-90014 OuluFinland

Endophytic microbes have attracted considerable attention as a completely new source of pharmaceuticals in recent *** endophytic Fusarium tricinctum was isolated from Rhododendron tomentosum,a shrub that is found widely across the northern *** endophytic *** produced antimicrobial compounds that were active against Staphylococcus carnosus,Candida albicans and *** transcriptome of the *** was sequenced,and a total of 12,006 contigs were assembled,having an N50 value of 1390 *** of the transcriptomic Pd library of *** yielded an antimicrobial peptide named *** expression of Trtesin transcripts was>1000 fold in the mRNA library originating from PdB-grown fungi exhibiting high antimicrobial activity compared to FG4-grown fungi with no antimicrobial *** was cloned,expressed,and purified in pET32(a),and it consisted of 52 amino acids with 6 cysteine *** molecular weight of Trtesin is 6138.92 *** additional N-terminal sequencing was done to confirm the intact peptide,as well as to check the correct amino acid *** MIC of Trtesin was determined against several bacteria as 64μg/mL,and peptide showed a mild activity against *** in agar diffusion assay,as a zone of inhibition of 10 mm was formed at 100μg of peptide.

关键词： Fungal defensin Antimicrobial Endophyte Transcriptome

Enabling Virtual Met Masts for wind energy applications through machinelearning-methods

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Energy and AI 2023年第1期11卷 26-42页

作者： Sandra Schwegmann Janosch Faulhaber Sebastian Pfaffel Zhongjie Yu Martin dörenkämper Kristian Kersting Julia Gottschall Fraunhofer IWES Küpkersweg 7026129 OldenburgGermany Fraunhofer IEE Königstor 5934119 KasselGermany Technical University of darmstadt Karolinenplatz 564289 DarmstadtGermany Hessian Center for AI(hessian.AI) Mornewegstraße 3064293 DarmstadtGermany

As wind is the basis of all wind energy projects, a precise knowledge about its availability is needed. For ananalysis of the site-specific wind conditions, Virtual Meteorological Masts (VMMs) are frequently used. VMMsmake use of site calibrated numerical data to provide precise wind estimates during all phases of a wind energyproject. Typically, numerical data are used for the long-term correlation that is required for estimating theyield of new wind farm projects. However, VMMs can also be used to fill data gaps or during the operationalphase as an additional reference data set to detect degrading sensors. The value of a VMM directly dependson its ability and precision to reproduce site-specific environmental conditions. Commonly, linear regressionis used as state of the art to correct reference data to the site-specific conditions. In this study, a frameworkof 10 different machine-learning methods is tested to investigated the benefit of more advanced methods ontwo offshore and one onshore site. We find significantly improving correlations between the VMMs and the reference data when using more advanced methods and present the most promising ones. The K-NearestNeighbors and AdaBoost regressors show the best results in our study, but Multi-Output Mixture of GaussianProcesses is also very promising. The use of more advanced regression models lead to decreased uncertainties;hence those methods should find its way into industrial applications. The recommended regression models canserve as a starting point for the development of end-user applications and services.

关键词： Virtual Met Mast(VMM) Wind power Machine learning Reanalysis data Site assessment Weather Research and Forecasting Model(WRF) Measure-Correlate-Predict(MCP)

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气垫车辆土壤参数估值算法

上海交通大学学报 2011年第4期45卷 451-456页

作者：许烁喻凡罗哲 Pham d T 纪赜上海交通大学机械与动力工程学院上海200240 卡迪夫大学制造工程中心

针对气垫车辆具有附加的垂向力控制自由度的特点,提出了基于g函数和扩展卡尔曼滤波器(EKF)联合算法的土壤参数估值方案.g函数通过解耦土壤参数解决了多解问题,EKF通过减小测量不确定度提高了估值准确性.通过不同噪声水平下算法准确性和... 详细信息

针对气垫车辆具有附加的垂向力控制自由度的特点,提出了基于g函数和扩展卡尔曼滤波器(EKF)联合算法的土壤参数估值方案.g函数通过解耦土壤参数解决了多解问题,EKF通过减小测量不确定度提高了估值准确性.通过不同噪声水平下算法准确性和大噪声重复实验下算法稳定性的实验表明:g-EKF联合算法在各种噪声水平下具有应用鲁棒性,特别是在大噪声工况下,其估值准确性、精确性和稳定性具有明显优势.

关键词：土壤参数估值 g—EKF算法气垫车状态噪声测量噪声

Negative capsule endoscopy in patients with obscure gastrointestinal bleeding reliable: Recurrence of bleeding on long-term follow-up

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World Journal of Gastroenterology 2013年第28期19卷 4520-4525页

作者： Maria Elena Riccioni Riccardo Urgesi Rossella Cianci Gianluca Rizzo Luca d'Angelo Riccardo Marmo Guido Costamagna digestive Endoscopy Unit Catholic University of Rome 00168 Rome Italy Gastroenterology and digestive Endoscopy Unit Ospedale Belcolle 01100 Viterbo Italy department of Internal Medicine Catholic University of Rome 00168 Rome Italy department of Surgery Catholic University of Rome 00168 Rome Italy department of Neurosurgery Ospedale Belcolle 01100 Viterbo Italy Gastroenterology Unit Ospedale di Polla 84035 Polla Salerno Italy

AIM: To assess the rate of recurrent bleeding of the small bowel in patients with obscure bleeding already undergone capsule endoscopy (CE) with negative results. METHOdS: We reviewed the medical records related to 696 consecutive CE performed from december 2002 to January 2011, focusing our attention on patients with recurrence of obscure bleeding and negative CE. Evaluating the patient follow-up, we analyzed the recurrence rate of obscure bleeding in patient with a negative CE. Actuarial rates of rebleeding during follow-up were calculated, and factors associated with rebleeding were assessed through an univariate and multivariate analysis. A P value of less than 0.05 was regarded as statistically significant. The sensitivity, specificity, and positive and negative predictive values (PPV and NPV) of negative CE were calculated. RESULTS: Two hundred and seven out of 696 (29.7%) CE studies resulted negative in patient with obscure/overt gastrointestinal bleeding. Overall, 489 CE (70.2%) were positive studies. The median follow-up was 24 mo (range 12-36 mo). during follow-up, recurrence of obscure bleeding was observed only in 34 out of 207 negative CE patients (16.4%); 26 out of 34 with obscure overt bleeding and 8 out of 34 with obscure occult bleeding. The younger age (d the onset of bleeding such as melena are independent risk factors of rebleeding after a negative CE (OR = 2.6703, 95%CI: 1.1651-6.1202, P = 0.0203; OR 4.7718, 95%CI: 1.9739-11.5350, P = 0.0005). The rebleeding rate (CE+ vs CE-) was 16.4% vs 45.1% (χ 2 test, P = 0.00001). The sensitivity, specificity, and PPV and NPV were 93.8%, 100%, 100%, 80.1%, respectively. CONCLUSION: Patients with obscure gastrointestinal bleeding and negative CE had a significantly lower rebleeding rate, and further invasive investigations can be deferred.

关键词： Capsule endoscopy Enteroscopy Anemia Obscure gastrointestinal bleeding Rebleeding

Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L.

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Horticulture Research 2019年第1期6卷 1560-1570页

作者： Tamara d.Collum Elizabeth Lutton C.douglas Raines Christopher dardick James N.Culver Institute for Bioscience and Biotechnology Research College ParkMDUSA USdA-ARS Appalachian Fruit Research LaboratoryKearneysvilleWVUSA department of Plant Science and Landscape Architecture University of MarylandCollege ParkMDUSA

Phloem plays a fundamental role in plants by transporting hormones,nutrients,proteins,RNAs,and carbohydrates essential for plant growth and ***,the identity of the underlying phloem genes and pathways remain enigmatic especially in agriculturally important perennial crops,in part,due to the technical difficulty of phloem ***,we used two phloem-specific promoters and a translating ribosome affinity purification(TRAP)strategy to characterize the phloem translatome during leaf development at 2,4,and 6 weeks post vernalization in plum(Prunus domestica L.).Results provide insight into the changing phloem processes that occur during leaf *** processes included the early activation of dNA replication genes that are likely involved in phloem cell division during leaf expansion,as well as the upregulation of phloem genes associated with sink to source conversion,induction of defense processes,and signaling for *** these results reveal the dynamics of phloem gene expression during leaf development and establish the TRAP system as a powerful tool for studying phloem-specific functions and responses in trees.

关键词： alterations crops likely