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kisspeptin调控Notch1/Akt/Foxo1通路参与复发性流产患者子宫内膜蜕膜化

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四川大学学报（医学版） 2024年第3期55卷 542-551页

作者：杨艳红张建亮李冬晓刘翠平郭融肖伊周玲佟玲霞张弘苏州大学附属第二医院妇产科苏州215004 苏州大学附属第一医院江苏省临床免疫研究所苏州215021

目的 kisspeptin是一种肿瘤抑制因子,由KISS1基因编码。Notch1/Akt/Foxo1是调节细胞增殖、分化的重要信号通路。本研究探讨kisspeptin通过Notch1/Akt/Foxo1信号通路调控子宫内膜蜕膜化在复发性流产(RSA)中的作用机制。方法纳入2020年6... 详细信息

目的 kisspeptin是一种肿瘤抑制因子,由KISS1基因编码。Notch1/Akt/Foxo1是调节细胞增殖、分化的重要信号通路。本研究探讨kisspeptin通过Notch1/Akt/Foxo1信号通路调控子宫内膜蜕膜化在复发性流产(RSA)中的作用机制。方法纳入2020年6月–2020年12月苏州大学附属第二医院生殖中心门诊收治的RSA患者45例及同期计划生育门诊择期人工流产女性50例,Western blot、RT-qPCR检测蜕膜组织中kisspeptin(及其基因KISS1)、蜕膜化标志物胰岛素样生长因子结合蛋白1(insulin-like growth factor binding protein 1, IGFBP1)、Notch1、Akt、Foxo1表达水平。使用siRNA干扰KISS1或添加kisspeptin10(kisspeptin的活性片段)处理永生化子宫内膜基质细胞(hESC)后,命名为siKP组或KP10组,并设转染空白siRNA的hESC为对照组,CCK8检测增殖情况。对3组hESC进行体外诱导蜕膜化,免疫荧光检测siKP组和KP10组细胞Notch1表达及蜕膜化形态差异,RT-qPCR和Western blot检测3组细胞IGFBP1、Notch1、Akt、Foxo1的表达。hESC添加Notch1、Akt、Foxo1对应抑制剂后诱导蜕膜化(以仅诱导蜕膜化、未添加抑制剂的hESC为正常对照组),检测4组细胞上述蛋白和基因的表达。建立正常妊娠(CBA/J×BALB/c)和复发性流产(CBA/J×DBA/2)小鼠模型,即NP模型和RSA模型,实验组分别给予kisspeptin10和kisspeptin234(阻滞剂)隔日1次腹腔注射(RSA-KP10组和NP-KP234组),对照组给予生理盐水隔日1次腹腔注射(RSA-NS组和NP-NS组),共4组,每组6只,取孕9.5 d的子宫组织,观察胚胎吸收的情况,检测上述蛋白和基因的表达。结果与正常妊娠(NP)女性相比,RSA患者kisspeptin、IGFBP1、Notch1、Akt、Foxo1的蛋白及其基因表均降低(P<0.01或P<0.05)。hESC添加kisspeptin10后蜕膜化能力增强,Notch1、Akt、Foxo1的蛋白及基因表达增加,干扰KISS1后表达降低;免疫荧光见增殖状态的hESC呈细长形,蜕膜化后细胞变圆、变大,Notch1表达增加,添加kisspeptin10后蜕膜化程度增高,干扰KISS1后减弱;分别用Notch1、Akt、Foxo1抑制剂处理hESC,发现三者按Notch1/Akt/Foxo1的顺序依次调控(P<0.05)。与注射生理盐水组相比,正常妊娠小鼠注射kisspeptin234后吸收胎增多(P<0.001),IGFBP1、Notch1、Akt、Foxo1的蛋白及基因表达下降(P<0.05);复发流产小鼠注射kisspeptin10后吸收胎减少(P<0.001),上述蛋白及基因表达升高(P<0.05)。结论 kisspeptin可能通过Notch1/Akt/Foxo1信号通路调控子宫内膜蜕膜化,其表达下调导致蜕膜化不良,可能是导致RSA发病的原因之一。

关键词： kisspeptin Notch1 子宫内膜蜕膜化复发性流产

Excellent shape recovery characteristics of Cu-Al-Mn-Fe shape memory single crystal

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Journal of Materials Science & Technology 2020年第22期54卷 43-50页

作者： Shuiyuan Yang Lipeng guo Xinyu Qing Shen Hong Jixun Zhang Mingpei Li cuiping Wang Xingjun Liu College of Materials and Fujian Key Laboratory of Materials Genome Xiamen UniversityXiamen361005China State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology UniversityShenzhen518055China

Shape memory alloys can recover the deformed shape due to their superelasticity or shape memory effect. In this study, a novel Cu-Al-Mn-Fe shape memory single crystal is reported. The results show that it has excellent superelasticity and shape memory effect simultaneously when deformed at room temperature, as well as tunably wide response temperature range with near-zero interval of reverse phase transformation. When deforming one single crystal at room temperature, it not only possesses full superelasticity of 7%, but also tunable shape memory effects up to 8.8 %. The full shape recovery during heating exhibits near-zero response interval and tunably wide response temperature range of 166 K depending on the deformation. The functional characteristics of the alloys result from the controllable reverse phase transformation hinging on the stabilization of stress-induced martensite. This class of Cu-Al-Mn-Fe alloy may be used as both superelastic materials, and shape memory materials with wide working temperature range as high-sensitive detector, driver or sensor.

关键词： Shape memory alloys Superelasticity Martensite stabilization Shape memory effect Nanoparticles

Phosphorus-doped lithium- and manganese-rich layered oxide cathode material for fast charging lithium-ion batteries

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Journal of Energy Chemistry 2021年第11期30卷 538-545,I0012页

作者： Yuqiong Kang Xingang guo Zhiwu guo Jiangang Li Yunan Zhou Zheng Liang cuiping Han Xiangming He Yun Zhao Naser Tavajohi Baohua Li School of Chemical Engineering Beijing Institute of Petrochemical TechnologyBeijing 102617China Engineering Laboratory for Next Generation Power and Energy Storage Batteries Graduate School at ShenzhenTsinghua UniversityShenzhen 518055GuangdongChina School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesShanghai Jiao Tong UniversityShanghai 200240China Institute of Nuclear&New Energy Technology Tsinghua UniversityBeijing 100084China Department of Chemistry Chemical-Biological CentreUmeåUniversityUmeå90187Sweden Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology Beijing 102617China

Owing to their high theoretical specific capacity and low cost, lithium- and manganese-rich layered oxide (LMR) cathode materials are receiving increasing attention for application in lithium-ion batteries. However, poor lithium ion and electron transport kinetics plus side effects of anion and cation redox reactions hamper power performance and stability of the LMRs. In this study, LMR Li_(1.2)Mn_(0.6)Ni_(0.2)O_(2) was modified by phosphorus (P)-doping to increase Li+ conductivity in the bulk material. This was achieved by increasing the interlayer spacing of the lithium layer, electron transport and structural stability, resulting in improvement of the rate and safety performance. P^(5+) doping increased the distance between the (003) crystal planes from ~0.474 nm to 0.488 nm and enhanced the structural stability by forming strong covalent bonds with oxygen atoms, resulting in an improved rate performance (capacity retention from 38% to 50% at 0.05 C to 5 C) and thermal stability (50% heat release compared with pristine material). First-principles calculations showed the P-doping makes the transfer of excited electrons from the valence band to conduction band easier and P can form a strong covalent bond helping to stabilize material structure. Furthermore, the solid-state electrolyte modified P5+ doped LMR showed an improved cycle performance for up to 200 cycles with capacity retention of 90.5% and enhanced initial coulombic efficiency from 68.5% (pristine) or 81.7% (P-doped LMR) to 88.7%.

关键词： Lithium-ion battery Lithium-and manganese-rich layered oxide Phosphorus doping High-rate performance

African Swine Fever Virus MGF360-12L Inhibits Type Ⅰ Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway

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Virologica Sinica 2021年第2期36卷 176-186页

作者： Yisha Zhuo Zeheng guo Tongtong Ba Cheng Zhang Lihua He cuiping Zeng Hanchuan Dai College of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070China

African swine fever(ASF)is an infectious transboundary disease of domestic pigs and wild boar and spreading throughout *** is no vaccine and treatment *** immune escape strategies of African swine fever virus(ASFV)are crucial factors affecting immune prevention and vaccine ***360 genes have been implicated in the modulation of the IFN-Ⅰ*** molecular mechanisms contributing to innate immunity are poorly *** this study,we demonstrated that ASFV MGF360-12 L(MGF360 families 12 L protein)significantly inhibited the mRNA transcription and promoter activity of IFN-βand NF-κB,accompanied by decreases of IRF3,STING,TBK1,ISG54,ISG56 and AP-1 m RNA ***,MGF360-12 L might suppress the nuclear localization of p50 and p65 mediated by classical nuclear localization signal(NLS).Additionally,MGF360-12 L could interact with KPNA2,KPNA3,and KPNA4,which interrupted the interaction between p65 and KPNA2,KPNA3,*** further found that MGF360-12 L could interfere with the NF-κB nuclear translocation by competitively inhibiting the interaction between NF-κB and nuclear transport *** findings suggested that MGF360-12 L could inhibit the IFN-Ⅰproduction by blocking the interaction of importinαand NF-κB signaling pathway,which might reveal a novel strategy for ASFV to escape the host innate immune response.

关键词： African swine fever virus(ASFV) MGF360-12L IFN-Ⅰ NF-κB Nuclear transport

A BLIND AUDIO STEGANALYSIS BASED ON FEATURE FUSION

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Journal of Electronics(China) 2011年第3期28卷 265-276页

作者： Wei Yifang guo Li Wang Yujie Wang cuiping Department of Electronic Science and Technology University of Science and Technology of China Hefei 230027 China

In this paper, we present a blind steganalysis based on feature fusion. Features based on Short Time Fourier Transform (STFT), which consists of second-order derivative spectrum features of audio and Mel-frequency cepstrum coefficients, audio quality metrics and features on linear prediction residue are extracted separately. Then feature fusion is conducted. The performance of the proposed steganalysis is evaluated against 4 steganographic schemes: Direct Sequence Spread Spectrum (DSSS), Quantization Index Modulation (QIM), ECHO embedding (ECHO), and Least Significant Bit em-bedding (LSB). Experiment results show that the classifying performance of the proposed detector is much superior to the previous work. Even more exciting is that the proposed methodology could detect the four steganography, with 85%+ classification accuracy achieved in all the detections, which makes the proposed steganalysis methodology capable of being regarded as a blind steganalysis, and especially useful when the steganalyzer are without the knowledge of the steganographic scheme employed in data embedding.

关键词： Feature fusion Steganalysis Mel-cepstrum Second-order derivative Audio quality metrics Linear prediction

Two-phase flow in anode flow field of a small direct methanol fuel cell in different gravities

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Science China(Technological Sciences) 2009年第6期52卷 1576-1582页

作者： guo Hang WU Feng YE Fang ZHAO JianFu WAN ShiXin Lücuiping MA ChongFang Key Laboratory of Enhanced Heat Transfer and Energy Conservation Ministry of Education of ChinaCollege of Environmental and Energy EngineeringBeijing University of TechnologyBeijing 100124China Key Laboratory of Heat Transfer and Energy Conversion Beijing MunicipalityBeijing University of TechnologyBeijing 100124China Institute of Mechanics Chinese Academy of SciencesBeijing 100080China

An in-situ visualization of two-phase flow inside anode flow bed of a small liquid fed direct methanol fuel cells in normal and reduced gravity has been conducted in a drop *** anode flow bed con-sists of 11 parallel straight *** length,width and depth of single channel,which had rec-tangular cross section,are 48.0,2.5 and 2.0mm,*** rib width was *** experi-mental results indicated that when the fuel cell orientation is vertical,two-phase flow pattern in anode channels can evolve from bubbly flow in normal gravity into slug flow in *** size of bub-bles in the reduced gravity is also *** microgravity,the bubbles rising speed in vertical channels is obviously slower than that in normal *** the fuel cell orientation is horizontal,the slug flow in the reduced gravity has almost the same characteristic with that in normal *** implies that the effect of gravity on two-phase flow is small and the bubbles removal is governed by viscous *** the gas slugs or gas columns occupy channels,the performance of liquid fed direct methanol fuel cells is failing *** infers that in long-term microgravity,flow bed and operating condition should be optimized to avoid concentration polarization of fuel cells.

关键词： direct methanol fuel cells microgravity two-phase flow visualization bubble behavior

MR-STIR SPACE在婴幼儿臂丛神经损伤中的诊断价值

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医学影像学杂志 2021年第7期31卷 1111-1114页

作者：韩晶劳群郭翠萍王彪陈琬杨昕丁忠祥杭州市儿童医院放射科浙江杭州310014 郑州儿童医院放射科河南郑州450007 杭州市第一人民医院放射科浙江杭州310006

目的探讨重T2磁共振成像压脂技术(MR-STIR SPACE)在婴幼儿臂丛神经损伤中的诊断价值。方法选取临床诊断臂丛神经损伤的28例患儿临床肌力、肌电图和MRI资料,用Kappa检验判断临床肌力、MRI两者及肌电图、MRI两者间的一致性,P<0.05为差异... 详细信息

目的探讨重T2磁共振成像压脂技术(MR-STIR SPACE)在婴幼儿臂丛神经损伤中的诊断价值。方法选取临床诊断臂丛神经损伤的28例患儿临床肌力、肌电图和MRI资料,用Kappa检验判断临床肌力、MRI两者及肌电图、MRI两者间的一致性,P<0.05为差异有统计学意义。结果28例患儿中,单侧臂丛神经损伤25例,双侧均有损伤3例,共计损伤31侧,其中左臂丛神经11侧,右臂丛神经20侧。主要为神经根的损伤,表现为神经根消失、离断、增粗、扩张。31侧损伤的臂丛神经分别与临床肌力一一对照,一致性较好(κ=0.712,P<0.05);与肌电图一一对照,一致性仍较好(κ=0.633,P<0.05)。结论MR-STIR SPACE在婴幼儿臂丛神经损伤的诊断中具有较高的价值,可作为婴幼儿臂丛神经损伤筛查的重要依据。

关键词：臂丛神经损伤磁共振成像婴幼儿

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基于逻辑回归的个人信用评分卡模型研究

现代信息科技 2024年第5期8卷 12-16页

作者：张俊丽郭双颜任翠萍马倩西安欧亚学院陕西西安710065 交通银行陕西省分行陕西西安710004

构建有效的个人信用风险评价系统,用以应对潜在的个人信贷风险,这对金融行业和社会公众皆有重要的现实意义。文章首先对数据进行清洗、预处理,然后通过WOE编码分箱、IV值进行变量筛选,构建了逻辑回归模型并基于逻辑回归模型建立了个人... 详细信息

构建有效的个人信用风险评价系统,用以应对潜在的个人信贷风险,这对金融行业和社会公众皆有重要的现实意义。文章首先对数据进行清洗、预处理,然后通过WOE编码分箱、IV值进行变量筛选,构建了逻辑回归模型并基于逻辑回归模型建立了个人信用评分卡模型,该模型可辅助决策者制定合理的授信政策、定价策略以及其他相关业务运营策略。

关键词：个人信用评估评分卡 AUC

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草乌的研究进展及质量标志物预测

中华中医药学刊 2024年第1期42卷 139-147页

作者：高雅郭文勇廖翠平阿拉坦朝鲁门额尼日勒图雅中国中医科学院医学实验中心北京100700 中国中医科学院中医临床基础医学研究所北京100700 内蒙古民族大学蒙医药学院内蒙古通辽028000 中国中医科学院中医药研究发展中心北京100700

草乌药用价值极高,在中医、蒙医等领域具有悠久的用药历史,但因其中毒剂量和治疗剂量较为接近,治疗窗狭窄,其临床应用受到一定限制,因此全方位建立草乌的质量标准评价体系是十分必要的。从基源考证、化学成分和药理作用等几个方面对草... 详细信息

草乌药用价值极高,在中医、蒙医等领域具有悠久的用药历史,但因其中毒剂量和治疗剂量较为接近,治疗窗狭窄,其临床应用受到一定限制,因此全方位建立草乌的质量标准评价体系是十分必要的。从基源考证、化学成分和药理作用等几个方面对草乌的研究现状进行综述。基于中药质量标志物(Q-marker)理论,针对草乌活性毒性的药物特性,结合其化学成分、现代药理毒理活性研究等方面,推测乌头碱型二萜生物碱类和挥发油可作为其Q-marker,为草乌的质量评价研究提供科学依据。

关键词：草乌化学成分药理作用质量标志物