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A comparative study of the anti-fatigue activity of extracts from different parts of Cistanche tubulosa(Schenk)Wight Author links open overlay panel

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Journal of Traditional Chinese Medical Sciences 2024年第2期11卷 222-231页

作者： Jianteng Dong Junjun Li Yizhou Liu Lingwen Cui Xiangning Liu Gang Wang Qixin Wang David N.criddle Pengfei Tu Chun Li School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China Modern Research Center for Traditional Chinese Medicine School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing102488China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing100700China Department of Molecular&Clinical Cancer Medicine Institute of SystemsMolecular&Integrative BiologyUniversity of LiverpoolLiverpoolL693BXUK Peking University School of Pharmaceutical Sciences Beijing100191China

Objective To evaluate the anti-fatigue effects of different extracts from Cistanche tubulosa(Schenk)Wight(***,Rou Cong Rong),focusing on central and exercise-induced fatigue in *** study investigated the pharmacological effects of the total oligosaccharides,polysaccharides,and phenylethanoid glycosides(CPhGs)extracted from *** Models of sleep deprivation and forced swimming fatigue were established to simulate central and exercise-induced *** mice were treated with different extracts of ***,and their effects were assessed using behavioral tests to measure exercise capacity,learning,and memory *** analyses were performed to evaluate the changes in serum and brain neurotransmitter levels,liver and muscle glycogen storage,and various fatigue-related *** This study found that treatment with *** extract improved exercise capacity,learning,and memory in *** oligosaccharides from *** enhanced adrenocorticotropic hormone,cholinesterase,and thyroid-stimulating hormone levels,reduced cortisol levels in central fatigue models,and ameliorated biochemical markers of exercise-induced fatigue,including lowering lactic acid,blood urea nitrogen,and malondialdehyde *** the tested extracts,the total oligosaccharides showed the most comprehensive anti-fatigue *** The anti-fatigue effects of ***,particularly those of its total oligosaccharides,are pronounced in both central and exercise-induced *** effects are mediated by the regulation of neurotransmitter levels,enhancement of glycogen storage,and improvement of antioxidant enzyme activity,suggesting potential therapeutic benefits in fatigue-related conditions.

关键词： Cistanche tubulosa Anti-fatigue Central fatigue Exercise fatigue Oligosaccharides

Radical innovation breakthroughs of biodegradation of plastics by insects:history,present and future perspectives

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Frontiers of Environmental Science & Engineering 2024年第6期18卷 127-165页

作者： Shan-Shan Yang Wei-Min Wu Federica Bertocchini Mark Eric Benbow Suja P.Devipriya Hyung Joon Cha Bo-Yu Peng Meng-Qi Ding Lei He Mei-Xi Li Chen-Hao Cui Shao-Nan Shi Han-Jun Sun Ji-Wei Pang Defu He Yalei Zhang Jun Yang Deyi Hou De-Feng Xing Nan-Qi Ren Jie Ding Craig S.criddle State Key Laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin 150090China Department of Civil and Environmental Engineering William&Cloy Codiga Resource Recovery CenterStanford UniversityStanfordCA 94305USA Plasticentropy rue Thiers 28Reims 51100France Department of Entomology and Department of Osteopathic Medical Specialties Michigan State UniversityEast LansingMI 48824USA School of Environmental Studies Cochin University of Science and TechnologyKochi 682022India Department of Chemical Engineering Pohang University of Science and TechnologyPohang 37673Republic of Korea School of Environmental Science and Engineering Tongji UniversityShanghai 200092China China Energy Conservation and Environmental Protection Group CECEP Talroad Technology Co.Ltd.Beijing 100096China School of Ecological and Environmental Sciences East China Normal UniversityShanghai 200241China Research Institute for Frontier Science Beihang UniversityBeijing 100191China School of Environment Tsinghua UniversityBeijing 100084China

Insects damaging and penetrating plastic packaged materials has been reported since the *** innovation breakthroughs of plastic biodegradation have been initiated since the discovery of biodegradation of plastics by Tenebrio molitor larvae in 2015 followed by Galleria mellonella in *** we review updated studies on the insect-mediated biodegradation of *** biodegradation by insect larvae,mainly by some species of darkling beetles(Tenebrionidae)and pyralid moths(Pyralidae)is currently a highly active and potentially transformative area of *** the past eight years,publications have increased explosively,including discoveries of the ability of different insect species to biodegrade plastics,biodegradation performance,and the contribution of host and microbiomes,impacts of polymer types and their physic-chemical properties,and responsible enzymes secreted by the host and gut *** date,almost all major plastics including polyethylene(PE),polypropylene(PP),polyvinyl chloride(PVC),polyethylene terephthalate(PET),polyurethane(PUR),and polystyrene(PS)can be biodegraded by *** and ten other insect species representing the Tenebrionidae and Pyralidae *** biodegradation processes are symbiotic reactions or performed by synergistic efforts of both host and gut-microbes to rapidly depolymerize and biodegrade plastics with hourly *** digestive ezymens and bioreagents screted by the insects play an essential role in plasatic biodegradation in certain species of Tenebrionidae and Pyralidae *** research on the insect itself,gut microbiomes,transcriptomes,proteomes and metabolomes has evaluated the mechanisms of plastic biodegradation in *** conclude this review by discussing future research perspectives on insect-mediated biodegradation of plastics.

关键词： Plastics Biodegradation Insects Gut microbiomes Enzymes

Microplastics pollution and reduction strategies

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Frontiers of Environmental Science & Engineering 2017年第1期11卷 1-4页

作者： Wei-Min Wu Jun Yang Craig S. criddle Department of Civil and Environmental Engineering William & Cloy Codiga Resource Recovery Center Center for Sustainable Development & Global Competitiveness Stanford University Stanford CA 94305-4020 USA School of Chemistry and Environment Beihang University Beijing 100191 China

Microplastic particles smaller than 5 mm in size are of increasing concem, especially in aquatic environments, such as the ocean. Primary source is microbeads （〈 1 mm） used in cosmetics and cleaning agents and fiber fragments from washing of clothes, and secondary source such as broken down plastic litter and debris. These particles are mostly made from polyethylene （PE）, polypropylene （PP）, polystyrene （PS）, polyethylene terephthalate （PET） and polyesters. They are ingested by diverse marine fauna, including zooplanktons, mussel, oyster, shrimp, fish etc. and can enter human food chains via several pathways. Strategy for control of microplastics pollution should primarily focus on source reduction and subsequently on the development of cost-effective clean up and remediation technologies. Recent research results on biodegradation of plastics have revealed a potential for microbial biodegradation and bioremediation of plastic pollutants, such as PE, PS and PET under appropriate conditions.

关键词： microplastics plastic microbeads environmental pollution biodegradation

Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems

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Journal of Environmental Sciences 2010年第4期22卷 627-634页

作者： Xiaohui Wang Xianghua Wen Craig criddle George Wells Jie Zhang Yin Zhao Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China. Department of Civil and Environmental Engineering Jerry Yang & Akiko Yamazaki Environment & Energy Building Stanford University Stanford CA94305-4020 USA Beijing Drainage Group Co. Ltd. Beijing 100084 China

We investigated the communities of ammonia-oxidizing bacteria （AOB） in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction （PCR） followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of the α-subunit of the ammonia monooxygenase gene （amoA）. The T-RFLP fingerprint analyses showed that different wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable difference among the AOB T- RFLP profiles from different parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant （WWTP） contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent affected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little affected by the treatment process in this study. Phylogenetic analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.

关键词： activated sludge ammonia-oxidizing bacteria T-RFLP amoA gene wastewater treatment plant

肉苁蓉不同提取部位改善运动性与中枢性疲劳的药效学比较（英文）

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Journal of Traditional Chinese Medical Sciences 2024年第2期 222-231页

作者：董健藤李俊俊刘一舟崔伶雯刘相凝王港王启新 criddle David N. 屠鹏飞李春北京中医药大学中医学院北京中医药大学中药学院中国中医科学中药研究所利物浦大学分子与临床肿瘤医学系北京大学药学院

目的：评估管花肉苁蓉（Cistanche tubulosa (Schenk) Wight，简称肉苁蓉）不同提取物的抗疲劳效果，重点关注中枢和运动诱导的疲劳。本研究调查了从肉苁蓉中提取的总寡糖、多糖和苯乙醇苷的药理作用。方法：建立睡眠剥夺和强制游泳疲... 详细信息

目的：评估管花肉苁蓉（Cistanche tubulosa (Schenk) Wight，简称肉苁蓉）不同提取物的抗疲劳效果，重点关注中枢和运动诱导的疲劳。本研究调查了从肉苁蓉中提取的总寡糖、多糖和苯乙醇苷的药理作用。方法：建立睡眠剥夺和强制游泳疲劳模型，以模拟中枢和运动诱导的疲劳。用肉苁蓉的不同提取物灌胃治疗小鼠，并通过行为学测试评估作用，包括测试运动能力、学习和记忆能力。生化分析以评估血清和大脑神经递质水平、肝脏和肌糖原储存以及各种与疲劳相关的生物标志物的变化。结果：研究发现，使用肉苁蓉提取物处理可以改善小鼠的运动能力、学习和记忆水平。肉苁蓉的总寡糖提高了促肾上腺皮质激素、胆碱酯酶和促甲状腺激素水平，在中枢疲劳模型中降低了皮质酮水平，并改善了运动诱导疲劳的生化标志物，包括降低乳酸、血尿素氮和丙二醛水平。在测试的不同提取物中，总寡糖显示出最全面的抗疲劳效果。结论：肉苁蓉的抗疲劳效果，尤其是其总寡糖的效果，在中枢和运动诱导的疲劳中均表现突出。这些效果是通过调节神经递质水平、增强糖原储存和改善抗氧化酶活性来介导的，表明其在疲劳相关疾病中的潜在治疗益处。

关键词：肉苁蓉抗疲劳中枢疲劳运动疲劳总寡糖

Phylogenetic diversity of NO reductases,new tools for nor monitoring,and insights into N_(2)O production in natural and engineered environments

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Frontiers of Environmental Science & Engineering 2022年第10期16卷 23-37页

作者： Sung-Geun Woo Holly L.Sewell Craig S.criddle Department of Civil and Environmental Engineering Stanford UniversityStanfordCA 94305USA NSF Engineering Research Center Re-Inventing the Nation's Urban Water Infrastructure(ReNUWIt)Stanford UniversityStanfordCA 94305USA

Nitric oxide reductases(NORs)have a central role in denitrification,detoxification of nitric oxide(NO)in host-pathogen interactions,and NO-mediated cell-cell *** this study,we focus on the phylogeny and detection of qNOR and cNOR genes because of their nucleotide sequence similarity and evolutionary relatedness to cytochrome oxidases,their key role in denitrification,and their abundance in natural,agricultural,and wastewater *** also include nitric oxide dismutase(NOD)due to its similarity to *** 548 nor sequences from publicly accessible databases and sequenced isolates from N2O-producng bioreactors,we constructed phylogenetic trees for 289 qnor/nod genes and 259 cnorB *** trees contain evidence of horizontal gene transfer and gene duplication,with 13.4%of the sequenced strains containing two or more nor *** aligning amino acid sequences for qnor+cnor,qnor,and cnor,we identified four highly conserved regions for NOR and NOD,including two highly conserved histidine residues at the active site for qNOR and *** this approach,we identified conserved sequences for:1)all nor(nor-universal);2)all qnor(qnor-universal)and all cnor(cnor-universal);3)qnor of Comamonadaceae;4)Clade-specific sequences;and 5)nod of Candidates Methylomirabilis *** of primer performance were confirmed experimentally.

关键词： N_(2)O Greenhouse gas NO reductase NO dismutase Primer Crystal structure

Uranium sequestration in sediment at an iron-rich contaminated site at Oak Ridge, Tennessee *** followed by reoxidation

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Journal of Environmental Sciences 2019年第11期31卷 156-167页

作者： Peng-Song Li Wei-Min Wu Debra H.Phillips David B.Watson Shelly Kelly Bing Li Tonia Mehlhorn Kenneth Lowe Jennifer Earles Hu-Chun Tao Tong Zhang Craig S.criddle Department of Civil and Environmental Engineering William & Cloy Codiga Resource Recovery Center Center for Sustainable Development & Global Competitiveness Stanford University Stanford CA 94305-4020 USA Key Laboratory for Heavy Metal Pollution Control and Reutilization School of Environment and Energy Peking University Shenzhen Graduate School Shenzhen 518055 China Institute of New Energy Technology Tsinghua University Beijing 100084 China School of Natural and Built Environment Queen's University Belfast Belfast BT9 5AG Northern Ireland UK Environmental Sciences Division Oak Ridge National Laboratory Oak Ridge P.O.Box 2008 TN 37831 USA EXAFS Analysis Bolingbrook IL 60440 USA Department of Civil Engineering The University of Hong Kong Hong Kong China

This study evaluated uranium sequestration performance in iron-rich (30 g/kg) sediment via bioreduction followed by *** tests (1383 days) at Oak Ridge,Tennessee demonstrated that uranium contents in sediments increased after bioreduced sediments were re-exposed to nitrate and oxygen in contaminated *** of contaminated sediments (1200 mg/kg U) with ethanol in microcosm reduced aqueous U from 0.37 to 0.023 mg/*** of the bioreduced sediment were reoxidized with O2,H2O2,and NaNO3,respectively,over 285 days,resulting in aqueous U of 0.024,1.58 and 14.4 mg/L at pH 6.30,6.63 and 7.62,*** source-and the three reoxidized sediments showed different desorption and adsorption behaviors of U,but all fit a Freundlich *** adsorption capacities increased sharply at pH 4.5 to 5.5,plateaued at pH 5.5 to 7.0,then decreased sharply as pH increased from 7.0 to *** O2-reoxidized sediment retained a lower desorption efficiency at pH over *** NO3--reoxidized sediment exhibited higher adsorption capacity at pH 5.5 to *** pH-dependent adsorption onto Fe(Ⅲ) oxides and formation of U coated particles and precipitates resulted in U sequestration,and bioreduction followed by reoxidation can enhance the U sequestration in sediment.

关键词： Sediments Uranium Sequestration Bioreduction Reoxidation

Microbial communities biostimulated by ethanol during uranium （VI） bioremediation in contaminated sediment as shown by stable isotope probing

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Frontiers of Environmental Science & Engineering 2015年第3期9卷 453-464页

作者： Mary Beth LEIGH Wei-Min WU Erick CARDENAS Ondrej UHLIK Sue CARROLL Terry GENTRY Terence L. MARSH Jizhong ZHOU Philip JARDINE Craig S. criddle James M. TIEDJE Center for Microbial Ecology Michigan State University East Lansing MI 48824 USA Institute of Arctic Biology University of Alaska Fairbanks Fairbanks AK 99775 USA Department of Civil and Environmental Engineering Center for Sustainable Development & Global Competitiveness Codiga Resource Recovery Center Stanford University Stanford CA 94305-4020 USA Department of Biochemistry and Microbiology Institute of Chemical Technology Prague Technicka 3 166 28 Prague Czech Republic Environmental Sciences Division Oak Ridge National Laboratory Oak Ridge TN 37831 USA Department of Botany and Microbiology University of Oklahoma Norman OK 73019 USA Department of Crop and Soil Sciences Texas A&M University College Station TX 77843 USA

Stable isotope probing （SIP） was used to identify microbes stimulated by ethanol addition in microcosms containing two sediments collected from the bioremediation test zone at the US Department of Energy Oak Ridge site, TN, USA. One sample was highly bioreduced with ethanol while another was less reduced. Microcosms with the respective sediments were amended with ^13C labeled ethanol and incubated for 7 days for SIP. Ethanol was rapidly converted to acetate within 24h accompanied with the reduction of nitrate and sulfate. The accumulation of acetate persisted beyond the 7 d period. Aqueous U did not decline in the microcosm with the reduced sediment due to desorption of U but continuously declined in the less reduced sample. Microbial growth and concomitant 13C-DNA production was detected when ethanol was exhausted and abundant acetate had accumulated in both microcosms. This coincided with U（VI） reduction in the less reduced sample. I3C originating from ethanol was ultimately utilized for growth, either directly or indirectly, by the dominant microbial community members within 7 days of incubation. The microbial community was comprised predominantly of known denitrifiers, sulfate-reducing bacteria and iron （Ⅲ） reducing bacteria including Desulfovibrio, Sphingomonas, Ferribacterium, Rhodanobacter, Geothrix, Thiobacillus and others, including the known U（VI）-redueing bacteria Acidovorax, Anaeromyxobacter, Desulfovibrio, Geobac- ter and Desulfosporosinus. The findings suggest that ethanol biostimulates the U（VI）-reducing microbial com- munity by first serving as an electron donor for nitrate, sulfate, iron （IH） and U（VI） reduction, and acetate which then functions as electron donor for U（VI） reduction and carbon source for microbial growth.

关键词： Stable isotope probing (S/P) ethanol acetate uranium reduction sediment bioremediation

城市污水处理厂活性污泥中氨氧化菌群落结构研究

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环境科学 2009年第10期30卷 3002-3006页

作者：王晓慧文湘华 criddle Craig 张杰杨宁宁赵颖清华大学环境科学与工程系北京100084 Department of Civil and Environmental Engineering Stanford University 北京排水集团北京100044

为了解析污水处理厂中氨氧化菌(AOB)的群落结构,以及处理工艺、规模等参数对AOB群落结构的影响,采用针对AOB功能基因氨单加氧酶(amoA)的末端限制性片段长度多态性技术(T-RFLP)、克隆测序等方法,研究了北京市6个污水处理厂的9个污水处理... 详细信息

为了解析污水处理厂中氨氧化菌(AOB)的群落结构,以及处理工艺、规模等参数对AOB群落结构的影响,采用针对AOB功能基因氨单加氧酶(amoA)的末端限制性片段长度多态性技术(T-RFLP)、克隆测序等方法,研究了北京市6个污水处理厂的9个污水处理系统中AOB的群落结构.T-RFLP指纹图谱表明不同污水处理系统中AOB的群落结构有所不同,主要的末端限制性片段(T-RF)为354、491和291 bp.T-RFLP指纹图谱及聚类分析表明,污水处理工艺对系统中AOB的群落结构影响较小,而处理规模对AOB的群落结构有一定的影响.对功能基因amoA的系统发育分析表明,污水处理系统中优势AOB均为Nitrosomonasspp.,而非Nitrosospiraspp..这可能是Nitrosomonasspp.的最大比增长速率(μmax)较高,使其更容易成为活性污泥系统中的优势AOB.

关键词：氨氧化菌(AOB) 末端限制性片段长度多态性技术(T-RFLP) 氨单加氧酶(amoA) 城市污水处理厂