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Degradation of intestinal mRNA:A matter of treatment

World Journal of Gastroenterology 2015年第12期21卷 3499-3508页

作者： Sabine Heumüller-Klug Carsten Sticht Karin Kaiser Elvira Wink cornelia hagl Lucas Wessel Karl-Herbert Schafer Department of Pediatric Surgery Mannheim Medical University of Heidelberg Medical Research Centre Mannheim Medical University of Heidelberg University of Applied Sciences Kaiserslautern

AIM: To characterize the influence of location, species and treatment upon RNA degradation in tissue samples from the gastrointestinal tract. METHODS: The intestinal samples were stored in different medium for different times under varyingconditions: different species(human and rat), varying temperature(storage on crushed ice or room temperature), time point of dissection of the submucous-mucous layer from the smooth muscle(before or after storage), different rinsing methods(rinsing with Medium, PBS, RNALater or without rinsing at all) and different regions of the gut(proximal and distal small intestine, caecum, colon and rectum). The total RNA from different parts of the gut(rat: proximal and distal small intestine, caecum, colon and rectum, human: colon and rectum) and individual gut layers(muscle and submucosal/mucosal) was extracted. The quality of the RNA was assessed by micro capillary electrophoresis. The RNA quality was expressed by the RNA integrity number which is calculated from the relative height and area of the 18 S and 28 S RNA peaks. From rat distal small intestine q PCR was performed for neuronal and glial ***: RNA obtained from smooth muscle tissue is much longer stable than those from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, on ice it is stable at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. RNA obtained from the submucosal/mucosal layer always showed a much worse amplification rate than RNA from muscle tissue. In general RNA harvested from rat tissue, either smooth muscle layer or submucosal/mucosal layer is much longer stable than RNA from human gut tissue, and RNA obtained from smooth muscle tissue shows an increased stability compared to RNA from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, while the stabilit

关键词： Key words:Intestinal RNA Degradation Storing conditions Flushing Cooling

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Ink-structing the future of vascular tissue engineering:a review of the physiological bioink design

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Bio-Design and Manufacturing 2024年第2期7卷 181-205页

作者： Judith Synofzik Sebastian Heene Rebecca Jonczyk cornelia Blume Institute for Technical Chemistry Leibniz University Hannover30167 HannoverGermany

Three-dimensional(3D)printing and bioprinting have come into view for a plannable and standardizable generation of implantable tissue-engineered constructs that can substitute native tissues and *** tissue-engineered structures are intended to integrate with the patient’s *** tissue engineering(TE)is relevant in TE because it supports the sustained oxygenization and nutrition of all tissue-engineered *** have a specific role,representingthenecessarymedium for printability and vascular cell *** review aims to understand the requirements for the design of vascular ***,an in-depth analysis of vascular cell interaction with their native environment must be gained.A physiological bioink suitable for a tissue-engineered vascular graft(TEVG)must not only ensure good printability but also induce cells to behave like in a native vascular vessel,including self-regenerative and growth *** review describes the general structure of vascular walls with wall-speciﬁc cell and extracellular matrix(ECM)components and biomechanical properties and ***,the physiological role of vascular ECM components for their interaction with vascular cells and the mode of interaction is *** currently available or imaginable bioinks are described from physiological matrix proteins to nonphysiologically occurring but natural chemical compounds useful for vascular *** physiological performance of these bioinks is evaluated with regard to biomechanical properties postprinting,with a view to current animal studies of 3D printed vascular ***,the main challenges for further bioink development,suitable bioink components to create a self-assembly bioink concept,and future bioprinting strategies are *** concepts are discussed in terms of their suitability to be part of a TEVG with a high potential for later clinical use.

关键词： Vascular wall histology Vascular cells Microenvironment Extracellular matrix Cell–matrix interaction Bioink Printability

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Deep learning-based detection of indicator species for monitoring biodiversity in semi-natural grasslands

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Environmental Science and Ecotechnology 2024年第5期21卷 238-247页

作者： Deepak H.Basavegowda Inga Schleip Paul Mosebach cornelia Weltzien Technische Universitat Berlin Straße des 17.Juni 13510623BerlinGermany Leibniz-Institut für Agrartechnik und Biookonomie e.V.(ATB) Max-Eyth-Allee 10014469PotsdamGermany Hochschule für nachhaltige Entwicklung Eberswalde Schicklerstraße 516225EberswaldeGermany

Deep learning(DL)has huge potential to provide valuable insights into biodiversity changes in speciesrich agricultural ecosystems such as semi-natural grasslands,helping to prioritize and plan conservation ***,DL has been underexplored in grassland conservation efforts,hindered by data scarcity,intricate ecosystem interactions,and limited economic ***,we developed a DL-based object-detection model to identify indicator species,a group of vascular plant species that serve as surrogates for biodiversity assessment in high nature value(HNV)*** selected indicator species Armeria maritima,Campanula patula,Cirsium oleraceum,and Daucus *** overcome the hurdle of limited data,we grew indicator plants under controlled greenhouse conditions,generating a sufficient dataset for DL model *** model was initially trained on this greenhouse ***,smaller datasets derived from an experimental grassland plot and natural grasslands were added to the training to facilitate the transition from greenhouse to field *** optimized model achieved remarkable average precision(AP)on test datasets,with 98.6 AP50 on greenhouse data,98.2 AP50 on experimental grassland data,and 96.5 AP50 on semi-natural grassland *** findings highlight the innovative application of greenhouse-grown specimens for the in-situ identification of plants,bolstering biodiversity monitoring in grassland ***,the study illuminates the promising role of DL techniques in conservation programs,particularly as a monitoring tool to support result-based agrienvironment schemes.

关键词： Species-rich grasslands Biodiversity monitoring Object detection Cross-domain knowledge transfer

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Eight of ten patients return to daily activities, work, and sports after total knee arthroplasty

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World Journal of Orthopedics 2024年第7期15卷 608-617页

作者： Maud cornelia Wilhelmina Maria Peters Yvette Pronk Justus-Martijn Brinkman Department of Research Kliniek ViaSanaMill 5451 AANetherlands Department of Orthopedic Surgery Kliniek ViaSanaMill 5451 AANetherlands

BACKGROUND Besides return to work(RTW)and return to sports(RTS),patients also prefer to return to daily activities(RTA)such as walking,sleeping,grocery shopping,and domestic work following total knee arthroplasty(TKA).However,evidence on the timelines and probability of patients’RTA is *** To assess the percentage of patients able to RTA,RTW,and RTS after TKA,as well as the timeframe and influencing factors of this *** A retrospective cohort study with prospectively collected data was conducted at a medium-sized Dutch orthopedic *** of RTA,RTW,and RTS were performed at 3 mo and/or 6 mo following *** factors en-compassed patient characteristics,surgical characteristics,and preoperative patient-reported *** TKA patients[n=2063;66 years old(interquartile range[IQR]:7 years);47%male;28 kg/m2(IQR:4 kg/m2)]showed RTA ranging from 28%for kneeling to 94%for grocery shopping,with 20 d(IQR:27 d)spent for putting on shoes to 74 d(IQR:57 d)for *** rates varied from 62%for medium-impact work to 87%for low-impact work,taking 33 d(IQR:29 d)to 78 d(IQR:55 d).RTS ranged from 48%for medium-impact sports to 90%for low-impact sports,occurring within 43 d(IQR:24 d)to 90 d(IQR:60 d).One or more of the investigated factors influenced the return to each of the 14 activities examined,with R²values ranging from 0.013 to *** Approximately 80%of patients can RTA,RTW,and RTS within 6 mo after *** is not consistently in-fluenced by predictive *** help set realistic pre-and postoperative expectations.

关键词： Knee Arthroplasty Replacement Return to work Return to daily activities Return to sports

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Prospective cohort study on facial profile changes in infants with Robin sequence and healthy controls

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World Journal of Pediatrics 2024年第6期20卷 581-589页

作者： cornelia Wiechers Julian Sowula Karen Kreutzer Christoph E.Schwarz Christina Weismann Michael Krimmel Christian F.Poets Bernd Koos Department of Neonatology Tuebingen University HospitalCalwerstrasse 772076 TuebingenGermany Center for Cleft Lip Palate and Craniofacial MalformationsTuebingen University HospitalTuebingenGermany Department of Orthodontics Tuebingen University HospitalTuebingenGermany Department of Oral and Maxillofacial Surgery Tuebingen University HospitalTuebingenGermany

Background Various conservative and surgical approaches exist to treat Robin sequence(RS),but their effects on facial profile and mandibular catch-up growth are unclear.A functional treatment concept,used in our centre for 25 years,includes an individualized palatal plate with a velo-pharyngeal extension and intensive feeding *** We performed a prospective study to objectively describe facial profiles in infants with RS treated with the above *** with isolated RS were admitted to our tertiary perinatal and national referral centre for craniofacial malformations between May 2018 and Nov *** with RS received 3D-photographs during clinically indicated *** controls were recruited from Dec 2018 to Sep 2019 and received 3D-photographs every 3 *** digitally measured jaw index(JI),defined as alveolar overjet(O)x maxillary arch(U)/mandibular arch(L),and the soft tissue reference points A’-point,Nasion’,B’-point angle(ANB’),describing the relative position of maxilla to mandible,were *** mixed models were used to examine time trajectories in JI and ANB’.Results A total of 2073D images,obtained in 19 infants with RS and 32 controls,were *** and ANB’decreased over time in both groups[for JI−0.18(95%CI−0.25 to−0.10);for ANB’:−0.40°per month[(95%CI−0.48 to−0.32)]]but remained lower in controls[for JI−2.5(95%CI−3.2 to−1.8);for ANB’-1.7°(95%CI−2.4 to−1.0)].Also,the ANB’model showed a significant effect of the interaction term diagnosis x *** Based on longitudinal 3D images,we describe changes in objective parameters of facial profile in infants with and without RS during the first year of *** findings indicate catch-up growth in infants treated for RS.

关键词： Infant Mandibular catch-up growth Pre-epiglottic Baton Plate Robin sequence Three-dimensional photography

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A novel homozygous missense variant in ARSK causes MPS X,a new subtype of mucopolysaccharidosis

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Genes & Diseases 2024年第3期11卷 87-90页

作者： Miao Sun cornelia K.Kaminsky Philip Deppe Mai-Brittllse Frederic M.Vaz Barbara Plecko Torben Lubke Linda M.Randolph Division of Genomic Medicine Department of Pathology and Laboratory MedicineChildren's Hospital Los Angeles/Keck School of Medicine of USCLos AngelesCA 90027USA Department of Radiology Children's Hospital Los Angeles/Keck School of Medicine of USCLos AngelesCA 90027USA Department of Chemistry BiochemistryBielefeld UniversityBielefeld 33615Germany Amsterdam UMC Location University of Amsterdam Department of Clinical Chemistry and PediatricsLaboratory Genetic Metabolic DiseasesEmma Children's HospitalMeibergdreef 9Amsterdam 1100 DEthe Netherlands Amsterdam Gastroenterology Endocrinology Metabolism Inborn Errors of MetabolismAmsterdam 1105 BKthe Netherlands Core Facility Metabolomics Amsterdam UMC Location University of AmsterdamAmsterdam 1100DDthe Netherlands Department of Pediatrics Division of General PediatricsMedical Universityof GrazGraz8036Austria Division of Medical Genetics Department of PediatricsChildren's Hospital Los Angeles/Keck School of Medicine of USCLos AngelesCA 90027USA

Mucopolysaccharidoses(MPS)are a group of rare inborn errors of metabolism caused by defective lysosomal enzymes which prevent cells from degrading and recycling certain carbohydrates and fats,resulting in the storage of glycosaminoglycans in cells throughout the *** leads to multisystem abnormalities involving bone,connective tissues,brain,blood,spinal cord,skin,and other tissues.

关键词： metabolism doses polys

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Early clinical outcomes of two regimens of prophylactic antibiotics in cardiac surgical patients with delayed sternal closure

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World Journal of Critical Care Medicine 2024年第3期13卷 32-41页

作者： Mahmoud Ismail Allam Eissa Rasha Kaddoura Danial Hassan cornelia S Carr Samy Hanoura Yasser Shouman Abdulwahid Almulla Amr Salah Omar Department of Cardiothoracic Surgery Hamad Medical CooperationDoha 3050Qatar Department of Anesthesia and Intensive Care Al-Azhar UniversityCairo 11651Egypt Department of Pharmacy Heart HospitalHamad Medical CorporationDoha 3050Qatar Department of Healthcare Profession Harvard TH Chan School of Public HealthMinistry of Public HealthBostonMA 3050United States Department of Surgery Qatar UniversityDoha 3050Qatar Department of Cardiac Anesthesia Hamad Medical CorporationDoha 3050Qatar Department of Anesthesia Weill Cornell Medical College in QatarDoha 3050Qatar Department of Critical Care Medicine Beni Suef UniversityBeni Suef 62521Egypt Department of Medicine Weill Cornell Medical College in QatarDoha 3050Qatar

BACKGROUND Delayed sternal closure(DSC)can be a lifesaving approach for certain patients who have undergone cardiac *** value of the type of prophylactic antibiotics in DSC is still *** To investigate clinical outcomes of different prophylactic antibiotic regimens in patients who had DSC after cardiac *** This was a retrospective observational single-center ***-three consecutive patients who underwent cardiac surgery and had an indication for DSC were *** were subjected to two regimens of antibiotics:Narrow-spectrum and broad-spectrum *** The main outcome measures were length of hospital and intensive care unit(ICU)stay,duration of mechanical ventilation,and *** the 53 patients,12(22.6%)received narrow-spectrum antibiotics,and 41(77.4%)received broad-spectrum *** mean age was 59.0±12.1 years,without significant differences between the *** mean duration of antibiotic use was significantly longer in the broad-spectrum than the narrowspectrum group(11.9±8.7 vs 3.4±2.0 d,P<0.001).The median duration of open chest was 3.0(2.0-5.0)d for all patients,with no difference between groups(P=0.146).The median duration of mechanical ventilation was significantly longer in the broad-spectrum group[60.0(Δinterquartile range(IQR)170.0)h vs 50.0(ΔIQR 113.0)h,P=0.047].Similarly,the median length of stay for both ICU and hospital were significantly longer in the broadspectrum group[7.5(ΔIQR 10.0)d vs 5.0(ΔIQR 5.0)d,P=0.008]and[27.0(ΔIQR 30.0)d vs 19.0(ΔIQR 21.0)d,P=0.031].Five(9.8%)patients were readmitted to the ICU and 18(34.6%)patients died without a difference between *** Prophylactic broad-spectrum antibiotics did not improve clinical outcomes in patients with DSC post-cardiac surgery but was associated with longer ventilation duration,length of ICU and hospital stays vs narrow-spectrum antibiotics.

关键词： Cardiac surgery Delayed sternal closure Intensive care Open chest Prophylactic antibiotics

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MnFe2O4 nanoparticles as new catalyst for oxidative degradation of phenol by peroxydisulfate

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Journal of Environmental Sciences 2017年第3期29卷 269-277页

作者： Marcela Stoia cornelia Muntean Bogdan Militaru Faculty of Industrial Chemistry and Environmental Engineering Politehnica University TimisoaraR0-300223 TimisoaraRomania Research Institute for Renewable Energy Politehnica University TimisoaraRO-300006TimisoaraRomania

Manganese ferrite nanopowder was prepared by thermal decomposition at 400℃ of the gel synthesized from manganese and iron nitrates and polyvinyl alcohol. X-ray diffractometry evidenced that manganese ferrite was formed as single crystalline phase at this temperature. Scanning electron microscope images evidenced the formation of very fine spherical particles （d 〈 11 nm） of manganese ferrite, with specific surface area of 147 m2/g. The powder obtained at 400℃ was used as a catalyst for the oxidative degradation of phenol in aqueous solutions, in the presence of potassium peroxydisulfate as oxidant. High phenol removal efficiencies above 90% were reached at： pH 3-3.5, phenol initial concentration around 50 mg/L, peroxydisulfate：phenol mass ratio 10：1, and catalyst dose 3 g/L. Total organic carbon measurements showed that the degradation of phenol goes, under these conditions, to mineralization in an extent of 60%.

关键词： Manganese ferriteNanoparticlesPhenolPeroxydisulfateCatalytic oxidation

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Thermal Reversion of Plant Phytochromes

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Molecular Plant 2020年第3期13卷 386-397页

作者： cornelia Klose Ferenc Nagy Eberhard Schafer Institute of Biology II University of Freiburg79104 FreiburgGermany Institute of Plant Biology Biological Research CentreTemesvari krt.626726 SzegedHungary

Phytochromes are red/far-red reversible photoreceptors essential for plant growth and *** signaling is mediated by the physiologically active far-red-absorbing Pfr form that can be inactivated to the red-absorbing Pr ground state by light-dependent photoconversion or by light-independent thermal reversion,also termed dark *** the term“dark reversion”is justified by historical reasons and frequently used in the literature,“thermal reversion”more appropriately describes the process of light-independent but temperature-regulated Pfr relaxation that not only occurs in darkness but also in light and is used throughout the *** reversion is a critical parameter for the light sensitivity of phytochrome-mediated responses and has been studied for decades,often resulting in contradictory *** reversion is an intrinsic property of the phytochrome molecules but can be modulated by intra-and intermolecular interactions,as well as biochemical modifications,such as *** this review,we outline the research history of phytochrome thermal reversion,highlighting important predictions that have been made before knowing the molecular *** further summarize and discuss recent findings about the molecular mechanisms regulating phytochrome thermal reversion and its functional roles in light and temperature sensing in plants.

关键词： phytochrome phyA phyB thermal reversion dark reversion in vivo spectroscopy

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Dental stem cells:The role of biomaterials and scaffolds in developing novel therapeutic strategies

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World Journal of Stem Cells 2020年第9期12卷 897-921页

作者： cornelia Larissa Granz Ali Gorji Epilepsy Research Center Westfälische Wilhelms-Universität MünsterMünster 48149Germany Department of Neurosurgery and Department of Neurology Westfälische Wilhelms-UniversitätMünster 48149Germany Shefa Neuroscience Research Center Khatam Alanbia HospitalTehran ***Iran Neuroscience Research Center Mashhad University of Medical SciencesMashhad ***Iran

Dental stem cells(DSCs)are self-renewable cells that can be obtained easily from dental tissues,and are a desirable source of autologous stem *** use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation,high plasticity,immunomodulatory properties,and multipotential *** appropriate scaffolds loaded with favorable biomolecules,such as growth factors,and cytokines,can improve the proliferation,differentiation,migration,and functional capacity of DSCs and can optimize the cellular morphology to build tissue constructs for specific *** enormous variety of scaffolds have been used for tissue engineering with *** these,the scaffolds that particularly mimic tissue-specific micromilieu and loaded with biomolecules favorably regulate angiogenesis,cell-matrix interactions,degradation of extracellular matrix,organized matrix formation,and the mineralization abilities of DSCs in both in vitro and in vivo *** represent a promising cell source for tissue engineering,especially for tooth,bone,and neural tissue *** purpose of the present review is to summarize the current developments in the major scaffolding approaches as crucial guidelines for tissue engineering using DSCs and compare their effects in tissue and organ regeneration.

关键词： Cell transplantation Regenerative medicine Tissue engineering Neural crest Angiogenesis Biomolecules

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