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荧光法测定SD大白鼠不同脑区的单胺类神经递质

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分析化学 1999年第1期27卷 101-103页

作者：汪丛莹庞志功汪宝琪张鹏中国科学院上海脑研究所西安医科大学药学院西安710061 西安医科大学药学院上海200031

以荧光光谱法定量地研究了不同浓度的司考林作用下,鼠脑组织中去甲肾上腺素、多巴胺和5-羟色胺的定量变化,从而表达了中枢神经递质的变化规律.

关键词：荧光法脑组织神经递质

Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

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Agricultural Biotechnology 2020年第5期9卷 9-12,17页

作者： Jing SU Wenjuan wang Shen CHEN congying wang Jianyuan YANG Aiqing Feng Xiaoyuan ZHU The Plant Protection Research Institute Guangdong Academy of Agricultural SciencesGuangzhou 510640China Guangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou 510640China

Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t,designated as Pi2 SNP,Pi9 SNP and Pizt-PA,respectively,have been successfully developed through sequence comparison *** three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus,which facilitates the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R ***,101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers,and the results showed that only 2 out of the 101 cultivars carried Piz-t,but neither Pi2 nor Pi9 band had been *** indicates that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/*** results provide important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.

关键词： Rice Blast Resistance gene Molecular marker MAS Pi2/9 locus

Differential Detection and Analysis of Pathotypes and Differentiation against Xanthomonas oryzae pv. oryzae in Southern China

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Agricultural Biotechnology 2018年第1期7卷 92-96页

作者： Shen CHEN congying wang Jing SU Aiqing FENG Xiaoyuan ZHU Liexian ZENG Guangdong Provincial Key Laboratory of High Technology for Plant Protection Plant Protection Research InstituteGuangdong Academy of Agricultural Sciences

Pathogenic isolates were collected from different rice-cropping regions in southern China to dissect the pathogenic disintegration and variation of Xanthornonas oryzae pv. oryzae （Xoo）. Two sets of rice-Xoo differential hosts, including Chinese system with such five euhivars as IR26, Java14, Nangeng15, Tetep and Jingang 30 and international system with a series of nearisogenic lines （NILs） including IRBB5, IRBB13, IRBB3, IRBB14, IRBB2 and IR24 carrying different known resistance genes,were used to detect pathogenic disintegration for the reaction between host and pathogen with leaf-clippingmethod at the rice booting stage. The results showed the type of the pathogen were divided intosix pathotypes, i.e. , Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ and Ⅸ based on the Chinese differential system, and seven pathogenicraces including RI, R2, R3, R4, R5, R8 and R10 based on the international differential system. The pathogenicity frequency of Xoo pathotypes V and IV and pathogenic races R8 and R5 were 27.40% , 19.30% and 44.67% , 15.34%, which were considered to be the prevailing races in southern China. Pathogenic rates of pathotypes Ⅳ, Ⅴ, Ⅸ and races R8, R5 against 500 varieties derived from southernChina were 96.40% , 95.00% , 50.40% , 62.00% , and 42.60%, respectively. Among which pathotype Ⅸ was the most virulent pathotype. The pathotype Ⅴ became preponderant pathotype and the new pathotype Ⅸ grew up quickly.

关键词： Xanthomonas oryzae pv. oryzae Differential variety Pathotype Race

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无钟炉顶布料器在包钢的使用与发展

钢铁 1998年第4期33卷 5-8页

作者：汪建新郭九春王聪颖包头钢铁学院包头钢铁公司

介绍了由包头钢铁设计研究院自行设计、改进的无钟炉顶布料器在包钢的使用与发展过程。较详细地阐述了ＢＧ－Ⅱ型布料器的结构、工作原理和使用效果。

关键词：布料器无钟炉顶高炉

Resistance Analysis on Major Resistant Genes of Rice Bacterial Blight in Different Donors and Genetic Background

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Agricultural Biotechnology 2017年第5期6卷 11-14,65页

作者： Liexian ZENG congying wang Aqing FENG Chen SHEN Jing SU Wenjiun wang Shengyuan WU Jianyuan YANG Xiaoyuan ZHU The Plant Protection Research Institute Guangdong Academy of Agricultural SciencesGuangzhou 510640China Guangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou 510640China

The different donors of eight rice bacterial blight( BB) major resistant( MR) genes,Xa3,Xa4,xa5,Xa7,Xa10,Xa11,Xa14 and Xa23,were inoculated with two Chinese Xoo strains,strain IV and strain V,respectively. The strain IV was a predominant pathotype in south China,while the strain V was a severe virulentstrain. Two susceptible sterile lines and two susceptible inbred rice were employed to study the effect of geneticbackground on expression of resistance to BB for Xa4,xa5,Xa7 and Xa23. The application value of twodominant resistant genes Xa7 and Xa23 on hybrid rice was also evaluated. The results indicated that the resistance inheritance behaviors of most of the MR genes to the Xoo strains were similar and only a few genes show contradictory reaction in different donors,which suggests that the genetic background would produce an effect on the resistant expression of some R genes. Four resistant donors,IRBB4,IRBB5,IRBB7 and CBB23,were crossed with four susceptible cultivars,WufengA,JitianA,Jingang30 and IR24,respectively,using the resistant donors as male parent. The analysis of F1 populations revealed that the recessive resistance gene xa5 and dominant resistance gene Xa23 conformed to recessive and dominant expression model,respectively,while the other two dominant resistance genes,Xa4 and Xa7,conformed to dominant expression model only in some of F1 populations but not for others. In conclusion,expression of resistance for xa5 and Xa23 was independent of genetic background,while Xa4 and Xa7 show specific behaviors of resistance related to the receptor background. This study also suggests that Xa7 is not suitable for application in hybrid rice,for the F1 generation of its cross with two male sterile lines were highly susceptible,and Xa23( CBB23) had important value in improving BB resistance of hybridrice,as the F1 generations from its cross with 4 susceptible parents,including sterile lines and inbred rice,were all show highly resistant.

关键词： Genetic background Rice bacterial blight Resistance genes Rice Resistance

基于知识传播的产业集群技术升级数值模拟仿真研究

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图书情报工作 2009年第18期53卷 74-78页

作者：胡蓓王聪颖华中科技大学管理学院武汉430074

提出产业集群知识融合的新观点,从进化的角度分析外向型企业推动集群技术演化过程中,知识的感知、选择、吸收以及扩散等阶段的影响作用。基于此构建知识传播各阶段的数值模拟仿真,解释在知识传播过程中传播者数量、企业的关系联系紧密... 详细信息

提出产业集群知识融合的新观点,从进化的角度分析外向型企业推动集群技术演化过程中,知识的感知、选择、吸收以及扩散等阶段的影响作用。基于此构建知识传播各阶段的数值模拟仿真,解释在知识传播过程中传播者数量、企业的关系联系紧密程度以及企业先验知识对传播速度与传播范围的影响大小,从而进一步揭示知识传播的潜在机理和规律。

关键词：外部信息网络内部信息网络外向型企业数值模拟仿真

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改进的蜂群优化聚类集成联合相似度推荐算法

计算机工程 2020年第10期46卷 88-94,102页

作者：王岩王聪英申艳梅河南理工大学计算机科学技术学院河南焦作454000

协同过滤算法由于推荐效果良好,而被广泛应用于推荐领域,但其在数据稀疏及冷启动的情况下会导致推荐效果明显下降。在数据稀疏情况下,为充分利用用户的历史信息以提高算法的推荐精度,提出一种改进的聚类联合相似度推荐算法。采用改进的... 详细信息

协同过滤算法由于推荐效果良好,而被广泛应用于推荐领域,但其在数据稀疏及冷启动的情况下会导致推荐效果明显下降。在数据稀疏情况下,为充分利用用户的历史信息以提高算法的推荐精度,提出一种改进的聚类联合相似度推荐算法。采用改进的蜂群算法来优化K-means++聚类的中心点,使聚类中心在整个数据内达到最优,并对聚类结果进行集成,使得聚类得到进一步优化。根据聚类结果,在同一类中采用改进的用户相似度算法来优化传统相似度算法,使用户间的相似度达到最优,并根据领域的评分预测方法将最佳结果推荐给用户。实验结果表明,该算法的精度、召回率及平均绝对误差均优于其他现有算法,且在数据稀疏情况下其性能仍最佳。

关键词：联合相似度集成聚类 K-means++聚类协同过滤人工蜂群邻域搜索

Comparative Analysis of the Genomes of Three Field Isolates of the Rice Blast Fungus <i>Magnaporthe oryzae</i>from Southern China

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Agricultural Sciences 2021年第7期12卷 713-725页

作者： Kailing Chen Jinqi Feng Shen Chen Jing Su Jianyuan Yang congying wang Aiqing Feng Bing Chen Xiaoyuan Zhu Wenjuan wang Guangdong Provincial Key Laboratory of High Technology for Plant Protection Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou China

Rice blast caused by Magnaporthe oryzae (M. oryzae) is one of the most destructive diseases, which causes significant rice yield losses and affects global food security. To better understand genetic variations among different isolates of M. oryzae in the nature field, we re-sequenced and analyzed the genomes of three field isolates, QJ08-2006, QJ10-10, and QJ10-3001, which showed distinct pathogenicity on Xin-Yin-Zhan, an elite variety in South China. Genome annotation indicated that these three isolates assemblies have similar genome sizes with 38.4 Mb, 38.3 Mb, and 38.4 Mb, respectively. The QJ08-2006 assembly has 2082 contigs with an N50 of 127.4 kb, the QJ10-10 assembly has 2239 contigs with an N50 of 105.13 kb, the QJ10-3001 assembly has 2025 contigs with an N50 of 133.16 kb. A total of 10,432 genes including 1408 putative secreted protein genes were identified from the annotated isolate QJ08-2006 genome, 10,418 genes including 1410 putative secreted protein genes were identified in QJ10-10, and 10,401 genes including 1420 putative secreted protein genes were identified in QJ10-3001. There are as many as 11,076 identical genes in these three isolates and contained only a few unique genes among three isolates, of which 277 unique genes in QJ08-2006 and 264 unique genes in QJ10-10, and 213 unique genes in QJ10-3001. Most of the predicted secreted protein genes had been identified, and the three re-sequenced strains contained 371, 369, and 387 small Indel, respectively. Avr genes were analyzed in several sequenced Magnaporthe strains, the results revealed that Avr-Pi9 and Avr-Piz-t were present in all the sequenced isolates. The isolates QJ08-2006 contained AvrPib, QJ10-10, and QJ10-3001 had an insertion of a Pot3 element in the promoter of the AvrPib gene. Our results showed that, the rapid dominancy of virulence mutant isolates via clonal propagation displayed in the field after the

关键词： Rice Blast Magnaporthe oryzae Comparative Genomics Genetic Variation Secreted Protein Genes

基于信息融合的发展中国家高技术产业集群知识融合与创新模型研究

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图书情报工作 2009年第2期53卷 38-41,73页

作者：胡蓓王聪颖华中科技大学管理学院武汉430074

提出产业集群知识融合的新观点:应用理论研究与实证研究相结合的方法,分析企业对集群外部知识源的学习过程,采用信息融合的DSmT技术,对集群内、外部知识源的知识进行融合,构建基于信息融合的高技术产业集群知识融合与创新模型,通过结构... 详细信息

提出产业集群知识融合的新观点:应用理论研究与实证研究相结合的方法,分析企业对集群外部知识源的学习过程,采用信息融合的DSmT技术,对集群内、外部知识源的知识进行融合,构建基于信息融合的高技术产业集群知识融合与创新模型,通过结构方程模型证实信息融合技术能够提高知识的有效性与全面性,融合后的知识对企业创新绩效有更大的影响作用。

关键词：内向知识联系外向知识联系信息融合DSmT 创新绩效