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Molecular Plant 2016年第7期9卷 1040-1050页

作者： Yan Li con qconcl xia Jinlin Feng Dong Yang Fangming Wu Ying Cao Legong Li Ligeng Ma College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050021 China Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal GovernmentBeijing 100048 China College of Life Sciences Capital Normal University Beijing 100048 China

SKIP is a conserved protein from yeasts to plants and humans. In plant cells, SKIP is a bifunctional regulator that works in the nucleus as a splicing factor by integrating into the spliceosome and as a transcriptional activator by interacting with the Pall complex. In this study, we identified two nuclear localization signals in SKIP and confirmed that each is sufficient to target SKIP to the nucleus. The SNW domain of SKIP is required for both its function as a splicing factor by promoting integration into the spliceosome in response to stress, and its function as a transcriptional activator by controlling its interaction with the Pall complex to participate in flowering. Truncated proteins that included the SNW domain and the N- or C-terminus of SKIP were still able to carry out the functions of the full-length protein in gene splicing and transcriptional activation in Arabidopsis. In addition, we found that SKIP undergoes 26S proteasome-mediated degrada- tion, and that the C-terminus of SKIP is required to maintain the stability of the protein in plant cells. Together, our findings demonstrate the structural domain organization of SKIP and reveal the core domains and motifs underlying SKIP function in plants.

关键词： SKIP spliceosome Pafl complex core domain and motif nuclear localization signal 26Sproteasome-mediated degradation

Effect of NaCl concentration on the Cumulative Strain and Pore Distribution of Clay under Cyclic Loading

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Fluid Dynamics & Materials Processing 2024年第2期20卷 447-461页

作者： Xinshan Zhuang Shunlei xia Ruijie Pan School of Civil Engineering and Architecture Hubei University of TechnologyWuhan430068China

Clay,as the most common soil used for foundationﬁll,is widely used in various infrastructure *** phy-sical and mechanical properties of clay are inﬂuenced by the pore solution *** study uses a GDS static/dynamic triaxial apparatus and nuclear magnetic resonance experiments to investigate the effects of cyclic loading on clay ***,the development of cumulative strain in clay is analyzed,and aﬁtting model for cumulative plastic strain is introduced by considering factors such as NaCl solution concentration,con-solidation stress ratio,and cycle *** particular,the effects of the NaCl solution concentration and con-solidation stress ratio on the pore distribution of the test samples before and after cyclic loading are examined,and the relationship between microscopic pore size and macroscopic cumulative strain is obtained *** results show that as the consolidation stress ratio grows,an increasing number of large pores in the soil samples are transformed into small *** the NaCl solution concentration becomes higher,the number of small pores gradually decreases,while the number of large pores remains *** loading causes the disappearance of the large pores in the samples,and the average pore size before cyclic loading is posi-tively correlated with the axial cumulative strain after cyclic *** cumulative strain produced by the soil under cyclic loading is inversely proportional to the NaCl solution concentration and consolidation stress ratio.

关键词： Geotechnical engineering clay cyclic loading nuclear magnetic resonance NaCl solution consolidation ratio accumulative strain

A single-cell transcriptome atlas reveals the trajectory of early cell fate transition during callus induction in Arabidopsis

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Plant Communications 2024年第8期5卷 18-31页

作者： Ruilian Yin Ruiying Chen Keke xia Xun Xu College of Life Sciences University of Chinese Academy of SciencesBeijing 10049China BGI Research Beijing 102601China Guangdong Provincial Key Laboratory of Genome Read and Write BGI-ShenzhenShenzhen 518120GuangdongChina

The acquisition of pluripotent callus from somatic cells plays an important role in plant development studies and crop genetic *** developmental process incorporates a series of cell fate transitions and ***,our understanding of cell heterogeneity and mechanisms of cell fate transition during callus induction remains quite ***,we report a time-series single-cell transcriptome experiment on Arabidopsis root explants that were induced in callus induction medium for 0,1,and 4 days,and the construction of a detailed single-cell transcriptional atlas of the callus induction *** identify the cell types responsible for initiating the early callus:lateral root primordium-initiating(LRPI)-like cells and quiescent center(QC)-like ***-like cells are derived from xylem pole pericycle cells and are similar to lateral root *** delineate the developmental trajectory of the dedifferentiation of LRPI-like cells into QC-like ***-like cells are undifferentiated pluripotent acquired cells that appear in the early stages of callus formation and play a critical role in later callus development and organ *** also identify the transcription factors that regulate QC-like cells and the gene expression signatures that are related to cell fate de***,our cell-lineage transcriptome atlas for callus induction provides a distinct perspective on cell fate transitions during callus formation,significantly improving our understanding of callus formation.

关键词： callus induction single-cell RNA-seq cell fate transition developmental trajectory transcriptional regulation pluripotency

Strain‑Induced Surface Interface Dual Polarization constructs PML‑Cu/Bi_(12)O_(17)Br_(2) High‑Density Active Sites for CO_(2) Photoreduction

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Nano-Micro Letters 2024年第5期16卷 169-183页

作者： Yi Zhang Fangyu Guo Jun Di Keke Wang Molly Meng‑Jung Li Jiayu Dai Yuanbin She Jiexiang xia Huaming Li School of Chemistry and Chemical Engineering Institute for Energy ResearchSchool of the Environment and Safety EngineeringJiangsu UniversityZhenjiang 212013People’s Republic of China State Key Laboratory Breeding Base of Green Chemistry‑Synthesis Technology College of Chemical EngineeringZhejiang University of TechnologyHangzhou 310014People’s Republic of China College of Science and Hunan Key Laboratory of Extreme Matter and ApplicationsNational University of Defense TechnologyChangsha 410073People’s Republic of China Department of Applied Physics The Hong Kong Polytechnic UniversityHung HomHong KongPeople’s Republic of China School of Chemistry and Chemical Engineering National Special Superfine Powder Engineering Research CenterNanjing University of Science and TechnologyNanjing 210094People’s Republic of China Key Laboratory of the Ministry of Education for Advanced Catalysis Materials Zhejiang Normal UniversityJinhua 321004People’s Republic of China

The insufficient active sites and slow interfacial charge trans-fer of photocatalysts restrict the efficiency of CO_(2) *** synchronized modulation of the above key issues is demanding and ***,strain-induced strategy is developed to construct the Bi–O-bonded interface in Cu porphyrin-based monoatomic layer(PML-Cu)and Bi_(12)O_(17)Br_(2)(BOB),which triggers the surface interface dual polarization of PML-Cu/BOB(PBOB).In this multi-step polarization,the built-in electric field formed between the interfaces induces the electron transfer from con-duction band(CB)of BOB to CB of PML-Cu and suppresses its reverse ***,the surface polarization of PML-Cu further promotes the electron converge in Cu *** introduction of PML-Cu endows a high density of dispersed Cu active sites on the surface of PBOB,significantly promoting the adsorption and activation of CO_(2) and CO *** conversion rate of CO_(2) photoreduction to CO for PBOB can reach 584.3μmol g-1,which is 7.83 times higher than BOB and 20.01 times than *** work offers valuable insights into multi-step polarization regulation and active site design for catalysts.

关键词： Bi_(12)O_(17)Br_(2) Porphyrin CO_(2)photoreduction Polarization Active sites

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Rosemary leaf powder improves egg quality, antioxidant status, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens

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Animal Nutrition 2024年第2期17卷 325-334页

作者： Lianhua Zhang Junwei Ge Fei Gao Min Yang Hui Li Fei xia Hongtong Bai xiangshu Piao Zhiying Sun Lei Shi Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences China National Botanical Garden State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences College of Pharmacy Shandong University of Traditional Chinese Medicine University of Chinese Academy of Sciences Beijing Jingwa Agricultural Science&Technology Innovation Center State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University

his study sought to determine the effects of rosemary leaf powder(RP) on laying performance, egg quality, serum indices, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens. A total of 84 “Jing Tint 6” laying hens at 65-week old were randomly divided into 2 groups and fed either a basal diet(con) or a basal diet supplemented with 0.3% RP. Our study revealed that RP improved the Haugh unit and decreased yolk n-6/n-3 polyunsaturated fatty acid(PUFA) ratio of laying hens,increased serum superoxide dismutase(SOD), jejunal activities of SOD and catalase(CAT), and jejunal zonula occludens-1(ZO-1) expression, as well as decreased serum tumor necrosis factor-a(TNF-a) level and jejunal TNF-a m RNA expression. Rosemary leaf powder markedly enhanced(P concentration, and reduced(P considered a promising feed additive to promote the production performance of late-phase laying hens.

关键词： Rosemary leaf powder Production Antioxidant status Gut health Microbial community Laying hen

Machine learning and bioinformatics to identify biomarkers in response to Burkholderia pseudomallei infection in mice

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BIOCELL 2024年第4期48卷 613-621页

作者： YAO FANG FEI xia FEIFEI TIAN L EI QU FANG YANG JUAN FANG ZHENHONG HU HAICHAO LIU Department of Respiratory and Critical Care Medicine General Hospital of Center Theater of PLAWuhan430070China School of Medicine Wuhan University of Science and TechnologyWuhan430065China School of Life Science and Engineering Southwest Jiaotong UniversityChengdu611756China

Objective:In the realm of Class I pathogens,Burkholderia pseudomallei(BP)stands out for its propensity to induce severe *** the intricate interactions between BP and host cells is imperative for comprehending the dynamics of BP infection and discerning biomarkers indicative of the host cell response ***:mRNA extraction from BP-infected mouse macrophages constituted the initial step of our *** gene expression arrays,the extracted RNA underwent conversion into digital *** percentile shift method facilitated data processing,with the identiﬁcation of genes manifesting signiﬁcant differences accomplished through the application of the ***,a comprehensive analysis involving Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway was conducted on the differentially expressed genes(DEGs).Leveraging the ESTIMATE algorithm,gene signatures were utilized to compute risk scores for gene expression *** vector machine analysis and gene enrichment scores were instrumental in establishing correlations between biomarkers and macrophages,followed by an evaluation of the predictive power of the identiﬁed ***:The functional and pathway associations of the DEGs predominantly centered around G protein-coupled receptors.A noteworthy positive correlation emerged between the blue module,consisting of 416 genes,and the ***4,identiﬁed through support vector machine analysis among intersecting genes,indicated a robust interaction with macrophages,suggesting its potential as a robust ***4 exhibited commendable predictive efﬁcacy,with BP infection inducing its expression in both macrophages and mouse lung *** blotting in macrophages conﬁrmed a signiﬁcant upregulation of FZD4 expression from 0.5 to 24 h *** mouse lung tissue,FZD4 manifested higher expression in the cytoplasm of pulmonary epithelial cells in BP-infected lungs than in the

关键词： Burkholderia pseudomallei Microarray assay Machine learning Bioinformatics FZD4

Investigation of the Cytotoxicity of CAR-NK-92 Cells Targeting CEA Against Gastric Cancer Cells

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Journal of Clinical and Nursing Research 2024年第6期8卷 410-416页

作者： Xinyu Zheng xiaoxiao Zhou Xingzhou xia xiaomeng Chen Department of Gastroenterology The Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Objective:To construct CAR-NK-92 cells targeting carcinoembryonic antigen(CEA)and study their killing effect on gastric cancer ***:CAR-NK-92 cells targeting CEA were *** co-culturing CAR-NK-92 cells with MKN-45 gastric cancer cells,the killing effect of CAR-NK-92 cells was detected by a lactate dehydrogenase release *** secretion levels of gamma interferon and granulocyte-macrophage colony-stimulating factor were measured using an ELISA ***:The lactate dehydrogenase release assay showed that CAR-NK-92 cells had a significant killing effect on MKN-45 cells compared to con-NK-92 cells,and the difference was statistically significant(Pconclusion:CAR-NK-92 cells targeting CEA exhibit a significant killing effect on CEA-positive gastric cancer cells.

关键词： Gastric cancer Natural killer cell Carcinoembryonic antigen Immunotherapy

Targeted Therapy of CEA-CAR-NK Cells Against Colorectal Cancer Cells

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Proceedings of Anticancer Research 2024年第4期8卷 13-19页

作者： Xinyu Zheng xiaomeng Chen Xingzhou xia Wenzhen Wang Qian Liu Department of Gastroenterology The Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Objective:Investigate the cytotoxic effect of CAR-NK cells targeting CEA on colorectal cancer cells with positive CEA ***:The mRNA and protein levels of CEA in different CRC cell lines were detected by qRT-PCR and Western blot *** transduction was used to construct CAR-NK cells and empty vector con-NK cells targeting *** microscopy and WB were used to determine whether the cells successfully constructed and expressed CAR *** effector NK cells were co-cultured with target cells,and the levels of LDH,IFN-γ,and GM-CSF were *** killing rate of effector cells was calculated,and the release of cytokines during the killing of target cells by different effector cells was ***:The expression level of CEA in colorectal cancer patients was significantly higher than that in normal samples and other tumor samples,and the prognosis survival time of patients with high CEA expression was lower than that of CRC patients with low or no CEA expression(Pcon-NK92 cells expressed green fluorescence under a microscope,and WB results showed that CEA-CAR-NK92 cells successfully expressed the CAR *** with con-NK92 cells and NK92 cells,CEA-CAR-NK92 cells effectively killed HT29 cells(Pcon-NK92 cells and NK92 cells was not significant(Pconclusion:CAR-NK92 cells targeting CEA can effectively kill CEA-positive colorectal cancer cells.

关键词： Colorectal cancer Chimeric antigen receptor Natural killer cells Carcinoembryonic antigen Immunotherapy

基于图拉普拉斯正则化的PET图像核重建方法

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电子学报 2024年第1期52卷 118-128页

作者：盛玉霞孙坤柴利武汉科技大学信息科学与工程学院湖北武汉430081 浙江大学控制科学与工程学院浙江杭州310027

正电子发射断层成像(Positron Emission Tomography,PET)在很多疾病的早期诊断中有重要的作用,PET图像重建的难点之一是如何在保持重建图像中病灶边缘特性的同时具有良好的去噪性能.针对此问题,本文提出了一种结合图拉普拉斯正则化和深... 详细信息

正电子发射断层成像(Positron Emission Tomography,PET)在很多疾病的早期诊断中有重要的作用,PET图像重建的难点之一是如何在保持重建图像中病灶边缘特性的同时具有良好的去噪性能.针对此问题,本文提出了一种结合图拉普拉斯正则化和深度图像先验的PET图像核重建方法 .设计了改进的U-net神经网络,将PET前向投影模型中的核系数表示为神经网络的输出;通过先验图像构建图拉普拉斯矩阵,重建问题被建模为基于神经网络的带图拉普拉斯正则化项的最大似然函数优化问题.利用优化转移方法导出了收敛的迭代重建算法,每一次迭代包括由核重建方法更新图像和利用神经网络更新核系数两个步骤.仿真和临床实验结果表明,本文提出的方法在不同的指标下都有更好的重建效果,优于已有核重建方法以及最新的基于深度系数先验的重建方法 .

关键词： PET 图像重建核方法深度图像先验图拉普拉斯正则化

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离子色谱法测定人血白蛋白制品中辛酸钠的含量

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药物分析杂志 2024年第2期44卷 249-255页

作者：严翠霞史芳亮韩春霞方欣欣郑璐侠邵泓上海市食品药品检验研究院国家药监局治疗类单抗质量控制重点实验室上海201203 赛默飞世尔科技(中国)有限公司上海201203

目的:建立离子色谱法测定人血白蛋白制品中辛酸钠的含量。方法:样品加淋洗液[甲醇-乙腈^(-1).0 mmol·L^(-1)盐酸(20∶42∶38)]沉淀蛋白,离心取上清液,过滤后直接进样,以庚酸为内标。采用Dionex InPacTMNS1分析柱(250 mm×4 mm, 10μm)... 详细信息

目的:建立离子色谱法测定人血白蛋白制品中辛酸钠的含量。方法:样品加淋洗液[甲醇-乙腈^(-1).0 mmol·L^(-1)盐酸(20∶42∶38)]沉淀蛋白,离心取上清液,过滤后直接进样,以庚酸为内标。采用Dionex InPacTMNS1分析柱(250 mm×4 mm, 10μm)与Dionex InPacTMNG1保护柱(35 mm×4 mm, 10μm),流速1.0 mL·min^(-1),ASRS 300膜抑制器,化学抑制,再生液为5 mmol·L^(-1)四丁基氢氧化钠溶液,电导检测器检测,柱温30℃,进样量25μL。结果:辛酸峰与内标峰间的分离度>1.5;辛酸钠在0.38~2.52 mmol·L^(-1)范围内线性关系良好,r=0.999 5(n=6);重复性试验的RSD为1.1%(n=6);平均加样回收率为97.4%,RSD为1.8%(n=9);定量限与检测限分别为0.19 nmol与0.09 nmol;国内外7家企业20批人血白蛋白制品中辛酸钠含量范围为0.073~0.163 mmol·g^(-1)。结论:本研究建立的方法操作简便易行,结果准确,灵敏度高,重复性好,可用于人血白蛋白制品中辛酸钠含量的测定,为其质量控制提供方法保证。

关键词：离子色谱法内标法人血白蛋白辛酸钠质量控制

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