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The Acute Toxicity Test of Neo-enfx in Mice

饲料工业 2011年第13期 28-29页

作者： college of veterinary medicine of nanjing agricultural university college of veterinary medicine of nanjing agricultural university

Enrofloxacin, a third-generation quinolone, is a broad-spectrum antibacterial drug. It has activity against some bacteria, which include ***, klebsiella, salmonella, vibrio parahaemolyticus, staphylococcus aureus, pse... 详细信息

关键词： The Acute Toxicity Test of Neo-enfx in Mice Test oral

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Effects of heat stress at an early age on various blood and meat quality parameters in broiler chickens

Effects of heat stress at an early age on various blood and ...

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中国畜牧兽医学会兽医病理学分会第十五次、中国病理生理学会动物病生专业委员会第十四次学术讨论会

作者： Jimian,Yu Endong,Bao (college of veterinary medicine,nanjing agricultural university,nanjing China 210095)

The objective of this study was to investigate the effects of varying periods of acute ante-mortem heat stress on meat quality attributes and selected serum metabolites of Arbor Acre (AA) broiler *** AA broilers (n=120) were randomly divided into 6 groups of 20 birds per *** 30 days of adaptive feeding at ambient temperature,100 experimental broilers were suddenly heat stressed by

关键词： heat stress meat quality broiler chickens

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Screening of Key Factors Related to Lipids Metabolism in mbryonic Liver of Broiler Chickens with Different Fat Deposition by Comparative Proteomics Strategy

Screening of Key Factors Related to Lipids Metabolism in mbr...

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全国动物生理生化第十次学术交流会

作者： Huang Jianzhen Ma Haitian Zou Sixiang college of veterinary medicine,nanjing agricultural university

In order to investigate the difference of fat metabolism during embryonic development,a comparative proteomics strategy was applied and AA broiler and Sanhuang broiler chickens with different growth and fat deposition...

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Expression and Localization of HSPs in the Liver of Transported Pigs

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agricultural Sciences in China 2002年第6期1卷 690-695页

作者： BAO En-dong, K. R. Sultan, B. Nowak and J. Hartung( college of veterinary medicine, nanjing agricultural university, nanjing 210095 , P.R.China Department of the Science of Food of Animal Origin , Faculty of veterinary medicine, university of Utrecht, TheNetherlands Institute of Meat Hygiene and Meat Technology, Hannover veterinary School, Hannover, Germany Institute of Animal Hygiene and Animal Welfare, Hannover veterinary School, Hannover, Germany) college of veterinary medicine Nanjing Agricultural University Nanjing 210095 P. R . China Department of the Science of Food of Animal Origin Faculty of Veterinary Medicine University of Utrecht The Netherlands Institute of Meat Hygiene and Meat Technology Hannover Veterinary School Hannover Germany Institute of Animal Hygiene and Animal Welfare Hannover Veterinary School Hannover Germany

To demonstrate the distribution and expression of heat shock proteins (HSPs) in the liver, five kinds of HSPs, namely HSP70, HSP72, HSP86, HSP90 and HSP27 were determined after a long distance transport using immunohistochemistry and Western blot. All five HSPs were regularly detected in the liver of both the transported and the control group. Both nuclear and cytoplasmic labeling was present in hepatic cells; however, localization of HSPs was quite different in hepatocytes. The majority of HSP70, HSP72 and HSP27 appeared to predominate in cytoplasm of the hepatocytes. Nuclear signals of HSP90 and HSP86 were most prominent in hepatocytes in which immunostaining in the cytoplasm was relatively weak compared with that in the nucleus. There was subjectively lower staining in the edge of hepatic labule, and lower numbers of HSPs labeled hepatic cells corresponded to light microscopic lesions. The obvious inductions of some HSPs were detected in the liver, especially the HSP90(P<0.01) which belongs to the HSP90 family. However, the obvious reductions of HSP86(P<0.05), which belong to the HSP90 family, and HSP27 (P <0.05), which belong to small families, were detected in stressed pig liver after six hours of long distance transportation.

关键词： Pig Transport Stress Heat shock protein Liver

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马立克氏病胚胎免疫雏鸡体重和免疫器官增重变化

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畜牧与兽医 1998年第1期30卷 4-5页

作者：鲍恩东南京农业大学动物医学院

１８日龄鸡胚免疫接种火鸡疱疹病毒（ＨＶＴ）疫苗后，对出壳后雏鸡及其用马立克氏病（ＭＤ）病毒攻毒鸡体重与免疫器官增长效果的对比研究表明：相同饲养条件下胚胎免疫雏鸡的增长速度高于非免疫对照鸡，而且胚胎免疫组雏鸡免疫器官的... 详细信息

１８日龄鸡胚免疫接种火鸡疱疹病毒（ＨＶＴ）疫苗后，对出壳后雏鸡及其用马立克氏病（ＭＤ）病毒攻毒鸡体重与免疫器官增长效果的对比研究表明：相同饲养条件下胚胎免疫雏鸡的增长速度高于非免疫对照鸡，而且胚胎免疫组雏鸡免疫器官的生长发育普遍较相应的对照组雏鸡快。

关键词：马立克氏病胚胎免疫生长发育增重率

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Expression pattern of NMS/NMU2R mRNA in the pig reproductive axis during the estrus cycle and the role of NMS on the reproductive axis in vitro

Expression pattern of NMS/NMU2R mRNA in the pig reproductive...

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： Guihong Yang,Zhihai Lei~* college of veterinary medicine,nanjing agricultural university,nanjing 210095,Jiangsu,P R China

Evidence has revealed that neuromedin S(NMS) and neuromedin U receptor type-2(NMU2R) mRNAs are expressed in the central nervous system and reproductive *** data indicated that variation of NMS and NMU2R was due to the phases of the adult rat hypothalamus estrus ***, the expression and function of NMS in the pig reproductive axis remains *** this study,16 virginal gilts were classified into four groups:proestrus,estrus,diestrus 1,and diestrus 2;the expression of NMS and NMU2R in the cyclic pig hypothalamus-pituitary-ovary axis was studied by reverse transcriptaion-polymerase chain reaction(RT-PCR),and the effect of NMS on the reproductive axis in vitro was detected by radioimmunoassay(RIA).The cloned pig NMS and NMU2R sequences were 82%and 90.2%identical to those of the corresponding human homologues,***-PCR showed that NMS and NMU2R mRNA expression in the hypothalamus and pituitary changed with the estrus cycle,i.e.,with the highest level in the proestrus group and the lowest in the estrus *** the ovary,NMS and NMU2R expression was highest in the diestrus 2 group and the lowest in the proestrus *** the in vitro study, different concentrations of NMS induced the release of gonadotropin-releasing hormone,luteinizing hormone,and estradiol at different levels of the reproductive *** together,the expression pattern of NMS during the estrus cycle and its role in reproductive hormones in vitro provide novel evidences of the potential roles of NMS in the regulation of pig reproduction.

关键词： NMS NMU2R RT-PCR Radioimmunoassay Reproductive axis

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Identification of putative virulent genes by SSH in Streptococcus equi ssp. zooepidemicus

Identification of putative virulent genes by SSH in Streptoc...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： MA Zhe,FAN Hong-jie~*,LU Cheng-ping (college of veterinary medicine,nanjing agricultural university,nanjing 210095)

Suppression subtractive hybridization(SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ***(SEZ).There were fourteen genomic regions that were only present in virulent strain *** regions encoded 14 putative proteins,some of which were homologous to proteins associated with cellular surface structure,molecular synthesis,energy metabolism,regulation,transport systems and others of unknown *** for 6 particular regions were designed from the already published SEZ sequence. We then used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources,regions,groups and *** results showed that these 6 DNA fragments were widely distributed in SEZ strains,yet they were not existence in the avirulent strain ***,these fragments could not be detected in other Streptococcus groups.

关键词： Streptococcus equi ssp zooepidemicus Suppression subtractive hybridization Putative virulent genes PCR detection

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Pulmonary Arterial Pressure and Electrocardiograms in Broiler Chickens Infused Intravenously with L-NAME,an Inhibitor of Nitric Oxide Synthase,or Sodium Niroprusside(SNP),a-Nitric Oxide Donor

Pulmonary Arterial Pressure and Electrocardiograms in Broile...

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中国畜牧兽医学会兽医病理学分会第十一次兽医病理学、第十次动物病理生理学学术研讨会

作者： Sun Weidong,Wang Xiaolong, Wang Jingyong, and Xiang Ruiping college of veterinary medicine, nanjing agricultural university,nanjing,210095, China

Broilers were divided at 42 to 44 days of age into a Control group （n=10） and a Treatmentgroup（n=30）.The mean pulmonary arterial pressure （mPAP） and electrocardiogram（ECG） Leads Ⅱand aV. were measured 1,3,and 4 hours after an intravenous injection of 0 .9 % saline （Control group）or N- nitro -L-arginine methyl esther（L-NAME）, an inhibitor of nitric oxide synthase and thus aninhibitor of endothelial nitric oxide（ON）peduction（Treatment group）. At 1 and 2 hours but not 4 hourspost-injection,L-NAME significantly increased the mPAP andthe amplitudes of the ECG S and RSwaves Leads Ⅱ and aV when compared with conrol *** correlation coefficient between themPAP and the ECG and RS wave amplitudes for Lead Ⅱ within the Treatment group were -0. 848 and-0. 553 at 1 hour, and -0. 789 and -0 .512 at 2 hour, respectively. The corresponding coefficients forLead aV were -0. 735, -0. 596, -0. 663, and -0. 724, respectively. After suitable mPAP andECG values had been recorded at each time interval,sodium nitroprusside （SNP）,which acts as a short-lived NO donor molecule,was injected intravenously via a right -cardiac catheter. Within 5 minutesafter the SNP injection, the mPAP and the ECG Lead Ⅱ Sand RS wave amplitudes were transiently re-duced to levels that,at 1 and 2 hours after L-NAME injection, did not differ from Control values. With-in 10 minutes after the SNP injection, all values returned to the levels previously induced by L - *** results demonstrate that L-NAME increased the myocardialcontractility and PAP, whereas SNPtransiently reversed the effects of L-NAME on myocardial contracility and PAP. It appears likely fromthese results that the pulmonary vascular endothelium releases NO that in tum reduces the pulmonaryvascular resistance or attenuates myocardial contractility in broiler chickens.

关键词： pulmonary arterial pressure,broiler chickens, electrocardiogram, L - NAME,SNP

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Conservation of Osteoprotegerin (OPG) in Chicken

Conservation of Osteoprotegerin (OPG) in Chicken

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国际家禽营养代谢病研讨会

作者： Jing Yao, Jian-Feng Zhang, Jia-Fa Hou (college of veterinary medicine of nanjing agricultural university, nanjing 210095, China)

One of the most recently identified members of the tumor necrosis factor receptor (TNFR) family, Osteoprotegerin (OPG) is a heparin-binding secretory glycoprotein and has been shown to specifically inhibit osteoclast development. In this study, the chicken (ch) OPG gene had now been amplified from the osteoblasts cultured in vitro, which was 1.2-kb long and capable to encode a -45kDa protein and had 99.3% identical to the sequence reported in Genbank. By analysis, the chicken OPG protein was 68.76%, 68.60% and 68.29% homologous to human, rat and mouse, respectively, which suggested that the OPG gene had a high degree of conservation in evolution.

关键词： conservation osteoprotegerin chicken

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Preparation of Selenium-Zinc-Enriched Probiotics and Their Effect on Blood Selenium and Zinc Concentrations,Antioxidant Capacities and Intestinal Microflora in Canine

Preparation of Selenium-Zinc-Enriched Probiotics and Their E...

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中国畜牧兽医学会2010年学术年会——第二届中国兽医临床大会

作者： Zhihua REN,Zhiping ZHAO,Yangguang WANG,AND Kehe HUANG~* college of veterinary medicine,nanjing agricultural university,nanjing 210095,China

The aim of this study was to prepare Se-Zn-enriched probiotics and investigate their effect on blood Se and Zn concentrations,blood antioxidant capacities,and intestinal microflora in *** preparation was performed in a single-factor *** optimal culture conditions were as follows:the initial concentrations of Se and Zn were 5μag/ml and 150μg/ml respectively;the inoculation volume was 5%;and the liquid volume of the medium was 50 ml in a 250-ml flask. The medium was then cultured at 32℃for 36 *** biomass of the final product was 26.83 g/l,organic-Se concentration was 173.35μg/g,organic-Zn concentration was 4.38 rag/g,Candida utilis biomass was 8.69 lg colony-forming units(CFU)/ml,and Lactobacillus biomass was 9.61 lg CFU/*** vivo 20 indigenous dogs were randomly assigned to two dietary treatment groups for a 35-day study and fed a basal diet or diet supplemented with 2.0 g of Se-Zn-enriched *** and fecal samples were collected on days 0,15,and 30 of the *** with the control group,the blood Se concentration,the blood Zn concentration,the activities of glutathione peroxidase,superoxide dismutase,and the total antioxidant capacity in the blood and the amounts of Lactobacillus and Bifidobacterium in the feces increased in the supplemented group during the period of supplementation(P < 0.01),while the malondialdehyde level in the blood and the amounts of Escherichia coli,Staphylococcus and Enterococcus in the feces decreased in the supplemented group(P < 0.01).

关键词： Preparation Selenium-Zinc-enriched probiotics Blood selenium concentration Blood zinc concentration Antioxidation Intestinal microflora Canine

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