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The role of window Ca2+ current in hypothermic Ca2+ overload in non-hibernators

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The role of window Ca2+ current in hypothermic Ca2+ overload...

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Xiao-Chen LI,Xue-Mei HAO,Shi-Qiang WANG State Key Laboratory of Biomembrane and Membrane Biotechnology,college of life sciences,peking university,beijing 100871,china

While hibernating mammals are able to regulate their body temperature between 37℃and the freezing point,non-hibernating mammals,including humans,could not survive hypothermia lower than～20℃.We found that low temperature results in intracellular calcium overload and incomplete relaxation as indicated by the marked increase in the resting tension of myocardium in non-hibernating *** of nifedipine,an inhibitor of L-type calcium channels,could reverse the effect of cooling,suggesting that the calcium influx(I) could be a determinant of the hypothermia-induced intracellular calcium *** test this hypothesis,we compared the temperature-dependence of I between the rat,a nonhibernating mammal,and the ground squirrel(Citellus dauricus), a *** the temperature was lowered,I declined significantly in both rats and ground squirrels, without changing the reversal *** activation curve showed that the I in rats exhibited a more negative half-activation potential(V) than in ground *** the temperature was lowered,the V in rats shifted even further to the left,whereas that in ground squirrels to the opposite *** of the availability curves showed that the half-inactivation potential(V) also changed distinctly in two *** the result,the window current of I was much larger,and increased dramatically with lowing temperature in rats,but not in round *** increased window current in rats would lead to continuous calcium influx in the background of reduced calcium handling capacity at the low temperature,and thus is a key reason for the hypothermia-induce calcium overload and cardiac dysfunction in non-hibernating *** the adaptive mechanisms of hibernating models would provide new ideas for cryo-medicine and organ preservation.

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A draft sequence of the rice (Oryza sativa ssp. indica) genome

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Chinese Science Bulletin 2001年第23期46卷 1937-1942页

作者： YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG Huanmingbeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of sciences, beijing 101300, china Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang university/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, china Institute of Genetics, Chinese Academy of sciences, beijing 100101, china National Hybrid Rice R & D Center, Changsha 410125, china Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of sciences, beijing 100101, china college of life sciences, peking university, beijing 100871, china Institute of Theoretical Physics, Chinese Academy of sciences, beijing 1Q0080, china Digital china Ltd., beijing 100080, china Institute of Computing Technology, Chinese Academy of sciences, beijing 100080, china Medical college, Xi’an Jiaotong university, Xi’an 710061, chinaThese authors contributed equally to this work.Corresponding author.Corresponden beijing Genomics Institute/Center of Genomics & Bioinformatics Chinese Academy of Sciences Beijing China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang university/Key Laboratory of Bioinformatics of Zhejiang Province Hangzhou China college of life sciences Peking University Beijing China Medical college Xi’an Jiaotong University Xi’an China Institute of Genetics Chinese Academy of Sciences Beijing China Laboratory of Bioinformatics Institute of Biophysics Chinese Academy of Sciences Beijing China National Hybrid Rice R & D Center Changsha China Institute of Theoretical Physics Chinese Academy of Sciences Beijing China Digital china Ltd. Beijing China Institute of Computing Technology Chinese Academy of Sciences Beijing China

The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in china and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package

关键词： rice genome draft sequences.

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利用竞争型ELISA法鉴定硒诱导的金属硫蛋白

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生物化学与生物物理学报 2002年第2期34卷 245-247页

作者：刘颖江冠群茹炳根徐卓立郭军华北京大学生命科学学院蛋白质工程国家重点实验室北京军医学院北京北太平路医院临床药理基地北京100871 北京100071 北京100039

利用竞争型ELISA法鉴定硒诱导的金属硫蛋白 (MT) ,发现对照组小鼠肝脏MT含量为 (2 .47± 0 .90 )μg/g湿重组织 ,硫酸锌组小鼠肝脏MT含量为 (8.15± 2 .2 0 ) μg/ g湿重组织 ,硒麦芽组小鼠肝脏MT含量为(12 .80± 1.44 ) μg/ g湿重组... 详细信息

利用竞争型ELISA法鉴定硒诱导的金属硫蛋白 (MT) ,发现对照组小鼠肝脏MT含量为 (2 .47± 0 .90 )μg/g湿重组织 ,硫酸锌组小鼠肝脏MT含量为 (8.15± 2 .2 0 ) μg/ g湿重组织 ,硒麦芽组小鼠肝脏MT含量为(12 .80± 1.44 ) μg/ g湿重组织。锌组和硒组的MT含量与对照组相比有显著性差异 (P <0 .0 5 ,P <0 .0 5 )。硒组MT含量要显著高于锌组的MT含量 (P <0 .0 5 )。

关键词：硒金属硫蛋白竞争型ELISA

Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis

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Cell Research 2007年第5期17卷 471-482页

作者： Genji Qin1 Hongya Gu1,2 Ligeng Ma1,3 Yiben Peng1 Xing Wang Deng1,3 Zhangliang Chen1 Li-Jia Qu1,2 1 peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory for Protein Engineering and Plant Genetic Engineering, college of life sciences, peking university, beijing 100871, china 2 The National Plant Gene Research Center (beijing), beijing 100101, china 3 Department of Molecular, Cellular, and Developmental Biology, Yale university, New Haven Connecticut 62520-8104, USA peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology National Laboratory for Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing China The National Plant Gene Research Center (beijing) Beijing China Department of Molecular Cellular and Developmental Biology Yale University New Haven Connecticut USA

Carotenoids play an important role in many physiological processes in plants and the phytoene desaturase gene （PDS3）encodes one of the important enzymes in the carotenoid biosynthesis pathway. Here we report the identification andanalysis of a T-DNA insertion mutant of PDS3 gene. Functional complementation confirmed that both the albino anddwarf phenotypes of the pds3 mutant resulted from functional disruption of the PDS3 gene. Chloroplast development wasarrested at the proplastid stage in the pds3 mutant. Further analysis showed that high level of phytoene was accumulatedin the pds3 mutant. Addition of exogenous GA3could partially rescue the dwarf phenotype, suggesting that the dwarfphenotype of the pds3 mutant might be due to GA deficiency. Microarray and RT-PCR analysis showed that disruptingPDS3 gene resulted in gene expression changes involved in at least 20 metabolic pathways, including the inhibition ofmany genes in carotenoid, chlorophyll, and GA biosynthesis pathways. Our data suggest that the accumulated phytoenein the pds3 mutant might play an important role in certain negative feedbacks to affect gene expression of diverse cel-lular pathways.

关键词： Arabidopsis thaliana phytoene desaturase albino dwarf microarray analysis

The over-expression of AtTCP5 leads to the defected leaf development through suppressing cell proliferation and endoreduplication in Arabidopsis

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The over-expression of AtTCP5 leads to the defected leaf dev...

2006年拟南芥研究学术研讨会

作者： Huayan Zhao, Qing Tao, Dongxue Hu, Hongya Gu, Li-Jia Qu National Laboratory for Protein Engineering and Plant Genetic Engineering, college of life sciences, peking university, beijing 100871, china

＜正＞The TCP gene family plays important roles in leaf development. One of the family members in Arabidopsis, AtTCP5, shows high sequence similarity with CINCINNATA, a leaf curvature-related protein from snapdragon. Expression pattern analysis showed that AtTCP5, highly expressed in leaves, was expressed precisely at the onset of cell differentiation and expressed abundantly in the area that is

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Statistical analysis on adaptive evolution of SQUA genes in angiosperms

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Progress in Natural Science:Materials International 2005年第1期 94-97页

作者： CHEN Yongyan 1,2, ZHONG Yang 3*, TIAN Bo 1, YANG Ji 4 and LI Dezhu 1*(1. Laboratory of Plant Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of sciences, Kunming 650204, china 2. college of Bioengineering, Dalian university, Dalian 116622, china 3. Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, School of life Science, Fudan university, Shanghai 200433, china 4. college of life sciences, peking university, beijing 100871, china) Laboratory of Plant Biodiversity and Biogeography，Kunming Institute of Botany，Chinese Academy of sciences，Kunming 650204 China college of Bioengineering，Dalian university，Dalian 116622，china Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering，School of life Science，Fudan university，Shanghai 200433，china Laboratory of Plant Biodiversity and Biogeography，Kunming Institute of Botany，Chinese Academy of sciences，Kunming 650204 China college of life sciences，peking university，beijing 100871，china

SQUAMOSA (SQUA) subfamily includes important perianth identity genes of MADS-box gene family. SQUA genes of Dendrocalamus latiflorus were sequenced, and phylogenetic form on SQUA genes in angiosperms was analyzed. Relative rate and adaptive evolution after SQUA gene duplication in recent common ancestor of monocots and eudicots were analyzed using the methods of relative rate test, statistic on synonymous and non-synonymous coden substitution sites and likelihood rate test. The results show that both of relative rate and synonymous and non-synonymous coden substitution in eudicot clade are significantly higher than those in monocot clade, and the value of d N/d S uncovered possible positive selective pressure in eudicot clade.

关键词： angiosperms Dendrocalamus latiflorus SQUA gene relative rate test likelihood rate test adaptive evolution.

Hemichannels or gap junctions contributed to neuronal ischemic tolerance induced by astrocyte-conditioned medium

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Hemichannels or gap junctions contributed to neuronal ischem...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Juan-Juan ZHAO,Jun-Zhan JING,Qing-Yang XIAO,Shan GAO,Zhen CHAI~* State Key Laboratory of Biomembrane and Membrane Biotechnology,college of life sciences,peking university, beijing 100871,china

＜正＞As a main subtype of glia in the central nervous system,astrocytes have diverse and comprehensive functions,from nutrient providing to synaptic transmission *** play protective roles in ischemia injury by taking up glutamate,releasing neurotrophic factors,and so ***,the function of gap junctions in this process is still *** cortical neurons were treated with 3-h oxygen-glucose deprivation（OGD） to induce cell *** found that astrocyte-conditioned medium（ACM） could reduce neuronal death from -95%to～30%,as indicated by MTT *** centrifugal filter devices,the molecular weight of protective factors released from astrocytes was speculated to be＜3 *** protection of ACM was remarkably decreased when 50μmol/L gap junction and hemichannel blocker carbenoxolone（CBX） was applied to the cultured astrocytes before ACM collection. Furthermore,ACM pretreatment raised neuronal level of reductive glutathione（GSH） and decreased reactive oxygen species（ROS） content,while ACM pretreated with CBX could block GSH increase in *** results suggested that astrocytes enhanced neuronal tolerance against OGD injury by releasing protective factors dependent on hemichannels or gap junctions.

关键词： astrocyte-conditioned medium cortical neuron astrocyte oxygen-glucose deprivation GSH

凝胶色谱柱复性在低分子量尿激酶原突变体(DscuPA-32K)中的应用

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生物工程学报 2001年第3期17卷 300-303页

作者：焦建伟俞梅敏茹炳根北京大学生命科学学院蛋白质工程国家重点实验室北京100871

将构建的一种具溶栓和抗栓双重功能尿激酶原突变体 (DscuPA 32K)基因 ,在大肠杆菌中进行表达。由于DscuPA 32K分子较大并且表达量较高 ,目的蛋白质基本以包涵体的形式存在。包涵体中的蛋白质是无活性的蛋白质 ,为了获得有活性的蛋白质 ... 详细信息

将构建的一种具溶栓和抗栓双重功能尿激酶原突变体 (DscuPA 32K)基因 ,在大肠杆菌中进行表达。由于DscuPA 32K分子较大并且表达量较高 ,目的蛋白质基本以包涵体的形式存在。包涵体中的蛋白质是无活性的蛋白质 ,为了获得有活性的蛋白质 ,就需要对包涵体进行变性及复性。尝试了一种新的凝胶色谱柱复性方法 ,并通过柱复性方法与常规的稀释复性方法进行了比较 ,发现柱复性方法明显优于稀释复性方法 ,具有成本低 ,效率高 ,并对目的蛋白质 (DscuPA 32K)进行了初步纯化等优点 ,尤其对酶这一类容易失活降解的蛋白质进行复性时。

关键词：低分子量尿激酶原包涵体复性基因突变体色谱柱洗脱溶栓药物

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Identification and Quantitative Analysis of Significantly Accumulated Proteins During the Arabidopsis Seedling De-etiolation Process

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Identification and Quantitative Analysis of Significantly Ac...

第四届拟南芥学术研讨会

作者： Bai-Chen Wang,Ying-Hong Pan,Da-Zhe Meng and Yu-Xian Zhu The National Laboratory of Protein Engineering and Plant Genetic Engineering,college of life sciences, peking university,beijing 100871,china

Proteomic analysis was performed on seedlings after different light treatments. A total of (1 350±31) protein spots was separated and visualized on each silver nitrate-stained two-dimensional gel using protein samples prepared from light-grown or etiolated seedlings with or without 6-9 h light treatment. Twenty-five protein spots (encoded by 19 genes) that were significantly accumulated upon light treatment were identified using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry method. Functional proteomics indicated that these proteins involved mainly in chloroplast development, energy metabolism, cell cycle progression and membrane electron transport. For 18 of the protein-coding genes we identified through an internet search, the transcript levels of 17 genes matched roughly with their protein content in etiolated and green seedlings, suggesting that these genes were regulated by light mainly at the transcrip-tional level. Despite a very significant increase in the amount of proteins upon light treatment, similar RNA levels were found in dark-grown or green seedlings for the carbonic anhydrase gene At3g05100, indicating a possible post-transcriptional regulatory mechanism. Elucidation of light-induced protein accumulation will undoubtedly enhance our understanding of plant photomorphogenesis.

关键词： Arabidopsis de-etiolation light two-dimensional eiectrophoresis